IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ LncRNA NEAT1/miR-185-5p/IGF2 axis regulates the invasion and migration of colon cancer. LncRNA NEAT1/miR-185-5p/IGF2 axis regulates the invasion and migration of colon cancer. Molecular genetics & genomic medicine 2020 imex curation Zhuang ST., Cai YJ., Liu HP., Qin Y., Wen JF. szcaiyanjuan@163.com 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-sw480 Human SW480 adenocarcinoma cell line sw480 SW480- human primary colorectal adenocarcinoma cell line There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution and a C -> T mutation in codon 309 resulting in a Pro -> Ser substitution. http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CCL-228&Template=cellBiology mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag luciferase tag visualisation by luciferase assay tag luciferase Zhuang ST., Cai YJ., Liu HP., Qin Y., Wen JF. szcaiyanjuan@163.com Accepted 2020-OCT-26 AT 13:31 GMT AT 13:31 GMT by MGT Molecular genetics & genomic medicine 2020 Only protein-protein interactions imex curation imex curation LncRNA NEAT1/miR-185-5p/IGF2 axis regulates the invasion and migration of colon cancer. LncRNA NEAT1/miR-185-5p/IGF2 axis regulates the invasion and migration of colon cancer. Molecular genetics & genomic medicine 2020 imex curation Zhuang ST., Cai YJ., Liu HP., Qin Y., Wen JF. szcaiyanjuan@163.com 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-ht_29 Homo sapiens HT-29 caucasian women with colon adenocarcinoma ht_29 HT-29 confirmed as human with IEF of AST, LDH established from the primary tumor of a 44-year-old Caucasian woman with colon adenocarcinoma in 1964; described to be heterotransplantable forming well-differentiated grade I tumors rna-ip rna immunoprecipitation rna-ip qrt-pcr quantitative reverse transcription pcr QRT-PCR Zhuang ST., Cai YJ., Liu HP., Qin Y., Wen JF. szcaiyanjuan@163.com Accepted 2020-OCT-26 AT 13:31 GMT AT 13:31 GMT by MGT Molecular genetics & genomic medicine 2020 Only protein-protein interactions imex curation imex curation LncRNA NEAT1/miR-185-5p/IGF2 axis regulates the invasion and migration of colon cancer. LncRNA NEAT1/miR-185-5p/IGF2 axis regulates the invasion and migration of colon cancer. Molecular genetics & genomic medicine 2020 imex curation Zhuang ST., Cai YJ., Liu HP., Qin Y., Wen JF. szcaiyanjuan@163.com 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-sw480 Human SW480 adenocarcinoma cell line sw480 SW480- human primary colorectal adenocarcinoma cell line There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution and a C -> T mutation in codon 309 resulting in a Pro -> Ser substitution. http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CCL-228&Template=cellBiology rna-ip rna immunoprecipitation rna-ip qrt-pcr quantitative reverse transcription pcr QRT-PCR Zhuang ST., Cai YJ., Liu HP., Qin Y., Wen JF. szcaiyanjuan@163.com Accepted 2020-OCT-26 AT 13:31 GMT AT 13:31 GMT by MGT Molecular genetics & genomic medicine 2020 Only protein-protein interactions imex curation imex curation LncRNA NEAT1/miR-185-5p/IGF2 axis regulates the invasion and migration of colon cancer. LncRNA NEAT1/miR-185-5p/IGF2 axis regulates the invasion and migration of colon cancer. Molecular genetics & genomic medicine 2020 