IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Protein-peptide complex crystallization: a case study on the ERK2 mitogen-activated protein kinase. Protein-peptide complex crystallization: a case study on the ERK2 mitogen-activated protein kinase. Acta crystallographica. Section D, Biological crystallography 2013 imex curation Gógl G., Törő I., Reményi A., Gógl G., Törő I., Reményi A., Gógl G., Törő I., Reményi A., Gógl G., Törő I., Reményi A. remenyi@elte.hu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Gógl G., Törő I., Reményi A., Gógl G., Törő I., Reményi A., Gógl G., Törő I., Reményi A., Gógl G., Törő I., Reményi A. remenyi@elte.hu Accepted 2021-MAR-16 AT 17:29 GMT AT 17:29 GMT by PPORRAS Acta crystallographica. Section D, Biological crystallography 2013 Only protein-protein interactions imex curation imex curation mk01_human Mitogen-activated protein kinase 1 MAPK1 ERK2 PRKM1 Extracellular signal-regulated kinase 2 Mitogen-activated protein kinase 2 MAP kinase isoform p42 ERT1 PRKM2 protein protein human Homo sapiens Human MAAAAAAGAGPEMVRGQVFDVGPRYTNLSYIGEGAYGMVCSAYDNVNKVRVAIKKISPFEHQTYCQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPGYRS DIP protein P28482 original sequence version: 173 DIP protein P28482 original sequence version: 103 DIP protein P28482 original sequence version: 101 DIP protein P28482 original sequence version: 131 DIP protein P28482 original sequence version: 156 DIP protein P28482 original sequence version: 166 DIP protein P28482 original sequence version: 144 E85D0B2A5D2D724E ks6a1_human Ribosomal protein S6 kinase alpha-1 RPS6KA1 RSK1 90 kDa ribosomal protein S6 kinase 1 MAPKAPK1A Ribosomal S6 kinase 1 MAP kinase-activated protein kinase 1a protein protein human Homo sapiens Human MPLAQLKEPWPLMELVPLDPENGQTSGEEAGLQPSKDEGVLKEISITHHVKAGSEKADPSHFELLKVLGQGSFGKVFLVRKVTRPDSGHLYAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVLMFEMLTGSLPFQGKDRKETMTLILKAKLGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTPKDSPGIPPSAGAHQLFRGFSFVATGLMEDDGKPRAPQAPLHSVVQQLHGKNLVFSDGYVVKETIGVGSYSECKRCVHKATNMEYAVKVIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDGKHVYLVTELMRGGELLDKILRQKFFSEREASFVLHTIGKTVEYLHSQGVVHRDLKPSNILYVDESGNPECLRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKRQGYDEGCDIWSLGILLYTMLAGYTPFANGPSDTPEEILTRIGSGKFTLSGGNWNTVSETAKDLVSKMLHVDPHQRLTAKQVLQHPWVTQKDKLPQSQLSHQDLQLVKGAMAATYSALNSSKPTPQLKPIESSILAQRRVRKLPSTTL DIP protein Q15418 original sequence version: 111 DIP protein Q15418 original sequence version: 85 765731A4442A53DF mp2k2_human Dual specificity mitogen-activated protein kinase kinase 2 MAP2K2 MEK2 MKK2 PRKMK2 ERK activator kinase 2 MAPK/ERK kinase 2 protein protein human Homo sapiens Human MLARRKPVLPALTINPTIAEGPSPTSEGASEANLVDLQKKLEELELDEQQKKRLEAFLTQKAKVGELKDDDFERISELGAGNGGVVTKVQHRPSGLIMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKEAKRIPEEILGKVSIAVLRGLAYLREKHQIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMAPERLQGTHYSVQSDIWSMGLSLVELAVGRYPIPPPDAKELEAIFGRPVVDGEEGEPHSISPRPRPPGRPVSGHGMDSRPAMAIFELLDYIVNEPPPKLPNGVFTPDFQEFVNKCLIKNPAERADLKMLTNHTFIKRSEVEEVDFAGWLCKTLRLNQPGTPTRTAV 3401D522515C30A5 erk2_aa–peprsk1_sqaa-1 1 2 unspecified role unspecified role neutral component neutral component purified purified N-His N terminal His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P28482:p.Arg77Ala P28482:p.Arg77Ala mutation weakening effect mutation mutation certain certain sequence position certain certain certain sequence position certain R A Thu Apr 22 12:31:43 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'P28482:Arg77Ala' P28482:p.Glu314Ala interaction weakening mutation P28482:p.Glu314Ala mutation mutation certain certain sequence position certain certain certain sequence position certain E A Thu Apr 22 11:56:30 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'P28482:Glu314Ala' binding region binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role neutral component neutral component Q15418:p.Ser719Ala mutation Q15418:p.Ser719Ala mutation mutation certain certain sequence position certain certain certain sequence position certain S A Thu Apr 22 11:34:32 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'Q15418:Ser719Ala' Q15418:p.Gln724Ala mutation Q15418:p.Gln724Ala mutation mutation certain certain sequence position certain certain certain sequence position certain Q A Thu Apr 22 12:39:33 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'Q15418:Gln724Ala' binding region binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain synthetic construct synthetic construct direct interaction direct interaction Table 1, Figure 1 free: 0.224, work: 0.178, observed: 0.180 2.30 A 4h3p erk2_aags–pepmkk2-1 1 4 unspecified role unspecified role neutral component neutral component binding region binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain synthetic construct synthetic construct 2 unspecified role unspecified role neutral component neutral component purified purified binding region binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P28482:p.Arg77Ala P28482:p.Arg77Ala mutation weakening mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R A P28482:p.Cys161Ser P28482:p.Cys161Ser mutation mutation mutation certain certain sequence position certain certain certain sequence position certain C S P28482:p.Ile255Gly P28482:p.Ile255Gly mutation mutation mutation certain certain sequence position certain certain certain sequence position certain I G P28482:p.Glu314Ala P28482:p.Pro314Ala mutation weakening effect mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain E A Thu Apr 22 10:49:51 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'P28482:p.Pro314Ala' ecoli Escherichia coli (strain K12) N-terminal gly-ser chrystallisation artefact direct interaction direct interaction Table 1, Figure 1 free: 0.232, work: 0.181, observed: 0.184 2.20 A