DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure of an Intron Lariat Spliceosome from Saccharomyces cerevisiae. Structure of an Intron Lariat Spliceosome from Saccharomyces cerevisiae. Cell 2017 imex curation Wan R., Yan C., Bai R., Lei J., Shi Y. shi-lab@tsinghua.edu.cn Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Wan R., Yan C., Bai R., Lei J., Shi Y. shi-lab@tsinghua.edu.cn Accepted 2021-SEP-10 AT 17:24 BST AT 17:24 BST by SIMONA Cell 2017 imex curation Structure of an Intron Lariat Spliceosome from Saccharomyces cerevisiae. Structure of an Intron Lariat Spliceosome from Saccharomyces cerevisiae. Cell 2017 imex curation Wan R., Yan C., Bai R., Lei J., Shi Y. shi-lab@tsinghua.edu.cn Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by staining molecular weight estimation by staining weight by staining Wan R., Yan C., Bai R., Lei J., Shi Y. shi-lab@tsinghua.edu.cn Accepted 2021-SEP-10 AT 17:24 BST AT 17:24 BST by SIMONA Cell 2017 imex curation msl1_yeast U2 small nuclear ribonucleoprotein B'' YIB9W YIR009W MSL1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVEPARKKQRIDRDTHHTVAEPVTEAKNTLYVSQLNEKINMQRLRVNLFLLFATFGEVLKVSMNFKKQRGQAFITMRTIDQASLAQISLNGERFFGKPLKVEFSKSETKTL 8FB0974FC30994AE syf2_yeast Pre-mRNA-splicing factor SYF2 SYF2 NTC31 Synthetic lethal with CDC40 protein 2 PRP19 complex protein 31 YGR129W protein protein yeast Saccharomyces cerevisiae Baker's yeast MDFYKLDEKLKELKRKRVDVSIKSRKLADREIQEVSANRKPRVYSMEDVNDADESVGDTESPEKEKAFHYTVQEYDAWERRHPQGKTGQSQRGGISYDQLAKLSYEKTLRNLATQTQNSSKQDSSADEEDNKNVPKKGRIGKVQKDTKTGKITIADDDKLVNKLAVSLQSESKKRYEARKRQMQNAKTLYGVESFINDKNKQFNEKLSRESKGSE C80B872FCD808E1D ruxg_yeast Small nuclear ribonucleoprotein G SNP2 YFL017Bw YFL017W-A SMX2 Sm protein G protein protein yeast Saccharomyces cerevisiae Baker's yeast MVSTPELKKYMDKKILLNINGSRKVAGILRGYDIFLNVVLDDAMEINGEDPANNHQLGLQTVIRGNSIISLEALDAI 5C6208B8C5A9AB33 5C6208B8C5A9AB33 rsmb_yeast Small nuclear ribonucleoprotein-associated protein B SMB1 Sm protein B YER029C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSKIQVAHSSRLANLIDYKLRVLTQDGRVYIGQLMAFDKHMNLVLNECIEERVPKTQLDKLRPRKDSKDGTTLNIKVEKRVLGLTILRGEQILSTVVEDKPLLSKKERLVRDKKEKKQAQKQTKLRKEKEKKPGKIAKPNTANAKHTSSNSREIAQPSSSRYNGGNDNIGANRSRFNNEAPPQTRKFQPPPGFKRK 0A1078D5AA241430 0A1078D5AA241430 cwc15_yeast Pre-mRNA-splicing factor CWC15 CWC15 Complexed with CEF1 protein 15 YDR163W YD8358.17 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTTSHRPQLEARSGAKAAAYTPTGIEHARLLPGHTTLKYRKFKEEENLRANCAQEDRSNDKSLEEAVMNEEKQDVVGSGNLQETRSEKDQKDSLQELLVTQKNKVEDKAELEGNEQLKGGNSSRRSWRKGTAFGRHKVTKETNIKEHATKKSASGYINDMTKSEYHQEFLHKHVR 6F8D838C169F0FF1 sn114_yeast Pre-mRNA-splicing factor SNU114 GIN10 YKL173W YKL637 SNU114 Growth inhibitory protein 10 114 kDa U5 small nuclear ribonucleoprotein component protein protein yeast Saccharomyces cerevisiae