DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Specific interaction between EF-G and RRF and its implication for GTP-dependent ribosome splitting into subunits. Specific interaction between EF-G and RRF and its implication for GTP-dependent ribosome splitting into subunits. Journal of molecular biology 2007 imex curation Gao N., Zavialov AV., Ehrenberg M., Frank J. joachim@wadsworth.org Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Gao N., Zavialov AV., Ehrenberg M., Frank J. joachim@wadsworth.org Accepted 2021-JUL-21 AT 15:43 BST AT 15:43 BST by LUANA Journal of molecular biology 2007 Only protein-protein interactions imex curation imex curation rl30_ecoli 50S ribosomal protein L30 rpmD Large ribosomal subunit protein uL30 JW3264 b3302 protein protein ecoli Escherichia coli (strain K12) MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAIRGMINAVSFMVKVEE 08F427BBB6F820A4 rl20_ecoli 50S ribosomal protein L20 rplT pdzA b1716 JW1706 Large ribosomal subunit protein bL20 protein protein ecoli Escherichia coli (strain K12) MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQYAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFDKVAFTALVEKAKAALA DIP protein P0A7L3 original sequence version: 54 E60AFDBB6F05DFB3 rl16_ecoli 50S ribosomal protein L16 rplP Large ribosomal subunit protein uL16 JW3275 b3313 protein protein ecoli Escherichia coli (strain K12) MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTARQIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVLYEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM DIP protein P0ADY7 original sequence version: 44 445A9B167F7564D1 rl13_ecoli 50S ribosomal protein L13 rplM Large ribosomal subunit protein uL13 JW3200 b3231 protein protein ecoli Escherichia coli (strain K12) MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEYTPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIEIAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI DIP protein P0AA10 original sequence version: 47 58B45EF9EB75FDCD rl34_ecoli 50S ribosomal protein L34 rpmH rimA ssaF b3703 JW3680 Large ribosomal subunit protein bL34 protein protein ecoli Escherichia coli (strain K12) MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTVSK 600916E398939412 rl31_ecoli 50S ribosomal protein L31 rpmE Large ribosomal subunit protein bL31-A JW3907 b3936 protein protein ecoli Escherichia coli (strain K12) MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFFTGKQRDVATGGRVDRFNKRFNIPGSK D657A681A0B858CF rl15_ecoli 50S ribosomal protein L15 rplO Large ribosomal subunit protein uL15 JW3263 b3301 protein protein ecoli Escherichia coli (strain K12) MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGGGVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEIRLSDLAKVEGGVVDLNTLKAANIIGIQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE DIP protein P02413 original sequence version: 105 DIP protein P02413 original sequence version: null 53D14CD948815FD9 rl24_ecoli 50S ribosomal protein L24 rplX Large ribosomal subunit protein uL24 JW3271 b3309 protein protein ecoli Escherichia coli (strain K12) MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKKHQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSETIK DIP protein P60624 original sequence version: 72 0ABF7DD305FA07F5 rl19_ecoli 50S ribosomal protein L19 rplS Large ribosomal subunit protein bL19 JW2587 b2606 protein protein ecoli Escherichia coli (strain K12) MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFEGVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRERTGKAARIKERLN DIP protein P0A7K6 original sequence version: 52 7BD9F2EFB9DDB06B rl18_ecoli 50S ribosomal protein L18 rplR Large ribosomal subunit protein uL18 JW3266 b3304 protein protein ecoli Escherichia coli (strain K12) MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNGSEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGRVQALADAAREAGLQF DIP protein P0C018 original sequence version: 54 CA1082FF9B5D9697 rl4_ecoli 50S ribosomal protein L4 b3319 rplD eryA JW3281 Large ribosomal subunit protein uL4 protein protein ecoli Escherichia coli (strain K12) MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTRAQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYRGALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLAARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA DIP protein P60723 original sequence version: 78 3A953206B0F083B5 rrf_ecoli Ribosome-recycling factor Ribosome-releasing factor b0172 JW0167 frr rrf protein protein ecoli Escherichia coli (strain K12) MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEYYGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPLPPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDVQKLTDAAIKKIEAALADKEAELMQF DIP protein P0A805 original sequence version: 48 CBFB9675AAD0CFD8 rl35_ecoli 50S ribosomal protein L35 Ribosomal protein A rpmI b1717 JW1707 Large ribosomal subunit protein bL35 protein protein ecoli Escherichia coli (strain K12) MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHLRPKAMVSKGDLGLVIACLPYA 98A72C0AD6CE0A07 rl22_ecoli 50S ribosomal protein L22 rplV eryB b3315 JW3277 Large ribosomal subunit protein uL22 protein protein ecoli Escherichia coli (strain K12) METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAAVLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSHITVVVSDR DIP protein P61175 original sequence version: 97 F0E0C6982772277C rl11_ecoli 50S ribosomal protein L11 rplK relC b3983 JW3946 Large ribosomal subunit protein uL11 protein protein ecoli Escherichia coli (strain K12) MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNAKTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKISRAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED DIP protein P0A7J7 original sequence version: 53 C49226BB2462BE0F rl14_ecoli 50S ribosomal protein L14 rplN Large ribosomal subunit protein uL14 JW3272 b3310 protein protein ecoli Escherichia coli (strain K12) MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIKEAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGPVTRELRSEKFMKIISLAPEVL DIP protein P0ADY3 original sequence version: 46 192ACB1623979510 rl5_ecoli 50S ribosomal protein L5 rplE Large ribosomal subunit protein uL5 JW3270 b3308 protein protein ecoli Escherichia coli (strain K12) MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAIADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFERLITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTAKSDEEGRALLAAFDFPFRK DIP protein P62399 original sequence version: 68 B2E95A8BE4B8D2A7 rl3_ecoli 50S ribosomal protein L3 rplC Large ribosomal subunit protein uL3 JW3282 b3320 protein protein ecoli Escherichia coli (strain K12) MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDGYRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFADVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKKMAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA DIP protein P60438 original sequence version: null EA8E10EDD2C0A8FD rl33_ecoli 50S ribosomal protein L33 rpmG Large ribosomal subunit protein bL33 JW3611 b3636 protein protein ecoli Escherichia coli (strain K12) MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVRQHVIYKEAKIK DIP protein P0A7N9 