IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The tumour suppressor HACE1 controls cell migration by regulating Rac1 degradation. The tumour suppressor HACE1 controls cell migration by regulating Rac1 degradation. Oncogene 2013 imex curation Castillo-Lluva S., Tan CT., Daugaard M., Sorensen PH., Malliri A. RAC1-GST construct gifted search by Dr Francisco Sánchez-Madrid was found to be of human origin by pmids 14988412 and 8910277 amalliri@picr.man.ac.uk 2021-06-04: Serena Petracchini, Department of Microbiology, Institut Pasteur, Paris Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Castillo-Lluva S., Tan CT., Daugaard M., Sorensen PH., Malliri A. RAC1-GST construct gifted search by Dr Francisco Sánchez-Madrid was found to be of human origin by pmids 14988412 and 8910277 amalliri@picr.man.ac.uk Accepted 2021-JUN-25 AT 09:44 BST AT 09:44 BST by ORCHARD 2021-06-04: Serena Petracchini, Department of Microbiology, Institut Pasteur, Paris Oncogene 2013 Only protein-protein interactions imex curation imex curation The tumour suppressor HACE1 controls cell migration by regulating Rac1 degradation. The tumour suppressor HACE1 controls cell migration by regulating Rac1 degradation. Oncogene 2013 imex curation Castillo-Lluva S., Tan CT., Daugaard M., Sorensen PH., Malliri A. RAC1-GST construct gifted search by Dr Francisco Sánchez-Madrid was found to be of human origin by pmids 14988412 and 8910277 amalliri@picr.man.ac.uk 2021-06-04: Serena Petracchini, Department of Microbiology, Institut Pasteur, Paris Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Castillo-Lluva S., Tan CT., Daugaard M., Sorensen PH., Malliri A. RAC1-GST construct gifted search by Dr Francisco Sánchez-Madrid was found to be of human origin by pmids 14988412 and 8910277 amalliri@picr.man.ac.uk Accepted 2021-JUN-25 AT 09:44 BST AT 09:44 BST by ORCHARD 2021-06-04: Serena Petracchini, Department of Microbiology, Institut Pasteur, Paris Oncogene 2013 Only protein-protein interactions imex curation imex curation The tumour suppressor HACE1 controls cell migration by regulating Rac1 degradation. The tumour suppressor HACE1 controls cell migration by regulating Rac1 degradation. Oncogene 2013 imex curation Castillo-Lluva S., Tan CT., Daugaard M., Sorensen PH., Malliri A. RAC1-GST construct gifted search by Dr Francisco Sánchez-Madrid was found to be of human origin by pmids 14988412 and 8910277 amalliri@picr.man.ac.uk 2021-06-04: Serena Petracchini, Department of Microbiology, Institut Pasteur, Paris Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Castillo-Lluva S., Tan CT., Daugaard M., Sorensen PH., Malliri A. RAC1-GST construct gifted search by Dr Francisco Sánchez-Madrid was found to be of human origin by pmids 14988412 and 8910277 amalliri@picr.man.ac.uk Accepted 2021-JUN-25 AT 09:44 BST AT 09:44 BST by ORCHARD 2021-06-04: Serena Petracchini, Department of Microbiology, Institut Pasteur, Paris Oncogene 2013 Only protein-protein interactions imex curation imex curation The tumour