Baker's yeast MEGDDLFDEFGNLIGVDPFDSDEEESVLDEQEQYQTNTFEGSGNNNEIESRQLTSLGSKKELGISLEHPYGKEVEVLMETKNTQSPQTPLVEPVTERTKLQEHTIFTQLKKNIPKTRYNRDYMLSMANIPERIINVGVIGPLHSGKTSLMDLLVIDSHKRIPDMSKNVELGWKPLRYLDNLKQEIDRGLSIKLNGSTLLCTDLESKSRMINFLDAPGHVNFMDETAVALAASDLVLIVIDVVEGVTFVVEQLIKQSIKNNVAMCFVINKLDRLILDLKLPPMDAYLKLNHIIANINSFTKGNVFSPIDNNIIFASTKLGFTFTIKEFVSYYYAHSIPSSKIDDFTTRLWGSVYYHKGNFRTKPFENVEKYPTFVEFILIPLYKIFSYALSMEKDKLKNLLRSNFRVNLSQEALQYDPQPFLKHVLQLIFRQQTGLVDAITRCYQPFELFDNKTAHLSIPGKSTPEGTLWAHVLKTVDYGGAEWSLVRIYSGLLKRGDTVRILDTSQSESRQKRQLHDISKTETSNEDEDEDDETPSCEVEEIGLLGGRYVYPVHEAHKGQIVLIKGISSAYIKSATLYSVKSKEDMKQLKFFKPLDYITEAVFKIVLQPLLPRELPKLLDALNKISKYYPGVIIKVEESGEHVILGNGELYMDCLLYDLRASYAKIEIKISDPLTVFSESCSNESFASIPVSNSISRLGEENLPGLSISVAAEPMDSKMIQDLSRNTLGKGQNCLDIDGIMDNPRKLSKILRTEYGWDSLASRNVWSFYNGNVLINDTLPDEISPELLSKYKEQIIQGFYWAVKEGPLAEEPIYGVQYKLLSISVPSDVNIDVMKSQIIPLMKKACYVGLLTAIPILLEPIYEVDITVHAPLLPIVEELMKKRRGSRIYKTIKVAGTPLLEVRGQVPVIESAGFETDLRLSTNGLGMCQLYFWHKIWRKVPGDVLDKDAFIPKLKPAPINSLSRDFVMKTRRRKGISTGGFMSNDGPTLEKYISAELYAQLRENGLVP 5CE30CEFF154D013 5CE30CEFF154D013 slt11_yeast Pre-mRNA-splicing factor SLT11 ECM2 SLT11 YBR065C YBR0614 Synthetic lethality with U2 protein 11 Extracellular mutant protein 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNDEINEPPPNICEQCLGDEANIRMTKIPQGSECKICTLPFTLYHFKTSKRSNNIIKTLICVRCATQRNICQCCMLDSRWHIPIQLRDHLISLVNEENVMTEEAKNDMMKRFLSLKNVKLGGAQITSDPSEADNIVDKLKNILLRATSDGPSTPLIKNTTALYKNEKGANEVKNLEKYASVDISHILKKLPLNESFLKNPSTKSFFLYNIDASIPEWKITDTVSQLLGIKKWKDGNSLSLIVNHKAKCGGLRFQSSELGERFVSKISETLVTPKGLKRGVLLIDRFRIFIIPWSSGFSAASFGTNTAENIKLSLSLNKLIQLELGLSFPTKSTDNAKNDKKKTSKKVHKDRSKKSKPRANKLTI BF21E687817A42B4 BF21E687817A42B4 prp19_yeast Pre-mRNA-processing factor 19 PRP19 PSO4 RING-type E3 ubiquitin transferase PRP19 YLL036C protein protein yeast Saccharomyces cerevisiae Baker's yeast MLCAISGKVPRRPVLSPKSRTIFEKSLLEQYVKDTGNDPITNEPLSIEEIVEIVPSAQQASLTESTNSATLKANYSIPNLLTSLQNEWDAIMLENFKLRSTLDSLTKKLSTVMYERDAAKLVAAQLLMEKNEDSKDLPKSSQQAVAITREEFLQGLLQSSRDFVARGKLKAPKWPILKNLELLQAQNYSRNIKTFPYKELNKSMYYDKWVCMCRCEDGALHFTQLKDSKTITTITTPNPRTGGEHPAIISRGPCNRLLLLYPGNQITILDSKTNKVLREIEVDSANEIIYMYGHNEVNTEYFIWADNRGTIGFQSYEDDSQYIVHSAKSDVEYSSGVLHKDSLLLALYSPDGILDVYNLSSPDQASSRFPVDEEAKIKEVKFADNGYWMVVECDQTVVCFDLRKDVGTLAYPTYTIPEFKTGTVTYDIDDSGKNMIAYSNESNSLTIYKFDKKTKNWTKDEESALCLQSDTADFTDMDVVCGDGGIAAILKTNDSFNIVALTP A44D8C7DD6F64185 A44D8C7DD6F64185 ruxe_yeast Small nuclear ribonucleoprotein E SME1 YOR159C Sm protein E protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNKVKTKAMVPPINCIFNFLQQQTPVTIWLFEQIGIRIKGKIVGFDEFMNVVIDEAVEIPVNSADGKEDVEKGTPLGKILLKGDNITLITSAD B76D72DA8456F3B5 B76D72DA8456F3B5 bud31_yeast Pre-mRNA-splicing factor BUD31 BUD31 Bud site selection protein 31 CWC14 Complexed with CEF1 protein 14 YCR63W YCR903 YCR063W protein protein yeast Saccharomyces cerevisiae Baker's yeast