UniProt UniProt http://www.uniprot.org Characterization of the human tRNA-guanine transglycosylase: confirmation of the heterodimeric subunit structure. Characterization of the human tRNA-guanine transglycosylase: confirmation of the heterodimeric subunit structure. RNA (New York, N.Y.) 2010 imex curation Chen YC., Kelly VP., Stachura SV., Garcia GA. gagarcia@umich.edu Only protein-protein interactions imex curation in vitro In vitro crosslink cross-linking study crosslink ms participant Identification by mass spectrometry ms participant Chen YC., Kelly VP., Stachura SV., Garcia GA. Cross-linking agent dimethyl suberimidate (DMS) gagarcia@umich.edu Accepted 2021-JUL-05 AT 03:50 BST AT 03:50 BST by KALPANAP RNA (New York, N.Y.) 2010 Only protein-protein interactions imex curation imex curation Characterization of the human tRNA-guanine transglycosylase: confirmation of the heterodimeric subunit structure. Characterization of the human tRNA-guanine transglycosylase: confirmation of the heterodimeric subunit structure. RNA (New York, N.Y.) 2010 imex curation Chen YC., Kelly VP., Stachura SV., Garcia GA. gagarcia@umich.edu Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) chromatography chromatography technology column chromatography chromatography weight by comassie molecular weight estimation by coomasie staining weight by comassie Chen YC., Kelly VP., Stachura SV., Garcia GA. Complex was purifed using Ni2+ affinity chromatography followed by gel filtration (Sephacryl S-200) gagarcia@umich.edu Accepted 2021-JUL-05 AT 03:50 BST AT 03:50 BST by KALPANAP RNA (New York, N.Y.) 2010 Only protein-protein interactions imex curation imex curation Characterization of the human tRNA-guanine transglycosylase: confirmation of the heterodimeric subunit structure. Characterization of the human tRNA-guanine transglycosylase: confirmation of the heterodimeric subunit structure. RNA (New York, N.Y.) 2010 imex curation Chen YC., Kelly VP., Stachura SV., Garcia GA. gagarcia@umich.edu Only protein-protein interactions imex curation in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Chen YC., Kelly VP., Stachura SV., Garcia GA. gagarcia@umich.edu Accepted 2021-JUL-05 AT 03:50 BST AT 03:50 BST by KALPANAP RNA (New York, N.Y.) 2010 Only protein-protein interactions imex curation imex curation Mock publication and experiment for abstract interactions that are not interaction evidences. Mock publication and experiment for abstract interactions that are not interaction evidences. unspecified method zinc(2+) zinc(2+) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) trna(tyr) tRNA(Tyr) transfer RNA-Tyr tRNA(Tyr) tyrosine tRNA small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) tgt_human Queuine tRNA-ribosyltransferase catalytic subunit 1 tRNA-guanine transglycosylase Guanine insertion enzyme QTRT1 TGT TGUT protein protein human Homo sapiens Human MAGAATQASLESAPRIMRLVAECSRSRARAGELWLPHGTVATPVFMPVGTQATMKGITTEQLDALGCRICLGNTYHLGLRPGPELIQKANGLHGFMNWPHNLLTDSGGFQMVSLVSLSEVTEEGVRFRSPYDGNETLLSPEKSVQIQNALGSDIIMQLDDVVSSTVTGPRVEEAMYRSIRWLDRCIAAHQRPDKQNLFAIIQGGLDADLRATCLEEMTKRDVPGFAIGGLSGGESKSQFWRMVALSTSRLPKDKPRYLMGVGYATDLVVCVALGCDMFDCVFPTRTARFGSALVPTGNLQLRKKVFEKDFGPIDPECTCPTCQKHSRAFLHALLHSDNTAALHHLTVHNIAYQLQLMSAVRTSIVEKRFPDFVRDFMGAMYGDPTLCPTWATDALASVGITLG 1E30F3C7BF47A106 guanine guanine 2-Amino-6-hydroxypurine 2-amino-6-oxopurine G Gua Guanine GUANINE guanine 2-amino-1,9-dihydro-6H-purin-6-one small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) qtrt2_human Queuine tRNA-ribosyltransferase accessory subunit 2 Queuine tRNA-ribosyltransferase domain-containing protein 1 QTRT2 QTRTD1 protein protein human Homo sapiens Human MKLSLTKVVNGCRLGKIKNLGKTGDHTMDIPGCLLYTKTGSAPHLTHHTLHNIHGVPAMAQLTLSSLAEHHEVLTEYKEGVGKFIGMPESLLYCSLHDPVSPCPAGYVTNKSVSVWSVAGRVEMTVSKFMAIQKALQPDWFQCLSDGEVSCKEATSIKRVRKSVDRSLLFLDNCLRLQEESEVLQKSVIIGVIEGGDVMEERLRSARETAKRPVGGFLLDGFQGNPTTLEARLRLLSSVTAELPEDKPRLISGVSRPDEVLECIERGVDLFESFFPYQVTERGCALTFSFDYQPNPEETLLQQNGTQEEIKCMDQIKKIETTGCNQEITSFEINLKEKKYQEDFNPLVRGCSCYCCKNHTRAYIHHLLVTNELLAGVLLMMHNFEHYFGFFHYIREALKSDKLAQLKELIHRQAS C9447FD900DD667B qtrt1-qtrt2-1 2 7 weight by comassie molecular weight estimation by coomasie staining weight by comassie ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 weight by comassie molecular weight estimation by coomasie staining weight by comassie ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction Figs. 2,3 qtrt1-qtrt2-2 1 7 unspecified role unspecified role neutral component neutral component purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction Fig. 4, Supp fig. 3, Supp Table 3 guanine-tgt_human_1-1 3 20 enzyme enzyme unspecified role unspecified role purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9BXR0:p.Asp279Asn mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain D N 23 ecoli Escherichia coli (strain K12) 8 enzyme target enzyme target substrate substrate unspecified role unspecified role region 14c radiolabel 14c radiolabel C14 14C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 enzyme target enzyme target substrate substrate unspecified role unspecified role enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction Figs. 5, 6, Table 1 tgt_human-1 Queuine tRNA-ribosyltransferase complex QTRT1-QTRTD1 transglycosylase complex tRNA-guanine transglycosylase complex QTRT1:QTRT2 TGT complex 5 cofactor cofactor coenzyme qtrt2 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined qtrt1 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 enzyme enzyme zinc binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain qtrt2 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role zinc binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain qtrt1 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 28 33 30 29 34 31 physical association physical association human Homo sapiens Human stable complex stable complex physical interaction evidence used in manual assertion physical interaction evidence used in manual assertion IPI inferred from physical interaction Catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Q34 appears to enhance the speed of translation of C-ending codons but reduced that of U-ending codons. In human cytoplasm, tRNAs for His and Asn have queuosine, whilst in mitochondria, queuosine occurs at the wobble positions of tRNAs for Tyr, His, Asn and Asp. Heterodimer Accepted 2021-JUL-05 by BMELDAL