suppressor HACE1 controls cell migration by regulating Rac1 degradation. The tumour suppressor HACE1 controls cell migration by regulating Rac1 degradation. Oncogene 2013 imex curation Castillo-Lluva S., Tan CT., Daugaard M., Sorensen PH., Malliri A. RAC1-GST construct gifted search by Dr Francisco Sánchez-Madrid was found to be of human origin by pmids 14988412 and 8910277 amalliri@picr.man.ac.uk 2021-06-04: Serena Petracchini, Department of Microbiology, Institut Pasteur, Paris Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Castillo-Lluva S., Tan CT., Daugaard M., Sorensen PH., Malliri A. RAC1-GST construct gifted search by Dr Francisco Sánchez-Madrid was found to be of human origin by pmids 14988412 and 8910277 amalliri@picr.man.ac.uk Accepted 2021-JUN-25 AT 09:44 BST AT 09:44 BST by ORCHARD 2021-06-04: Serena Petracchini, Department of Microbiology, Institut Pasteur, Paris Oncogene 2013 Only protein-protein interactions imex curation imex curation The tumour suppressor HACE1 controls cell migration by regulating Rac1 degradation. The tumour suppressor HACE1 controls cell migration by regulating Rac1 degradation. Oncogene 2013 imex curation Castillo-Lluva S., Tan CT., Daugaard M., Sorensen PH., Malliri A. RAC1-GST construct gifted search by Dr Francisco Sánchez-Madrid was found to be of human origin by pmids 14988412 and 8910277 amalliri@picr.man.ac.uk 2021-06-04: Serena Petracchini, Department of Microbiology, Institut Pasteur, Paris Only protein-protein interactions imex curation canlf-mdck_ii madin darby canine kidney cell mdck_ii Canine Cocker Spaniel kidney epithelial cell line II kidney kidney [Renal] confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Castillo-Lluva S., Tan CT., Daugaard M., Sorensen PH., Malliri A. Cells were transfected with fluorescent HaloTag-TMR Ligand to enable visualisation of RAC1 RAC1-GST construct gifted search by Dr Francisco Sánchez-Madrid was found to be of human origin by pmids 14988412 and 8910277 amalliri@picr.man.ac.uk Accepted 2021-JUN-25 AT 09:44 BST AT 09:44 BST by ORCHARD 2021-06-04: Serena Petracchini, Department of Microbiology, Institut Pasteur, Paris Oncogene 2013 Only protein-protein interactions imex curation imex curation The tumour suppressor HACE1 controls cell migration by regulating Rac1 degradation. The tumour suppressor HACE1 controls cell migration by regulating Rac1 degradation. Oncogene 2013 imex curation Castillo-Lluva S., Tan CT., Daugaard M., Sorensen PH., Malliri A. RAC1-GST construct gifted search by Dr Francisco Sánchez-Madrid was found to be of human origin by pmids 14988412 and 8910277 amalliri@picr.man.ac.uk 2021-06-04: Serena Petracchini, Department of Microbiology, Institut Pasteur, Paris Only protein-protein interactions imex curation canlf-mdck_ii madin darby canine kidney cell mdck_ii Canine Cocker Spaniel kidney epithelial cell line II kidney kidney [Renal] tap tandem affinity purification TAP TAP tap ms participant Identification by mass spectrometry ms participant Castillo-Lluva