MPRIKTRRSKPAPDGFEKIKPTLTDFEIQLRDAQKDKSSKLAAKSNEQLWEIMQLHHQRSRYIYTLYYKRKAISKDLYDWLIKEKYADKLLIAKWRKTGYEKLCCLRCIQKNETNNGSTCICRVPRAQLEEEARKKGTQVSFHQCVHCGCRGCASTD 70A8B15F8DA6F85D 70A8B15F8DA6F85D prp46_yeast Pre-mRNA-splicing factor PRP46 CWC1 PRP nineteen-associated complex protein 50 PRP19-associated complex protein 50 P2594 YPL151C PRP46 NTC50 Pre-mRNA-processing protein 46 Complexed with CEF1 protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDGNDHKVENLGDVDKFYSRIRWNNQFSYMATLPPHLQSEMEGQKSLLMRYDTYRKESSSFSGEGKKVTLQHVPTDFSEASQAVISKKDHDTHASAFVNKIFQPEVAEELIVNRYEKLLSQRPEWHAPWKLSRVINGHLGWVRCVAIDPVDNEWFITGSNDTTMKVWDLATGKLKTTLAGHVMTVRDVAVSDRHPYLFSVSEDKTVKCWDLEKNQIIRDYYGHLSGVRTVSIHPTLDLIATAGRDSVIKLWDMRTRIPVITLVGHKGPINQVQCTPVDPQVVSSSTDATVRLWDVVAGKTMKVLTHHKRSVRATALHPKEFSVASACTDDIRSWGLAEGSLLTNFESEKTGIINTLSINQDDVLFAGGDNGVLSFYDYKSGHKYQSLATREMVGSLEGERSVLCSTFDKTGLRLITGEADKSIKIWKQDETATKESEPGLAWNPNLSAKRF 0E888F566A5414BD 0E888F566A5414BD prp17_yeast Pre-mRNA-processing factor 17 CDC40 PRP17 SLU4 XRS2 YDR364C D9481.11 Cell division control protein 40 protein protein yeast Saccharomyces cerevisiae Baker's yeast MGLVDGYDTSSDSDLNFDEGKSVHEKKNGNLHEDTSYEPSSNNIHKRKSHFTKSELKRRRKTRKGDGPWGSWSSSDDETSQASETQKEDQDIFVHALAEDNLDSEQIEVEEVSHFYGKSEKDYQGRGYLYPPNDVDVDLREERISFRCYLPKKVIRNYPGHPEGTTALKFLPKTGHLILSGGNDHTIKIWDFYHDYECLRDFQGHNKPIKALRFTEDCQSFLSSSFDRSVKIWDTETGKVKTRLHLNSTPADVESRPTNPHEFIVGLSNSKILHYDDRVSENQGLVQTYDHHLSSILALKYFPDGSKFISSSEDKTVRIWENQINVPIKQISDTAQHSMPFLNVHPSQNYFCAQSMDNRIYSFSLKPKYKRHPKKIFKGHSSAGYGISLAFSGDGRYICSGDSKSRLFTWDWNTSRLLNNIKIPGNKPITQVDWHPQETSKVICSGAAGKIYVCD 40AED5DB35319147 40AED5DB35319147 prp45_yeast Pre-mRNA-processing protein 45 YAL032C PRP45 FUN20 protein protein yeast Saccharomyces cerevisiae Baker's yeast MFSNRLPPPKHSQGRVSTALSSDRVEPAILTDQIAKNVKLDDFIPKRQSNFELSVPLPTKAEIQECTARTKSYIQRLVNAKLANSNNRASSRYVTETHQAPANLLLNNSHHIEVVSKQMDPLLPRFVGKKARKVVAPTENDEVVPVLHMDGSNDRGEADPNEWKIPAAVSNWKNPNGYTVALERRVGKALDNENNTINDGFMKLSEALENADKKARQEIRSKMELKRLAMEQEMLAKESKLKELSQRARYHNGTPQTGAIVKPKKQTSTVARLKELAYSQGRDVSEKIILGAAKRSEQPDLQYDSRFFTRGANASAKRHEDQVYDNPLFVQQDIESIYKTNYEKLDEAVNVKSEGASGSHGPIQFTKAESDDKSDNYGA FE5A724186D7FE84 FE5A724186D7FE84 yju2_yeast Splicing factor YJU2 YKL095W YKL442 YJU2 CWC16 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSERKAINKYYPPDYNPLEAEKLSRKMAKKLKTMNKSHASIRLMTPFSMRCLECNEYIPKSRKFNGKKELLKEKYLDSIKIYRLTISCPRCANSIAFRTDPGNSDYVMEVGGVRNYVPQKPNDDLNAKTAVESIDETLQRLVREKEMEQNEKMGIKEQADDKMDLLEKRLAKIQQEQEDDEELENLRKKNLEMSQRAEMINRSKHAQQEKAVTTDDLDNLVDQVFDNHRQRTNKPGNNNDEKRTPLFNPTSTKGKIQKKSSVRTNPLGIVIKRGKSLK B57F3A173A2113FD B57F3A173A2113FD smd2_yeast Small nuclear ribonucleoprotein Sm D2 SMD2 YLR275W L9328.5 snRNP core protein D2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSQIIDRPKHELSRAELEELEEFEFKHGPMSLINDAMVTRTPVIISLRNNHKIIARVKAFDRHCNMVLENVKELWTEKKGKNVINRERFISKLFLRGDSVIVVLKTPVE 7287070B10E090C0 