MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it A Novel Human CAMK2Aalpha Mutation Disrupts Dendritic Morphology and Synaptic Transmission, and Causes ASD-Related Behaviors. A Novel Human CAMK2Aalpha Mutation Disrupts Dendritic Morphology and Synaptic Transmission, and Causes ASD-Related Behaviors. The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 imex curation Stephenson JR., Wang X., Perfitt TL., Parrish WP., Shonesy BC., Marks CR., Mortlock DP., Nakagawa T., Sutcliffe JS., Colbran RJ. Brain Disease - Neurological Diseases & Disorders from PMID: 3752148 (Arons et al., 2012) GFP-Shank3 is from rat origin. from PMID: 10764765 (Strack et al.) GST-GluN2B is from rat origin. from PMID: GST-beta2a (Abiria and Colbran, 2010) is from rat origin. It was no possible to retrieve origin species of GST-Densin IN. interactions involving this construct were not captured. Size-exclusion chromatography was not captured as no information about the multimer/monomer state of the protein is provided. from PMID: 3752148 (Arons et al., 2012) GFP-Shank3 is from rat origin. from PMID: 10764765 (Strack et al.) GST-GluN2B is from rat origin. from PMID: GST-beta2a (Abiria and Colbran, 2010) is from rat origin. It was no possible to retrieve origin species of GST-Densin IN. interactions involving this construct were not captured. Size-exclusion chromatography was not captured as no information about the multimer/monomer state of the protein is provided. roger.colbran@vanderbilt.edu Only protein-protein interactions imex curation in vitro In vitro array technology array technology tag fluorescence tag visualisation by fluorescence tag fluorescence Stephenson JR., Wang X., Perfitt TL., Parrish WP., Shonesy BC., Marks CR., Mortlock DP., Nakagawa T., Sutcliffe JS., Colbran RJ. Brain Disease - Neurological Diseases & Disorders from PMID: 3752148 (Arons et al., 2012) GFP-Shank3 is from rat origin. from PMID: 10764765 (Strack et al.) GST-GluN2B is from rat origin. from PMID: GST-beta2a (Abiria and Colbran, 2010) is from rat origin. It was no possible to retrieve origin species of GST-Densin IN. interactions involving this construct were not captured. Size-exclusion chromatography was not captured as no information about the multimer/monomer state of the protein is provided. from PMID: 3752148 (Arons et al., 2012) GFP-Shank3 is from rat origin. from PMID: 10764765 (Strack et al.) GST-GluN2B is from rat origin. from PMID: GST-beta2a (Abiria and Colbran, 2010) is from rat origin. It was no possible to retrieve origin species of GST-Densin IN. interactions involving this construct were not captured. Size-exclusion chromatography was not captured as no information about the multimer/monomer state of the protein is