MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url&gt;http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it A Cdk5-dependent switch regulates Lis1/Ndel1/dynein-driven organelle transport in adult axons. A Cdk5-dependent switch regulates Lis1/Ndel1/dynein-driven organelle transport in adult axons. The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 imex curation Pandey JP., Smith DS. Brain Disease - Neurological Diseases & Disorders deannasm@biol.sc.edu <deannasm@biol.sc.edu>; Only protein-protein interactions imex curation rat-drg dorsal root ganglia drg lumbar dorsal root ganglia anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Pandey JP., Smith DS. Brain Disease - Neurological Diseases & Disorders deannasm@biol.sc.edu <deannasm@biol.sc.edu>; Accepted 2021-JUL-21 AT 11:32 BST AT 11:32 BST by LUANA The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 Only protein-protein interactions imex curation imex curation A Cdk5-dependent switch regulates Lis1/Ndel1/dynein-driven organelle transport in adult axons. A Cdk5-dependent switch regulates Lis1/Ndel1/dynein-driven organelle transport in adult axons. The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 imex curation Pandey JP., Smith DS. Brain Disease - Neurological Diseases & Disorders deannasm@biol.sc.edu <deannasm@biol.sc.edu>; Only protein-protein interactions imex curation chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Pandey JP., Smith DS. Brain Disease - Neurological Diseases & Disorders deannasm@biol.sc.edu <deannasm@biol.sc.edu>; Accepted 2021-JUL-21 AT 11:32 BST AT 11:32 BST by LUANA The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 Only protein-protein interactions imex curation imex curation lis1_rat Platelet-activating factor acetylhydrolase IB subunit alpha PAF acetylhydrolase 45 kDa subunit PAF-AH alpha Lissencephaly-1 protein Pafah1b1 Lis1 Lis-1 Pafaha protein protein rat Rattus norvegicus (Rat) Rat MVLSQRQRDELNRAIADYLRSNGYEEAYSVFKKEAELDMNEELDKKYAGLLEKKWTSVIRLQKKVMELESKLNEAKEEFTSGGPLGQKRDPKEWIPRPPEKYALSGHRSPVTRVIFHPVFSVMVSASEDATIKVWDYETGDFERTLKGHTDSVQDISFDHSGKLLASCSADMTIKLWDFQGFECIRTMHGHDHNVSSVAIMPNGDHIVSASRDKTIKMWEVQTGYCVKTFTGHREWVRMVRPNQDGTLIASCSNDQTVRVWVVATKECKAELREHEHVVECISWAPESSYSSISEATGSETKKSGKPGPFLLSGSRDKTIKMWDVSTGMCLMTLVGHDNWVRGVLFHSGGKFILSCADDKTLRVWDYKNKRCMKTLNAHEHFVTSLDFHKTAPYVVTGSVDQTVKVWECR 4DBF6A24A6B131CD lis1_mouse Platelet-activating factor acetylhydrolase IB subunit beta PAF acetylhydrolase 45 kDa subunit PAF-AH alpha Lissencephaly-1 protein Pafah1b1 Lis1 Lis-1 Pafaha protein protein mouse Mus musculus Mouse MVLSQRQRDELNRAIADYLRSNGYEEAYSVFKKEAELDMNEELDKKYAGLLEKKWTSVIRLQKKVMELESKLNEAKEEFTSGGPLGQKRDPKEWIPRPPEKYALSGHRSPVTRVIFHPVFSVMVSASEDATIKVWDYETGDFERTLKGHTDSVQDISFDHSGKLLASCSADMTIKLWDFQGFECIRTMHGHDHNVSSVAIMPNGDHIVSASRDKTIKMWEVQTGYCVKTFTGHREWVRMVRPNQDGTLIASCSNDQTVRVWVVATKECKAELREHEHVVECISWAPESSYSSISEATGSETKKSGKPGPFLLSGSRDKTIKMWDVSTGMCLMTLVGHDNWVRGVLFHSGGKFILSCADDKTLRVWDYKNKRCMKTLNAHEHFVTSLDFHKTAPYVVTGSVDQTVKVWECR DIP protein P63005 original sequence version: 47 4DBF6A24A6B131CD ndel1_human Nuclear distribution protein nudE-like 1 NDEL1 Mitosin-associated protein 1 EOPA MITAP1 NUDEL protein protein human Homo sapiens Human MDGEDIPDFSSLKEETAYWKELSLKYKQSFQEARDELVEFQEGSRELEAELEAQLVQAEQRNRDLQADNQRLKYEVEALKEKLEHQYAQSYKQVSVLEDDLSQTRAIKEQLHKYVRELEQANDDLERAKRATIVSLEDFEQRLNQAIERNAFLESELDEKESLLVSVQRLKDEARDLRQELAVRERQQEVTRKSAPSSPTLDCEKMDSAVQASLSLPATPVGKGTENTFPSPKAIPNGFGTSPLTPSARISALNIVGDLLRKVGALESKLAACRNFAKDQASRKSYISGNVNCGVLNGNGTKFSRSGHTSFFDKGAVNGFDPAPPPPGLGSSRPSSAPGMLPLSV DIP protein Q9GZM8 original sequence version: 36 DIP protein Q9GZM8 original sequence version: 65 DIP protein Q9GZM8 original sequence version: 78 E91EA053FCA315D7 ndel1_rat Nuclear distribution protein nudE-like 1 Ndel1 Nude2 Nudel protein protein rat Rattus norvegicus (Rat) Rat MDGEDIPDFSSLKEETAYWKELSLKYKQSFQEARDELVEFQEGSRELEAELEAQLVQAEQRNRDLQADNQRLKYEVEALKEKLEHQYAQSYKQVSVLEDDLSQTRAIKEQLHKYVRELEQANDDLERAKRATIVSLEDFEQRLNQAIERNAFLESELDEKESLLVSVQRLKDEARDLRQELAVRERQQEVTRKSAPSSPTLDCEKMDSAVQASLSLPATPVGKGTENSFPSPKAIPNGFGTSPLTPSARISALNIVGDLLRKVGALESKLAACRNFAKDQASRKSYVPGSVNCGVMNSNGPECPRSGRATFFHKGAVNGFDPAPPPPGLGSSRPSSAPGMLPLSV CB3059DFD998E9EC pafah1b1-ndel1-1 2 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P63005:p.Lys147Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Tue Oct 05 18:35:14 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'P63005:p.lys147ala' P63005:p.Arg212Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain R A Tue Oct 05 18:47:01 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'P63005:p.arg212ala' physical association physical association fig. 3b Dynein was detected by an aspecific antibody Anti-DIC 74.1 that recognizes both intermediate chains 1 and 2 of cytosolic Dynein (not suitable for creating a molecule set) so it was not annotated HA-tagged K147A pulls down Ndel1 but not dynein (lanes 2 and 5). HA-tagged R212A pulls down dynein but not Ndel1 (lanes 3 and 6). ndel1-10 1 6 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 3 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association fig. 4c Exposure of DRG cultures to roscovitine, a known inhibitor of Cdk5 (Gray et al., 1999; Kim and Ryan, 2010), reduced the amount of Ndel1 that coprecipitated with Lis1 from DRG culture extracts (Fig. 4C). roscovitine CHEBI:45307