7287070B10E090C0 ntr2_yeast Pre-mRNA-splicing factor NTR2 YKR022C Nineteen complex-related protein 2 NTR2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MAIKKRNKIRLPSGSPEEVGIDGSAHKPMQQIKPLVSNDSEDDDNDICVLQPIKFKKVPKRDITFDGEQAIKEDNSHYEDLYHSKKNTNASTRNKDDLLILNMEDLMEGNHHLLSDSSEAGSSSEGEHISSIPTRGEIAKLKAQKSLSRRKISESDVTTERDYVKLLDSEDKREIMETIRLNGGLKRNNEKEITNFSDDEMQGFQDEMLALTDNQIAIQKDSKRKIIEKAINEVPYRTNEEWETQLLSKGNINKSNEKIITPLPVLFPDDDESGNSIERINEMVSKICLQRKKVEMRLQALEKTKIDLEKSKASLINKLIGN D7A601A46839244C syf1_yeast Pre-mRNA-splicing factor SYF1 SYF1 NTC90 Synthetic lethal with CDC40 protein 1 PRP19-associated complex protein 90 YDR416W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSAYIAMKGVITNVDENIRNDEDVAFEYEIQKTPQNILTWKRYIEYWKEEGRTDKQIRWLYERFCSQFVTDTSIWEDYIRWESTKEVVETSRIFWLFQRCLKSCVRDCDRICLSYLELAIEQYDLAMIRHALASSLMKMEREMHRKVWDPVIKFVEEKVLPLTQLDSTQEDEEESTDEAELINVLLVKGFTKGGFISEEISENGSRGDIWSSHILERYLKVAPQQKRNESLATLALTRDNITIKSVYEKYLPQDENSGKYLPSSELPFELNFNYLASLEKLGLDNQYEEFMRQMNGIYPDKWLFLILSLAKYYISRGRLDSCGDLLKKSLQQTLRYSDFDRIYNFYLLFEQECSQFILGKLKENDSKFFNQKDWTEKLQAHMATFESLINLYDIYLNDVALRQDSNLVETWMKRVSLQKSAAEKCNVYSEAILKIDPRKVGTPGSFGRLWCSYGDLYWRSNAISTARELWTQSLKVPYPYIEDLEEIYLNWADRELDKEGVERAFSILEDALHVPTNPEILLEKYKNGHRKIPAQTVLFNSLRIWSKYIDYLEAYCPKDANSSDKIFNKTKMAYNTVIDLRLITPAMAENFALFLQNHYEVMESFQVYEKTIPLFPPEIQYELWIEYLEVATSHQLSSLSPEHIRFLFEKALKNLCSNGIDCKTIFIAYSVFEERISGLISKSIEILRRGAVIGTVSVSTHLESRLQLWRMCISKAESTLGPSVTRELYQECIQILPNSKAVEFVIKFSDFESSIGETIRAREILAYGAKLLPPSRNTELWDSFEIFELKHGDKETYKDMLKMKKVLESNMLIDSASVSHEEGNINFVAAATSHAPNSHTLTQSTSSYSINPDEIELDI ED4368A624A40E7C ED4368A624A40E7C ru2a_yeast U2 small nuclear ribonucleoprotein A' YPL213W LEA1 Looks exceptionally like U2A protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKFTPSIVIDAPQYYVDHFNGKYNVDKCVILRDLQLETDSESMPSSLKHLTKPTHILDLTNNDLIMIPDLSRRDDIHTLLLGRNNIVEVDGRLLPMNVQNLTLSNNSIRRFEDLQRLRRAPRTLKNLTLIGNQVCHLANYREHVLRLVPHLETLDFQNVTAEERKSAMSFPRQADGDTLGPVNTAIRDNGSRDKTMEIMNLVVSKMTVERRNELKKQLAEATSLEEIARLEKLLSGGV 0E92A1922BFEDC25 sp382_yeast Pre-mRNA-splicing factor SPP382 NTR1 YLR424W SPP382 CCF8 Suppressor of PRP38 protein 2 Nineteen complex-related protein 1 CLF1 complex factor 8 protein protein yeast Saccharomyces cerevisiae Baker's yeast MEDSDSNTDKKFFFKKRRIDSYNYSDEEDNNSSMNSDMTYTNDALKTSSGNAPTISKLTKTYGIGAKLLSSMGYVAGKGLGKDGSGITTPIETQSRPMHNAGLGMFSNTNSSNYHSENEDYLSSEDEVVEGIEQVKFNKTSTEVLGEALLNDSGDMTIVRTLRELRLAGVQLPESILKELDPLNAVPKPKKDVVVEILQELLGIEKSLEAIRQRTSPLEVQVKEYYGQERLLSELEVTLRDESKHVSLYDKIGAILKLSDDELIDRLTSCLLRKELLIEFDLDHLEKPNDILDELTQIIELLAYRMDTTSKFLNRTQTTIFKVIYPKLKKFWEGFDMTKSKIDSAITLLLDFQQVLSFIGCKEHIMEEFVYPKLLQELDNWELHDEVDHVSPRIWVLDFMVLIDDKIKDTIVDKIEAKFFAYCKNWYHRESFCITNSDIIFIKELICERRYYKILCKEFLPKFLDELWERHNDPIYELEDWKEKQEWKEKDSGFFYFMKKLRSYTHYFHPKQYELMMRGTFNNINKILYQWHLYSTVEDLHKSKWWLNWLMNTVFEHSLPTEIELSEIRKSYNIFAMSHRYHLDKSTLDEDFDLRQGLRNLMETQVIDDISQSEQEPTYTVQNIPLGKVSSSFKDVVEDYCLEKGYLISKIPNRYTQLPYGRDQDCIVPLFEIRNGKKKMEVALKHDILWVEDSSGTFKPIYLWALDL 