provided. roger.colbran@vanderbilt.edu Accepted 2021-OCT-08 AT 09:07 BST AT 09:07 BST by MGT The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Only protein-protein interactions imex curation imex curation A Novel Human CAMK2Aalpha Mutation Disrupts Dendritic Morphology and Synaptic Transmission, and Causes ASD-Related Behaviors. A Novel Human CAMK2Aalpha Mutation Disrupts Dendritic Morphology and Synaptic Transmission, and Causes ASD-Related Behaviors. The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 imex curation Stephenson JR., Wang X., Perfitt TL., Parrish WP., Shonesy BC., Marks CR., Mortlock DP., Nakagawa T., Sutcliffe JS., Colbran RJ. Brain Disease - Neurological Diseases & Disorders from PMID: 3752148 (Arons et al., 2012) GFP-Shank3 is from rat origin. from PMID: 10764765 (Strack et al.) GST-GluN2B is from rat origin. from PMID: GST-beta2a (Abiria and Colbran, 2010) is from rat origin. It was no possible to retrieve origin species of GST-Densin IN. interactions involving this construct were not captured. Size-exclusion chromatography was not captured as no information about the multimer/monomer state of the protein is provided. from PMID: 3752148 (Arons et al., 2012) GFP-Shank3 is from rat origin. from PMID: 10764765 (Strack et al.) GST-GluN2B is from rat origin. from PMID: GST-beta2a (Abiria and Colbran, 2010) is from rat origin. It was no possible to retrieve origin species of GST-Densin IN. interactions involving this construct were not captured. Size-exclusion chromatography was not captured as no information about the multimer/monomer state of the protein is provided. roger.colbran@vanderbilt.edu Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Stephenson JR., Wang X., Perfitt TL., Parrish WP., Shonesy BC., Marks CR., Mortlock DP., Nakagawa T., Sutcliffe JS., Colbran RJ. Brain Disease - Neurological Diseases & Disorders from PMID: 3752148 (Arons et al., 2012) GFP-Shank3 is from rat origin. from PMID: 10764765 (Strack et al.) GST-GluN2B is from rat origin. from PMID: GST-beta2a (Abiria and Colbran, 2010) is from rat origin. It was no possible to retrieve origin species of GST-Densin IN. interactions involving this construct were not captured. Size-exclusion chromatography was not captured as no information about the multimer/monomer state of the protein is provided. from PMID: 3752148 (Arons et al., 2012) GFP-Shank3 is from rat origin. from PMID: 10764765 (Strack et al.) GST-GluN2B is from rat origin. from PMID: GST-beta2a (Abiria and Colbran, 2010) is from rat origin. It was no possible to retrieve origin species of GST-Densin IN. interactions involving this construct were not captured. Size-exclusion chromatography was not captured as no information about the multimer/monomer state of the