MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Ubiquitin C-terminal hydrolase L1 (UCH-L1) acts as a novel potentiator of cyclin-dependent kinases to enhance cell proliferation independently of its hydrolase activity. Ubiquitin C-terminal hydrolase L1 (UCH-L1) acts as a novel potentiator of cyclin-dependent kinases to enhance cell proliferation independently of its hydrolase activity. The Journal of biological chemistry 2013 imex curation Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. I did not add the in vitro kinase assay in fig.2 because I was not able to trace the species used. SOD1 species not specified. kabuta@ncnp.go.jp Only protein-protein interactions imex curation mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells antibody array antibody array sandwich immunoassay antigen capture assay antibody detection identification by antibody antibody detection Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K. Parkinsons - Interactions investigated in the context of Parkinsons disease I did not add the in vitro kinase assay in fig.2 because I was not able to trace the species used. SOD1 species not specified. kabuta@ncnp.go.jp Accepted 2022-JAN-14 AT 09:38 GMT AT 09:38 GMT by MGT The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation Ubiquitin C-terminal hydrolase L1 (UCH-L1) acts as a novel potentiator of cyclin-dependent kinases to enhance cell proliferation independently of its hydrolase activity. Ubiquitin C-terminal hydrolase L1 (UCH-L1) acts as a novel potentiator of cyclin-dependent kinases to enhance cell proliferation independently of its hydrolase activity. The Journal of biological chemistry 2013 imex curation Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. I did not add the in vitro kinase assay in fig.2 because I was not able to trace the species used. SOD1 species not specified. kabuta@ncnp.go.jp Only protein-protein interactions imex curation mouse-neuro_2_a Mus musculus albino neuroblastoma cell line neuro 2 a (N2a) neuro_2_a Mouse Albino neuroblastoma neuro 2 a cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K. Parkinsons - Interactions investigated in the context of Parkinsons disease I did not add the in vitro kinase assay in fig.2 because I was not able to trace the species used. SOD1 species not specified. kabuta@ncnp.go.jp Accepted 2022-JAN-14 AT 09:38 GMT AT 09:38 GMT by MGT The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation Ubiquitin C-terminal hydrolase L1 (UCH-L1) acts as a novel potentiator of cyclin-dependent kinases to enhance cell proliferation independently of its hydrolase activity. Ubiquitin C-terminal hydrolase L1 (UCH-L1) acts as a novel potentiator of cyclin-dependent kinases to enhance cell proliferation independently of its hydrolase activity. The Journal of biological chemistry 2013 imex curation Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. I did not add the in vitro kinase assay in fig.2 because I was not able to trace the species used. SOD1 species not specified. kabuta@ncnp.go.jp Only protein-protein interactions imex curation in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K. Parkinsons - Interactions investigated in the context of Parkinsons disease Results from figure 2C have not been added as the species origin of p27 cannot be traced. I did not add the in vitro kinase assay in fig.2 because I was not able to trace the species used. SOD1 species not specified. kabuta@ncnp.go.jp Accepted 2022-JAN-14 AT 09:38 GMT AT 09:38 GMT by MGT The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation Ubiquitin C-terminal hydrolase L1 (UCH-L1) acts as a novel potentiator of cyclin-dependent kinases to enhance cell proliferation independently of its hydrolase activity. Ubiquitin C-terminal hydrolase L1 (UCH-L1) acts as a novel potentiator of cyclin-dependent kinases to enhance cell proliferation independently of its hydrolase activity. The Journal of biological chemistry 2013 imex curation Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. I did not add the in vitro kinase assay in fig.2 because I was not able to trace the species