DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure of human GABAB receptor in an inactive state. Structure of human GABAB receptor in an inactive state. Nature 2020 imex curation Park J., Fu Z., Frangaj A., Liu J., Mosyak L., Shen T., Slavkovich VN., Ray KM., Taura J., Cao B., Geng Y., Zuo H., Kou Y., Grassucci R., Chen S., Liu Z., Lin X., Williams JP., Rice WJ., Eng ET., Huang RK., Soni RK., Kloss B., Yu Z., Javitch JA., Hendrickson WA., Slesinger PA., Quick M., Graziano J., Yu H., Fiehn O., Clarke OB., Frank J., Fan QR. oc2188@cumc.columbia.edu, jf2192@cumc.columbia.edu, qf13@cumc.columbia.edu 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient Human 293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient ATCC® CRL-3022 molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Park J., Fu Z., Frangaj A., Liu J., Mosyak L., Shen T., Slavkovich VN., Ray KM., Taura J., Cao B., Geng Y., Zuo H., Kou Y., Grassucci R., Chen S., Liu Z., Lin X., Williams JP., Rice WJ., Eng ET., Huang RK., Soni RK., Kloss B., Yu Z., Javitch JA., Hendrickson WA., Slesinger PA., Quick M., Graziano J., Yu H., Fiehn O., Clarke OB., Frank J., Fan QR. oc2188@cumc.columbia.edu, jf2192@cumc.columbia.edu, qf13@cumc.columbia.edu Accepted 2020-JUL-21 AT 12:02 BST AT 12:02 BST by MGT Nature 2020 Only protein-protein interactions imex curation imex curation Structure of human GABAB receptor in an inactive state. Structure of human GABAB receptor in an inactive state. Nature 2020 imex curation Park J., Fu Z., Frangaj A., Liu J., Mosyak L., Shen T., Slavkovich VN., Ray KM., Taura J., Cao B., Geng Y., Zuo H., Kou Y., Grassucci R., Chen S., Liu Z., Lin X., Williams JP., Rice WJ., Eng ET., Huang RK., Soni RK., Kloss B., Yu Z., Javitch JA., Hendrickson WA., Slesinger PA., Quick M., Graziano J., Yu H., Fiehn O., Clarke OB., Frank J., Fan QR. oc2188@cumc.columbia.edu, jf2192@cumc.columbia.edu, qf13@cumc.columbia.edu 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient Human 293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient ATCC® CRL-3022 3d-em-single electron microscopy 3D single particle reconstruction 3D-EM-single predetermined predetermined participant predetermined Park J., Fu Z., Frangaj A., Liu J., Mosyak L., Shen T., Slavkovich VN., Ray KM., Taura J., Cao B., Geng Y., Zuo H., Kou Y., Grassucci R., Chen S., Liu Z., Lin X., Williams JP., Rice WJ., Eng ET., Huang RK., Soni RK., Kloss B., Yu Z., Javitch JA., Hendrickson WA., Slesinger PA., Quick M., Graziano J., Yu H., Fiehn O., Clarke OB., Frank J., Fan QR. oc2188@cumc.columbia.edu, jf2192@cumc.columbia.edu, qf13@cumc.columbia.edu Accepted 2020-JUL-21 AT 12:02 BST AT 12:02 BST by MGT Nature 2020 Only protein-protein interactions imex curation imex curation q9ubs5-2 Gamma-aminobutyric acid type B receptor subunit 1 GABBR1 GPRC3A protein protein human Homo sapiens Human