UniProt UniProt http://www.uniprot.org The heterodimeric structure of glucosidase II is required for its activity, solubility, and localization in vivo. The heterodimeric structure of glucosidase II is required for its activity, solubility, and localization in vivo. Glycobiology 2000 imex curation Pelletier MF., Marcil A., Sevigny G., Jakob CA., Tessier DC., Chevet E., Menard R., Bergeron JJ., Thomas DY., Pelletier MF., Marcil A., Sevigny G., Jakob CA., Tessier DC., Chevet E., Menard R., Bergeron JJ., Thomas DY. Only protein-protein interactions imex curation spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection chromatography chromatography technology column chromatography chromatography western blot western blot Immuno blot Immuno blot Pelletier MF., Marcil A., Sevigny G., Jakob CA., Tessier DC., Chevet E., Menard R., Bergeron JJ., Thomas DY., Pelletier MF., Marcil A., Sevigny G., Jakob CA., Tessier DC., Chevet E., Menard R., Bergeron JJ., Thomas DY. Infected cells were lysed in 1% Triton X100,complex purified on Ni2+-NTA resin, a Sephadex G-25M column, and a Mono QHR5/5 column. Accepted 2021-JUL-29 AT 08:22 BST AT 08:22 BST by LUANA Glycobiology 2000 Only protein-protein interactions imex curation imex curation The heterodimeric structure of glucosidase II is required for its activity, solubility, and localization in vivo. The heterodimeric structure of glucosidase II is required for its activity, solubility, and localization in vivo. Glycobiology 2000 imex curation Pelletier MF., Marcil A., Sevigny G., Jakob CA., Tessier DC., Chevet E., Menard R., Bergeron JJ., Thomas DY., Pelletier MF., Marcil A., Sevigny G., Jakob CA., Tessier DC., Chevet E., Menard R., Bergeron JJ., Thomas DY. Only protein-protein interactions imex curation in vitro In vitro cleavage cleavage assay cleavage predetermined predetermined participant predetermined Pelletier MF., Marcil A., Sevigny G., Jakob CA., Tessier DC., Chevet E., Menard R., Bergeron JJ., Thomas DY., Pelletier MF., Marcil A., Sevigny G., Jakob CA., Tessier DC., Chevet E., Menard R., Bergeron JJ., Thomas DY. Accepted 2021-JUL-29 AT 08:22 BST AT 08:22 BST by LUANA Glycobiology 2000 Only protein-protein interactions imex curation imex curation The heterodimeric structure of glucosidase II is required for its activity, solubility, and localization in vivo. The heterodimeric structure of glucosidase II is required for its activity, solubility, and localization in vivo. Glycobiology 2000 imex curation Pelletier MF., Marcil A., Sevigny G., Jakob CA., Tessier DC., Chevet E., Menard R., Bergeron JJ., Thomas DY., Pelletier MF., Marcil A., Sevigny G., Jakob CA., Tessier DC., Chevet E., Menard R., Bergeron JJ., Thomas DY. Only protein-protein interactions imex curation chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Pelletier MF., Marcil A., Sevigny G., Jakob CA., Tessier DC., Chevet E., Menard R., Bergeron JJ., Thomas DY., Pelletier MF., Marcil A., Sevigny G., Jakob CA., Tessier DC., Chevet E., Menard R., Bergeron JJ., Thomas DY. Infected cells were lysed in 1% Triton X100,complex purified on Ni2+-NTA resin, a Sephadex G-25M column, and