used. SOD1 species not specified. kabuta@ncnp.go.jp Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K. Parkinsons - Interactions investigated in the context of Parkinsons disease I did not add the in vitro kinase assay in fig.2 because I was not able to trace the species used. SOD1 species not specified. kabuta@ncnp.go.jp Accepted 2022-JAN-14 AT 09:38 GMT AT 09:38 GMT by MGT The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation Ubiquitin C-terminal hydrolase L1 (UCH-L1) acts as a novel potentiator of cyclin-dependent kinases to enhance cell proliferation independently of its hydrolase activity. Ubiquitin C-terminal hydrolase L1 (UCH-L1) acts as a novel potentiator of cyclin-dependent kinases to enhance cell proliferation independently of its hydrolase activity. The Journal of biological chemistry 2013 imex curation Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. I did not add the in vitro kinase assay in fig.2 because I was not able to trace the species used. SOD1 species not specified. kabuta@ncnp.go.jp Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K. Parkinsons - Interactions investigated in the context of Parkinsons disease I did not add the in vitro kinase assay in fig.2 because I was not able to trace the species used. SOD1 species not specified. kabuta@ncnp.go.jp Accepted 2022-JAN-14 AT 09:38 GMT AT 09:38 GMT by MGT The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation Ubiquitin C-terminal hydrolase L1 (UCH-L1) acts as a novel potentiator of cyclin-dependent kinases to enhance cell proliferation independently of its hydrolase activity. Ubiquitin C-terminal hydrolase L1 (UCH-L1) acts as a novel potentiator of cyclin-dependent kinases to enhance cell proliferation independently of its hydrolase activity. The Journal of biological chemistry 2013 imex curation Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. I did not add the in vitro kinase assay in fig.2 because I was not able to trace the species used. SOD1 species not specified. kabuta@ncnp.go.jp Only protein-protein interactions imex curation chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K., Kabuta T., Mitsui T., Takahashi M., Fujiwara Y., Kabuta C., Konya C., Tsuchiya Y., Hatanaka Y., Uchida K., Hohjoh H., Wada K. Parkinsons - Interactions investigated in the context of Parkinsons disease I did not add the in vitro kinase assay in fig.2 because I was not able to trace the species used. SOD1 species not specified. kabuta@ncnp.go.jp Accepted 2022-JAN-14 AT 09:38 GMT AT 09:38 GMT by MGT The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation Mock publication and experiment for abstract interactions that are not interaction evidences. Mock publication and experiment for abstract interactions that are not interaction evidences. unspecified method cdk4_human Cyclin-dependent kinase 4 CDK4 Cell division protein kinase 4 PSK-J3 protein protein human Homo sapiens Human MATSRYEPVAEIGVGAYGTVYKARDPHSGHFVALKSVRVPNGGGGGGGLPISTVREVALLRRLEAFEHPNVVRLMDVCATSRTDREIKVTLVFEHVDQDLRTYLDKAPPPGLPAETIKDLMRQFLRGLDFLHANCIVHRDLKPENILVTSGGTVKLADFGLARIYSYQMALTPVVVTLWYRAPEVLLQSTYATPVDMWSVGCIFAEMFRRKPLFCGNSEADQLGKIFDLIGLPPEDDWPRDVSLPRGAFPPRGPRPVQSVVPEMEESGAQLLLEMLTFNPHKRISAFRALQHSYLHKDEGNPE DIP protein P11802 original sequence version: 177 DIP protein P11802 original sequence version: 135 DIP protein P11802 original sequence version: 136 DIP protein P11802 original sequence version: 200 DIP protein P11802 original sequence version: 86 0916A0C07403A33A cdk5_human Cyclin-dependent-like kinase 5 CDK5 Cell division protein kinase 5 Tau protein kinase II catalytic subunit Serine/threonine-protein kinase PSSALRE CDKN5 protein protein human Homo sapiens Human MQKYEKLEKIGEGTYGTVFKAKNRETHEIVALKRVRLDDDDEGVPSSALREICLLKELKHKNIVRLHDVLHSDKKLTLVFEFCDQDLKKYFDSCNGDLDPEIVKSFLFQLLKGLGFCHSRNVLHRDLKPQNLLINRNGELKLADFGLARAFGIPVRCYSAEVVTLWYRPPDVLFGAKLYSTSIDMWSAGCIFAELANAGRPLFPGNDVDDQLKRIFRLLGTPTEEQWPSMTKLPDYKPYPMYPATTSLVNVVPKLNATGRDLLQNLLKCNPVQRISAEEALQHPYFSDFCPP DIP protein Q00535 original sequence version: 129 DIP protein Q00535 original sequence version: 140 54D10495F017D527 