IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Pontin, a new mutant p53-binding protein, promotes gain-of-function of mutant p53. Pontin, a new mutant p53-binding protein, promotes gain-of-function of mutant p53. Cell death and differentiation 2015 imex curation Zhao Y., Zhang C., Yue X., Li X., Liu J., Yu H., Belyi VA., Yang Q., Feng Z., Hu W. wh221@cinj.rutgers.edu, fengzh@cinj.rutgers.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Zhao Y., Zhang C., Yue X., Li X., Liu J., Yu H., Belyi VA., Yang Q., Feng Z., Hu W. H1299 P53 KO cells were used in this experiment. wh221@cinj.rutgers.edu, fengzh@cinj.rutgers.edu Accepted 2021-APR-13 AT 11:24 BST AT 11:24 BST by MGT Cell death and differentiation 2015 Only protein-protein interactions imex curation imex curation Pontin, a new mutant p53-binding protein, promotes gain-of-function of mutant p53. Pontin, a new mutant p53-binding protein, promotes gain-of-function of mutant p53. Cell death and differentiation 2015 imex curation Zhao Y., Zhang C., Yue X., Li X., Liu J., Yu H., Belyi VA., Yang Q., Feng Z., Hu W. wh221@cinj.rutgers.edu, fengzh@cinj.rutgers.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhao Y., Zhang C., Yue X., Li X., Liu J., Yu H., Belyi VA., Yang Q., Feng Z., Hu W. wh221@cinj.rutgers.edu, fengzh@cinj.rutgers.edu Accepted 2021-APR-13 AT 11:24 BST AT 11:24 BST by MGT Cell death and differentiation 2015 Only protein-protein interactions imex curation imex curation Pontin, a new mutant p53-binding protein, promotes gain-of-function of mutant p53. Pontin, a new mutant p53-binding protein, promotes gain-of-function of mutant p53. Cell death and differentiation 2015 imex curation Zhao Y., Zhang C., Yue X., Li X., Liu J., Yu H., Belyi VA., Yang Q., Feng Z., Hu W. wh221@cinj.rutgers.edu, fengzh@cinj.rutgers.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-thymic lymphoma Mouse thymic lymphoma thymic lymphoma Thymic lymphoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot test mol mole test2 Zhao Y., Zhang C., Yue X., Li X., Liu J., Yu H., Belyi VA., Yang Q., Feng Z., Hu W. wh221@cinj.rutgers.edu, fengzh@cinj.rutgers.edu Accepted 2021-APR-13 AT 11:24 BST AT 11:24 BST by MGT Cell death and differentiation 2015 Only protein-protein interactions imex curation imex curation Pontin, a new mutant p53-binding protein, promotes gain-of-function of mutant p53. Pontin, a new mutant p53-binding protein, promotes gain-of-function of mutant p53. Cell death and differentiation 2015 imex curation Zhao Y., Zhang C., Yue X., Li X., Liu J., Yu H., Belyi VA., Yang Q., Feng Z., Hu W. wh221@cinj.rutgers.edu, fengzh@cinj.rutgers.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Zhao Y., Zhang C., Yue X., Li X., Liu J., Yu H., Belyi VA., Yang Q., Feng Z., Hu W. wh221@cinj.rutgers.edu, fengzh@cinj.rutgers.edu Accepted 2021-APR-13 AT 11:24 BST AT 11:24 BST by MGT Cell death and differentiation 2015 Only protein-protein interactions imex curation imex curation Pontin, a new mutant p53-binding protein, promotes gain-of-function of mutant p53. Pontin, a new mutant p53-binding protein, promotes gain-of-function of mutant p53. Cell death and differentiation 2015 imex curation Zhao Y., Zhang C., Yue X., Li X., Liu J., Yu H., Belyi VA., Yang Q., Feng Z., Hu W. wh221@cinj.rutgers.edu, fengzh@cinj.rutgers.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Zhao Y., Zhang C., Yue X., Li X., Liu J., Yu H., Belyi VA., Yang Q., Feng Z., Hu W. Full primers mapping for this experiment (run on 18.02.2021): STMN1: ENSG00000117632 SREBP-binding site: 1:25906409-25906736 F: 5′-GAGAATGGGGAGCTGGTTCG-3′ 1:25906717-25906736:- R: 5′-GACCACACTCTGAGCACCAA-3′ 1:25906409-25906428:+ Coding sequence (negative control): 1:25885649-25885808 F: 5′-GTCTCCACTCTGTTCCTGCC-3′ 1:25885789-25885808:- R: 5′-AGGATCCTCTTGGCCAGACT-3′ 1:25885649-25885668:+ RIPK1: ENSG00000137275 6:3064454-3064791 F: 5′-ATCCGAGCACTCTTACCCATTCTA-3′ 6:3064454-3064477:+ R: 5′-GTCACCTCTTCGCAAACTCCTG-3′ 6:3064770-3064791:- ITPR1: ENSG00000150995 3:4493661-4493986 F: 5′-CAGAAGTTTTTGCCCCGCTC-3′ 3:4493661-4493680:+ R: 5′-GGAGCGCAAGAGAGGGATAC-3′ 3:4493967-4493986:- wh221@cinj.rutgers.edu, fengzh@cinj.rutgers.edu Accepted 2021-APR-13 AT 11:24 BST AT 11:24 BST by MGT Cell death and differentiation 2015 Only protein-protein interactions imex curation imex curation Pontin, a new mutant p53-binding protein, promotes gain-of-function of mutant p53. Pontin, a new mutant p53-binding protein, promotes gain-of-function of mutant p53. Cell death and differentiation 2015 imex curation Zhao Y., Zhang C., Yue X., Li X., Liu J., Yu H., Belyi VA., Yang Q., Feng Z., Hu W. wh221@cinj.rutgers.edu, fengzh@cinj.rutgers.