S., Tan CT., Daugaard M., Sorensen PH., Malliri A. RAC1-GST construct gifted search by Dr Francisco Sánchez-Madrid was found to be of human origin by pmids 14988412 and 8910277 amalliri@picr.man.ac.uk Accepted 2021-JUN-25 AT 09:44 BST AT 09:44 BST by ORCHARD 2021-06-04: Serena Petracchini, Department of Microbiology, Institut Pasteur, Paris Oncogene 2013 Only protein-protein interactions imex curation imex curation f2z4p3_canlf 60S ribosomal protein L23 RPL23 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MSKRGRGGSSGAKFRISLGLPVGAVINCADNTGAKNLYIISVKGIKGRLNRLPAAGVGDMVMATVKKGKPELRKKVHPAVVIRQRKSYRRKDGVFLYFEDNAGVIVNNKGEMKGSAITGPVAKECADLWPRIASNAGSIA 807E14139B2EB0B5 e2qtq8_canlf SQOR protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MTPLATAVSGPRARLFACFLRLGTQQASPLQLHTGASLAAKNHYEVLVLGGGSGGITMAARMKRRVGADNVAVVEPSERHFYQPIWTLVGAGAKQLSSSGRPTASVIPSGVEWIKARVVELNPDKNCIHTDTGKEISYKYLIIALGIQLDYEKIKGLPEGFDHPKIGSNYSVKTVEKTWKALQDFKEGNAIFTFPNTPVKCAGAPQKIMYLSEAYFRKTGKRSKANIIFNTSLGTIFGVKKYAAALQEIIRERNLTVNYKQNLIEVRADRQEAVFENLDKPGETQVISYEMLHVTPPMSSPDVLKASPVADAVGWVDVDKETLQHKKYPNVFGIGDCTNLPTSKTAAAVAAQSGILDRTISLIMKNKKPTKKYDGYTSCPLVTGYNRVILAEFDYNAQPLETFPFDQSRERLTMYLMKADLMPFLYWNMMLRGYWGGPAFLRKLFHLGMS BF0C52E7C9784266 rac1_human Ras-related C3 botulinum toxin substrate 1 RAC1 MIG5 p21-Rac1 Ras-like protein TC25 Cell migration-inducing gene 5 protein TC25 protein protein human Homo sapiens Human MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL ACEDF83A45E5EA67 a0a5f4d968_canlf LMNA protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MGRRGGASGGRRVSHPMGTSLSPPPPFLDLPAWRFLSSSCPLPTHPHPLARPSSTQEGPSPRGQASGLDREPGAGGIQEGEAPGPAGARRQPAGPRAPTPSGLCPLRDLCPAGSPATLPAMETPSQRRATRSGAQASSTPLSPTRITRLQEKEDLQELNDRLAVYIDRVRSLETENAGLRLRITESEEVVSREVSGIKAAYEAELGDARKTLDSVAKERARLQLELSKVREEFKELKARNTKKEGDLMAAQARLKDLEALLNSKEAALSTALSEKRTLEGELHDLRGQVTKLEAALGEAKKQLQDEMLRRVDAENRLQTLKEELDFQKNIYSEELRETKRRHETRLVEIDNGKQREFESRLADALQELRAQHEDQVEQYKKELEKTYSAKLDNARQSAERNSNLVGAAHEELQQSRIRIDSLSAQLSQLQKQLAAKEAKLRDLEDSLARERDTSRRLLADKEREMAEMRARMQQQLDEYQELLDIKLALDMEIHAYRKLLEGEEERLRLSPSPTSQRSRGRASSHSSQTQGTGSITKKRKLESSESRSSSFSQHARTSGRVAVEEVDEEGKFVRLRNKSSEDQSMGNWQIKRQNGDDPLLTYRFPPKFTLKAGQVVTIWAAGAGATHSPPTDLVWKAQNTWGCGNSLRTALINSTGEEVAMRKLVRSVTVVEDDEDEDGDDLLHHHHGSHCSSSGDPAEYNLRSRTVLCGTCGQPADKASASSSGAQVGGSISSGSSASSVTVTRSYRSVGGSGGGSFGDSLVTRSYLLGSSSPRTQSPQNCSIM 6E59AC98BCC8ACB6 hace1_human E3 ubiquitin-protein ligase HACE1 KIAA1320 HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1 HACE1 HECT-type E3 ubiquitin transferase HACE1 protein protein human Homo sapiens Human MERAMEQLNRLTRSLRRARTVELPEDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVSDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA 8AEC09D9D29DC1D8 e2qv02_canlf CDK17 