rb_human Retinoblastoma-associated protein pRb pp110 p105-Rb RB1 p110-RB1 protein protein human Homo sapiens Human MPPKTPRKTAATAAAAAAEPPAPPPPPPPEEDPEQDSGPEDLPLVRLEFEETEEPDFTALCQKLKIPDHVRERAWLTWEKVSSVDGVLGGYIQKKKELWGICIFIAAVDLDEMSFTFTELQKNIEISVHKFFNLLKEIDTSTKVDNAMSRLLKKYDVLFALFSKLERTCELIYLTQPSSSISTEINSALVLKVSWITFLLAKGEVLQMEDDLVISFQLMLCVLDYFIKLSPPMLLKEPYKTAVIPINGSPRTPRRGQNRSARIAKQLENDTRIIEVLCKEHECNIDEVKNVYFKNFIPFMNSLGLVTSNGLPEVENLSKRYEEIYLKNKDLDARLFLDHDKTLQTDSIDSFETQRTPRKSNLDEEVNVIPPHTPVRTVMNTIQQLMMILNSASDQPSENLISYFNNCTVNPKESILKRVKDIGYIFKEKFAKAVGQGCVEIGSQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSTSQNLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKAEGNLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDREGPTDHLESACPLNLPLQNNHTAADMYLSPVRSPKKKGSTTRVNSTANAETQATSAFQTQKPLKSTSLSLFYKKVYRLAYLRLNTLCERLLSEHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAVQETFKRVLIKEEEYDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFPSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGSNPPKPLKKLRFDIEGSDEADGSKHLPGESKFQQKLAEMTSTRTRMQKQKMNDSMDTSNKEEK DIP protein P06400 original sequence version: 102 DIP protein P06400 original sequence version: 195 DIP protein P06400 original sequence version: 204 DIP protein P06400 original sequence version: 126 DIP protein P06400 original sequence version: 151 DIP protein P06400 original sequence version: 153 DIP protein P06400 original sequence version: 181 DIP protein P06400 original sequence version: 184 DIP protein P06400 original sequence version: 213 DIP protein P06400 original sequence version: 208 DIP protein P06400 original sequence version: 174 DIP protein P06400 original sequence version: 212 DIP protein P06400 original sequence version: 179 DIP protein P06400 original sequence version: 146 C8E746111E19CC32 ccnb1_human G2/mitotic-specific cyclin-B1 CCNB1 CCNB protein protein human Homo sapiens Human MALRVTRNSKINAENKAKINMAGAKRVPTAPAATSKPGLRPRTALGDIGNKVSEQLQAKMPMKKEAKPSATGKVIDKKLPKPLEKVPMLVPVPVSEPVPEPEPEPEPEPVKEEKLSPEPILVDTASPSPMETSGCAPAEEDLCQAFSDVILAVNDVDAEDGADPNLCSEYVKDIYAYLRQLEEEQAVRPKYLLGREVTGNMRAILIDWLVQVQMKFRLLQETMYMTVSIIDRFMQNNCVPKKMLQLVGVTAMFIASKYEEMYPPEIGDFAFVTDNTYTKHQIRQMEMKILRALNFGLGRPLPLHFLRRASKIGEVDVEQHTLAKYLMELTMLDYDMVHFPPSQIAAGAFCLALKILDNGEWTPTLQHYLSYTEESLLPVMQHLAKNVVMVNQGLTKHMTVKNKYATSKHAKISTLPQLNSALVQDLAKAVAKV DIP protein P14635 original sequence version: 146 DIP protein P14635 original sequence version: 108 DIP protein P14635 original sequence version: 145 DIP protein P14635 original sequence version: 139 E2C4767AE8A11EC0 cdk4_chlae_protein cdk4_chlae_protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey Simlar to UniProt P11802 sodc_human Superoxide dismutase [Cu-Zn] Superoxide dismutase 1 SOD1 protein protein human Homo sapiens Human MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ DIP protein P00441 original sequence version: 194 DIP protein P00441 original sequence version: 189 DIP protein P00441 original sequence version: 186 DIP protein P00441 original sequence version: 147 DIP protein P00441 original sequence version: 148 DIP protein P00441 original sequence version: 145 DIP protein P00441 original sequence version: 169 25CA38DA8D564483 cdk1_human Cyclin-dependent kinase 1 p34 protein kinase Cell division protein kinase 1 CDK1 CDC2 Cell division control protein 2 homolog CDC28A CDKN1 P34CDC2 protein protein human Homo sapiens Human MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRHPNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM DIP protein P06493 original sequence version: 185 DIP protein P06493 original sequence version: 162 DIP protein P06493 original sequence version: 184 DIP protein P06493 original sequence version: 97 DIP protein P06493 original sequence version: 192 942D79448EFE490A cdk5_chlae_protein cdk5_chlae_protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey Simlar to UniProt Q00535 uchl1_mouse Ubiquitin carboxyl-terminal hydrolase isozyme L1 Uchl1 Ubiquitin thioesterase L1 Neuron cytoplasmic protein 9.5 PGP 9.5 protein protein mouse Mus musculus Mouse