MGPGAPFARVGWPLPLLVVMAAGVAPVWASHSPHLPRPHSRVPPHPSSERRAVYIGALFPMSGGWPGGQACQPAVEMALEDVNSRRDILPDYELKLIHHDSKCDPGQATKYLYELLYNDPIKIILMPGCSSVSTLVAEAARMWNLIVLSYGSSSPALSNRQRFPTFFRTHPSATLHNPTRVKLFEKWGWKKIATIQQTTEVFTSTLDDLEERVKEAGIEITFRQSFFSDPAVPVKNLKRQDARIIVGLFYETEARKVFCEVYKERLFGKKYVWFLIGWYADNWFKIYDPSINCTVDEMTEAVEGHITTEIVMLNPANTRSISNMTSQEFVEKLTKRLKRHPEETGGFQEAPLAYDAIWALALALNKTSGGGGRSGVRLEDFNYNNQTITDQIYRAMNSSSFEGVSGHVVFDASGSRMAWTLIEQLQGGSYKKIGYYDSTKDDLSWSKTDKWIGGSPPADQTLVIKTFRFLSQKLFISVSVLSSLGIVLAVVCLSFNIYNSHVRYIQNSQPNLNNLTAVGCSLALAAVFPLGLDGYHIGRNQFPFVCQARLWLLGLGFSLGYGSMFTKIWWVHTVFTKKEEKKEWRKTLEPWKLYATVGLLVGMDVLTLAIWQIVDPLHRTIETFAKEEPKEDIDVSILPQLEHCSSRKMNTWLGIFYGYKGLLLLLGIFLAYETKSVSTEKINDHRAVGMAIYNVAVLCLITAPVTMILSSQQDAAFAFASLAIVFSSYITLVVLFVPKMRRLITRGEWQSEAQDTMKTGSSTNNNEEEKSRLLEKENRELEKIIAEKEERVSELRHQLQSRQQLRSRRHPPTPPEPSGGLPRGPPEPPDRLSCDGSRVHLLYK DIP protein Q9UBS5-2 original sequence version: 140 gabr2_human Gamma-aminobutyric acid type B receptor subunit 2 G-protein coupled receptor 51 HG20 GABBR2 GPR51 GPRC3B protein protein human Homo sapiens Human MASPRSSGQPGPPPPPPPPPARLLLLLLLPLLLPLAPGAWGWARGAPRPPPSSPPLSIMGLMPLTKEVAKGSIGRGVLPAVELAIEQIRNESLLRPYFLDLRLYDTECDNAKGLKAFYDAIKYGPNHLMVFGGVCPSVTSIIAESLQGWNLVQLSFAATTPVLADKKKYPYFFRTVPSDNAVNPAILKLLKHYQWKRVGTLTQDVQRFSEVRNDLTGVLYGEDIEISDTESFSNDPCTSVKKLKGNDVRIILGQFDQNMAAKVFCCAYEENMYGSKYQWIIPGWYEPSWWEQVHTEANSSRCLRKNLLAAMEGYIGVDFEPLSSKQIKTISGKTPQQYEREYNNKRSGVGPSKFHGYAYDGIWVIAKTLQRAMETLHASSRHQRIQDFNYTDHTLGRIILNAMNETNFFGVTGQVVFRNGERMGTIKFTQFQDSREVKVGEYNAVADTLEIINDTIRFQGSEPPKDKTIILEQLRKISLPLYSILSALTILGMIMASAFLFFNIKNRNQKLIKMSSPYMNNLIILGGMLSYASIFLFGLDGSFVSEKTFETLCTVRTWILTVGYTTAFGAMFAKTWRVHAIFKNVKMKKKIIKDQKLLVIVGGMLLIDLCILICWQAVDPLRRTVEKYSMEPDPAGRDISIRPLLEHCENTHMTIWLGIVYAYKGLLMLFGCFLAWETRNVSIPALNDSKYIGMSVYNVGIMCIIGAAVSFLTRDQPNVQFCIVALVIIFCSTITLCLVFVPKLITLRTNPDAATQNRRFQFTQNQKKEDSKTSTSVTSVNQASTSRLEGLQSENHRLRMKITELDKDLEEVTMQLQDTPEKTTYIKQNHYQELNDILNLGNFTESTDGGKAILKNHLDQNPQLQWNTTEPSRTCKDPIEDINSPEHIQRRLSLQLPILHHAYLPSIGGVDASCVSPCVSPTASPRHRHVPPSFRVMVSGL DIP protein O75899 original sequence version: 136 09F1773DB0673C5D gabbr1-gabbr2-12 2 4 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified 1-819 binding region 1-819 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain FLAG Tag FLAG Tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient Human 293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient ATCC® CRL-3022 3 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified 1-802 binding region 1-802 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain FLAG Tag FLAG Tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient Human 293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient ATCC® CRL-3022 direct interaction direct interaction Fig 1, Sup. Table 1 gabbr1-gabbr2-13 1 4 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed 1-819 binding region 1-819 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain FLAG Tag FLAG Tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient Human 293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient ATCC® CRL-3022 3 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed 1-802 binding region 1-802 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain FLAG Tag FLAG Tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient Human 293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient ATCC® CRL-3022 direct interaction direct interaction Ext Data Fig 1