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MKKFKRRLSLTLRGSQTIDESLSELAEQMTIEENSSKDNEPIVKNGRPPTSHSMHSFLHQYTGSFKKPPLRRPHSVIGGSLGSFMAMPRNGSRLDIVHENLKMGSDGESDQASGTSSDEVQSPTGVCLRNRIHRRISMEDLNKRLSLPADIRIPDGYLEKLQINSPPFDQPMSRRSRRASLSEIGFGKMETYIKLEKLGEGTYATVYKGRSKLTENLVALKEIRLEHEEGAPCTAIREVSLLKDLKHANIVTLHDIVHTDKSLTLVFEYLDKDLKQYMDDCGNIMSMHNVKLFYSEFLRGWAYHREKVLHRDLKPQNLLINEKGELKLADFGLARAKSVPTKTYSNEVVTLWYRPPDVLLGSSEYSTQIDMWGVGCIFFEMASGRPLFPGSTVEDELHLIFRLLGTPSQETWPGVSSNDEFKNYNFPKYKPQPLINHAPRLDSEGIELITKFLQYESKKRVSAEEAMKHVYFRSLGPRIHTLPESVSIFSLKEIQLQKDPGFRNSSYPETGHGKNRRQSMLF C5A291AD3FE92D7E a0a5f4cuj9_canlf ITPRID2 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris ASLDEQSSNTLKGVLVRNGGSFEDDLSLGAEANHLHETDTQIENCNNILAKERRLQFHQKGRSMNSTGSGKSSGTVSSVSELLELYEEDPEEILYNLGFGRDEPDIASKIPSRFFNSSSFARGIDIKVFLSAQMQRMEVENPNYALTSRFRQIEVLTTVANAFSSLYSQVSGTPLQRIGSMSSVTSNKETDSPPPLTRSNTANRLMKTLSKLNLCVDKTERGEGSSPSSAIEKGKIPNVSVMEESGSKNDQKSQKIVKKKDSSSMLATVKEEVSGSSATVMESAGLCDEANSDFNQKTESEQSKETQNHEMKLGEESGILESDLGNDFNTPSHSEPENGNVMKSIHVSTPEKEPCAPLTIPSIRNIMAQQKDSFEMEEVQSTEGEAPHVPAAYQLGLTKSKRDHLLRTASQHSDSSGFAEDSTDCLSLNHLQVPESLQAMGSSADSCDSETTVTSFGEELVTPTAQDQPYFNESEEESLSPLPKGRDKGREQAAPAAAKRSDSQDSPRREPAAQAGSTQSTCGAGDHTSEVTEAGQGLPPAQPADLPREASAESDVDKSSECEFAHYTTHHILKSLASIEAGHGEKGSESAPGPPSSADRVNTALQRAQMKVCSLSHQRVGRSLIKSKDLLKQRYFFAKAGYPLRRSQSLPTTLLSPVRVVSSVNVRLSPGKETRCSPPSFTYKYTPEEEQELGKEVAEPDGQSLVKSTIFISPSSARKEAACPSELTRLEECRPRRTPAPASQSTCSLHSVRSEWPERPLCEHTRTLSTHSVPSVSGAACSAFAAPFGCPHSHRPAAYPYRACPVNPPSAVEMQLRRVLHDIRNSLQNLSQYPMTRGSDLAAAPYSTQKSSVLPLYENTFQELQVMRRSLNLFRTQMMDLELAMLRQQTMVYHHMTEEERYEVDQLQSLRNSVRMELQDLELQLEERLLALEEQFRVVRMPSPFRSSALMGMCGSRSADNLSCPSPLNVMEPVTELMREQSYLKSELGMGLGEMGFEIPPGESSESVFSQATSESSSVCSGPSHANRRTGVPSSDLVGKPKTHLVPSKKVFRASVALTPTAPSRTGSVQTPPDVESSEEVGAAEEASEFVGPKSEVEEGNGKVPLMTAAEEMHKNVEQDELQQVIREIKESIVGEIRREIVSGLLAAVSSSKASNSKQDSH A582E791619B3194 z4yhi2_canlf ACTG1 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MEEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWEDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKTNGEKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 442E9B2E8F4DE719 e2qz61_canlf ATAD3A protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MSWLFGIKGPKGEGAGPPLSLPPAQPGAEGGGDRGGGDRPAPKDKWSNFDPTGLERAAKAARELEHSRHAKEALSLAQMQEQTLQLEQQSKLKEYEAAVEQLKSEQIRVQAEERRKTLSEETRQHQARAQYQDKLARQRYDDQLKQQQLLNEENLRKQEESVQKQEAMRRATVEREMELRHKNEMLRVEAEARARAKAERENADIIREQIRLKAAEHRQTILESIRTAGTLFGEGFRTFVTDWDKVTATVAGLTLLALGVYSAKNATSVAGRYIEARLGKPSLVRETSRITVLEALRHPVQVSRRLLSKPQDALEGVVLSPSLEARVRDIAIATRNTMKNRSLYRNILAYGPPGTGKTLFAKKLALHSGMDYAIMTGGDVAPMGRDGVTAMHKVFDWASTSRRGLLLFVDEADAFLRKRATEKISEDLRATLNAFLHRTGQHSSKFMLVLASNQPEQFDWAVNDRIDEMVRFDLPGREERERLVRMYFDKYVLKPATEGKQRLKLAQFDYGKKCSEIAQLTEGMSGREISQLAVAWQAMAYASEDGVLTEAMMDARVQDAMQQHQQKMQWLKAEGTRPEGAQFLIPSTQAE 1FB26C59C90EAC9E actb_canlf Actin, cytoplasmic 1 ACTB Beta-actin protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF mint Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells 6AFD05CA94E360E2 f1py11_canlf PKP3 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris TLQAGFSSRSQGLGGDPSTFWPIAKPTYSPASWSSRSAVDLSCSRRLSSAHNGGSTLGGAGYGGPQPAAPARPVSFHERAGPGGRADYDTLSLRSLRLGAAGLDDRYSVVSEQLEPPAASAYRALAYERQAACGRMQGPDWPDASEGPPGRPIRAPAMRTLQRFQSSHRSRGTAGVAPGAALEPVARAPSVRSLSLSLADSGHLPDVRGLDSYGGHRSLQRLSSGFDDIDLPSAVKYLMASDPNLQVLGAAYIQHKCYSDAAAKKQARSLQAVPRLVKLFNHANQEVQRHATGAMRNLIYDNADNKLALVEENGIFELLRTLREQDDELRKNVTGILWNLSSSDHLKDRLARDTLEQLTDLVLSPLSGAGGPPLIQQNASEAEIFYNATGFLRNLSSASQATRQKMRECHGLVDALVTYINHALDVGKCEDKSVENAVCVLRNLSYRLYDEMPPSALQRLEGRGRRDVVGAPPGEVVGCFTPQSRRLRELPLTADALTFAEVSKDPKGLEWLWSPQIVGLYNRLLQRCELNPHTTEAAAGALQNITAGDRRWAGVLSRLALEQERILNPLLDRVRTADHHQLRSLTGLIRNLSRNARNKDEMSTKVVSHLIEKLPGSVGEKCPPADVLVNIIAVLNNLVVASPIAARDLLYFDGLRKLVFIKKKRDSPDSEKSSRAASSLLANLWQYSKLHRDFRAKGYRKEDFLGP ADBCDB998C4C47CB cav1_human Caveolin-1 CAV1 CAV protein protein human Homo sapiens Human MSGGKYVDSEGHLYTVPIREQGNIYKPNNKAMADELSEKQVYDAHTKEIDLVNRDPKHLNDDVVKIDFEDVIAEPEGTHSFDGIWKASFTTFTVTKYWFYRLLSALFGIPMALIWGIYFAILSFLHIWAVVPCIKSFLIEIQCISRVYSIYVHTVCDPLFEAVGKIFSNVRINLQKEI DIP protein Q03135 original sequence version: 164 DIP protein Q03135 original sequence version: 119 DIP protein Q03135 original sequence version: 122 DIP protein Q03135-1 original sequence version: 122 DIP protein Q03135 original sequence version: 133 DIP protein Q03135 original sequence version: 155 DIP protein Q03135 original sequence version: 147 2424DE9B5E6521D5 a0a5f4dks9_canlf KIF1C protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MTFGRKARGPGAGSLRGWLAGEGSGDASSWGKMGGGIWVAPGSLTTSSLSVLQLRRVRGSGTMAGASVKVAVRVRPFNARETSQDAKCVVSMQGSTTSIINPKQSKDAPKSFTFDYSYWSHTSAEDPQFASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGRQEPGQQGIVPQLCEDLFSRVNKNESAQLSYSVEVSYMEIYCERVRDLLNPKSRGSLRVREHPILGPYVQDLSKLAVTSYADIADLMDCGNKARTVAATNMNETSSRSHAVFTIVFTQRCHDQLTGLDSEKVSKISLVDLAGSERADSSGARGMRLKEGANINKSLTTLGKVISALADLQSKKRKSDFIPYRDSVLTWLLKENLGGNSRTAMIAALSPADINYEETLSTLRYADRTKQIRCNAIINEDPNARLIRELQEEVARLRELLLAQGLSASALGGLKVDEGSPGGALPATSSPPAPVSPSHPPAHNGELEPSFSPSAEPQIGPEEAMERLQETEKIIAELNETWEEKLRKTEALRMEREALLAEMGVAVREDGGTVGVFSPKKTPHLVNLNEDPLMSECLLYHIKDGITRVGQVDVDIKLTGQFIREQHCLFRSIPQPDGEVVVTLEPCEGAETYVNGRLVTEPLVLKSGNRIVMGKNHVFRFNHPEQARLERERGVPPPPGPPSEPVDWNFAQKELLEQQGIDIKLEMEKRLQDLENQYRKEKEEADLLLEQQRLYADSDSGDDSDKRSCEESWRLISSLREQLPPTTVQTIVKRCGLPSSGKRRAPRRVYQIPQRRRLQGKDPRWATMADLKMQAVKEICYEVALADFRHGRAEIEALAALKMRELCRTYGKPEGPGDAWRAVARDVWDTVGEEEGGSSGGGGEEGARGAEVEDLRAHIDKLTGILQEVKLQNSSKDRELQALRDRMLRMERVIPLTQDHEDDNEEAAEAAWAASSGSDVAAEEEAPSERAPPAPVRPQSPPLSSWERVSRLMEEDPAFRRGRLRWLKQEQLRLQGLQGAGGRGGGLRRPPARFVPPHDCKLRFPFKSNPQHRESWPGAAAGEAPAAPPPPEEVAPPPAAPARRPPSPRRSHRPRRNSLDGGGRSRGGGAAQPEPQHFQPKKHNYYPQQPQPYPAQRPPGPRYPPYTTPPRMRRQRSAPDLKESGAAV 5C1828720733D416 j9p0d4_canlf Ig-like domain-containing protein protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MKFPSLLLGLLMLWIPGSTGEAVMTQTPLSLAVTPGELATISCRASQSLLRSDGKSYLNWYLQKPGQTPRPLIYEASKRFSGVSDRFSGSGSGTDFTLKISRVEAEDVGVYYCQQSLHFPPTVFQLPTQTSWVSWPSCPHKLIFIHFPVSPF A94B224F24252468 myh9_canlf Myosin-9 Myosin heavy chain 9 Myosin heavy chain, non-muscle IIa Non-muscle myosin heavy chain IIa MYH9 