a Mono QHR5/5 column. Accepted 2021-JUL-29 AT 08:22 BST AT 08:22 BST by LUANA Glycobiology 2000 Only protein-protein interactions imex curation imex curation q14697-1 Neutral alpha-glucosidase AB GANAB G2AN KIAA0088 Alpha-glucosidase 2 Glucosidase II subunit alpha protein protein human Homo sapiens Human MAAVAAVAARRRRSWASLVLAFLGVCLGITLAVDRSNFKTCEESSFCKRQRSIRPGLSPYRALLDSLQLGPDSLTVHLIHEVTKVLLVLELQGLQKNMTRFRIDELEPRRPRYRVPDVLVADPPIARLSVSGRDENSVELTMAEGPYKIILTARPFRLDLLEDRSLLLSVNARGLLEFEHQRAPRVSQGSKDPAEGDGAQPEETPRDGDKPEETQGKAEKDEPGAWEETFKTHSDSKPYGPMSVGLDFSLPGMEHVYGIPEHADNLRLKVTEGGEPYRLYNLDVFQYELYNPMALYGSVPVLLAHNPHRDLGIFWLNAAETWVDISSNTAGKTLFGKMMDYLQGSGETPQTDVRWMSETGIIDVFLLLGPSISDVFRQYASLTGTQALPPLFSLGYHQSRWNYRDEADVLEVDQGFDDHNLPCDVIWLDIEHADGKRYFTWDPSRFPQPRTMLERLASKRRKLVAIVDPHIKVDSGYRVHEELRNLGLYVKTRDGSDYEGWCWPGSAGYPDFTNPTMRAWWANMFSYDNYEGSAPNLFVWNDMNEPSVFNGPEVTMLKDAQHYGGWEHRDVHNIYGLYVHMATADGLRQRSGGMERPFVLARAFFAGSQRFGAVWTGDNTAEWDHLKISIPMCLSLGLVGLSFCGADVGGFFKNPEPELLVRWYQMGAYQPFFRAHAHLDTGRREPWLLPSQHNDIIRDALGQRYSLLPFWYTLLYQAHREGIPVMRPLWVQYPQDVTTFNIDDQYLLGDALLVHPVSDSGAHGVQVYLPGQGEVWYDIQSYQKHHGPQTLYLPVTLSSIPVFQRGGTIVPRWMRVRRSSECMKDDPITLFVALSPQGTAQGELFLDDGHTFNYQTRQEFLLRRFSFSGNTLVSSSADPEGHFETPIWIERVVIIGAGKPAAVVLQTKGSPESRLSFQHDPETSVLVLRKPGINVASDWSIHLR 9E3426FE9A016BF1 4-nitrophenyl alpha-d-glucoside 4-nitrophenyl alpha-D-glucoside 4-Nitrophenyl alpha-glucoside p-nitrophenyl alpha-D-glucoside 4-nitrophenyl alpha-D-glucopyranoside small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) high-mannose oligosaccharide high-mannose oligosaccharide high mannose oligosaccharide high mannose oligosaccharides high-mannose oligosaccharides small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) glu2b_human Glucosidase 2 subunit beta Glucosidase II subunit beta Protein kinase C substrate 60.1 kDa protein heavy chain 80K-H protein PRKCSH G19P1 protein protein human Homo sapiens Human MLLPLLLLLPMCWAVEVKRPRGVSLTNHHFYDESKPFTCLDGSATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYIPSNRVNDGVCDCCDGTDEYNSGVICENTCKEKGRKERESLQQMAEVTREGFRLKKILIEDWKKAREEKQKKLIELQAGKKSLEDQVEMLRTVKEEAEKPEREAKEQHQKLWEEQLAAAKAQQEQELAADAFKELDDDMDGTVSVTELQTHPELDTDGDGALSEAEAQALLSGDTQTDATSFYDRVWAAIRDKYRSEALPTDLPAPSAPDLTEPKEEQPPVPSSPTEEEEEEEEEEEEEAEEEEEEEDSEEAPPPLSPPQPASPAEEDKMPPYDEQTQAFIDAAQEARNKFEEAERSLKDMEESIRNLEQEISFDFGPNGEFAYLYSQCYELTTNEYVYRLCPFKLVSQKPKLGGSPTSLGTWGSWIGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETMVTSTTEPSRCEYLMELMTPAACPEPPPEAPTEDDHDEL 8DAD9776037E878E q14697-2 Neutral alpha-glucosidase AB Alpha-glucosidase 2 Glucosidase II subunit alpha GANAB G2AN KIAA0088 protein protein human Homo sapiens Human MAAVAAVAARRRRSWASLVLAFLGVCLGITLAVDRSNFKTCEESSFCKRQRSIRPGLSPYRALLDSLQLGPDSLTVHLIHEVTKVLLVLELQGLQKNMTRFRIDELEPRRPRYRVPDVLVADPPIARLSVSGRDENSVELTMAEGPYKIILTARPFRLDLLEDRSLLLSVNARGLLEFEHQRAPRVSFSDKVNLTLGSIWDKIKNLFSRQGSKDPAEGDGAQPEETPRDGDKPEETQGKAEKDEPGAWEETFKTHSDSKPYGPMSVGLDFSLPGMEHVYGIPEHADNLRLKVTEGGEPYRLYNLDVFQYELYNPMALYGSVPVLLAHNPHRDLGIFWLNAAETWVDISSNTAGKTLFGKMMDYLQGSGETPQTDVRWMSETGIIDVFLLLGPSISDVFRQYASLTGTQALPPLFSLGYHQSRWNYRDEADVLEVDQGFDDHNLPCDVIWLDIEHADGKRYFTWDPSRFPQPRTMLERLASKRRKLVAIVDPHIKVDSGYRVHEELRNLGLYVKTRDGSDYEGWCWPGSAGYPDFTNPTMRAWWANMFSYDNYEGSAPNLFVWNDMNEPSVFNGPEVTMLKDAQHYGGWEHRDVHNIYGLYVHMATADGLRQRSGGMERPFVLARAFFAGSQRFGAVWTGDNTAEWDHLKISIPMCLSLGLVGLSFCGADVGGFFKNPEPELLVRWYQMGAYQPFFRAHAHLDTGRREPWLLPSQHNDIIRDALGQRYSLLPFWYTLLYQAHREGIPVMRPLWVQYPQDVTTFNIDDQYLLGDALLVHPVSDSGAHGVQVYLPGQGEVWYDIQSYQKHHGPQTLYLPVTLSSIPVFQRGGTIVPRWMRVRRSSECMKDDPITLFVALSPQGTAQGELFLDDGHTFNYQTRQEFLLRRFSFSGNTLVSSSADPEGHFETPIWIERVVIIGAGKPAAVVLQTKGSPESRLSFQHDPETSVLVLRKPGINVASDWSIHLR