UniProt UniProt http://www.uniprot.org The terminal enzymes of cholesterol synthesis, DHCR24 and DHCR7, interact physically and functionally. The terminal enzymes of cholesterol synthesis, DHCR24 and DHCR7, interact physically and functionally. Journal of lipid research 2015 imex curation Luu W., Hart-Smith G., Sharpe LJ., Brown AJ. aj.brown@unsw.edu.au Only protein-protein interactions imex curation crigr-cho7 crigr cho7 cho7 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Luu W., Hart-Smith G., Sharpe LJ., Brown AJ. Not sure where the His comes from - does not appear to be part of the vector so could just be the proteins are his-rich. I've just made a vague exp-mod and gone with the text. Proteins were further identified by anti-His Abs and by molecular weight. aj.brown@unsw.edu.au Accepted 2021-JUN-28 AT 08:10 BST AT 08:10 BST by KALPANAP Journal of lipid research 2015 Only protein-protein interactions imex curation imex curation The terminal enzymes of cholesterol synthesis, DHCR24 and DHCR7, interact physically and functionally. The terminal enzymes of cholesterol synthesis, DHCR24 and DHCR7, interact physically and functionally. Journal of lipid research 2015 imex curation Luu W., Hart-Smith G., Sharpe LJ., Brown AJ. aj.brown@unsw.edu.au Only protein-protein interactions imex curation crigr-cho7 crigr cho7 cho7 anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Luu W., Hart-Smith G., Sharpe LJ., Brown AJ. Not sure where the his comes from - does not appear to be part of the vector so could just be the proteins are his-rich, so I've made a vague exp-mod and gone with the text. Proteins were further identified by anti-His Abs and by molecular weight. aj.brown@unsw.edu.au Accepted 2021-JUN-28 AT 08:10 BST AT 08:10 BST by KALPANAP Journal of lipid research 2015 Only protein-protein interactions imex curation imex curation a0a3l7i5z2_crigr CgPICR_011489 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MNLGSDPVVIVSAARTAIGGYPRRSPIRYPAGAQSSFNGALSTVPVHDLGTTVIKEVLQRAKVAPEEVSEVIFGHVLAAGCGQNPTRQASVGAGIPYPVPAWSCQMICGSGLKAVCLAAQSIAIGDSSIVVAGGMENMSKAPHLAHLRAGVKMGEMPLADSILCDGLTDAFHNYHMGITAENVAKKWQVSREAQDKVAVVSQNRAEHAQKAGHFDKEIVPVLVSSRKGPTEVKIDEFPRHGSNMEAVGKLKPYFLSDGTGTVTTANATGMNDGAAAVVLMKTTEAKRRMLKPLARIVSWSQAGVEPSIMGVGPIPAIKQAVAKAGWTLEDVDLFEINEAYAALSVAITKELGLNPEKVNIDGGAIALGHPLGASGCRILVTLLHSLERTGGTRGVAALCIGGGMGIAMCVQRG A94F1C1CD3B747DC a0a3l7ipt3_crigr Squalene monooxygenase CgPICR_011593 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MWTFLGIATFTYFYKKCGDVTLANKELLLCVLVFLSLGLVLSYRCRHRNAGLLGRQQSAAGGSQFAALSDILSALPLIGFLWAKSPPESEKKEEPESSKCRKETSVSETTLTGAATSVTASSLNDPEVIIVGSGVLGSALATVLSRDGRKVTVIERDLKEPDRIVGELLQPGGYRVLKELGLGDTVEGFNAHLIHGYIVHDYESRSEVQIPYPVSENNQVQNGVAFHHGNVRVIEGVVLQLLEDGDTVTGVQYKDKETGDTKELHAPLTVVADGLFSKFRKNLISSKVSVSSHFVGFLMKNAPQFKANFAELVLLNPSPVLIYQISASETRVLVDIRGEMPRNLREYMAEQIYPQIPDHLKEAFLEASQSARLRTMPASFLPPSPVNKRGVLLLGDAYNLRHPLTGGGMTVALKDVKLWRKLLKGIPDLYDDAAVFQAKKSFYWSRKRTHSFVVNVLAQALYELFSATDDSLHQLRKACFLYFKLGGECVTGPVGLLSVLSPHPLVLIRHFFAVAIYATYFCFKSEPWATKPRALFSSGAVLYKACSIIFPLIYSEMKYLVH