MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it The deubiquitinase USP44 promotes Treg function during inflammation by preventing FOXP3 degradation. The deubiquitinase USP44 promotes Treg function during inflammation by preventing FOXP3 degradation. EMBO reports 2020 imex curation Yang J., Wei P., Barbi J., Huang Q., Yang E., Bai Y., Nie J., Gao Y., Tao J., Lu Y., Xie C., Hou X., Ren J., Wu X., Meng J., Zhang Y., Fu J., Kou W., Gao Y., Chen Z., Liang R., Tsun A., Li D., Guo W., Zhang S., Zheng SG., Niu J., Galardy P., Tong X., Shi G., Li H., Pan F., Li B. sent email to author to request Ub gene and USP7, no answer allergyli@163.com; fan.pan@siat.ac.cn; binli@shsmu.edu.cn 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-jurkat Homo sapiens jurkat Human T cell leukemia confocal microscopy confocal microscopy immunostaining immunostaining Yang J., Wei P., Barbi J., Huang Q., Yang E., Bai Y., Nie J., Gao Y., Tao J., Lu Y., Xie C., Hou X., Ren J., Wu X., Meng J., Zhang Y., Fu J., Kou W., Gao Y., Chen Z., Liang R., Tsun A., Li D., Guo W., Zhang S., Zheng SG., Niu J., Galardy P., Tong X., Shi G., Li H., Pan F., Li B. sent email to author to request Ub gene and USP7, no answer allergyli@163.com; fan.pan@siat.ac.cn; binli@shsmu.edu.cn Accepted 2020-OCT-13 AT 09:06 BST AT 09:06 BST by LIVIAP EMBO reports 2020 Only protein-protein interactions imex curation imex curation The deubiquitinase USP44 promotes Treg function during inflammation by preventing FOXP3 degradation. The deubiquitinase USP44 promotes Treg function during inflammation by preventing FOXP3 degradation. EMBO reports 2020 imex curation Yang J., Wei P., Barbi J., Huang Q., Yang E., Bai Y., Nie J., Gao Y., Tao J., Lu Y., Xie C., Hou X., Ren J., Wu X., Meng J., Zhang Y., Fu J., Kou W., Gao Y., Chen Z., Liang R., Tsun A., Li D., Guo W., Zhang S., Zheng SG., Niu J., Galardy P., Tong X., Shi G., Li H., Pan F., Li B. sent email to author to request Ub gene and USP7, no answer allergyli@163.com; fan.pan@siat.ac.cn; binli@shsmu.edu.cn 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-t-cell Mouse T cell t-cell Peripheral blood T-lymphocytes anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yang J., Wei P., Barbi J., Huang Q., Yang E., Bai Y., Nie J., Gao Y., Tao J., Lu Y., Xie C., Hou X., Ren J., Wu X., Meng J., Zhang Y., Fu J., Kou W., Gao Y., Chen Z., Liang R., Tsun A., Li D., Guo W., Zhang S., Zheng SG., Niu J., Galardy P., Tong X., Shi G., Li H., Pan F., Li B. nTregs (CD4+CD25high) isolated by FACS were activated by anti-CD3 and anti-CD28 (1 and 4 lg/ml,respectively) overnight in the presence of IL-2 (100 U/ml). Tregs were generated as in Fig 1 from naïve CD4+T cells FACS isolated from pooled suspensions of thelymph node and spleen cells of wild-type C57BL/6 mice (n = 2–3/experiment). sent email to author to request Ub gene and USP7, no answer allergyli@163.com; fan.pan@siat.ac.cn; binli@shsmu.edu.cn Accepted 2020-OCT-13 AT 09:06 BST AT 09:06 BST by LIVIAP EMBO reports 2020 Only protein-protein interactions imex curation imex curation The deubiquitinase USP44 promotes Treg function during inflammation by preventing FOXP3 degradation. The deubiquitinase USP44 promotes Treg function during inflammation by preventing FOXP3 degradation. EMBO reports 2020 imex curation Yang J., Wei P., Barbi J., Huang Q., Yang E., Bai Y., Nie J., Gao Y., Tao J., Lu Y., Xie C., Hou