D0CD9E47C8E88FB5 ganab-prkcsh-2 1 7 unspecified role unspecified role neutral component neutral component purified purified 4 unspecified role unspecified role neutral component neutral component purified purified c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range direct interaction direct interaction Fig 6A-D ganab-prkcsh-3 1 7 unspecified role unspecified role neutral component neutral component purified purified 8 unspecified role unspecified role neutral component neutral component purified purified c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range direct interaction direct interaction Fig 6A-D 4_nitrophenyl alpha_d_glucoside-ganab-2 2 5 enzyme target enzyme target substrate substrate neutral component neutral component 7 unspecified role unspecified role neutral component neutral component purified purified spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 4 enzyme enzyme neutral component neutral component purified purified c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection cleavage cleavage reaction cleavage Fig 6B, Table 1 4_nitrophenyl alpha_d_glucoside-ganab-1 2 5 enzyme target enzyme target substrate substrate neutral component neutral component 7 unspecified role unspecified role neutral component neutral component purified purified spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 8 enzyme enzyme neutral component neutral component purified purified c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection cleavage cleavage reaction cleavage Fig 6B, Table 1 ganab-prkcsh-5 3 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 4 ganab-high_mannose oligosaccharide-1 2 6 enzyme target enzyme target substrate substrate neutral component neutral component 7 unspecified role unspecified role neutral component neutral component purified purified spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 8 enzyme enzyme neutral component neutral component purified purified c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection cleavage cleavage reaction cleavage Fig 8 Cleaves Glc1Man9GlcNAc1, Glc2- and Glc1Man9GlcNAc1 but not Glc3Man9GlcNAc1 oligosaccharide ganab-high_mannose oligosaccharide-2 2 6 enzyme target enzyme target substrate substrate neutral component neutral component 7 unspecified role unspecified role neutral component neutral component purified purified spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 4 enzyme enzyme neutral component neutral component purified purified c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection cleavage cleavage reaction cleavage Fig 8 Cleaves Glc1Man9GlcNAc1, Glc2- and Glc1Man9GlcNAc1 but not Glc3Man9GlcNAc1 oligosaccharide