9986B547B9582338 9986B547B9582338 prp43_yeast Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43 PRP43 Helicase JA1 YGL120C protein protein yeast Saccharomyces cerevisiae Baker's yeast MGSKRRFSSEHPDPVETSIPEQAAEIAEELSKQHPLPSEEPLVHHDAGEFKGLQRHHTSAEEAQKLEDGKINPFTGREFTPKYVDILKIRRELPVHAQRDEFLKLYQNNQIMVFVGETGSGKTTQIPQFVLFDEMPHLENTQVACTQPRRVAAMSVAQRVAEEMDVKLGEEVGYSIRFENKTSNKTILKYMTDGMLLREAMEDHDLSRYSCIILDEAHERTLATDILMGLLKQVVKRRPDLKIIIMSATLDAEKFQRYFNDAPLLAVPGRTYPVELYYTPEFQRDYLDSAIRTVLQIHATEEAGDILLFLTGEDEIEDAVRKISLEGDQLVREEGCGPLSVYPLYGSLPPHQQQRIFEPAPESHNGRPGRKVVISTNIAETSLTIDGIVYVVDPGFSKQKVYNPRIRVESLLVSPISKASAQQRAGRAGRTRPGKCFRLYTEEAFQKELIEQSYPEILRSNLSSTVLELKKLGIDDLVHFDFMDPPAPETMMRALEELNYLACLDDEGNLTPLGRLASQFPLDPMLAVMLIGSFEFQCSQEILTIVAMLSVPNVFIRPTKDKKRADDAKNIFAHPDGDHITLLNVYHAFKSDEAYEYGIHKWCRDHYLNYRSLSAADNIRSQLERLMNRYNLELNTTDYESPKYFDNIRKALASGFFMQVAKKRSGAKGYITVKDNQDVLIHPSTVLGHDAEWVIYNEFVLTSKNYIRTVTSVRPEWLIEIAPAYYDLSNFQKGDVKLSLERIKEKVDRLNELKQGKNKKKSKHSKK DA8A085EC50FF430 DA8A085EC50FF430 cef1_yeast Pre-mRNA-splicing factor CEF1 CEF1 YMR213W YM8261.07 NTC85 PRP nineteen-associated complex protein 85 PRP19-associated complex protein 85 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPPVPIYVKGGVWTNVEDQILKAAVQKYGTHQWSKVASLLQKKTARQSELRWNEYLNPKLNFTEFSKEEDAQLLDLARELPNQWRTIADMMARPAQVCVERYNRLLESEDSGGAALSTGVTDLKAGDINPNAETQMARPDNGDLEDEEKEMLAEARARLLNTQGKKATRKIRERMLEESKRIAELQKRRELKQAGINVAIKKPKKKYGTDIDYNEDIVYEQAPMPGIYDTSTEDRQIKKKFEQFERKVNRKGLDGNKDKPSKKNKDKKRKHDENEHVEKAALGESTTLTDEYKKPKLILSAPGTKQGKVTYKKKLESKRQKLIEAQATGTVLTPKELLPHDSGQEDNERSNIKSGKQLKSRIRKFLVQMFASLPSPKNDFEIVLSEDEKEEDAEIAEYEKEFENERAMNEEDNFIEPPSQNDAPRVSLVAVPLAYSTLPIPEFKNNPQSAIDNKYNLLVANAINKEPHMVPEDTVDFLKEVESRMQHITQGRTSMKIQFKTAMPPTEVLLESIQSKVESIEQLQRKLQHVQPLEQQNNEMCSTLCHHSLPALIEGQRKYYADYYAYRQEIRSLEGRRKRLQAMLNSSSSI A4C417A8B330EA9C A4C417A8B330EA9C smd1_yeast Small nuclear ribonucleoprotein Sm D1 YGR074W SMD1 snRNP core protein D1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKLVNFLKKLRNEQVTIELKNGTTVWGTLQSVSPQMNAILTDVKLTLPQPRLNKLNSNGIAMASLYLTGGQQPTASDNIASLQYINIRGNTIRQIILPDSLNLDSLLVDQKQLNSLRRSGQIANDPSKKRRRDFGAPANKRPRRGL 9929F21EB9218447 9929F21EB9218447 clf1_yeast Pre-mRNA-splicing factor CLF1 CLF1 NTC77 YLR117C SYF3 L2952 Crooked neck-like factor 1 PRP19-associated complex protein 77 Synthetic lethal with CDC40 protein 3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDTLEPTAVDTHVSAEQILRDVYKKGQKARGSTNIDILDLEELREYQRRKRTEYEGYLKRNRLDMGQWIRYAQFEIEQHDMRRARSIFERALLVDSSFIPLWIRYIDAELKVKCINHARNLMNRAISTLPRVDKLWYKYLIVEESLNNVEIVRSLYTKWCSLEPGVNAWNSFVDFEIRQKNWNGVREIYSKYVMAHPQMQTWLKWVRFENRHGNTEFTRSVYSLAIDTVANLQNLQIWSDMEVAKLVNSFAHWEAAQQEYERSSALYQIAIEKWPSNQLLKAGLLDFEKQFGDINSIEETISYKRKMEYETILSNNAYDYDTWWLYLDLISESFPKQIMQTFEKAIVDSRPKELSKNVQWKRYIYLWMRYICYVELELENSLLEEELFQRLIDDIIPHKHFTFSKIWLMYAKFLIRHDDVPKARKILGKAIGLCPKAKTFKGYIELEVKLKEFDRVRKIYEKFIEFQPSDLQIWSQYGELEENLGDWDRVRGIYTIALDENSDFLTKEAKIVLLQKYITFETESQEFEKARKLYRRYLELNQYSPQSWIEFAMYQTSTPTEQQLLDLAKLQSENVDEDIEFEITDENKLEARKVFEEAIVFFKEKDDKQGRLSILEALKDYEETYGTELDQETVKKRFPKVIKKVRLQNGVEEEFVDYIFPDDIDDDKPKPSKFLELAKKWKQEQAL