X., Ren J., Wu X., Meng J., Zhang Y., Fu J., Kou W., Gao Y., Chen Z., Liang R., Tsun A., Li D., Guo W., Zhang S., Zheng SG., Niu J., Galardy P., Tong X., Shi G., Li H., Pan F., Li B. sent email to author to request Ub gene and USP7, no answer allergyli@163.com; fan.pan@siat.ac.cn; binli@shsmu.edu.cn 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-t-cell Mouse T cell t-cell Peripheral blood T-lymphocytes confocal microscopy confocal microscopy immunostaining immunostaining Yang J., Wei P., Barbi J., Huang Q., Yang E., Bai Y., Nie J., Gao Y., Tao J., Lu Y., Xie C., Hou X., Ren J., Wu X., Meng J., Zhang Y., Fu J., Kou W., Gao Y., Chen Z., Liang R., Tsun A., Li D., Guo W., Zhang S., Zheng SG., Niu J., Galardy P., Tong X., Shi G., Li H., Pan F., Li B. sent email to author to request Ub gene and USP7, no answer allergyli@163.com; fan.pan@siat.ac.cn; binli@shsmu.edu.cn Accepted 2020-OCT-13 AT 09:06 BST AT 09:06 BST by LIVIAP EMBO reports 2020 Only protein-protein interactions imex curation imex curation The deubiquitinase USP44 promotes Treg function during inflammation by preventing FOXP3 degradation. The deubiquitinase USP44 promotes Treg function during inflammation by preventing FOXP3 degradation. EMBO reports 2020 imex curation Yang J., Wei P., Barbi J., Huang Q., Yang E., Bai Y., Nie J., Gao Y., Tao J., Lu Y., Xie C., Hou X., Ren J., Wu X., Meng J., Zhang Y., Fu J., Kou W., Gao Y., Chen Z., Liang R., Tsun A., Li D., Guo W., Zhang S., Zheng SG., Niu J., Galardy P., Tong X., Shi G., Li H., Pan F., Li B. sent email to author to request Ub gene and USP7, no answer allergyli@163.com; fan.pan@siat.ac.cn; binli@shsmu.edu.cn 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yang J., Wei P., Barbi J., Huang Q., Yang E., Bai Y., Nie J., Gao Y., Tao J., Lu Y., Xie C., Hou X., Ren J., Wu X., Meng J., Zhang Y., Fu J., Kou W., Gao Y., Chen Z., Liang R., Tsun A., Li D., Guo W., Zhang S., Zheng SG., Niu J., Galardy P., Tong X., Shi G., Li H., Pan F., Li B. sent email to author to request Ub gene and USP7, no answer allergyli@163.com; fan.pan@siat.ac.cn; binli@shsmu.edu.cn Accepted 2020-OCT-13 AT 09:06 BST AT 09:06 BST by LIVIAP EMBO reports 2020 Only protein-protein interactions imex curation imex curation ubp44_human Ubiquitin carboxyl-terminal hydrolase 44 USP44 Ubiquitin-specific-processing protease 44 Deubiquitinating enzyme 44 Ubiquitin thioesterase 44 protein protein human Homo sapiens Human MLAMDTCKHVGQLQLAQDHSSLNPQKWHCVDCNTTESIWACLSCSHVACGRYIEEHALKHFQESSHPVALEVNEMYVFCYLCDDYVLNDNTTGDLKLLRRTLSAIKSQNYHCTTRSGRFLRSMGTGDDSYFLHDGAQSLLQSEDQLYTALWHRRRILMGKIFRTWFEQSPIGRKKQEEPFQEKIVVKREVKKRRQELEYQVKAELESMPPRKSLRLQGLAQSTIIEIVSVQVPAQTPASPAKDKVLSTSENEISQKVSDSSVKRRPIVTPGVTGLRNLGNTCYMNSVLQVLSHLLIFRQCFLKLDLNQWLAMTASEKTRSCKHPPVTDTVVYQMNECQEKDTGFVCSRQSSLSSGLSGGASKGRKMELIQPKEPTSQYISLCHELHTLFQVMWSGKWALVSPFAMLHSVWRLIPAFRGYAQQDAQEFLCELLDKIQRELETTGTSLPALIPTSQRKLIKQVLNVVNNIFHGQLLSQVTCLACDNKSNTIEPFWDLSLEFPERYQCSGKDIASQPCLVTEMLAKFTETEALEGKIYVCDQCNSKRRRFSSKPVVLTEAQKQLMICHLPQVLRLHLKRFRWSGRNNREKIGVHVGFEEILNMEPYCCRETLKSLRPECFIYDLSAVVMHHGKGFGSGHYTAYCYNSEGGFWVHCNDSKLSMCTMDEVCKAQAYILFYTQRVTENGHSKLLPPELLLGSQHPNEDADTSSNEILS A8211DFAD78EE731 foxp3_human Forkhead box protein P3 Scurfin FOXP3 IPEX JM2 protein protein human Homo sapiens Human