protein is provided. roger.colbran@vanderbilt.edu Accepted 2021-OCT-08 AT 09:07 BST AT 09:07 BST by MGT The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Only protein-protein interactions imex curation imex curation grm5_rat Metabotropic glutamate receptor 5 Grm5 Gprc1e Mglur5 protein protein rat Rattus norvegicus (Rat) Rat MVLLLILSVLLLKEDVRGSAQSSERRVVAHMPGDIIIGALFSVHHQPTVDKVHERKCGAVREQYGIQRVEAMLHTLERINSDPTLLPNITLGCEIRDSCWHSAVALEQSIEFIRDSLISSEEEEGLVRCVDGSSSFRSKKPIVGVIGPGSSSVAIQVQNLLQLFNIPQIAYSATSMDLSDKTLFKYFMRVVPSDAQQARAMVDIVKRYNWTYVSAVHTEGNYGESGMEAFKDMSAKEGICIAHSYKIYSNAGEQSFDKLLKKLRSHLPKARVVACFCEGMTVRGLLMAMRRLGLAGEFLLLGSDGWADRYDVTDGYQREAVGGITIKLQSPDVKWFDDYYLKLRPETNLRNPWFQEFWQHRFQCRLEGFAQENSKYNKTCNSSLTLRTHHVQDSKMGFVINAIYSMAYGLHNMQMSLCPGYAGLCDAMKPIDGRKLLDSLMKTNFTGVSGDMILFDENGDSPGRYEIMNFKEMGKDYFDYINVGSWDNGELKMDDDEVWSKKNNIIRSVCSEPCEKGQIKVIRKGEVSCCWTCTPCKENEYVFDEYTCKACQLGSWPTDDLTGCDLIPVQYLRWGDPEPIAAVVFACLGLLATLFVTVIFIIYRDTPVVKSSSRELCYIILAGICLGYLCTFCLIAKPKQIYCYLQRIGIGLSPAMSYSALVTKTNRIARILAGSKKKICTKKPRFMSACAQLVIAFILICIQLGIIVALFIMEPPDIMHDYPSIREVYLICNTTNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITMCFSVSLSATVALGCMFVPKVYIILAKPERNVRSAFTTSTVVRMHVGDGKSSSAASRSSSLVNLWKRRGSSGETLRYKDRRLAQHKSEIECFTPKGSMGNGGRATMSSSNGKSVTWAQNEKSTRGQHLWQRLSVHINKKENPNQTAVIKPFPKSTENRGPGAAAGGGSGPGVAGAGNAGCTATGGPEPPDAGPKALYDVAEAEESFPAAARPRSPSPISTLSHLAGSAGRTDDDAPSLHSETAARSSSSQGSLMEQISSVVTRFTANISELNSMMLSTAATPGPPGTPICSSYLIPKEIQLPTTMTTFAEIQPLPAIEVTGGAQGATGVSPAQETPTGAESAPGKPDLEELVALTPPSPFRDSVDSGSTTPNSPVSESALCIPSSPKYDTLIIRDYTQSSSSL 99CA51E9E11C1EA4 shan3_rat SH3 and multiple ankyrin repeat domains protein 3 Shank3 Proline-rich synapse-associated protein 2 SPANK-2 Prosap2 protein protein rat Rattus norvegicus (Rat) Rat MDGPGASAVVVRVGIPDLQQTKCLRLDPTAPVWAAKQRVLCALNHSLQDALNYGLFQPPSRGRAGKFLDEERLLQDYPPNLDTPLPYLEFRYKRRVYAQNLIDDKQFAKLHTKANLKKFMDYVQLHSTDKVARLLDKGLDPNFHDPDSGECPLSLAAQLDNATDLLKVLRNGGAHLDFRTRDGLTAVHCATRQRNAGALTTLLDLGASPDYKDSRGLTPLYHSALGGGDALCCELLLHDHAQLGTTDENGWQEIHQACRFGHVQHLEHLLFYGANMGAQNASGNTALHICALYNQESCARVLLFRGANKDVRNYNSQTAFQVAIIAGNFELAEVIKTHKDSDVVPFRETPSYAKRRRLAGPSGLASPRPLQRSASDINLKGDQPAASPGPTLRSLPHQLLLQRLQEEKDRDRDGEQENDISGPSAGRGGHSKISPSGPGGSGPAPGPGPASPAPPAPPPRGPKRKLYSAVPGRKFIAVKAHSPQGEGEIPLHRGEAVKVLSIGEGGFWEGTVKGRTGWFPADCVEEVQMRQYDTRHETREDRTKRLFRHYTVGSYDSLTSHSDYVIDDKVAILQKRDHEGFGFVLRGAKAETPIEEFTPTPAFPALQYLESVDVEGVAWKAGLRTGDFLIEVNGVNVVKVGHKQVVGLIRQGGNRLVMKVVSVTRKPEEDSARRRAPPPPKRAPSTTLTLRSKSMTAELEELASIRRRKGEKLDEILAVAAEPTLRPDIADADSRAATVKQRPTSRRITPAEISSLFERQGLPGPEKLPGSLRKGIPRTKSVGEDEKLASLLEGRFPRSTSMQDTVREGRGIPPPPQTAPPPPPAPYYFDSGPPPTFSPPPPPPGRAYDTVRSSFKPGLEARLGAGAAGLYDSGTPLGPLPYPERQKRARSMIILQDSAPEVGDVPRPAPAATPPERPKRRPRPSGPDSPYANLGAFSASLFAPSKPQRRKSPLVKQLQVEDAQERAALAVGSPGPVGGSFAREPSPTHRGPRPGGLDYSSGEGLGLTFGGPSPGPVKERRLEERRRSTVFLSVGAIEGSPPSADLPSLQPSRSIDERLLGTGATTGRDLLLPSPVSALKPLVGGPSLGPSGSTFIHPLTGKPLDPSSPLALALAARERALASQTPSRSPTPVHSPDADRPGPLFVDVQTRDSERGPLASPAFSPRSPAWIPVPARREAEKPTREERKSPEDKKSMILSVLDTSLQRPAGLIVVHATSNGQEPNRLGAEEERPGTPELAPTPMQAAAVAEPMPSPRAQPPGSIPADPGPGQGSSEEEPELVFAVNLPPAQLSSSDEETREELARIGLVPPPEEFANGILLATPPPGPGPLPTTVPSPASGKPSSELPPAPESAADSGVEEADTRSSSDPHLETTSTISTVSSMSTLSSESGELTDTHTSFADGHTFLLEKPPVPPKPKLKSPLGKGPVTFRDPLLKQSSDSELMAQQHHATSTGLTSAAGPARPRYLFQRRSKLWGDPVESRGLPGPEDDKPTVISELSSRLQQLNKDTRSLGEEPVGGLGSLLDPAKKSPIAAARCAVVPSAGWLFSSLGELSTISAQRSPGGPGGGASYSVRPSGRYPVARRAPSPVKPASLERVEGLGAGVGGAGRPFGLTPPTILKSSSLSIPHEPKEVRFVVRSVSARSRSPSPSPLPSPSPGSGPSAGPRRPFQQKPLQLWSKFDVGDWLESIHLGEHRDRFEDHEIEGAHLPALTKEDFVELGVTRVGHRMNIERALRQLDGS A9F486E893EA5AA0 cacb2_rat Voltage-dependent L-type calcium channel subunit beta-2 Cacnb2 Cacnlb2 Calcium channel voltage-dependent subunit beta 2 protein protein rat Rattus norvegicus (Rat) Rat MVQSDTSKSPPIAAVAQESQMELLESAAPAGALGAQSYGKGARRKNRFKGSDDSTSSDTTSNSFVRQGSADSYTSRPSDSDVSLEEDREAVRREAERQAQAQLEKAKTKPVAFAVRTNVRYSAAQEDDVPVPGMAISFEAKDFLHVKEKFNNDWWIGRLVKEGCEIGFIPSPVKLENMRLQHEQRAKQGKFYSSKSGGNSSSSLGDIVPSSRKSTPPSSAIDIDATGLDAEENDIPANHRSPKPSANSVTSPHSKEKRMPFFKKTEHTPPYDVVPSMRPVVLVGPSLKGYEVTDMMQKALFDFLKHRFEGRISITRVTADISLAKRSVLNNPSKHAIIERSNTRSSLAEVQSEIERIFELARTLQLVVLDADTINHPAQLSKTSLAPIIVYVKISSPKVLQRLIKSRGKSQAKHLNVQMVAADKLAQCPPQESFDVILDENQLEDACEHLADYLEAYWKATHPPSSNLPNPLLSRTLATSTLPLSPTLASNSQGSQGDQRTDRSAPRSASQAEEEPCLEPVKKSQHRSSSATHQNHRSGTGRGLSRQETFDSETQESRDSAYVEPKEDYSHEHVDRYVPHREHNHREESHSSNGHRHREPRHRTRDMGRDQDHNECSKQRSRHKSKDRYCDKEGEVISKRRSEAGEWNRDVYIRQ 0BD9B42FD4CDF52A nmde2_rat Glutamate receptor ionotropic, NMDA 2B Grin2b Glutamate [NMDA] receptor subunit epsilon-2 N-methyl D-aspartate receptor subtype 2B protein protein rat Rattus norvegicus (Rat) Rat MKPSAECCSPKFWLVLAVLAVSGSKARSQKSPPSIGIAVILVGTSDEVAIKDAHEKDDFHHLSVVPRVELVAMNETDPKSIITRICDLMSDRKIQGVVFADDTDQEAIAQILDFISAQTLTPILGIHGGSSMIMADKDESSMFFQFGPSIEQQASVMLNIMEEYDWYIFSIVTTYFPGYQDFVNKIRSTIENSFVGWELEEVLLLDMSLDDGDSKIQNQLKKLQSPIILLYCTKEEATYIFEVANSVGLTGYGYTWIVPSLVAGDTDTVPSEFPTGLISVSYDEWDYGLPARVRDGIAIITTAASDMLSEHSFIPEPKSSCYNTHEKRIYQSNMLNRYLINVTFEGRNLSFSEDGYQMHPKLVIILLNKERKWERVGKWKDKSLQMKYYVWPRMCPETEEQEDDHLSIVTLEEAPFVIVESVDPLSGTCMRNTVPCQKRIISENKTDEEPGYIKKCCKGFCIDILKKISKSVKFTYDLYLVTNGKHGKKINGTWNGMIGEVVMKRAYMAVGSLTINEERSEVVDFSVPFIETGISVMVSRSNGTVSPSAFLEPFSADVWVMMFVMLLIVSAVAVFVFEYFSPVGYNRCLADGREPGGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEYVDQVSGLSDKKFQRPNDFSPPFRFGTVPNGSTERNIRNNYAEMHAYMGKFNQRGVDDALLSLKTGKLDAFIYDAAVLNYMAGRDEGCKLVTIGSGKVFASTGYGIAIQKDSGWKRQVDLAILQLFGDGEMEELEALWLTGICHNEKNEVMSSQLDIDNMAGVFYMLGAAMALSLITFICEHLFYWQFRHCFMGVCSGKPGMVFSISRGIYSCIHGVAIEERQSVMNSPTATMNNTHSNILRLLRTAKNMANLSGVNGSPQSALDFIRRESSVYDISEHRRSFTHSDCKSYNNPPCEENLFSDYISEVERTFGNLQLKDSNVYQDHYHHHHRPHSIGSTSSIDGLYDCDNPPFTTQPRSISKKPLDIGLPSSKHSQLSDLYGKFSFKSDRYSGHDDLIRSDVSDISTHTVTYGNIEGNAAKRRKQQYKDSLKKRPASAKSRREFDEIELAYRRRPPRSPDHKRYFRDKEGLRDFYLDQFRTKENSPHWEHVDLTDIYKERSDDFKRDSVSGGGPCTNRSHLKHGTGEKHGVVGGVPAPWEKNLTNVDWEDRSGGNFCRSCPSKLHNYSSTVAGQNSGRQACIRCEACKKAGNLYDISEDNSLQELDQPAAPVAVTSNASSTKYPQSPTNSKAQKKNRNKLRRQHSYDTFVDLQKEEAALAPRSVSLKDKGRFMDGSPYAHMFEMPAGESSFANKSSVPTAGHHHNNPGSGYMLSKSLYPDRVTQNPFIPTFGDDQCLLHGSKSYFFRQPTVAGASKTRPDFRALVTNKPVVVTLHGAVPGRFQKDICIGNQSNPCVPNNKNPRAFNGSSNGHVYEKLSSIESDV DIP protein Q00960 original sequence version: 102 DIP protein Q00960 original sequence version: 113 DIP protein Q00960 original sequence version: 148 DIP protein Q00960 original sequence version: 158 AEF8B9DF3C1B0D5D kcc2a_mouse Calcium/calmodulin-dependent protein kinase type II subunit alpha Camk2a protein protein mouse Mus musculus Mouse MATITCTRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWISHRSTVASCMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSGGKSGGNKKNDGVKESSESTNTTIEDEDTKVRKQEIIKVTEQLIEAISNGDFESYTKMCDPGMTAFEPEALGNLVEGLDFHRFYFENLWSRNSKPVHTTILNPHIHLMGDESACIAYIRITQYLDAGGIPRTAQSEETRVWHRRDGKWQIVHFHRSGAPSVLPH DIP protein P11798 original sequence version: 97 306F416CCE9B5F62 shank3-camk2a-1 2 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed P11798:p.Glu183Val mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain E V 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 3A camkii-mglu5-1 1 3 unspecified role unspecified role bait bait purified purified n-n gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate mApple tag fluorescent prot tag fluorescent protein tag fluorescent prot tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P11798:p.Glu183Val mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain E V human-293ft Homo sapiens HEK293FT cell line 293ft HEK293FT cell line physical association physical association Fig. 3B grm5-camk2a-1 1 6 unspecified role unspecified role bait bait purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate mApple tag fluorescent prot tag fluorescent protein tag fluorescent prot tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P11798:p.Glu183Val mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain E V human-293ft Homo sapiens HEK293FT cell line 293ft HEK293FT cell line physical association physical association Fig. 3B grin2b-camk2a-7 1 5 unspecified role unspecified role bait bait purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate mApple tag fluorescent prot tag fluorescent protein tag fluorescent prot tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P11798:p.Glu183Val mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain E V human-293ft Homo sapiens HEK293FT cell line 293ft HEK293FT cell line physical association physical association Fig. 3B