AE7D5EC5B3979E00 AE7D5EC5B3979E00 sn309_yeast Pre-mRNA-splicing factor SNT309 YPR101W SNT309 NTC25 Synergistic to PRP19 mutation protein 309 PRP19-associated complex protein 25 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDGLSFVDKGKIPDGYKNEIDQLVKKEFANIKREPVHPEIRGILAKRKGADNSVSTLTNALYTEYLKQRNNKKRRTPDFNDDDDTLFLEEYRRKYPRIDTSRYIPNESSEVSLLGIVDSYLKHQEIVLDTLLPQTVSNQWRINNDYIRQTCTIVEEMNIQQRKQINDLEIYRKRL B781CA65BC9A1918 B781CA65BC9A1918 ruxf_yeast Small nuclear ribonucleoprotein F SMX3 YPR182W P9705.4 Sm protein F protein protein yeast Saccharomyces cerevisiae Baker's yeast MSESSDISAMQPVNPKPFLKGLVNHRVGVKLKFNSTEYRGTLVSTDNYFNLQLNEAEEFVAGVSHGTLGEIFIRCNNVLYIRELPN 1640D3237597B2D8 1640D3237597B2D8 cwc2_yeast Pre-mRNA-splicing factor CWC2 CWC2 YDL209C D1041 NTC40 SLC3 Complexed with CEF1 protein 2 PRP19-associated complex protein 40 Synthetic lethal with CLF1 protein 3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTSWRDKSAKVQVKESELPSSIPAQTGLTFNIWYNKWSQGFAGNTRFVSPFALQPQLHSGKTRGDNDGQLFFCLFFAKGMCCLGPKCEYLHHIPDEEDIGKLALRTEVLDCFGREKFADYREDMGGIGSFRKKNKTLYVGGIDGALNSKHLKPAQIESRIRFVFSRLGDIDRIRYVESKNCGFVKFKYQANAEFAKEAMSNQTLLLPSDKEWDDRREGTGLLVKWANEDPDPAAQKRLQEELKLESLNMMVHLINNNTNSAGTEVNNKNNERLDRTFPEASVDNVKKRLLPLDNGMESDDFIEKLKKVKKNISRENISSKPSVGKLGGPLLDYLSSDED 371734A11DFDB339 371734A11DFDB339 prp8_yeast Pre-mRNA-splicing factor 8 PRP8 DBF3 DNA39 RNA8 SLT21 USA2 YHR165C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSGLPPPPPGFEEDSDLALPPPPPPPPGYEIEELDNPMVPSSVNEDTFLPPPPPPPSNFEINAEEIVDFTLPPPPPPPGLDELETKAEKKVELHGKRKLDIGKDTFVTRKSRKRAKKMTKKAKRSNLYTPKAEMPPEHLRKIINTHSDMASKMYNTDKKAFLGALKYLPHAILKLLENMPHPWEQAKEVKVLYHTSGAITFVNETPRVIEPVYTAQWSATWIAMRREKRDRTHFKRMRFPPFDDDEPPLSYEQHIENIEPLDPINLPLDSQDDEYVKDWLYDSRPLEEDSKKVNGTSYKKWSFDLPEMSNLYRLSTPLRDEVTDKNYYYLFDKKSFFNGKALNNAIPGGPKFEPLYPREEEEDYNEFNSIDRVIFRVPIRSEYKVAFPHLYNSRPRSVRIPWYNNPVSCIIQNDEEYDTPALFFDPSLNPIPHFIDNNSSLNVSNTKENGDFTLPEDFAPLLAEEEELILPNTKDAMSLYHSPFPFNRTKGKMVRAQDVALAKKWFLQHPDEEYPVKVKVSYQKLLKNYVLNELHPTLPTNHNKTKLLKSLKNTKYFQQTTIDWVEAGLQLCRQGHNMLNLLIHRKGLTYLHLDYNFNLKPTKTLTTKERKKSRLGNSFHLMRELLKMMKLIVDTHVQFRLGNVDAFQLADGIHYILNHIGQLTGIYRYKYKVMHQIRACKDLKHIIYYKFNKNLGKGPGCGFWQPAWRVWLNFLRGTIPLLERYIGNLITRQFEGRSNEIVKTTTKQRLDAYYDLELRNSVMDDILEMMPESIRQKKARTILQHLSEAWRCWKANIPWDVPGMPAPIKKIIERYIKSKADAWVSAAHYNRERIKRGAHVEKTMVKKNLGRLTRLWIKNEQERQRQIQKNGPEITPEEATTIFSVMVEWLESRSFSPIPFPPLTYKNDTKILVLALEDLKDVYASKVRLNASEREELALIEEAYDNPHDTLNRIKKYLLTQRVFKPVDITMMENYQNISPVYSVDPLEKITDAYLDQYLWYEADQRKLFPNWIKPSDSEIPPLLVYKWTQGINNLSEIWDVSRGQSAVLLETTLGEMAEKIDFTLLNRLLRLIVDPNIADYITAKNNVVINFKDMSHVNKYGLIRGLKFASFIFQYYGLVIDLLLLGQERATDLAGPANNPNEFMQFKSKEVEKAHPIRLYTRYLDRIYMLFHFEEDEGEELTDEYLAENPDPNFENSIGYNNRKCWPKDSRMRLIRQDVNLGRAVFWEIQSRVPTSLTSIKWENAFVSVYSKNNPNLLFSMCGFEVRILPRQRMEEVVSNDEGVWDLVDERTKQRTAKAYLKVSEEEIKKFDSRIRGILMASGSTTFTKVAAKWNTSLISLFTYFREAIVATEPLLDILVKGETRIQNRVKLGLNSKMPTRFPPAVFYTPKELGGLGMISASHILIPASDLSWSKQTDTGITHFRAGMTHEDEKLIPTIFRYITTWENEFLDSQRVWAEYATKRQEAIQQNRRLAFEELEGSWDRGIPRISTLFQRDRHTLAYDRGHRIRREFKQYSLERNSPFWWTNSHHDGKLWNLNAYRTDVIQALGGIETILEHTLFKGTGFNSWEGLFWEKASGFEDSMQFKKLTHAQRTGLSQIPNRRFTLWWSPTINRANVYVGFLVQLDLTGIFLHGKIPTLKISLIQIFRAHLWQKIHESIVFDICQILDGELDVLQIESVTKETVHPRKSYKMNSSAADITMESVHEWEVSKPSLLHETNDSFKGLITNKMWFDVQLRYGDYDSHDISRYVRAKFLDYTTDNVSMYPSPTGVMIGIDLAYNMYDAYGNWFNGLKPLIQNSMRTIMKANPALYVLRERIRKGLQIYQSSVQEPFLNSSNYAELFNNDIKLFVDDTNVYRVTVHKTFEGNVATKAINGCIFTLNPKTGHLFLKIIHTSVWAGQKRLSQLAKWKTAEEVSALVRSLPKEEQPKQIIVTRKAMLDPLEVHMLDFPNIAIRPTELRLPFSAAMSIDKLSDVVMKATEPQMVLFNIYDDWLDRISSYTAFSRLTLLLRALKTNEESAKMILLSDPTITIKSYHLWPSFTDEQWITIESQMRDLILTEYGRKYNVNISALTQTEIKDIILGQNIKAPSVKRQKMAELEAARSEKQNDEEAAGASTVMKTKTINAQGEEIVVVASADYESQTFSSKNEWRKSAIANTLLYLRLKNIYVSADDFVEEQNVYVLPKNLLKKFIEISDVKIQVAAFIYGMSAKDHPKVKEIKTVVLVPQLGHVGSVQISNIPDIGDLPDTEGLELLGWIHTQTEELKFMAASEVATHSKLFADKKRDCIDISIFSTPGSVSLSAYNLTDEGYQWGEENKDIMNVLSEGFEPTFSTHAQLLLSDRITGNFIIPSGNVWNYTFMGTAFNQEGDYNFKYGIPLEFYNEMHRPVHFLQFSELAGDEELEAEQIDVFS 8F4F6F89D34D3508 8F4F6F89D34D3508 cwc23_yeast Pre-mRNA-splicing factor CWC23 YGL128C G2861 CWC23 Complexed with CEF1 protein 23 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPGHELEDVINQRLNLYDVLELPTPLDVHTIYDDLPQIKRKYRTLALKYHPDKHPDNPSIIHKFHLLSTATNILTNADVRPHYDRWLIEFLRKTNDIERNKLIQKLEESESSTIPTTTPHPDLLQIQRHGELLRKLKHFNLPYGDWKHLNTQDQENASQHPYYDCSTLRIVLDNFLQSNNKSNCLSHLRNQVFITLSANEIYDIYFSERNNYSKDDSIIIYTVFDTPITAQHVFRNWSSGNLIPTVKDISPLIPLHYYSDFNLETELNDDIARLVSNEPILLD AA1D0894328CAEA5 AA1D0894328CAEA5 smd3_yeast Small nuclear ribonucleoprotein Sm D3 SMD3 YLR147C L9634.6 snRNP core protein D3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTMNGIPVKLLNEAQGHIVSLELTTGATYRGKLVESEDSMNVQLRDVIATEPQGAVTHMDQIFVRGSQIKFIVVPDLLKNAPLFKKNSSRPMPPIRGPKRR BB02A56C5AD70CF2 BB02A56C5AD70CF2 bud31-cdc40-6 1 10 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 3 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 20 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 17 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 24 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 31 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 5 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 27 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 11 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 6 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 22 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 18 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 21 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 30 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 14 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 16 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified TAP tag TAP tag tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 15 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 7 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 13 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 28 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 9 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 12 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 4 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 23 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 25 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 19 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 26 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 8 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 29 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified physical association physical association Fig 1, Fig S2 bud31-cdc40-7 2 10 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 3 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 20 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 17 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 24 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 31 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 5 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 27 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 11 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 6 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 22 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 18 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 21 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 30 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 14 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 16 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 15 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 7 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 13 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 28 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 9 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 12 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 4 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 23 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 25 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 19 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 26 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 8 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro 29 unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro physical association physical association Fig S1