F442E0AB46B2F53C dhc24_human Delta(24)-sterol reductase DHCR24 KIAA0018 24-dehydrocholesterol reductase Diminuto/dwarf1 homolog Seladin-1 3-beta-hydroxysterol Delta-24-reductase protein protein human Homo sapiens Human MEPAVSLAVCALLFLLWVRLKGLEFVLIHQRWVFVCLFLLPLSLIFDIYYYVRAWVVFKLSSAPRLHEQRVRDIQKQVREWKEQGSKTFMCTGRPGWLTVSLRVGKYKKTHKNIMINLMDILEVDTKKQIVRVEPLVTMGQVTALLTSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELVLADGSFVRCTPSENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGLEAICAKFTHESQRQENHFVEGLLYSLDEAVIMTGVMTDEAEPSKLNSIGNYYKPWFFKHVENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMVPPKISLLKLTQGETLRKLYEQHHVVQDMLVPMKCLQQALHTFQNDIHVYPIWLCPFILPSQPGLVHPKGNEAELYIDIGAYGEPRVKHFEARSCMRQLEKFVRSVHGFQMLYADCYMNREEFWEMFDGSLYHKLREKLGCQDAFPEVYDKICKAARH F9A769446FE19E59 a0a3l7hwl4_crigr CgPICR_009611 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster DLMASKFQHNTPKTKNPNGKAESQGQWGRAWEVDWFSLASVIFLLLFAPFIVYYFIMACDQYSCSLTTPIVDIATGRASLADIWAKTPPVTAKAVQLYTLWVSFQVVLYSWLPDFCHRFLPGYVGGVQEGAVTPAGVVNKYEVNGLQAWLITHLLWFLNAYLLSWFSPTIIFDNWIPLLWCANILGYAWFDFKLFFNGRPGIVAWTLINLSFAAKQQELYGHVTNSMILVNVLQAIYVLDFFWNETWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYHPVQLSTPNAVGILLLGLVGYYIFRMANHQKDLFRRTDGRCLIWGKKPKAIECSYTSADGQKHHSKLLVSGFWGVARHLNYTGDLMGSLAYCLACGGGHLLPYFYIIYMTILLTHRCLRDEHRCANKYGRDWERYTATVPYRLLPGIF 67F0378A777E43B9 dhcr7_human 7-dehydrocholesterol reductase Sterol Delta(7)-reductase DHCR7 D7SR Delta7-sterol reductase Sterol reductase SR-2 protein protein human Homo sapiens Human MAAKSQPNIPKAKSLDGVTNDRTASQGQWGRAWEVDWFSLASVIFLLLFAPFIVYYFIMACDQYSCALTGPVVDIVTGHARLSDIWAKTPPITRKAAQLYTLWVTFQVLLYTSLPDFCHKFLPGYVGGIQEGAVTPAGVVNKYQINGLQAWLLTHLLWFANAHLLSWFSPTIIFDNWIPLLWCANILGYAVSTFAMVKGYFFPTSARDCKFTGNFFYNYMMGIEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSFAAKQRELHSHVTNAMVLVNVLQAIYVIDFFWNETWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYHPVQLSTPHAVGVLLLGLVGYYIFRVANHQKDLFRRTDGRCLIWGRKPKVIECSYTSADGQRHHSKLLVSGFWGVARHFNYVGDLMGSLAYCLACGGGHLLPYFYIIYMAILLTHRCLRDEHRCASKYGRDWERYTAAVPYRLLPGIF 7D726443834C4EEB hmdh_crigr 3-hydroxy-3-methylglutaryl-coenzyme A reductase HMGCR protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MLSRLFRMHGLFVASHPWEVIVGTVTLTICMMSMNMFTGNNKICGWNYECPKFEEDVLSSDIIILTITRCIAILYIYFQFQNLRQLGSKYILGIAGLFTIFSSFVFSTVVIHFLDKELTGLNEALPFFLLLIDLSRASALAKFALSSNSQDEVRENIARGMAILGPTFTLDALVECLVIGVGTMSGVRQLEIMCCFGCMSVLANYFVFMTFFPACVSLVLELSRESREGRPIWQLSHFARVLEEEENKPNPVTQRVKMIMSLGLVLVHAHSRWIADPSPQNSTTEHSKVSLGLDEDVSKRIEPSVSLWQFYLSKMISMDIEQVVTLSLAFLLAVKYIFFEQAETESTLSLKNPITSPVVTPKKAPDNCCRREPLLVRRSEKLSSVEEEPGVSQDRKVEVIKPLVVETESASRATFVLGASGTSPPVAARTQELEIELPSEPRPNEECLQILESAEKGAKFLSDAEIIQLVNAKHIPAYKLETLMETHERGVSIRRQLLSTKLPEPSSLQYLPYRDYNYSLVMGACCENVIGYMPIPVGVAGPLCLDGKEYQVPMATTEGCLVASTNRGCRAIGLGGGASSRVLADGMTRGPVVRLPRACDSAEVKAWLETPEGFAVIKDAFDSTSRFARLQKLHVTMAGRNLYIRFQSKTGDAMGMNMISKGTEKALLKLQEFFPEMQILAVSGNYCTDKKPAAINWIEGRGKTVVCEAVIPAKVVREVLKTTTEAMIDVNINKNLVGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITLMEASGPTNEDLYISCTMPSIEIGTVGGGTNLLPQQACLQMLGVQGACKDNPGENARQLARIVCGTVMAGELSLMAALAAGHLVRSHMVHNRSKINLQDLQGTCTKKSA 4331E53ADA250E6A g3gra0_crigr 3-beta-hydroxysterol Delta (14)-reductase C-14 sterol reductase Delta(14)-sterol reductase LBR Integral nuclear envelope inner membrane protein Lamin-B receptor Sterol C14-reductase I79_000043 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MPSRKFAEGEVVRGRWPGSSLYYEVEILSYDNKSQLYTVKYKDGTELELKESDVKPLKSFKQRKGGSTSSSPSRRRSSRSRSRSRSPGRAPKGSRRSASASHQADIKEKETRREILQVKLTPLVLKPFGNSINVYNGEPEHTEKNDISRKNKQEKVILSPEGSFIATQYSLRPRREEVTVKEIESKEQKRLPKVPAPLETFQVTTPQRKSLEFGGVPGASLIMLGLPACLFLLLLQCRQKDPSLLSFPPPLPALGELWEARVCGIYLLWFFIQALFYLLPIGKVVEGTPLVDGRRLKYRLNGFNAFVLTSAIVGTSLFWGIELCYVYTHFLQFALAATVFSVVLSVYLYARSLKAPRDELSPASSGNAVYDFFIGRELNPRIGVFDLKYFCELRPGLIGWVVVNLVMLLAEMKVQERGTPSLAMVLVNSFQLLYVVDALWNEEALLTTMDIIHDGFGFMLAFGDLVWVPFTYSLQAFYLVSHPHDLSWPLASVIIALKLCGYVIFRCANSQKNAFRKNPTDPKLAHLKTIHTSTGKSLLVSGWWGFVRHPNYLGDLIMALAWSLPCGFNHLLPYFYVIYFTALLVHREARDEHQCKRKYGLAWEKYCQRVPYRILPYVY 0AE1BB530DE5636A a0a3l7h248_crigr CgPICR_022031 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster KLILTSSASVVFEGVDIKNGTEDLPYAMKPIDYYTETKILQEREVLDANNPDKNFLTTAIRPHGIFGPRDPQLVPILIDAARKGKMKFMIGNGKNLVDFTFVENVVHGHILAAEHLSQDAALGGKAFHITNDEPIPFWTFLSRMLTGLNYEAPKYHIPYWVAYYLAFLFSLLVMVVSPLIQIQPTFTPMRVALAGTFHYYSCEKAKKLFGYRPLVTMDDAVERTVQSFHHLRKGK 0745F448E137D8A1 g3h0l7_crigr Squalene synthase CgPICR_007530 I79_003679 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MEFVKCLGHPEEFYNLLRFRMGGRRNFITKMDQDSLSSSLKTCYKYLNQTSRSFAAVIQALDGEIRHAVCVFYLVLRALDTVEDDMSISVEKKIPMLRNFHTFLYEPEWRFTESTQKDRQVLEDFPTISLEFRNLAEKYQTVIADICQKMGCGMAEFVDKHVTSKQDWDKYCHYVAGLVGIGLSRLFSTSEFEDPLVGEDTECANSMGLFLQKTNIIRDYLEDQQEGRAFWPQEVWGRYVKKLEDFAKPENIDVAVQCLNELITNTLHHIPHVITYLSRLRNQSVFNFCAIPQVMAIATLAACYNNQQVFKGVVKIRKGQAVTLMMDATNMPAVKAIIHQYIEEIHHRIPSSDPSSSKTKEVISAIRTQSLPNCQLISRSHYSPIYLSFVMLLAALSWQYLSTLSQVTEDYVQTGEH 6633281E4D9551C8 dhcr7-dhcr24-1 1 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 2 dhcr24-acat2-1 2 10 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level 9 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level 4 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level 3 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level 11 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level association association Table 1