MQLKPMEINPEMLNKVLAKLGVAGQWRFADVLGLEEETLGSVPSPACALLLLFPLTAQHENFRKKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLEFEDGSVLKQFLSETEKLSPEDRAKCFEKNEAIQAAHDSVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDSLLQDAAKVCREFTEREQGEVRFSAVALCKAA F1402BF7B0C077EA cdk2_mouse Cyclin-dependent kinase 2 Cdk2 Cell division protein kinase 2 Cdkn2 protein protein mouse Mus musculus Mouse MENFQKVEKIGEGTYGVVYKAKNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINAEGSIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMHLVCTQHHAKCCGEHRRNGRHSLCPLCSYLEVAASQGGGMTAVSAPHPVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL DIP protein P97377 original sequence version: 140 D806BC2F150AEDFC uchl1_human Ubiquitin carboxyl-terminal hydrolase isozyme L1 Ubiquitin thioesterase L1 Neuron cytoplasmic protein 9.5 PGP 9.5 UCHL1 protein protein human Homo sapiens Human MQLKPMEINPEMLNKVLSRLGVAGQWRFVDVLGLEEESLGSVPAPACALLLLFPLTAQHENFRKKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQAAHDAVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLKDAAKVCREFTEREQGEVRFSAVALCKAA DIP protein P09936 original sequence version: 135 C9E972AC4DA5DA8A cdn1b_human Cyclin-dependent kinase inhibitor 1B Cyclin-dependent kinase inhibitor p27 p27Kip1 CDKN1B KIP1 protein protein human Homo sapiens Human MSNVRVSNGSPSLERMDARQAEHPKPSACRNLFGPVDHEELTRDLEKHCRDMEEASQRKWNFDFQNHKPLEGKYEWQEVEKGSLPEFYYRPPRPPKGACKVPAQESQDVSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATDDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT DIP protein P46527 original sequence version: 120 DIP protein P46527 original sequence version: 115 DIP protein P46527 original sequence version: 154 DIP protein P46527 original sequence version: 168 DIP protein P46527 original sequence version: 164 DIP protein P46527 original sequence version: 118 1118D58901CDF3FC cdn1a_human Cyclin-dependent kinase inhibitor 1 CDKN1A CAP20 CDKN1 CIP1 MDA6 PIC1 SDI1 WAF1 p21 CDK-interacting protein 1 Melanoma differentiation-associated protein 6 protein protein human Homo sapiens Human MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEARERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQGTAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRKP DIP protein P38936 original sequence version: 120 DIP protein P38936 original sequence version: 165 DIP protein P38936 original sequence version: 160 98D1E7C519ADFCA9 cdk1_chlae_protein cdk1_chlae_protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey Simlar to UniProt P06493 cdk6_chlae_protein cdk6_chlae_protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey Simlar to UniProt Q00534 cdk2_human Cyclin-dependent kinase 2 CDK2 p33 protein kinase Cell division protein kinase 2 CDKN2 protein protein human Homo sapiens Human MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL DIP protein P24941 original sequence version: 142 DIP protein P24941 original sequence version: 118 DIP protein P24941 original sequence version: 143 DIP protein P24941 original sequence version: 144 DIP protein P24941 original sequence version: 152 DIP protein P24941 original sequence version: 182 DIP protein P24941-1 original sequence version: 204 DIP protein P24941 original sequence version: 90 DIP protein P24941 original sequence version: 190 DIP protein P24941 original sequence version: 168 F90A0F4E70910B51 cdk4_mouse Cyclin-dependent kinase 4 Cell division protein kinase 4 PSK-J3 CRK3 Cdk4 Crk3 protein protein mouse Mus musculus Mouse MAATRYEPVAEIGVGAYGTVYKARDPHSGHFVALKSVRVPNGGAAGGGLPVSTVREVALLRRLEAFEHPNVVRLMDVCATSRTDRDIKVTLVFEHIDQDLRTYLDKAPPPGLPVETIKDLMRQFLSGLDFLHANCIVHRDLKPENILVTSNGTVKLADFGLARIYSYQMALTPVVVTLWYRAPEVLLQSTYATPVDMWSVGCIFAEMFRRKPLFCGNSEADQLGKIFDLIGLPPEDDWPREVSLPRGAFAPRGPRPVQSVVPEMEESGAQLLLEMLTFNPHKRISAFRALQHSYLHKEESDAE DIP protein P30285 original sequence version: 145 DIP protein P30285 original sequence version: 129 DIP protein P30285 original sequence version: 82 DIP protein P30285 original sequence version: 102 CB4F42A8AA13634A cdk6_human Cyclin-dependent kinase 6 CDK6 Serine/threonine-protein kinase PLSTIRE Cell division protein kinase 6 CDKN6 protein protein human Homo sapiens Human