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MAQQAADKYLYVDKNFINNPLAQADWAAKKLVWVPSDKSGFEPASLKEEVGEEAIVELVENGKKVKVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKEXYYSGLIYTYSGLFCVVINPYKNLPIYSEEIVEMYKGKKRHEMPPHIYAITDTAYRSMMQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKSKKDQGELERQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVNGYIVGANIETYLLEKSRAIRQAKEERTFHIFYYLLSGAGEHLKTDLLLEPYNKYRFLSNGHVTIPGQQDKDMFQETMEAMRIMGIPEEEQMGLLRVISGVLQLGNIVFKKERNTDQASMPDNTAAQKVSHLLGINVTDFTRGILTPRIKVGRDYVQKAQTKEQADFAIEALAKATYERMFRWLVLRINKALDKTKRQGASFIGILDIAGFEIFDLNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIDLIEKPAGPPGILALLDEECWFPKATDKSFVEKVVQEQGTHPKFQKPKQLKDKADFCIIHYAGKVDYKADEWLMKNMDPLNDNIATLLHQSSDKFVSELWKDVDRIIGLDQVAGMSETALPGAFKTRKGMFRTVGQLYKEQLAKLMATLRNTNPNFVRCIIPNHEKKAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRVVFQEFRQRYEILTPNSIPKGFMDGKQACVLMIKALELDSNLYRIGQSKVFFRAGVLAHLEEERDLKITDVIIGFQACCRGYLARKAFAKRQQQLTAMKVLQRNCAAYLKLRNWQWWRLFTKVKPLLQVSRQEEEMMAKEEELVKVREKQLAAENRLTEMETLQSQLMAEKLQLQEQLQAETELCAEAEELRARLTAKKQELEEICHDLEARVEEEEERCQHLQAEKKKMQQNIQELEEQLEEEESARQKLQLEKVTTEAKLKKLEEDQIIMEDQNCKLAKEKKLLEDRIAEFTTNLMEEEEKSKSLAKLKNKHEAMITDLEERLRREEKQRQELEKTRRKLEGDSTDLNDQIAELQAQIAELKMQLAKKEEELQAALARVEEEATQKNMALKKIRELESQISELQEDLESERASRNKAEKQKRDLGEELEALKTELEDTLDSTAAQQELRSKREQEVNILKKTLEEEARTHEAQIQEMRQKHSQAVEELAEQLEQTKRVKANLEKAKQTLENERGELANEVKVLQQGKGDSEHKRKKAEAQLQELQVKFTEGERVRTELADKVTKLQVELDNVMGLLTQSDSKSSKLTKDFSALESQLQDTQELLQEENRQKLSLSTKLKQMEDEKNSFKEQLEEEEEAKRNLEKQIATLHAQVTDMKKKMEDGVGCLETAEEAKRKLQKDLEGLGQRYEEKVAAYDKLEKTKTRLQQELDDLLVDLDHQRRTASNLEKKQKKFDQLLAEEKTISAKYAEERDRAEAEAREKETKALSLARALEEAMEQKAELERLNKQFRTEMEDLMSSKDDVGKSVHELEKSKRALEQQVEEMKTQLEELEDELQATEDAKLRLEVNLQAMKAQFERDLQGRDEQSEEKKKQLVRQVREMEAELEDEKKQRSMAVAARKKLEMDLKDLEAHIDSANKNRDEAIKQLRKLQAQMKDCVRELDDTRASREEILAQAKENEKKMKSMEAEMIQLQEELAAAERAKRQAQQERDELADEIANSSGKGALALEEKRRLEARIAQLEEELEEEQGNTELVNDRLKKANLQIDQINTDLNLERSHAQKNENARQQLERQNKELKVKLQEMEGTVKSKYKASITALEAKIAQLEEQLDNETKERQAACKQVRRAEKKLKDVLLQVDDERRNAEQFKDQADKASTRLKQLKRQLEEAEEEAQRANASRRKLQRELEDATETADAMNREVSSLKNKLRRGDLPFVVPRRVARKGAGDCSDEEVDGKADGAEAKAAE 0D287FE27035D521 j9p798_canlf 40S ribosomal protein S15a RPS15A protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF D4AC1E8864E3A184 e2r8c9_canlf MOGS protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MARGERRRRGAPVDGARTAERAARGGPARRGGEARGFAWRAALGVVVLSLALGLSGCWLLEWRRARRAVTLHSAPPSLPPDSSSPAVAPDLFWGTYRPHVYFGMKTRSPKPLLTGLMWAQQGATPGTPKLRHTCEQGDGVGPYGWEFHDGLSFGRQHIQDGALKLTTEFVKRPGGQHGGDWSWRVTVEPQASGTSALPLVSLFFYVVTDGKEVLVPEVGAKGQLKFISGHSSELGDFRFTLMAPTNPGDLAPKYSSYNVFWSSNPGLPLLTEMVKSRLNSWFQHRPPGASPERYLGLPGSLKWEDKGPSGQGQGQGQFLIQQVTLKTPFSVELVFESGSARAGRSQALEQLSGSLLTQALESHAEAFREHFEKTFQLKEKGLSLEEQALGQVALSGLLGGIGYFYGQGLVLADMGVEGSEQKVDPALFPPVPLFTAVPSRSFFPRGFLWDEGFHQLVVQRWDPRLTREVLGHWLGLLNADGWIGREQVLGDEARARVPPEFLVQKAAHANPPTLLLPVAHMLEVGDPADFAFLRRAFPRLCAWFSWLHKSQAGPVPLSYRWRGRDPVLPTLLNPKTLPSGLDDYPRASHPSVSERHLDLRCWMALGARVLLRLAEQLGEAEAAAELGPLAASLEAEENLDELHWAPELGVFADFGNHTKAVQLKPRPPQGLVRVVGRPHPRLQYVDALGYVSLFPLLLRLLDPNSPRLGPLLDVLADSRHLWSPFGLRSLAASSPFYGQRNSEHDPPYWRGAVWLNVNYLALGALYHYGHLEGPHQAQAAKLHRELRTNVVGNVWRQYQATGFLWEQYSDRDGRGMGCRPFQGWTSLVLLAMAEDY 805DDA10BF39237F e2rqh0_canlf SH3BP4 