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Zhao Y., Zhang C., Yue X., Li X., Liu J., Yu H., Belyi VA., Yang Q., Feng Z., Hu W. wh221@cinj.rutgers.edu, fengzh@cinj.rutgers.edu Accepted 2021-APR-13 AT 11:24 BST AT 11:24 BST by MGT Cell death and differentiation 2015 Only protein-protein interactions imex curation imex curation Pontin, a new mutant p53-binding protein, promotes gain-of-function of mutant p53. Pontin, a new mutant p53-binding protein, promotes gain-of-function of mutant p53. Cell death and differentiation 2015 imex curation Zhao Y., Zhang C., Yue X., Li X., Liu J., Yu H., Belyi VA., Yang Q., Feng Z., Hu W. wh221@cinj.rutgers.edu, fengzh@cinj.rutgers.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Zhao Y., Zhang C., Yue X., Li X., Liu J., Yu H., Belyi VA., Yang Q., Feng Z., Hu W. H1299 P53 KO cells were used in this experiment. wh221@cinj.rutgers.edu, fengzh@cinj.rutgers.edu Accepted 2021-APR-13 AT 11:24 BST AT 11:24 BST by MGT Cell death and differentiation 2015 Only protein-protein interactions imex curation imex curation ruvb1_human RuvB-like 1 RUVBL1 NMP238 TIP49 TIP49A 49 kDa TATA box-binding protein-interacting protein TIP49a Pontin 52 Nuclear matrix protein 238 54 kDa erythrocyte cytosolic protein INO80H TIP60-associated protein 54-alpha INO80 complex subunit H protein protein human Homo sapiens Human MKIEEVKSTTKTQRIASHSHVKGLGLDESGLAKQAASGLVGQENAREACGVIVELIKSKKMAGRAVLLAGPPGTGKTALALAIAQELGSKVPFCPMVGSEVYSTEIKKTEVLMENFRRAIGLRIKETKEVYEGEVTELTPCETENPMGGYGKTISHVIIGLKTAKGTKQLKLDPSIFESLQKERVEAGDVIYIEANSGAVKRQGRCDTYATEFDLEAEEYVPLPKGDVHKKKEIIQDVTLHDLDVANARPQGGQDILSMMGQLMKPKKTEITDKLRGEINKVVNKYIDQGIAELVPGVLFVDEVHMLDIECFTYLHRALESSIAPIVIFASNRGNCVIRGTEDITSPHGIPLDLLDRVMIIRTMLYTPQEMKQIIKIRAQTEGINISEEALNHLGEIGTKTTLRYSVQLLTPANLLAKINGKDSIEKEHVEEISELFYDAKSSAKILADQQDKYMK DIP protein Q9Y265 original sequence version: 82 DIP protein Q9Y265 original sequence version: 110 6095ADE692B1482B p53_mouse Cellular tumor antigen p53 Tp53 P53 Trp53 Tumor suppressor p53 protein protein mouse Mus musculus Mouse MTAMEESQSDISLELPLSQETFSGLWKLLPPEDILPSPHCMDDLLLPQDVEEFFEGPSEALRVSGAPAAQDPVTETPGPVAPAPATPWPLSSFVPSQKTYQGNYGFHLGFLQSGTAKSVMCTYSPPLNKLFCQLAKTCPVQLWVSATPPAGSRVRAMAIYKKSQHMTEVVRRCPHHERCSDGDGLAPPQHLIRVEGNLYPEYLEDRQTFRHSVVVPYEPPEAGSEYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEVLCPELPPGSAKRALPTCTSASPPQKKKPLDGEYFTLKIRGRKRFEMFRELNEALELKDAHATEESGDSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSD DIP protein P02340 original sequence version: 117 DIP protein P02340 original sequence version: 165 DIP protein P02340 original sequence version: 187 DIP protein P02340 original sequence version: 201 DIP protein P02340 original sequence version: 111 DIP protein P02340 original sequence version: 141 DIP protein P02340 original sequence version: 180 DIP protein P02340 original sequence version: 163 DIP protein P02340 original sequence version: 152 ED5A607378D921D7 stmn1_human_gene Stathmin STMN1 C1orf215 LAP18 OP18 gene gene human Homo sapiens Human itpr1_human_gene ITPR1 gene gene human Homo sapiens Human ripk1_human_gene Receptor-interacting serine/threonine-protein kinase 1 RIPK1 RIP RIP1 gene gene human Homo sapiens Human ruvb1_mouse RuvB-like 1 Ruvbl1 Tip49 Tip49a 49 kDa TATA box-binding protein-interacting protein TIP49a Pontin 52 DNA helicase p50 protein protein mouse Mus musculus Mouse MKIEEVKSTTKTQRIASHSHVKGLGLDESGLAKQAASGLVGQENAREACGVIVELIKSKKMAGRAVLLAGPPGTGKTALALAIAQELGSKVPFCPMVGSEVYSTEIKKTEVLMENFRRAIGLRIKETKEVYEGEVTELTPCETENPMGGYGKTISHVIIGLKTAKGTKQLKLDPSIFESLQKERVEAGDVIYIEANSGAVKRQGRCDTYATEFDLEAEEYVPLPKGDVHKKKEIIQDVTLHDLDVANARPQGGQDILSMMGQLMKPKKTEITDKLRGEINKVVNKYIDQGVAELVPGVLFVDEVHMLDIECFTYLHRALESSIAPIVIFASNRGNCVIRGTEDITSPHGIPLDLLDRVMIIRTMLYTPQEMKQIIKIRAQTEGINISEEALNHLGEIGTKTTLRYSVQLLTPANLLAKINGKDSIEKEHVEEISELFYDAKSSAKILADQQDKYMK 9AE73EFFBA194BA1 p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 ruvbl1-tp53-1 1 14 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed P4 fragment required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain P04637:p.Arg248Gln mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain R Q P04637:p.Arg273His mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain R H P04637:p.Arg175His mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain R H P3 fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1a, b, d ruvbl1-tp53-2 7 14 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed P04637:p.Arg175His mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain R H 8 anti tag western anti tag western blot anti tag western anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1a tp53-ruvbl1-1 2 14 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H 8 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association 1c ruvbl1-tp53-3 2 14 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed P04637:p.Arg175His mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain R H 8 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association 1c ruvbl1-tp53-4 3 13 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 9 unspecified role unspecified role bait bait engineered engineered P02340:p.Arg172His mutation mutation certain certain sequence position certain certain certain sequence position certain R H physical association physical association 1e This interaction is only found in tumour tissue, not in healthy thymus. ruvbl1-tp53-5 1 14 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H 8 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9Y265:p.Asp302Asn mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain D N physical association physical association 4a, 3a, b ruvbl1-tp53-6 4 14 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed P04637:p.Arg175His mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain R H 8 anti tag western anti tag western blot anti tag western immunocytochemistry immunocytochemistry unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association 5a tp53-ruvbl1-2 6 14 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed P04637:p.Arg175His mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain R H 8 anti tag western anti tag western blot anti tag western immunocytochemistry immunocytochemistry unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association 5a stmn1-stmn1-1 5 8 unspecified role unspecified role ancillary ancillary physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role prey prey SREBP-binding site sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 1:25906409-25906736 Primers used: F: 5′-GAGAATGGGGAGCTGGTTCG-3′ (1:25906717-25906736:-) R: 5′-GACCACACTCTGAGCACCAA-3′ (1:25906409-25906428:+)(1:25906409-25906428:+) 14 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed engineered engineered P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H physical association physical association 5e tp53-ripk1-1 5 8 unspecified role unspecified role ancillary ancillary physiological level physiological level endogenous level endogenous endogenous endogenous level 12 unspecified role unspecified role prey prey NF-κB-binding site sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6:3064454-3064791 Primers used: F: 5′-ATCCGAGCACTCTTACCCATTCTA-3′ (6:3064454-3064477:+) R: 5′-GTCACCTCTTCGCAAACTCCTG-3′ (6:3064770-3064791:-) 14 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed engineered engineered P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H physical association physical association 5e tp53-itpr1-1 5 8 unspecified role unspecified role ancillary ancillary physiological level physiological level endogenous level endogenous endogenous endogenous level 11 unspecified role unspecified role prey prey NF-κB-binding site sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3:4493661-4493986 Primers used: F: 5′-CAGAAGTTTTTGCCCCGCTC-3′ (3:4493661-4493680:+) R: 5′-GGAGCGCAAGAGAGGGATAC-3′ (3:4493967-4493986:-) 14 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed engineered engineered P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H physical association physical association 5e