MEKDGLCRADQQYECVAEIGEGAYGKVFKARDLKNGGRFVALKRVRVQTGEEGMPLSTIREVAVLRHLETFEHPNVVRLFDVCTVSRTDRETKLTLVFEHVDQDLTTYLDKVPEPGVPTETIKDMMFQLLRGLDFLHSHRVVHRDLKPQNILVTSSGQIKLADFGLARIYSFQMALTSVVVTLWYRAPEVLLQSSYATPVDLWSVGCIFAEMFRRKPLFRGSSDVDQLGKILDVIGLPGEEDWPRDVALPRQAFHSKSAQPIEKFVTDIDELGKDLLLKCLTFNPAKRISAYSALSHPYFQDLERCKENLDSHLPPSQNTSELNTA 571733EE6BE7FD4A cdk1_mouse Cyclin-dependent kinase 1 p34 protein kinase Cell division protein kinase 1 Cdk1 Cdc2a Cdc2 Cell division control protein 2 homolog Cdkn1 protein protein mouse Mus musculus Mouse MEDYIKIEKIGEGTYGVVYKGRHRVTGQIVAMKKIRLESEEEGVPSTAIREISLLKELRHPNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQFMDSSLVKSYLHQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLVYDPAKRISGKMALKHPYFDDLDNQIKKM DIP protein P11440 original sequence version: 149 DIP protein P11440 original sequence version: 164 9C399FCC41BA7F31 h10_human Histone H1.0 H1-0 H1F0 H1FV Histone H1' Histone H1(0) protein protein human Homo sapiens Human MTENSTSAPAAKPKRAKASKKSTDHPKYSDMIVAAIQAEKNRAGSSRQSIQKYIKSHYKVGENADSQIKLSIKRLVTTGVLKQTKGVGASGSFRLAKSDEPKKSVAFKKTKKEIKKVATPKKASKPKKAASKAPTKKPKATPVKKAKKKLAATPKKAKKPKTVKAKPVKASKPKKAKPVKPKAKSSAKRAGKKK DIP protein P07305 original sequence version: 79 DIP protein P07305-1 original sequence version: 157 03ED6F01919F8BE1 ccnd1_human G1/S-specific cyclin-D1 CCND1 BCL1 PRAD1 PRAD1 oncogene BCL-1 oncogene B-cell lymphoma 1 protein protein protein human Homo sapiens Human MEHQLLCCEVETIRRAYPDANLLNDRVLRAMLKAEETCAPSVSYFKCVQKEVLPSMRKIVATWMLEVCEEQKCEEEVFPLAMNYLDRFLSLEPVKKSRLQLLGATCMFVASKMKETIPLTAEKLCIYTDNSIRPEELLQMELLLVNKLKWNLAAMTPHDFIEHFLSKMPEAEENKQIIRKHAQTFVALCATDVKFISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI DIP protein P24385 original sequence version: 163 DIP protein P24385 original sequence version: 165 DIP protein P24385 original sequence version: 160 DIP protein P24385 original sequence version: 121 DIP protein P24385 original sequence version: 122 DIP protein P24385 original sequence version: 143 DIP protein P24385 original sequence version: 127 3CC00C9905F58D3A cd5r1_human Cyclin-dependent kinase 5 activator 1 Cyclin-dependent kinase 5 regulatory subunit 1 TPKII regulatory subunit CDK5R1 CDK5R NCK5A protein protein human Homo sapiens Human MGTVLSLSPSYRKATLFEDGAATVGHYTAVQNSKNAKDKNLKRHSIISVLPWKRIVAVSAKKKNSKKVQPNSSYQNNITHLNNENLKKSLSCANLSTFAQPPPAQPPAPPASQLSGSQTGGSSSVKKAPHPAVTSAGTPKRVIVQASTSELLRCLGEFLCRRCYRLKHLSPTDPVLWLRSVDRSLLLQGWQDQGFITPANVVFLYMLCRDVISSEVGSDHELQAVLLTCLYLSYSYMGNEISYPLKPFLVESCKEAFWDRCLSVINLMSSKMLQINADPHYFTQVFSDLKNESGQEDKKRLLLGLDR DIP protein Q15078 original sequence version: 119 DIP protein Q15078 original sequence version: 130 DIP protein Q15078 original sequence version: 109 D1C29A07AFF1B644 cdk1-7 1 26 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 18 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate tandem tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association f1a cdk2-uchl1-1 1 17 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 18 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate tandem tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association f1a cdk4-uchl1-1 1 24 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 18 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate tandem tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association f1a cdk6-uchl1-1 1 25 unspecified role unspecified role bait bait purified purified 18 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate tandem tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells physical association physical association f1a cdk1-8 6 15 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 12 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 18 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed P09936:p.Cys90Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain C S flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P09936:p.Ile93Met mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain I M 21 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level association association F1C cdk1-9 2 16 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 24 