IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Cooperation meets competition in microRNA-mediated DMPK transcript regulation. Cooperation meets competition in microRNA-mediated DMPK transcript regulation. Nucleic acids research 2015 imex curation Koscianska E., Witkos TM., Kozlowska E., Wojciechowska M., Krzyzosiak WJ. edytak@ibch.poznan.pl Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag luciferase tag visualisation by luciferase assay tag luciferase Koscianska E., Witkos TM., Kozlowska E., Wojciechowska M., Krzyzosiak WJ. edytak@ibch.poznan.pl Accepted 2021-JUN-30 AT 16:53 BST AT 16:53 BST by ORCHARD Nucleic acids research 2015 Only protein-protein interactions imex curation imex curation Cooperation meets competition in microRNA-mediated DMPK transcript regulation. Cooperation meets competition in microRNA-mediated DMPK transcript regulation. Nucleic acids research 2015 imex curation Koscianska E., Witkos TM., Kozlowska E., Wojciechowska M., Krzyzosiak WJ. edytak@ibch.poznan.pl Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag luciferase tag visualisation by luciferase assay tag luciferase Koscianska E., Witkos TM., Kozlowska E., Wojciechowska M., Krzyzosiak WJ. I have not added here the interaction with DMPK CUG repeats (20 CTG are contained in the wt transcript) because it is significative only with miR15/16 used together, I do not think I can annotate this (Fig 6) edytak@ibch.poznan.pl Accepted 2021-JUN-30 AT 16:53 BST AT 16:53 BST by ORCHARD Nucleic acids research 2015 Only protein-protein interactions imex curation imex curation hsa-mir-148a 3p microrna hsa-miR-148a-3p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA human Homo sapiens Human UCAGUGCACUACAGAACUUUGU hsa-mir-1-3p microRNA hsa-miR-1-3p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA human Homo sapiens Human UGGAAUGUAAAGAAGUAUGUAU mrna_dmpk DM1 protein kinase mRNA mrna messenger rna mRNA mrna human Homo sapiens Human AGAGAGGCCCAGGGGACAGCCAGGGACAGGCAGACATGCAGCCAGGGCTCCAGGGCCTGGACAGGGGCTGCCAGGCCCTGTGACAGGAGGACCCCGAGCCCCCGGCCCGGGGAGGGGCCATGGTGCTGCCTGTCCAACATGTCAGCCGAGGTGCGGCTGAGGCGGCTCCAGCAGCTGGTGTTGGACCCGGGCTTCCTGGGGCTGGAGCCCCTGCTCGACCTTCTCCTGGGCGTCCACCAGGAGCTGGGCGCCTCCGAACTGGCCCAGGACAAGTACGTGGCCGACTTCTTGCAGTGGGCGGAGCCCATCGTGGTGAGGCTTAAGGAGGTCCGACTGCAGAGGGACGACTTCGAGATTCTGAAGGTGATCGGACGCGGGGCGTTCAGCGAGGTAGCGGTAGTGAAGATGAAGCAGACGGGCCAGGTGTATGCCATGAAGATCATGAACAAGTGGGACATGCTGAAGAGGGGCGAGGTGTCGTGCTTCCGTGAGGAGAGGGACGTGTTGGTGAATGGGGACCGGCGGTGGATCACGCAGCTGCACTTCGCCTTCCAGGATGAGAACTACCTGTACCTGGTCATGGAGTATTACGTGGGCGGGGACCTGCTGACACTGCTGAGCAAGTTTGGGGAGCGGATTCCGGCCGAGATGGCGCGCTTCTACCTGGCGGAGATTGTCATGGCCATAGACTCGGTGCACCGGCTTGGCTACGTGCACAGGGACATCAAACCCGACAACATCCTGCTGGACCGCTGTGGCCACATCCGCCTGGCCGACTTCGGCTCTTGCCTCAAGCTGCGGGCAGATGGAACGGTGCGGTCGCTGGTGGCTGTGGGCACCCCAGACTACCTGTCCCCCGAGATCCTGCAGGCTGTGGGCGGTGGGCCTGGGACAGGCAGCTACGGGCCCGAGTGTGACTGGTGGGCGCTGGGTGTATTCGCCTATGAAATGTTCTATGGGCAGACGCCCTTCTACGCGGATTCCACGGCGGAGACCTATGGCAAGATCGTCCACTACAAGGAGCACCTCTCTCTGCCGCTGGTGGACGAAGGGGTCCCTGAGGAGGCTCGAGACTTCATTCAGCGGTTGCTGTGTCCCCCGGAGACACGGCTGGGCCGGGGTGGAGCAGGCGACTTCCGGACACATCCCTTCTTCTTTGGCCTCGACTGGGATGGTCTCCGGGACAGCGTGCCCCCCTTTACACCGGATTTCGAAGGTGCCACCGACACATGCAACTTCGACTTGGTGGAGGACGGGCTCACTGCCATGGTGAGCGGGGGCGGGGAGACACTGTCGGACATTCGGGAAGGTGCGCCGCTAGGGGTCCACCTGCCTTTTGTGGGCTACTCCTACTCCTGCATGGCCCTCAGGGACAGTGAGGTCCCAGGCCCCACACCCATGGAACTGGAGGCCGAGCAGCTGCTTGAGCCACACGTGCAAGCGCCCAGCCTGGAGCCCTCGGTGTCCCCACAGGATGAAACAGCTGAAGTGGCAGTTCCAGCGGCTGTCCCTGCGGCAGAGGCTGAGGCCGAGGTGACGCTGCGGGAGCTCCAGGAAGCCCTGGAGGAGGAGGTGCTCACCCGGCAGAGCCTGAGCCGGGAGATGGAGGCCATCCGCACGGACAACCAGAACTTCGCCAGTCAACTACGCGAGGCAGAGGCTCGGAACCGGGACCTAGAGGCACACGTCCGGCAGTTGCAGGAGCGGATGGAGTTGCTGCAGGCAGAGGGAGCCACAGCTGTCACGGGGGTCCCCAGTCCCCGGGCCACGGATCCACCTTCCCATCTAGATGGCCCCCCGGCCGTGGCTGTGGGCCAGTGCCCGCTGGTGGGGCCAGGCCCCATGCACCGCCGCCACCTGCTGCTCCCTGCCAGGGTCCCTAGGCCTGGCCTATCGGAGGCGCTTTCCCTGCTCCTGTTCGCCGTTGTTCTGTCTCGTGCCGCCGCCCTGGGCTGCATTGGGTTGGTGGCCCACGCCGGCCAACTCACCGCAGTCTGGCGCCGCCCAGGAGCCGCCCGCGCTCCCTGAACCCTAGAACTGTCTTCGACTCCGGGGCCCCGTTGGAAGACTGAGTGCCCGGGGCACGGCACAGAAGCCGCGCCCACCGCCTGCCAGTTCACAACCGCTCCGAGCGTGGGTCTCCGCCCAGCTCCAGTCCTGTGATCCGGGCCCGCCCCCTAGCGGCCGGGGAGGGAGGGGCCGGGTCCGCGGCCGGCGAACGGGGCTCGAAGGGTCCTTGTAGCCGGGAATGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGGGGGGATCACAGACCATTTCTTTCTTTCGGCCAGGCTGAGGCCCTGACGTGGATGGGCAAACTGCAGGCCTGGGAAGGCAGCAAGCCGGGCCGTCCGTGTTCCATCCTCCACGCACCCCCACCTATCGTTGGTTCGCAAAGTGCAAAGCTTTCTTGTGCATGACGCCCTGCTCTGGGGAGCGTCTGGCGCGATCTCTGCCTGCTTACTCGGGAAATTTGCTTTTGCCAAACCCGCTTTTTCGGGGATCCCGCGCCCCCCTCCTCACTTGCGCTGCTCTCGGAGCCCCAGCCGGCTCCGCCCGCTTCGGCGGTTTGGATATTTATTGACCTCGTCCTCCGACTCGCTGACAGGCTACAGGACCCCCAACAACCCCAATCCACGTTTTGGATGCACTGAGACCCCGACATTCCTCGGTATTTATTGTCTGTCCCCACCTAGGACCCCCACCCCCGACCCTCGCGAATAAAAGGCCCTCCATCTGCCCAAA hsa-mir-206 mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA human Homo sapiens Human UGGAAUGUAAGGAAGUGUGUGG hsa_mir_206-mrna_dmpk-1 2 6 predetermined predetermined participant predetermined tag luciferase tag visualisation by luciferase assay tag luciferase regulator regulator modulator regulator unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell 5 regulator target regulator target regulator target modulator target unspecified role unspecified role tag luciferase tag luciferase tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ENST00000291270:r.2718_2720delinsctg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain ATT CTG physical association physical association Fig 2 B, Suppl Tab 2 hsa_mir_148a 3p-mrna_dmpk-1 2 3 predetermined predetermined participant predetermined tag luciferase tag visualisation by luciferase assay tag luciferase regulator regulator modulator regulator unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell 5 regulator target regulator target regulator target modulator target unspecified role unspecified role tag luciferase tag luciferase tag 3' range 3' range 3' range 3' range ENST00000291270:r.2704_2706delinsctg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 2 B, Suppl tab 2 hsa_mir_206-mrna_dmpk-3 1 6 inferred by author inferred by author modeled by author modelled by author tag luciferase tag visualisation by luciferase assay tag luciferase regulator regulator modulator regulator unspecified role unspecified role living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 5 regulator target regulator target regulator target modulator target unspecified role unspecified role tag luciferase tag luciferase tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ENST00000291270:r.2718_2720delinsctg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain ATT CTG physical association physical association Suppl Fig 1 hsa_mir_148a 3p-mrna_dmpk-2 1 3 inferred by author inferred by author modeled by author modelled by author tag luciferase tag visualisation by luciferase assay tag luciferase regulator regulator modulator regulator unspecified role unspecified role living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 5 regulator target regulator target regulator target modulator target unspecified role unspecified role tag luciferase tag luciferase tag 3' range 3' range 3' range 3' range ENST00000291270:r.2704_2706delinsctg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain physical association physical association Supp. Fig. 1, Suppl tab 2 hsa_mir_1_3p-mrna_dmpk-1 2 4 predetermined predetermined participant predetermined tag luciferase tag visualisation by luciferase assay tag luciferase regulator regulator modulator regulator unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell 5 regulator target regulator target regulator target modulator target unspecified role unspecified role tag luciferase tag luciferase tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ENST00000291270:r.2718_2720delinsctg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain ATT CTG physical association physical association Fig 3 A B