original sequence version: 53 31681CC3DAC0C5D6 rl29_ecoli 50S ribosomal protein L29 rpmC Large ribosomal subunit protein uL29 JW3274 b3312 protein protein ecoli Escherichia coli (strain K12) MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLKQVRRDVARVKTLLNEKAGA DIP protein P0A7M6 original sequence version: 56 DAEF8F126B0AA077 rl32_ecoli 50S ribosomal protein L32 rpmF Large ribosomal subunit protein bL32 JW1075 b1089 protein protein ecoli Escherichia coli (strain K12) MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHITADGYYRGRKVIAK DIP protein P0A7N4 original sequence version: 45 FA07955DAA8E4E94 rl23_ecoli 50S ribosomal protein L23 rplW Large ribosomal subunit protein uL23 JW3280 b3318 protein protein ecoli Escherichia coli (strain K12) MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIKAAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE DIP protein P0ADZ0 original sequence version: 47 30CD1D77CC7CF9EB rl36_ecoli 50S ribosomal protein L36 rpmJ b3299 JW3261 Ribosomal protein B Large ribosomal subunit protein bL36-A protein protein ecoli Escherichia coli (strain K12) MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG C4785CEC0578EB24 efg_ecoli Elongation factor G fusA fus far b3340 JW3302 protein protein ecoli Escherichia coli (strain K12) MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHDGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERSMRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKTRLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEWHQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQAMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLTFFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVTTGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTDEESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQSGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPLAGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETPEENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTMEFLKYDEAPSNVAQAVIEARGK DIP protein P0A6M8 original sequence version: 51 8C72B9F87253BC7B rl17_ecoli 50S ribosomal protein L17 rplQ Large ribosomal subunit protein bL17 JW3256 b3294 protein protein ecoli Escherichia coli (strain K12) MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKELRRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGFRAGDNAPMAYIELVDRSEKAEAAAE DIP protein P0AG44 original sequence version: 71 DIP protein P0AG44 original sequence version: 46 D96DF17C07013A11 rl25_ecoli 50S ribosomal protein L25 rplY Large ribosomal subunit protein bL25 JW2173 b2185 protein protein ecoli Escherichia coli (strain K12) MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDHDKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA DIP protein P68919 original sequence version: 61 85DAF5ACBA1C5F86 rl2_ecoli 50S ribosomal protein L2 rplB Large ribosomal subunit protein uL2 JW3279 b3317 protein protein ecoli Escherichia coli (strain K12) MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRNNNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERRYILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGTYVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRPTVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK DIP protein P60422 original sequence version: 81 1010BDE8D8C68B9B rl9_ecoli 50S ribosomal protein L9 rplI Large ribosomal subunit protein bL9 JW4161 b4203 protein protein ecoli Escherichia coli (strain K12) MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNIEFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADAVTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE DIP protein P0A7R1 original sequence version: 58 DIP protein P0A7R1 original sequence version: 65 DIP protein P0A7R1 original sequence version: 69 F8FF527DBB9458CA rl1_ecoli 50S ribosomal protein L1 rplA Large ribosomal subunit protein uL1 JW3947 b3984 protein protein ecoli Escherichia coli (strain K12) MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDVAVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLADQIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQVRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTMGAGVAVDQAGLSASVN DIP protein P0A7L0 original sequence version: 52 DIP protein P0A7L0 original sequence version: 59 D751704B34748D53 rl21_ecoli 50S ribosomal protein L21 rplU Large ribosomal subunit protein bL21 JW3153 b3186 protein protein ecoli Escherichia coli (strain K12) MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANGEEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGISA DIP protein P0AG48 original sequence version: 44 9221F104EC9C8CDB rl6_ecoli 50S ribosomal protein L6 rplF Large ribosomal subunit protein uL6 JW3267 b3305 protein protein ecoli Escherichia coli (strain K12) MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVKHADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNVINLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGKGVRYADEVVRTKEAKKK DIP protein P0AG55 original sequence version: 48 946B64E9FA42FE61 rl27_ecoli 50S ribosomal protein L27 rpmA Large ribosomal subunit protein bL27 JW3152 b3185 protein protein ecoli Escherichia coli (strain K12) MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTKFHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE DIP protein P0A7L8 original sequence version: 55 B43AAE180ABC23EA frr-fusa-1 1 13 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 25 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 30 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 29 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 16 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 24 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 14 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 6 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 20 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 22 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 7 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 2 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 21 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 33 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 9 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 23 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 15 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 31 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 3 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 10 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 11 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 26 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 4 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 8 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 17 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 5 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 32 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 18 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 12 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 19 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 28 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 27 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified physical association physical association Fig 1