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 26 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level association association F1E uchl1-cdk4-7 5 8 unspecified role unspecified role prey prey purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range S-tag S-tag s tag s tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 18 unspecified role unspecified role bait bait purified purified n-terminus gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction F3A uchl1-cdk4-1 6 12 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 18 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Required to bind required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association F3C-E uchl1-cdk4-2 6 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed gfp tag gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 18 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association F4B uchl1-cdk4-3 4 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 18 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association F5G uchl1-cdk4-4 4 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 18 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association F5G We observed that overexpression of CDK5 attenuated the interaction between overexpressed CDK4 and UCH-L1 (1.5-fold decrease) (Fig. 5G, lanes 2 and 4). uchl1-cdk2-1 6 23 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 18 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association SF1 uchl1-cdk4-5 6 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 28 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association SF3A Coimmunoprecipitation assays showed that UCH-L1 does not affect interaction levels between CDK4 and cyclin D, CDK5 and p35, and CDK1 and cyclin B (supplemental Fig. S3, A–C). cdk4-ccnd1-28 6 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 29 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association SF3B Coimmunoprecipitation assays showed that UCH-L1 does not affect interaction levels between CDK4 and cyclin D, CDK5 and p35, and CDK1 and cyclin B (supplemental Fig. S3, A–C). cdk5-cdk5r1-13 6 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 14 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association SF3C Coimmunoprecipitation assays showed that UCH-L1 does not affect interaction levels between CDK4 and cyclin D, CDK5 and p35, and CDK1 and cyclin B (supplemental Fig. S3, A–C). cdk1-ccnb1-6 6 20 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association SF3D We also tested whether UCH-L1 competes with the CDK inhibitors p27 (Kip1) or p21 (Cip1) for binding to CDKs. UCH-L1 did not affect the level of interaction between CDK1 and p21, CDK1 and p27, CDK4 and p21, and CDK4 and p27 (supplemental Fig. S3, D and E), indicating that UCH-L1 does not compete with p27 or p21 for the binding to CDKs. cdkn1a-cdk4-1 6 19 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association SF3D We also tested whether UCH-L1 competes with the CDK inhibitors p27 (Kip1) or p21 (Cip1) for binding to CDKs. UCH-L1 did not affect the level of interaction between CDK1 and p21, CDK1 and p27, CDK4 and p21, and CDK4 and p27 (supplemental Fig. S3, D and E), indicating that UCH-L1 does not compete with p27 or p21 for the binding to CDKs. cdk5-cdk5r1-14 4 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q00535:p.Asp144Asn mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain D N Thu Jan 06 20:11:32 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'P09936:p.Asp144Asn' 18 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association SF8 We confirmed that CDK5D144N interacted with UCH-L1 at a level comparable with that with CDK5WT (supplemental Fig. S8). uchl1-cdk4-6 6 12 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 18 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed P09936:p.Glu206Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A P09936:p.Glu174Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain E A P09936:p.Arg63Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain R A P09936:p.Asp176Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain D A P09936:p.His185Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain H A P09936:p.Arg213Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R A P09936:p.Arg207Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R A P09936:p.Lys83Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A P09936:p.Lys123Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A P09936:p.Lys131Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A P09936:p.Lys15Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A P09936:p.Lys221Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A P09936:p.Lys135Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A P09936:p.Lys199Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A P09936:p.Lys221Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A P09936:p.Lys64Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A P09936:p.Lys65Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A P09936:p.Lys71Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A P09936:p.Asp155Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain D A P09936:p.Asp169Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain D A P09936:p.Asp191Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain D A P09936:p.His143Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain H A P09936:p.His59Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain H A P09936:p.Gln58Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Q A P09936:p.Gln66Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Q A P09936:p.Gln66Lys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Q K P09936:p.Ile67Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain I A P09936:p.Phe62His mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain F H P09936:p.Asn184Lys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain N K P09936:p.Val183Lys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain V K flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P09936:p.Glu11Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A P09936:p.Glu37Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A P09936:p.Glu60Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A P09936:p.Glu68Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A P09936:p.Glu109Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A P09936:p.Glu120Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A P09936:p.Glu127Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A P09936:p.Glu149Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A P09936:p.Glu203Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A P09936:p.Glu208Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A Thu Jan 06 19:24:55 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'P09936:p.Glu208Ala' physical association physical association SF5A uchl1-cdk4-8 4 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 13 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed P00441:p.Ala5Val mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain A V flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association SF2A cdkn1b-cdk1-1 6 19 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association SF3E We also tested whether UCH-L1 competes with the CDK inhibitors p27 (Kip1) or p21 (Cip1) for binding to CDKs. UCH-L1 did not affect the level of interaction between CDK1 and p21, CDK1 and p27, CDK4 and p21, and CDK4 and p27 (supplemental Fig. S3, D and E), indicating that UCH-L1 does not compete with p27 or p21 for the binding to CDKs. cdkn1a-cdk1-1 6 20 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association SF3E We also tested whether UCH-L1 competes with the CDK inhibitors p27 (Kip1) or p21 (Cip1) for binding to CDKs. UCH-L1 did not affect the level of interaction between CDK1 and p21, CDK1 and p27, CDK4 and p21, and CDK4 and p27 (supplemental Fig. S3, D and E), indicating that UCH-L1 does not compete with p27 or p21 for the binding to CDKs. uchl1-cdk1-1 6 22 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 18 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association F1D ccnb1-cdk1-9 3 176 