MPNPRPGKPSAPSLALGPSPGASPSWRAAPKASDLLGARGPGGTFQGRDLRGGAHASSSSLNPMPPSQLQLPTLPLVMVAPSGARLGPLPHLQALLQDRPHFMHQLSTVDAHARTPVLQVHPLESPAMISLTPPTTATGVFSLKARPGLPPGINVASLEWVSREPALLCTFPNPSAPRKDSTLSAVPQSSYPLLANGVCKWPGCEKVFEEPEDFLKHCQADHLLDEKGRAQCLLQREMVQSLEQQLVLEKEKLSAMQAHLAGKMALTKASSVASSDKGSCCIVAAGSQGPVVPAWSGPREAPDSLFAVRRHLWGSHGNSTFPEFLHNMDYFKFHNMRPPFTYATLIRWAILEAPEKQRTLNEIYHWFTRMFAFFRNHPATWKNAIRHNLSLHKCFVRVESEKGAVWTVDELEFRKKRSQRPSRCSNPTPGP DIP protein Q9BZS1 original sequence version: 139 DIP protein Q9BZS1 original sequence version: 131 91737C3CEA665A15 foxp3_mouse Forkhead box protein P3 Foxp3 Scurfin protein protein mouse Mus musculus Mouse MPNPRPAKPMAPSLALGPSPGVLPSWKTAPKGSELLGTRGSGGPFQGRDLRSGAHTSSSLNPLPPSQLQLPTVPLVMVAPSGARLGPSPHLQALLQDRPHFMHQLSTVDAHAQTPVLQVRPLDNPAMISLPPPSAATGVFSLKARPGLPPGINVASLEWVSREPALLCTFPRSGTPRKDSNLLAAPQGSYPLLANGVCKWPGCEKVFEEPEEFLKHCQADHLLDEKGKAQCLLQREVVQSLEQQLELEKEKLGAMQAHLAGKMALAKAPSVASMDKSSCCIVATSTQGSVLPAWSAPREAPDGGLFAVRRHLWGSHGNSSFPEFFHNMDYFKYHNMRPPFTYATLIRWAILEAPERQRTLNEIYHWFTRMFAYFRNHPATWKNAIRHNLSLHKCFVRVESEKGAVWTVDEFEFRKKRSQRPNKCSNPCP DIP protein Q99JB6 original sequence version: 120 DIP protein Q99JB6 original sequence version: 110 DIP protein Q99JB6 original sequence version: 114 DIP protein Q99JB6 original sequence version: 102 DIP protein Q99JB6 original sequence version: 100 28D5B8E67891840C ubp44_mouse Ubiquitin carboxyl-terminal hydrolase 44 Deubiquitinating enzyme 44 Ubiquitin thioesterase 44 Ubiquitin-specific-processing protease 44 Usp44 protein protein mouse Mus musculus Mouse MDRCKHVEQLQLAQGHSILDPQKWYCMVCNTTESIWACLSCSHVACGKYIQEHALKHFQESSHPVAFEVNDMYAFCYLCNDYVLNDNAAGDLKSLRSTLSTIKSKKYPCVVPSDSVLHPVDAQDRVYSLLDGTQSLPGNEDPTCAALWHRRRVLMGKAFRTWFEQSAIGRKGQEPTQERMVAKREAKRRQQQELEQQMKAELESTPPRKSLRLQGSSEEAATIEIVPVRAPPPPPASPAKDKAALPTSEDRTFKKVSDSLIKRRPMVTPGVTGLRNLGNTCYMNSVLQVLSHLLIFRQCFLKLDLNQWLAVAASDKARSYKHSAVTEAAAQQMNEGQEKEKGFVCSRHSGLSSGLSGGASKGRNMELIQPREPSSPYSSLCHELHILFQVMWSGEWALVSPFAMLHSVWRLIPAFRGYAQQDAQEFLCELLDKIQRELETTGTKLPALIPTSQRRLIEQVLNVVNNIFHGQFLSQVTCLACDNKSDTIESFWDLSLEFPERYQCSGKDAASQPCLVTDMLDKFTETEALEGKIYMCDHCNSKRRKFSSKSVVFTEAQKQLMICHLPQVLRLHLKRFRWSGRNNREKIGVHVVFEETLNMEPYCCRETLNALRPECFLYNLSAVVIHHGKGFGSGHYTAYCYNSEGGFWVHCNDSKLSMCTMEEVRKAQAYILFYTQRVTENGHSKLLPPELLSNSQHPSKETDASSNEVLS F176DB2BA3C658BE foxp3-usp44-1 4 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association fig.2a left foxp3-usp44-2 4 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association fig.2a right foxp3-usp44-4 2 7 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association fig.2b-c (left) Specifically, immunoprecipitation ofFOXP3 from the lysate of murine iTregs or nTregs readily pulleddown USP44, and this DUB was similarly able to coimmunoprecipi-tate FOXP3 in reciprocal experiments (Fig 2B and C). foxp3-usp44-3 2 7 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association fig.2b-c (right) Specifically, immunoprecipitation ofFOXP3 from the lysate of murine iTregs or nTregs readily pulleddown USP44, and this DUB was similarly able to coimmunoprecipi-tate FOXP3 in reciprocal experiments (Fig 2B and C). 1-164 3 8 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell 7 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell colocalization colocalization fig. 2d Confocal microscopy of primary murine Tregs confirmed the colocalization of FOXP3 and USP44 in wild‐type‐derived cells but not in cells isolated from mice lacking USP44 expression (Usp44fl/flFoxp3Cre+; Fig 2D). 1-165 1 5 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization EV4