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MAAQRIRAANSSGLPRCKSEGTLIDLSEGFSEASFNDVKVPSPSALLVDNPTPFGNAKEVIAIKDYCPTNFTTLKFSKGDHLYVLDTSGGEWWYAHNTTEMGYIPSSYVQPLNYRNSTLSDSGVIDNLPDSPDEVAKELDLLGVGMDDKGPSKTYSNNPFWNGAQTNPFLNGNVTAMPSVDELMPKSTVDLLLFDTGTSSFTESSSATTNSTGNIFDELQATNRLQAEAPGKRDNPFFRSKRSYSLSELSVLQAKSDAPDSSGFFTGLKSPAPEQFQSREDFRAAWLSHRKLTRSCHDLDLLGQSPGWGQTQAVETSIACKLDSSGGAVQLPDTNISIHVPEGHVAPGETQQISMKALLDPPLELNSDRCSSVSPVLEVKLSTLEVKTYIILEMKVSAEVKNDIFSKSTVGLQCLRSDLKEGPYVPIPLTYSYGDTVQVQLDNLEPCMYLAIVAHGPNILYPSTVWDFINKKITVGLYGPKHIHPSFKTVVTIFGHDCAPKTLLVSEVTRQAPSPAPVALQLWGKHQFVLSRPQDLHVCMFSNMTNYEVKASEQAKVVRGFQMKLGKVSRLIFPIACQNPGELSDFTLRVQVKGDQEAILTQFCVQTPQPPPKSAIKPSGQRRFLKKNEVGKIILSPFAATTKYPTFQDRPVSSLKFGKLLKTVVRQSKNHYLLEYKKGDAIALLSEEKIRLKGQLWTKEWYIGYHQGRVGLVHAKNVLVVGKARPSLLAGPELSTSVLLEQILRPCKFLTYIYASVRTLLMENISSWRSFADALGYGNLPLTFFCRAELDSEPERVASVLEKLKEDCNNTENKDRKSFQKELMMALLKMDCQGLVVRLIQDFVLLTTAVEVAQRWRELAEKLAKVSKQQMDAYESPHRDRNGVVDSEAMWKPAYDFLLTWSHQIGDSYRDVIQELHIGLDKMKNPITKRWKHLTGTLILVNSLDILRAAAFSPVDHDDFVI 40273C049A0F95E3 f1pip0_canlf Enoyl-CoA hydratase HADHA protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MVASRAIGSLSRATASRMFRCPGYVCRNFTGSSALRSRTHINFGVKGDVAVVRLNSPNSKVNTLNKELQSEFMEVMNEIWASDQIRSAVLISTKPGCFIAGADINMLASCKTHEEVTRISQEGQRMFEKLEKSTKPIVAAISGACLGGGLELAISCQYRIATKDRKTVLGAPEVLLGILPGAGGTQRLPKMVGLPAAFDMMLTGRNIRADRAKRMGLVDQLVEPLGPGLKPPEERTIEYLEEVAVNFAKGLSDKKISIKRDKGLVEKLTSYALSIPFVRQQVYKKVEEKVRKQTKGLYPAPLKIIDVVKTGIEQGNDAGYLSESQKFGELAMTKESKALMGLYHGQVLCKKNKFGAPQKEVKDLAILGAGLMGAGIAQVSVDKGLKTILKDATLTGLGRGQQQVFKGLNDKVKKKALTSFERDSIFSNLTGQLDYQGFEKADMVIEAVFEDLSLKHRVLKEVEAVIPGHCVFASNTSALPINEIAAASKRPEKVIGMHYFSPVDKMQLLEIITTDKTSKDTTASAVAVGLKQGKVIIVVKDGPGFYTTRCLAPMMSEVLRILQEGVEPKKLDSLTTSFGFPVGAATLVDEVGVDVAKHVSEDLGKAFGERFEGGNIELLKEMVSRGFLGRKSGKGFYIYQEGVKTKNVNSDMDGILASLKVPPKYGVSSDEDIQYRLVTRFVNEAVLCLQEGILATPTEGDIGAVFGLGFPPCLGGPFRFVDLYGAQKIVDRLRKFEAAYGKQFTPCQLLIDHANSANKFYQ FC4B1D914ED59447 a0a5f4cug8_canlf LMO7 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MSFRPSSPAHTRRTAGDSEYLSPYSATLRAREGEKCEASLRPEPPRRHPAGEAGAFHLPHLEAGRSGGPPGECVCVCVGGGAGMGGARSGRCPHLPPGGPGIPRGRPRTWRRRARRARAAGEGGGAGAARHCGPRSSPGKFLGPGKVQLCAAIPVLVLEGMEEPGADCAVAFAEAQRWVEAVTEKNFETKDFRASLENGVLLCDLVNKLKPGVIKKINRLSTPIAGLDNINVFLKACEQIGLKEAQLFHPGDLQDLSNRVTVRQEETDRRVKNVLITLYWLGRKAQSNPYYNGPYLNLKAFENLLGQALTKALEDTSCLKRSGRDSGYGDIWCAERGDFPTSPGNHKREDSFESLDSLGSRSFTSCSSDITLRGGREGCESDTDSEFTSRMQDYNKDDMSYRRISAIEPKTALPFNRFLPNKSRQPSYVPAPLRKKKPDKNEDNRRSWASPVYPEADGTFPSNERRTWGTNVENCPTVQETSSSSCYLEEEREKTRTPNTIKDDLYVRKLSPIMPNPGNAFDQFLPKCWIPEDVNWKRIKRETYKPWYKEFQGFSQFLLLQALQTYSDDILASETNIKIDPTAGPRLITRRKNLSSAPGCKRDDLEMPTLDPDLENDDFFVRKTGAFHANPYVLRAFEDLRRFSEPDDSVERDIILQCREGELVLPDLEKDDMIVRRIPAQKKEVPLSGAPERYQPVPLPEPWTLPPEIQAKFLCVLERTHPSKEKRKSCGVLVPSSRKKRDDMLTRKIQSWQLGTTVPPVSFTPGPCSEADLRRWEAIREASRLRHRKRLMVERLFQKIYGENGSKSMSDVSAEDIQNLRQLRYEEMQKIKSQLKEQDQKWQDDLAKWKDRRKSYTSDLQKKKEEREEIEKQALEKSERSSKTFKEMLQDRESRNQMSTVTSRRRLHSNDDVLNEEMLSPTLTMSEASYQSERVEEKETMYSTEIPKQDSTTFVKREDAVTTDIQLPSQSPMEEQLPDSLSSQHSPSTRMQSTRVSASLPRSYQKTDTARLTSVVTPRPFGSQTRGISSLPRSYTMDDAWRYNGDVEDDKRTQNSFVSTSVQKPDTSQLASSKSSETEIAAVGEGVMGLPSPTPSFSSLSQDQAATSKATLSSSSGPDLVSELGEGRSSPQTEVSRSQDQFSDMRISINQTPGSGLDFGFTVKWAFSGIFVASVEAGSPAEFSQLQVDDEIIAINNTKFSYKDTKEWEATMANAKETGNLVMDIRRYGKSGSPETKWIDTTSGIYNSDKSSSLSITTDFSESLQHSNTESKEINGIRDESNTFESKASEPISLKNLKRRSQFFEQGSSDSVVPDLPVPTLSTPSRWAWDQEEERRRQERWQREQDRLLQEKYQREQEKLREEWQRAQQEAERENYKYLNEERMVLSSHSLSRPTREPAVATWETAWGEGSKSSDREGTRAGEEERRPQQKEDEEEDQRKRLQEQLALERERKLKEQQDQEEQERRRRQEEAQEQKPQAERERETTVKIYQYRRPIDSYDIPKREEQSSGLLPSDRNKSRSTTELDGYPANKNGGNKYSDQIGSTSSSQRSSKKEQAPSEAELERQQILQEMRKRTSLLNDNSWIRQRSSSVNKEPICLPGIMRRGESLDNLDSPRSSSWKPSPWFNQPSGVRASSSVQDFSRPPPQLVSTSNRAYMRNPSSSIPPPSSAGSVQTTTAPSPTPHSPSPSLSQSGSQLRNSVWPASVTSEAPPQEPKSESETINCTATTAISDSNLDGQLPCDVNLHTKALLQIEEEVVSADVDL 85B9F8AD532B25FA rac1_mouse Ras-related C3 botulinum toxin substrate 1 Rac1 p21-Rac1 protein protein mouse Mus musculus Mouse MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL DIP protein P63001 original sequence version: 125 ACEDF83A45E5EA67 rac1-actb-1 6 16 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 7 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 23 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 24 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 20 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 19 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 25 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 22 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 13 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 20 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 12 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 26 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 15 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 21 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 17 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 14 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 27 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association Supplementary Table I hepatocyte growth factor hace1-rac1-2 4 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 27 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 1a rac1-hace1-1 2 9 unspecified role unspecified role bait bait purified purified tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association 1b HACE1 more efficiently binds to GTP-bound RAC1 rac1-hace1-2 1 9 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction 1c rac1-hace1-3 4 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range ankyrin repeat containing domain required to bind necessary binding region required to bind range range range range physical association physical association 1e, S1a, S1c MET receptor tyrosine kinase hace1-rac1-3 5 9 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell halo tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 2g hepatocyte growth factor rac1-hace1-4 4 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association S1b hace1-rac1-4 3 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 18 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association S3j