enzyme enzyme neutral component neutral component purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 178 C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 180 ecoli Escherichia coli (strain K12) 18 stimulator stimulator neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 27 enzyme target enzyme target substrate substrate neutral component neutral component purified purified phosres phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined resulting-ptm resulting-ptm resulting ptm resulting-ptm ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation F2A Intriguingly, a kinase assay in which purified CDK1-cyclin B, histone H1, and UCH-L1 were mixed in vitro revealed that UCH-L1 enhanced the phosphorylation of histone H1 by CDK1-cyclin B (1.6-fold increase at 30 min) ccnb1-cdk1-10 3 176 enzyme enzyme neutral component neutral component purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 180 n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 178 ecoli Escherichia coli (strain K12) 27 enzyme target enzyme target substrate substrate neutral component neutral component purified purified phosres phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined resulting-ptm resulting-ptm resulting ptm resulting-ptm ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation F2A ccnb1-cdk1-11 3 192 enzyme enzyme neutral component neutral component purified purified 10 enzyme target enzyme target substrate substrate neutral component neutral component purified purified phosres phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined resulting-ptm resulting-ptm resulting ptm resulting-ptm ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation F2B cyclind1_cdk4_human-h1_0-1 3 18 stimulator stimulator neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 10 enzyme target enzyme target substrate substrate neutral component neutral component purified purified phosres phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined resulting-ptm resulting-ptm resulting ptm resulting-ptm ecoli Escherichia coli (strain K12) 192 enzyme enzyme neutral component neutral component purified purified phosphorylation phosphorylation reaction phosphorylation F2B cyclind1_cdk4_human Cyclin D1: CDK4 CCND1:CDK4 Cyclin D1-CDK4 complex 28 unspecified role unspecified role cdk4 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 enzyme enzyme ccnd1 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 201 203 direct interaction direct interaction human Homo sapiens Human stable complex stable complex physical interaction evidence used in manual assertion physical interaction evidence used in manual assertion IPI inferred from physical interaction Cyclin-dependent protein kinase activity. Required for G1 to S phase transition of the mitotic cell cycle. Phosphorylation of Rb proteins by cyclin D:CDK4 complexes leads to their partial inactivation which allows transcription of E2F-controlled genes such as cyclin E1, which activates the downstream Cdk2 kinase. Complex formation enables substrate binding to the kinase, leads to a rearrangement of the catalytic site and exposes Thr-172 of CDK4 for phosphorylation and subsequent activation. Heterodimer created with intact-jami-dbscripts cyclinb1_cdk1_human Cyclin B1: CDK1 Cyclin B1: CDC2 complex Maturation promoting factor MPF Cyclin B1-CDK1 complex CCNB1:CDK1 11 unspecified role unspecified role cdk1 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 enzyme enzyme ccnb1 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 204 205 direct interaction direct interaction human Homo sapiens Human stable complex stable complex physical interaction evidence used in manual assertion physical interaction evidence used in manual assertion IPI inferred from physical interaction Cyclin-dependent protein kinase complex. Required for G2 to M phase transition of the mitotic cell cycle. Complex formation enables substrate binding to the kinase, leads to a rearrangement of the catalytic site and exposes Thr-161 of CDK1 for phosphorylation and subsequent activation. Heterodimer created with intact-jami-dbscripts