imex curation Zhuang ST., Cai YJ., Liu HP., Qin Y., Wen JF. szcaiyanjuan@163.com 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-ht_29 Homo sapiens HT-29 caucasian women with colon adenocarcinoma ht_29 HT-29 confirmed as human with IEF of AST, LDH established from the primary tumor of a 44-year-old Caucasian woman with colon adenocarcinoma in 1964; described to be heterotransplantable forming well-differentiated grade I tumors mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag luciferase tag visualisation by luciferase assay tag luciferase Zhuang ST., Cai YJ., Liu HP., Qin Y., Wen JF. szcaiyanjuan@163.com Accepted 2020-OCT-26 AT 13:31 GMT AT 13:31 GMT by MGT Molecular genetics & genomic medicine 2020 Only protein-protein interactions imex curation imex curation LncRNA NEAT1/miR-185-5p/IGF2 axis regulates the invasion and migration of colon cancer. LncRNA NEAT1/miR-185-5p/IGF2 axis regulates the invasion and migration of colon cancer. Molecular genetics & genomic medicine 2020 imex curation Zhuang ST., Cai YJ., Liu HP., Qin Y., Wen JF. szcaiyanjuan@163.com 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-ht_29 Homo sapiens HT-29 caucasian women with colon adenocarcinoma ht_29 HT-29 confirmed as human with IEF of AST, LDH established from the primary tumor of a 44-year-old Caucasian woman with colon adenocarcinoma in 1964; described to be heterotransplantable forming well-differentiated grade I tumors pull down pull down qrt-pcr quantitative reverse transcription pcr QRT-PCR Zhuang ST., Cai YJ., Liu HP., Qin Y., Wen JF. szcaiyanjuan@163.com Accepted 2020-OCT-26 AT 13:31 GMT AT 13:31 GMT by MGT Molecular genetics & genomic medicine 2020 Only protein-protein interactions imex curation imex curation mrna_igf2 long Homo sapiens insulin like growth factor 2 (IGF2), transcript variant 1 mrna messenger rna mRNA mrna human Homo sapiens Human GCCTGCTCTCCGGCGGAGCTGCGTGAGGCCCGGCCGGCCCCGGCCCCCCCCTTCCGGCCGCCCCCGCCTCCTGGCCCACGCCTGCCCGCGCTCTGCCCACCAGCGCCTCCATCGGGCAAGGCGGCCCCGCGTCGACGCCGCCCGCTGCCTCGCTGCTGACTCCCGTCCCGGGCGCCGTCCGCGGGGTCGCGCTCCGCCGGGCCTGCGGATTCCCCGCCGCCTCCTCTTCATCTACCTCAACTCCCCCCATCCCCGCTTCGCCCGAGGAGGCGGTTCCCCCCGCAGGCAGTCCGGCTCGCAGGCCGCCGGCGTTGTCACCCCCCCCCGCGCTCCCCCTCCAGCCCTCCCCCCGGCGCGCAGCCTCGGGCCGCTCCCCTTTCCGCGCTGCGTCCCGGAGCGGCCCCGGTGCCGCCACCGCCTGTCCCCCTCCCGAGGCCCGGGCTCGCGACGGCAGAGGGCTCCGTCGGCCCAAACCGAGCTGGGCGCCCGCGGTCCGGGTGCAGCCTCCACTCCGCCCCCCAGTCACCGCCTCCCCCGGCCCCTCGACGTGGCGCCCTTCCCTCCGCTTCTCTGTGCTCCCCGCGCCCCTCTTGGCGTCTGGCCCCGGCCCCCGCTCTTTCTCCCGCAACCTTCCCTTCGCTCCCTCCCGTCCCCCCCAGCTCCTAGCCTCCGACTCCCTCCCCCCCTCACGCCCGCCCTCTCGCCTTCGCCGAACCAAAGTGGATTAATTACACGCTTTCTGTTTCTCTCCGTGCTGTTCTCTCCCGCTGTGCGCCTGCCCGCCTCTCGCTGTCCTCTCTCCCCCTCGCCCTCTCTTCGGCCCCCCCCTTTCACGTTCACTCTGTCTCTCCCACTATCTCTGCCCCCCTCTATCCTTGATACAACAGCTGACCTCATTTCCCGATACCTTTTCCCCCCCGAAAAGTACAACATCTGGCCCGCCCCAGCCCGAAGACAGCCCGTCCTCCCTGGACAATCAGACGAATTCTCCCCCCCCCCCCAAAAAAAAGCCATCCCCCCGCTCTGCCCCGTCGCACATTCGGCCCCCGCGACTCGGCCAGAGCGGCGCTGGCAGAGGAGTGTCCGGCAGGAGGGCCAACGCCCGCTGTTCGGTTTGCGACACGCAGCAGGGAGGTGGGCGGCAGCGTCGCCGGCTTCCAGACACCAATGGGAATCCCAATGGGGAAGTCGATGCTGGTGCTTCTCACCTTCTTGGCCTTCGCCTCGTGCTGCATTGCTGCTTACCGCCCCAGTGAGACCCTGTGCGGCGGGGAGCTGGTGGACACCCTCCAGTTCGTCTGTGGGGACCGCGGCTTCTACTTCAGCAGGCCCGCAAGCCGTGTGAGCCGTCGCAGCCGTGGCATCGTTGAGGAGTGCTGTTTCCGCAGCTGTGACCTGGCCCTCCTGGAGACGTACTGTGCTACCCCCGCCAAGTCCGAGAGGGACGTGTCGACCCCTCCGACCGTGCTTCCGGACAACTTCCCCAGATACCCCGTGGGCAAGTTCTTCCAATATGACACCTGGAAGCAGTCCACCCAGCGCCTGCGCAGGGGCCTGCCTGCCCTCCTGCGTGCCCGCCGGGGTCACGTGCTCGCCAAGGAGCTCGAGGCGTTCAGGGAGGCCAAACGTCACCGTCCCCTGATTGCTCTACCCACCCAAGACCCCGCCCACGGGGGCGCCCCCCCAGAGATGGCCAGCAATCGGAAGTGAGCAAAACTGCCGCAAGTCTGCAGCCCGGCGCCACCATCCTGCAGCCTCCTCCTGACCACGGACGTTTCCATCAGGTTCCATCCCGAAAATCTCTCGGTTCCACGTCCCCCTGGGGCTTCTCCTGACCCAGTCCCCGTGCCCCGCCTCCCCGAAACAGGCTACTCTCCTCGGCCCCCTCCATCGGGCTGAGGAAGCACAGCAGCATCTTCAAACATGTACAAAATCGATTGGCTTTAAACACCCTTCACATACCCTCCCCCCAAATTATCCCCAATTATCCCCACACATAAAAAATCAAAACATTAAACTAACCCCCTTCCCCCCCCCCCACAACAACCCTCTTAAAACTAATTGGCTTTTTAGAAACACCCCACAAAAGCTCAGAAATTGGCTTTAAAAAAAACAACCACCAAAAAAAATCAATTGGCTAAAAAAAAAAAGTATTAAAAACGAATTGGCTGAGAAACAATTGGCAAAATAAAGGAATTTGGCACTCCCCACCCCCCTCTTTCTCTTCTCCCTTGGACTTTGAGTCAAATTGGCCTGGACTTGAGTCCCTGAACCAGCAAAGAGAAAAGAAGGACCCCAGAAATCACAGGTGGGCACGTCGCTGCTACCGCCATCTCCCTTCTCACGGGAATTTTCAGGGTAAACTGGCCATCCGAAAATAGCAACAACCCAGACTGGCTCCTCACTCCCTTTTCCATCACTAAAAATCACAGAGCAGTCAGAGGGACCCAGTAAGACCAAAGGAGGGGAGGACAGAGCATGAAAACCAAAATCCATGCAAATGAAATGTAATTGGCACGACCCTCACCCCCAAATCTTACATCTCAATTCCCATCCTAAAAAGCACTCATACTTTATGCATCCCCGCAGCTACACACACACAACACACAGCACACGCATGAACACAGCACACACACGAGCACAGCACACACACAAACGCACAGCACACACAGCACACAGATGAGCACACAGCACACACACAAACGCACAGCACACACACGCACACACATGCACACACAGCACACAAACGCACGGCACACACACGCACACACATGCACACACAGCACACACACAAACGCACAGCACACACAAACGCACAGCACACACGCACACACAGCACACACACGAGCACACAGCACACAAACGCACAGCACACGCACACACATGCACACACAGCACACACACTAGCACACAGCACACACACAAAGACACAGCACACACATGCACACACAGCACACACACGCGAACACAGCACACACGAACACAGCACACACAGCACACACACAAACACAGCACACACATGCACACAGCACACGCACACACAGCACACACATGAACACAGCACACAGCACACACATGCACACACAGCACACACGCATGCACAGCACACATGAACACAGCACACACACAAACACACAGCACACACATGCACACACAGCACACACACTCATGCGCAGCACATACATGAACACAGCTCACAGCACACAAACACGCAGCACACACGTTGCACACGCAAGCACCCACCTGCACACACACATGCGCACACACACGCACACCCCCACAAAATTGGATGAAAACAATAAGCATATCTAAGCAACTACGATATCTGTATGGATCAGGCCAAAGTCCCGCTAAGATTCTCCAATGTTTTCATGGTCTGAGCCCCGCTCCTGTTCCCATCTCCACTGCCCCTCGGCCCTGTCTGTGCCCTGCCTCTCAGAGGAGGGGGCTCAGATGGTGCGGCCTGAGTGTGCGGCCGGCGGCATTTGGGATACACCCGTAGGGTGGGCGGGGTGTGTCCCAGGCCTAATTCCATCTTTCCACCATGACAGAGATGCCCTTGTGAGGCTGGCCTCCTTGGCGCCTGTCCCCACGGCCCCCGCAGCGTGAGCCACGATGCTCCCCATACCCCACCCATTCCCGATACACCTTACTTACTGTGTGTTGGCCCAGCCAGAGTGAGGAAGGAGTTTGGCCACATTGGAGATGGCGGTAGCTGAGCAGACATGCCCCCACGAGTAGCCTGACTCCCTGGTGTGCTCCTGGAAGGAAGATCTTGGGGACCCCCCCACCGGAGCACACCTAGGGATCATCTTTGCCCGTCTCCTGGGGACCCCCCAAGAAATGTGGAGTCCTCGGGGGCCGTGCACTGATGCGGGGAGTGTGGGAAGTCTGGCGGTTGGAGGGGTGGGTGGGGGGCAGTGGGGGCTGGGCGGGGGGAGTTCTGGGGTAGGAAGTGGTCCCGGGAGATTTTGGATGGAAAAGTCAGGAGGATTGACAGCAGACTTGCAGAATTACATAGAGAAATTAGGAACCCCCAAATTTCATGTCAATTGATCTATTCCCCCTCTTTGTTTCTTGGGGCATTTTTCCTTTTTTTTTTTTTTTTGTTTTTTTTTTACCCCTCCTTAGCTTTATGCGCTCAGAAACCAAATTAAACCCCCCCCCCATGTAACAGGGGGGCAGTGACAAAAGCAAGAACGCACGAAGCCAGCCTGGAGACCACCACGTCCTGCCCCCCGCCATTTATCGCCCTGATTGGATTTTGTTTTTCATCTGTCCCTGTTGCTTGGGTTGAGTTGAGGGTGGAGCCTCCTGGGGGGCACTGGCCACTGAGCCCCCTTGGAGAAGTCAGAGGGGAGTGGAGAAGGCCACTGTCCGGCCTGGCTTCTGGGGACAGTGGCTGGTCCCCAGAAGTCCTGAGGGCGGAGGGGGGGGTTGGGCAGGGTCTCCTCAGGTGTCAGGAGGGTGCTCGGAGGCCACAGGAGGGGGCTCCTGGCTGGCCTGAGGCTGGCCGGAGGGGAAGGGGCTAGCAGGTGTGTAAACAGAGGGTTCCATCAGGCTGGGGCAGGGTGGCCGCCTTCCGCACACTTGAGGAACCCTCCCCTCTCCCTCGGTGACATCTTGCCCGCCCCTCAGCACCCTGCCTTGTCTCCAGGAGGTCCGAAGCTCTGTGGGACCTCTTGGGGGCAAGGTGGGGTGAGGCCGGGGAGTAGGGAGGTCAGGCGGGTCTGAGCCCACAGAGCAGGAGAGCTGCCAGGTCTGCCCATCGACCAGGTTGCTTGGGCCCCGGAGCCCACGGGTCTGGTGATGCCATAGCAGCCACCACCGCGGCGCCTAGGGCTGCGGCAGGGACTCGGCCTCTGGGAGGTTTACCTCGCCCCCACTTGTGCCCCCAGCTCAGCCCCCCTGCACGCAGCCCGACTAGCAGTCTAGAGGCCTGAGGCTTCTGGGTCCTGGTGACGGGGCTGGCATGACCCCGGGGGTCGTCCATGCCAGTCCGCCTCAGTCGCAGAGGGTCCCTCGGCAAGCGCCCTGTGAGTGGGCCATTCGGAACATTGGACAGAAGCCCAAAGAGCCAAATTGTCACAATTGTGGAACCCACATTGGCCTGAGATCCAAAACGCTTCGAGGCACCCCAAATTACCTGCCCATTCGTCAGGACACCCACCCACCCAGTGTTATATTCTGCCTCGCCGGAGTGGGTGTTCCCGGGGGCACTTGCCGACCAGCCCCTTGCGTCCCCAGGTTTGCAGCTCTCCCCTGGGCCACTAACCATCCTGGCCCGGGCTGCCTGTCTGACCTCCGTGCCTAGTCGTGGCTCTCCATCTTGTCTCCTCCCCGTGTCCCCAATGTCTTCAGTGGGGGGCCCCCTCTTGGGTCCCCTCCTCTGCCATCACCTGAAGACCCCCACGCCAAACACTGAATGTCACCTGTGCCTGCCGCCTCGGTCCACCTTGCGGCCCGTGTTTGACTCAACTCAACTCCTTTAACGCTAATATTTCCGGCAAAATCCCATGCTTGGGTTTTGTCTTTAACCTTGTAACGCTTGCAATCCCAATAAAGCATTAAAAGTCATGA lncrn_neat1 human long Homo sapiens nuclear paraspeckle assembly transcript 1 (NEAT1) long isoform lncrna long non-coding ribonucleic acid lncRNA long non coding RNA long non coding ribonucleic acid long non-coding RNA lncrna human Homo sapiens Human GGAGTTAGCGACAGGGAGGGATGCGCGCCTGGGTGTAGTTGTGGGGGAGGAAGTGGCTAGCTCAGGGCTTCAGGGGACAGACAGGGAGAGATGACTGAGTTAGATGAGACGAGGGGGCGGGCTGGGGGTGCGAGAAGGAAGCTTGGCAAGGAGACTAGGTCTAGGGGGACCACAGTGGGGCAGGCTGCATGGAAAATATCCGCAGGGTCCCCCAGGCAGAACAGCCACGCTCCAGGCCAGGCTGTCCCTACTGCCTGGTGGAGGGGGAACTTGACCTCTGGGAGGGCGCCGCTCTTGCATAGCTGAGCGAGCCCGGGTGCGCTGGTCTGTGTGGAAGGAGGAAGGCAGGGAGAGGTAGAAGGGGTGGAGGAGTCAGGAGGAATAGGCCGCAGCAGCCCTGGAAATGATCAGGAAGGCAGGCAGTGGGTGCAGGGCTGCAGGAGGGCCGGGAGGGCTAATCTTCAACTTGTCCATGCCAGCAGCCCCTTTTTTTCCAGACCAAGGGCTGTGAACCCGCCTGGGGATGAGGCCTGGTCTTGTGGAACTGAACTTAGCTCGACGGGGCTGACCGCTCTGGCCCAGGGTGGTATGTAATTTTCGCTCGGCCTGGGACGGGGCCCAGGCCGGGCCCAGCCTGGTGGAGCGTCCAGGTCTGGGTGCGAAGCCAGGCCCCTGGGCGGAGGTGAGGGGTGGTCTGAGGAGTGATGTGGAGTTAAGGCGCCATCCTCACCGGTGACTGGTGCGGCACCTAGCATGTTTGACAGGCGGGGACTGCGAGGCACGCTGCTCGGGTGTTGGGGACAACATTGACCAACGCTTTATTTTCCAGGTGGCAGTGCTCCTTTTGGACTTTTCTCTAGGTTTGGCGCTAAACTCTTCTTGTGAGCTCACTCCACCCCTTCTTCCTCCCTTTAACTTATCCATTCACTTAAAACATTACCTGGTCATCTGGTAAGCCCGGGACAGTAAGCCGAGTGGCTGTTGGAGTCGGTATTGTTGGTAATGGTGGAGGAAGAGAGGCCTTCCCGCTGAGGCTGGGGTGGGGCGGATCGGTGTTGCTTGCCTGCAGAGAGGGTGGGGAGTGAATGTGCACCCTTGGGTGGGCCTGCAGCCATCCAGCTGAAAGTTACAAAAATGCTTCATGGACCGTGGTTTGTTACTATAGTGTTCCTCATGGCGAGCAGATGGAACCGGGAGACATGGAGTCCCTGGCCAGTGTGAGTCCTAGCATTGCAGGAGGGGAGACCCTGGAGGAGAGAGCCCGCCTCAATTGATGCCTGCAGATTGAATTTCCAGAGGCTTAGGAGGAGGAAGTTCTCCAATGTTCTGTTTCCAGGCCTTGCTCAGGAAGCCCTGTATTCAGGAGGCTACCATTTAAAGTTTGCAGATGAGCTTATGGGGGGCAATCTTAAAAAGTCCACAGCAGATGCATCCGGCTCGAGGGGCCATCAGCTTTGAATAAATGCTTGTTCCAGAGCCCATGAATGCCAGCAGGCACCCCTCCTTTCCTGGGGTAAAGGTTTTCAGATGCTGCATCTTCTAAATTGAGCCTCCGGTCATACTAGTTTTGTGCTTGGAACCTTGCTTCAAGAAGATCCCTAAGCTGTAGAACATTTTAACGTTGATGCCACAACGCAGATTGATGCCTTGTAGATGGAGCTTGCAGATGGAGCCCCGTGACCTCTCACCTACCCACCTGTTTGCCTGCCTTCTTGTGCGTTTCTCGGAGAAGTTCTTAGCCTGATGAAATAACTTGGGGCGTTGAAGAGCTGTTTAATTTTAAATGCCTTAGACTGGGGATATATTAGAGGAAGCAGATTGTCAAATTAAGGGTGTCATTGTGTTGTGCTAAACGCTGGGAGGGTACAAGTTGGTCATTCCTAAATCTGTGTGTGAGAAATGGCAGGTCTAGTTTGGGCATTGTGATTGCATTGCAGATTACTAGGAGAAGGGAATGGTGGGTACACCGGTAGTGCTCTTTTGTTCTTGCTTCGTTTTTTTAAACTTGAACTTTACTTCGTTAGATTTCATAATACTTTCTTGGCATTCTAGTAAGAGGACCCTGAGGTGGGAGTTGTGGGGGACGGGGAGAAGGGGACAGCTTGGCACCGGTCCCGTGGGCGTTGCAGTGTGGGGGATGGGGGTATGCAGCTTGGCACTGGTACTGGGAGGGATGAGGGTGAAGAAGGGGAGAGGGTTGGTTAGAGATACAGTGTGGGTGGTGGGGGTGGTAGGAAATGCAGGTTGAAGGGAATTCTCTGGGGCTTTGGGGAATTTAGTGCGTGGGTGAGCCAAGAAAATACTAATTAATAATAGTAAGTTGTTAGTGTTGGTTAAGTTGTTGCTTGGAAGTGAGAAGTTGCTTAGAAACTTTCCAAAGTGCTTAGAACTTTAAGTGCAAACAGACAAACTAACAAACAAAAATTGTTTTGCTTTGCTACAAGGTGGGGAAGACTGAAGAAGTGTTAACTGAAAACAGGTGACACAGAGTCACCAGTTTTCCGAGAACCAAAGGGAGGGGTGTGTGATGCCATCTCACAGGCAGGGGAAATGTCTTTACCAGCTTCCTCCTGGTGGCCAAGACAGCCTGTTTCAGAGGGTTGTTTTGTTTGGGGTGTGGGTGTTATCAAGTGAATTAGTCACTTGAAAGATGGGCGTCAGACTTGCATACGCAGCAGATCAGCATCCTTCGCTGCCCCTTAGCAACTTAGGTGGTTGATTTGAAACTGTGAAGGTGTGATTTTTTCAGGAGCTGGAAGTCTTAGAAAAGCCTTGTAAATGCCTATATTGTGGGCTTTTAACGTATTTAAGGGACCACTTAAGACGAGATTAGATGGGCTCTTCTGGATTTGTTCCTCATTTGTCACAGGTGTCTTGTGATTGAAAATCATGAGCGAAGTGAAATTGCATTGAATTTCAAGGGAATTTAGTATGTAAATCGTGCCTTAGAAACACATCTGTTGTCTTTTCTGTGTTTGGTCGATATTAATAATGGCAAAATTTTTGCCTATCTAGTATCTTCAAATTGTAGTCTTTGTAACAACCAAATAACCTTTTGTGGTCACTGTAAAATTAATATTTGGTAGACAGAATCCATGTACCTTTGCTAAGGTTAGAATGAATAATTTATTGTATTTTTAATTTGAATGTTTGTGCTTTTTAAATGAGCCAAGACTAGAGGGGAAACTATCACCTAAAATCAGTTTGGAAAACAAGACCTAAAAAGGGAAGGGGATGGGGATTGTGGGGAGAGAGTGGGCGAGGTGCCTTTACTACATGTGTGATCTGAAAACCCTGCTTGGTTCTGAGCTGCGTCTATTGAATTGGTAAAGTAATACCAATGGCTTTTTATCATTTCCTTCTTCCCTTTAAGTTTCACTTGAAATTTTAAAAATCATGGTTATTTTTATCGTTGGGATCTTTCTGTCTTCTGGGTTCCATTTTTTAAATGTTTAAAAATATGTTGACATGGTAGTTCAGTTCTTAACCAATGACTTGGGGATGATGCAAACAATTACTGTCGTTGGGATTTAGAGTGTATTAGTCACGCATGTATGGGGAAGTAGTCTCGGGTATGCTGTTGTGAAATTGAAACTGTAAAAGTAGATGGTTGAAAGTACTGGTATGTTGCTCTGTATGGTAAGAACTAATTCTGTTACGTCATGTACATAATTACTAATCACTTTTCTTCCCCTTTACAGCACAAATAAAGTTTGAGTTCTAAACTCATTAGAATTGTTGTATTGCTATGTTACATTTCTCGACCCCTATCACATTGCCTTCATAACGACTTTGGATGTATCTTCATATTGTAGATTTAGGTCTAGATTTGCTAGCTCCAAGTAATTAAGGCCATGTAGGAGAGCATGGTAACCACAGATAGAACTGGTATTATCCCAAGTGGTCTGCAGACTGCTGAGTGGGGATGGGATCTGCTCTCTGTTGAGAGTTGGTAATCATTGGTTTGAAATGTGATGAAACCACTCAAGCCAATGAAGGTGGGTGTGTAGGTGGGGAGTACTTTGCCATAATATTTTAAAACATTACCTGGTTAGAGTTCTAAGTGGTACTTATTTTTGTTTGGTTAGGGGAAAGCCTGAATAAAAACAGAAATGGACACATAATATGCATATTCCATAGTCTTTGGGAGGCTGGAATGTGCCTGGGATTTGGGTCTAAGTGTATGCGTAATTCTTACCTCACTAAAGAATTTGCCTTGTTTTTTTCCTTTTGGTGAGTGACTAAAACGTCTGGGCTTCCCTGTGTGCGTGCTACAGTAAGCAAGCAGAGGCTGTGCAAAGGTGTGAGCAGGATCACGTGGAATCTGGAGGATACATCTTGGCTTGCAAACTGCCTCTGTCTCCTGGGTGGGACTGTTCTGTCCTTGCACTGCTGTTCTGTGTTACCTCTTGGGGTGTAAGGTTTTGCTTACAGGAGACAAACTTTGGGCGTAGAATGGAAGCCACTGCCAGCCTCTGTGCTGAGAAGGAAGGTGCTTGTTTCAAAGGGAGCAGCAAGGGAGGCTTGTTCTACTCACCTGGGCCTGTTTGCCTGAGAAGGGGAGATAAGGGCTGAACTGGGACTAGCCAGGGGGACCAACACAAATGGTGGGGGATCATGACCTGAAGGATTCTTTCCTTCCCATGAGCTGCAGGGCTGGTTGCCGTCCTTGCAACTGTGTCTTATTTGCCTGTGCCGTTATATCTTGGTGACCCCTCCACGTGTACACTACTGACAAACGGGTGGAGTGCTGGGGAGAAGTCACTGTGCCGCCCACCTAGTAAACCTTCTGTCTGTGCTCATGGCATCTCCAAGATGGGGCACTGCTGTGTGCAGAATCCAGGGTCCTCTTTCTGCTTGCAACTCCTTTCCCTGGATGCCCCAGAAACAATCCAGGCCTCCTTTCCTATCTTACCCCTTTGCTTTGCTTTTTACCCCAGCACCTCTATAACCGCCTTCTCTTCTTTTCAGAACTCCTTGTTTCTCGTCCTGTTTTTTATGATTACAAAACTCTTGCTTCCACCCTGGAAGATAACTGCTATAGATGCCTGTATGTAAATGGTGCTGTCTCCAGCAACTGGCATGCTGAAGAAGAATTGATTCACGGGGTATAAATGTTGGGGATTGGAAGTGGGGATGAAATGGCACTTGTTGATACAGGAGCAGAGAGGTGAGGCCGACTGCTGAAGACAGCTCGCCACCCTCCTTGCCTCCACTCCAATCCAGGGGCTGGGGCCACATTCTTTGCCTTCATTTATCCTCAGATCAGGTGAGATCGACAGGAGGTGTTGATGGCAGTGCCAGCAATTATTGCTAATCCGTTTGCATCCTTATGCATAGATCTGAATTCAGACTTTGTGAATTTCCAGAGGTGTGGGTAATATAATAGAATTCAGTGAGTGGGCATGGCTGATCTTGTGCAAATTAAAAGTTATGGGGCATAAGAATAGCAAAAGTTGAACTTCTTTTAAAAAGGAAAGTACCCTGAGAGCCAGTATTGGTTGAGGCTCTTCAGTATGCCCAGGTTGGCAGCACTGAGAACCGCAGGAACGGCCTGTTGTTACAAAAAGGAGATTGACTCAGCTGCCCTTGGTGCATCTGACTGACTATGACTGCTGAGAGATTCCAAGGACCCTTAATGCCAGGGCTAACCTCTCCATGTGCAGTGAGACCTCTGGAGGAAGTGTCATCCTCTGGCTTTGTGTGGTACTCATTATGGTGCAGTGCGGGCATGAAATGAAGACACCCAAATAGGCTTACAGATACGATATGTTTTAAATGTTCGTATTTAACAAAAACATACTGACACTGTTTGGAAATGGCAACAGGAAGATAGCAAAATGAATACTAACATTACGAAAAGATGAACAGGTACATGTTCCAAGGCAGGTGGCTGTGAACTTCCTCTGAGTGAAGGCATCCCCTCCAGCACCTTTCAGCCTGCTAGTTAGGACGACCCGCCGCCACCCTCCAGGACCTCCAGCCCTGCACTGCCTTTCCTCTCTTTTAAATAATTCTTCATTGAGTTCTAATATGTAAAAAAAAAAAGTTTACTGTAAAGTTTGCAAATAAGGAAATTTTTTTTAAAAGTCCTCAGTAATCTTACCAGTAACAATTGTTATGGGCACATTTGCTTTTGGAAGATTTCTTTTGTATGCATGGGATAAGTACATTTTTAAACAAAAATGGGATTATGCCATAAATTCTATTTTGTGACTTTAATATATAGTGAACACCTTTTTTAATGATGACAGGATGTTCCCTTGCATGGCTGTATCAATTTAAACAATCTTGTTTCAATGGGCATACAGGGTATTTTCTAGTTTTTTTTTCCTCTTAGAAAATAATACTTGCGATGACTTTCCTTGTAGCTCAGACTTTTTCACGTCTGTTGTTATCTCTTTGGGAATGCTGAATACATACATTTCGAGAAGGAAATGACTGTTAAACTCTTAAGACTTCAGGTTCATATTGCTAAACTGCCCAGCAGGGAGGGATTTTTTCAATTAGTGTTCTCACTGGTGAGGCAAACCTGATGCCTTCCCCTCTTCCTCAGAACCGGCTTTATCACATTGAAAACCTTTGCTCCTCCGACGGATCGAGTCTGCTTTCCCTCTGGATGTGAGCATTGCTTTGTCTGCTGGTGACTGAACATCTCTACCTTGTGTCAATTGGCCATTTGTGGTGTGTGTGTGTGTGCGTGTGTGTGTGTGTGTGTGTGTATGATTTTCTAATTCCTAGTCATTTTTCTATTGATTGTTTTGCAAAAGCCATTTACATCTTAAGGATATTGATAATCTTTTGTTATATTTGATGCAAATATTTTTTTCCAGTTTATAGGTTGCCTTTTAATTTTGTGTTTCAGGTAGATAAAAGTTAAACGATTTTCTTAGGTTAGTTTATCACTGTGGTTTCTGAACTTGTTATGTGTAGATCTTTTCCACCCCAAGAGTACATAAATATTAATCCATACTTTCTTATGGAACTTGTATGGTTTCGTTTTTTACATTTAAACCTTCTTCCCCGTGGTGTGTGTTGTGGAATCTGTGTTTGTGTGAGGAGGGGCATGGTGCTCTCAGAACCCACCTCCTGTGGCCAGAGAGCCCTGTCCTGTGAGGGTGGTTGTCACAGTGGCAGGGTTCAATTCAGAAGACCTTGAGGGCAGGCTGATGTTTCCTGAATGGGCCCCTGGTTGTTGCTTGTCCCTGACTCTCCATTTCCCCATCTGAGTGGATTTGGACCTAATAGGGCACTGGAGCTGGTTCGAATCCTGACTGGACTACTTGGCAACTTTATGTCTGGGAGCAAGTTACTTAACCTCCCCAAGCCTGTGTCTGTGAAATGCGGGTAAATGAATGTAGATGTTTGGCAGCAGCTACTCCTTGTTGAGCTCTCACAGTGAACTCTCCTGCCTCTGCCCTCCTTCCCCGCCTCCCCTGGTGCCTAGCGTCAGGTCTAGCCACTTCCTCCTGGGCCCCTCTCCCTTTTCTGTGGCTGGCTGCCTGCCCGCCTGGCGCTGGACCTTTCATGTAACGGGAATCAGCATGTATATTCTGGTCTGGTCTGTTTCTACACTTAATTTTGTTTCCAGTAGTATTTCCCTGTACCGGCAGAGTTCACAAACACATTTGAAGAGGCTTTTTCTCAGGATTCTTAACCTTCCCAAAGGAAGTCCCATGGATGGGTTTCTAGAAGTCTATAAATGCTCTGAAATTGTATTTTTCTGTGGAAAGCATAACTTTCATCTGCTTGTTCGTGCTCAAAAAAGATCATGAATGAATGATTGCATGATTTTATGCCATTGTGCTTATACTAAAGGATATGTAGCCCATCTCTTGAGCTGTTAAACTGTTTTGACTACTTTAAATCGTGCAGCTGTGAGCATCTCTGTAAATTTAGTGTACACATGTATCCCCTGGAGTGGCATTGCCTCGGCAGTGAGCACTTATGGTTTTATAACTCTCTTCACAGACTCAAATGACTCCAGAAAGCTACACTTCCTGTTGTGAGTATATGATATCCATTTCCCTACATAGCCACTAACATCAGGTTTTTACAATTTTATTTATTTCTTGCTACTTTAAGAAATTTTTGTGGTGAAATACATATAATAGAAGTTGACTATCTGAATCATTTTTAAGTATACATTCAGTAGTGTTAAGTATGTCGCCATTGTTGTACAACCAATCTCCAGAACTTTTTCATCTTGCAAAACAAACTCTGTACCCATTAAATAACATTAAACATTCCATTCCCTCCAGCCTCAGCAACCCCATTCTACTTTCTGTTTCTGTGAGTTTGACTATTCCAAGCACTTCATATCAGTTAAATCATGAAGTATTTGTCTGTCTGTGACTGGCTTATTTCTCTGAGCACAGTGTCCTCGAGATGCGTCTATGTTGTAGCATATGTCAGAATTTCCTTCCTTTTTAAAAGATCCAAATAATATTCTTATTTTATATCTTTTTTTTATCCATTCATCCATTAGTGGACACTTGGGTTGCTTTTGGCTATTGTAAATAATGGTGCTATGTACAAATATCTATATTATTGTATTTACAAGTATAATGCTGTAATGTACACACATCTTTTTGAGATCCTACCTTCAGTTCTTTTGAGTATATAGCCAGAAGTGGTATTACTAAATCTTACGATATTTCTATTTTTAATTTATTGAGGAACCACTGTAGTTTTTCATAGCAACTGCACCATTTTACGTTCTCACCAAGAGTGCACAAGGGTTCCGAGGTTCCCACATCCTCCCCAACACTTGTTATTTTCTGCTTTTTTTAGATTGCAGCCATCATAGTGGGTGTGAGGTGACATTTCATTGTGGTTTTGATTTGCATTTCCCTAATGAGGAGTGATGCTGAGCATCTTTTCATATGCTTACTGGTCATTTGTATGTTGTCTTTGGAAAAATGTCTATTCAAGTCCTTTGACTATTTTAAAAATTGGGTTATTAGAGTTATCGTTGTTGTTGACTTGTAGGAGTTTCTTTCTATATTCTGGATATTAATCCCCTATCAGATATATGATTTGCAAATATCTTCTCTTATTCCATAAGGTTACTTTTTCACTTTGTTGATTGTGTTCTTTGATGTATAGAAGTTTTTAGTTTTGAAATAGTCTAATTTATCTGTTTTTACTTTTGTGGTCTGTGCTTTTGGTGTCATATCCAAGAAATCCTTGCCAAATCCAACGTTATAAGGTACTTTTAAGGTATTTTAGTTGTCTTAGTCTATATTTCTGTACTCACCTTTCTTTATCCACTCATCAGTTGATGGGCATGTAGGTTGGTTCCATATCTTTGCAATTCTGAATTGTGCTATGATCAGGTGTCTTTTTAGTATAATGATTTACTCTCCTTTGGGTAGATACCCAGTAGTGGGATTGCTGGATCGAATGGTTTTTATAATTTTCTATTTTACCACAGTTTCTCTCTGCATTTTTCCTCTTTGACCACTAACCATGTGAAATTCTCATATTGACCTTTATAATGATCATGAACTCTTAGTATCATTGGGAAGGCCACATTTGCCACTTATGATTGTAAACCTTATCCTCCATTTTTCCTGTTATTGTTGGTGCAAAAAGCACCTATTATACCAGGACTTTAAAAATCAGTCTGATAAGTCTTTGATAAGTCTAATAATAATAACTGATAAGTCCATTGAATTTGCTTCTGATTACTTTTTCTTTAGTAGCTAAACATGTATGTACTCCTATGATTACAATGAACACTCCTCTCCATTTAAATTAATTATTTACATTGATGAAATAGCAAAATGTTAATGACTAAATACTGTCTTGGTTTTTTCGTTCCAGGTCAGTCAATATTAACTTCTTATAATTTTCTTTTTTTTCTTTATGTGTGTGTGTGTGTGTATTTTTTTTTTTTTAATTTCAATGGCTTTTGGGGTACAAATGGCTTTTGGTCATATAGATGAATTCTACAGTAGTGAAGTCTGAGATTTTACTGCACCGGTCACCTGAGTAGTGTACATTGTACCCAATATGTGGTTTTTTATACCTTGCCCCCCTCTTACCCTCCCCACTTTGAGTCTCTAGTGTCCATTATGTCACTCTGTATACCTTTTTGTACCCATAAGTTAGCTCTCACTTATAAGTGAGAACACACAGTATTTGGTTTTCCATTCCTGAGTTGCTTCACTTAGAATAATATCCTCCAGCTCCATCCAAAATTGCTGCAAAAAAAAAAAAAACCACAAACATTATTTTGTTCTTTTTTATTGCTAAGTCATATTCCATGGTGTAGAGATACCACATTTTATTTATCCACTCACTGGTTGATGGGTTGGTTCCACATCTTTGCAATTGTGACTTGTACTGCCATCAAGTGTCTTTCTGGTATAATGACTTCTTTTCCTTTGGGTAGATACCCAGGAGTGGGATTGCTAGATCAAATGGTTCTTAACATTTTCTCTCTGGATCTATTTCTGGAAATTTTAGGCTCCAGTTTTTGTTGTTGTTGTTAATAAAATGCAATGGAATGTAATGATCATCACTTTTCATTATGCTTTAAAATCTGGTAAATGGAGGCTAGAACACTCCTGTAAGGCAAGAATATTCTCTCTGTTGGAACTCAAATACACAGAACTGGGTAAATCTCAATCTTAATCTTTGATTCAGGACACAACATGGCTCTCTTTTACTTGCTTTCTTTAATTGTTTTTTAATAATGTGGTAAGCATTTCTGAATCTCCTATCCAATACAAAAACTAGGACAATACAGACAGTAACTCCTATGGTTACAATGAACACTCCTCTCCACTTAAATTAATTATTTACACTGATGAAATTGAAATAGCAAAATTTTAATGACTAAATACTGTCTTTGATTTTTTGTTCCAGGTCTGTCAATATTAACTTCTTATAATTTTCTTTTTTTTTCTTTATGTGTGTGTGTGTGTGTGTATATATATATATTTAATTTCAATGGCTTTTGGGGTACAAATGGCTTTTGGTCATATATATGAGTTCTACAGTAGTGAAGTCTGAGATTTTACTACACCTTCCACTTATGTGGTCCCACACCACCCGCCTCCCCTGCCGCCTCCTGCCACCCCCTAGGCCAAGGTAATAATCATCCTGAATCCTGGGTTTATCTCTCACTTGCTTTCTTTTCATATAATTTTGCAAAAGAATCTGATCTAAATGTGTTTTTCAGAGTATATATTTATATTTTAGCTGTTCTTAGAGAAAATTTATTATTTTGCATGTAATCTTATGGAACATTCTCATTTAATACCATGGTAAGATTCAGCCCTTGCCCAGGGGATAGTTCATTTAGTTTGTTTACTGGATAGAGCTCATCATGTGACTATACCTCAGTTAGTTTATCAGTTCTCCCATCCATGGTGACTAGGTTGCCTCTCAGCCTCTCAACAACACTGTTTCTCAGTGTCCTTGTAGAAGTGATATGTGGGTGTTTTCTCCTTACACAGAGTTGAAAGGTGACGACAACAACGTTGGCACTACCAATCCCCCACCCTCCAGAGGGGTAACCAGTGTTACCAGTTTGCTGTGTTTCCTGCTACACCTCGCCTTATTCACTTCCATTTGTATCTGAAAAACGTGTTGCATGGTTTCTTTTCTATAGAAGTGGTAAAATGCTATTGTGTCCTGTACATTATTGATTACTTTTTTTCATTTAACAGTAGGGAGATGCCTGGGAGTACACAGAGAACTGCCCTCATTGTTTTCAACTTCTGCACTGTATGTCTGTGAGTTTAGCCATTCTGCTGTTAATGGAAATTTACAGTATTCTAATCTTTTGATATTACAAACAGTTCTGTGCGATCATCGTCATACACAACCCCTTGTGCACAATGCATGAGTGTTTCTCAGGGTAGGTACCAAGAAGTGAAATTCCTGGGTCATAGGGCGTGAGTCCGACATTTTTCTCCATTCTGCCCTGTTGCCCTCCAGAGTGGGTGTCCAGCTTTGCATACCTAAGTATGAGAGTATCTGTTGTTCATATCCTCTACGACGCTCCATATATGAAACTTAAGTTTCTGCTAGTTGCCATCTTTGATCTATCATGTATGCAGTGACCTACTAAGACTGTAATTGGTACAGTAGATTCTTGTCATCTGTGTGTGAATTTAGCATTCATGGGCTTAATGCTGACAAGGCCCCCAGGGTCCAAGACATATAATCATGTATAATTTTGTCAAGGTATAATTTTTTAAATTGCTTTTGTCATGTGTCTGCTGGTGATGCCCAACCCAGTGCTCTGCACCCAGGTCACACTGTGGCTTTGTCCTCTGCTTATGCCTGCATTGCAGCAACTGTCCTGAAGAGACCAAAATTATGCAGATTTAGGTAAGTCCATGGCTAATGTTATTATATTATGTGCTATTGTAATGGATGGGGCTGTGGAGTGTATGAATTTATAAATCACTGGTCTTGTAATTAAAATTCAAACACTATAGAAAAAGGCCATGTAGAAGATAAAAGTTCCTCTATAATCCCGGACCCCTAAGATAACTACTAATGACAACTTCATTTATATTCCTTCAGACATTTTCTGGCTGTGGATGTACTAAAATGTATCCTATTATTCTCTGCCCTAAAATGGAATCATACAAGGTGTACTGTTATTTTTATGGCTCTATAACATGTCATATTGTACGTGTTGGTATGGTCATTTTAACCATTTTTCTAGTGATGGCTTTGAGGTTATTTGCAGTTTCCTAGCCATCTCAAAGTGTGCTGCGGGGATCTCTTTTGCATCCCTCTGGGTGCAGAGCTGAGGCACCCAGAGGCAGTGTCCAGAGGAGGCAGCATCTGTAGGTGTCTTCACCTGCTCTGGCTCTTGGCACATCTGGTTGGTGACACTGTTTTGTGAGATGGGTTGAAAGCACGTGCTGCCAAAATAGAATAATGTTGGTCCTCTCCTCATGTGCCGTGGAACTGGGGTAAAACTGCGTAGTGGCTGCAGCTGCCTGTCCATACCGGAATCGAGTATAACACGGTGCCTGGCTTAGCACAAAACAGTAGTGGGTCCTGCAGGCCCCAGAGTCTAATTCCTGGTATTCTTTCCCCTACACAGATTAAATAAACCAAAAACAAACTATTCTAGGAAAGCGTCTGTGACATTTGTAAAAAGTGGTATTTAATGATCTTTTATTCACTTGTCTGTTTAGTTTGTTGAAATCTTAAGTGGCATCCTGGTCTGGGAAGGAGTGCTGTCTGCGCCTGCCCTCCGCTGGGCACAGCGTGGCTGCTTCAGGGGCTAAGCACACACTTTCTGTCTTCTAAAGGGCCGCCACATGCCAGGAGCTCAGGTGTGAGCCCGGCTCTGGCTCTTACCTCATAGGGTCACTCATAGGGGCACAGGGAGCAGAACATTGTACACAGCGAGGCACCACCCGGCTTGGCATCTGCCTCGGTGGACTTACTACCTCTAGAAGGAAATACCTGAGTTCCTCTGGCCTCAGCTCCTAGAGTGACTGGTGTGCTGTCCCTGTTACTCTTCTGTCAAGGTGACAACTGTGTGACCCATCATCTGTGTGTCAAAGCAAGGCCCTGCCTGGGCCTCTGCTCCTGTGCTGACCCCAAAGGCAAATGCTTTGCTAGTTTCCTTCCAGTTAATTTCACCTATGAATAGATGTGTGAAAACTGTTCAAAGCCATACCTGCACATGTTTGAACTTCAAACCCTGTGGGTGATTCAGTGGCATCTTTCTCTAACCCCCAGCCTCCCTTCCCACAGAGGCCACCGTCATGGCCAGTTGCTGCAGTTTCTTTCCAGAGAACCTGTGTATGTGTAAAGCTGTACAGGCGTGGGTACACCACACAGCCTGTCTTGCACTGTGGACTGTTGAGTTACTAGTACATCTAGGTAAGCACCGCATATCTGTATTCATGTCTGCCTTGGTCTTTTCAACATCTGTGTGGTAGCCGTGTTTGAATTACCCATTCCCTTTTTGGGGAACCATTAAGTTGTTTCAGCAATTTTTACTGTAGATAAGGCTATACCGCATATCTGTGTACATGGGTTTTTATGTACATGGGCAAGTATATCTGTGAGAGAAAAGTTTCCTCAGGAGGAATTCTGGGCACAGCATGTGTAAATTTCTAAATATGATGGACACCCCCAGCTTCCACCTCAAGGAGGTTGGTCCCATTGACATTTCCCCACACCTTCACCCAGGCTGTGCCCTTAAACTTGGTTATTTGTCAATGTGAGAAGTGGAAAATAGTATTTAATTGTAGTTTGGATTTGTATTTCTATTGGGTTGTATACTTACTGATTAATAATAAGAGCTCTTTACATATTAAGGAAATTAACCCTTTTCAAATACATTCCTATTTCTCACTAATCTTTAAGTTTTATTGTAATATTTTGCTCTTTAGTTTATATATATATGTATATATATATATATGTATATATATATATATACATATATATATACATATATATATACTAATTTTCTTTTATGGTTCCTGGATTTTGTGAGTAGTTTGAAAAGGCTAATCCAGCTGAAGATTTTGTTGTTGTTGTTAAACCCCATGTTTTCTCCTAACTCTTTTTATTTTTATTTTGGAGGACTCTATCTAGACTTAATTTTAGCATAACAAGTGACAGGGTTAGTTAGCCTGTTGTCCTTACACCATTTTCTGGCTAATACAGCTATTAACTATTGATCTGTCTATTCACGTGCCAGTTCCTAATGGTTTTACATAGTGTAATCTGCACTTCAAAATAGCGAAGGGAAGCCCTACCTCATTATTCTACTTTTCCAGAATTCTCCTGGCTATTCCAGGCTGCATGTTTACCTTAACCTTCCCTGTGATGTCTTCATGCCGTTGTCTTCTTATGCAAGAATAAGGTACGTCTTTCCATCCACTCACGTCTATTTAATTTGACTTTGCATTACACAGAAAGCTGGTCTTGGTCTGTCTACCTCGGCATCTAGTTGTCCTCACTGCCCCCTAGCCGACCCCACCCCATCTGACTGACTACCCCATCACAGAGTACTTTTATTTACGTTTTGCTCTGCCTAATGGTTACTTGATACTGTCACGCCGACAGTGTCCAGTTCAGTGGTCTTTGCAGTTGAAATGCTCCCGTACACACTGTCTTGTTAAAAATGCCAGTAAGTTCATACAAACCCAGCTTGCACCCAAGGTCACATTCAGAGAGCGTAGGGCTGGGATGGGTTGTTTTCCAAGCTTCTGCCACTGTGTGGCTAGCTCTTCCCACTGGGAAGTTCTGTGTACCCGGAATGTCGGAGTGGAGTCCTGTTCTAGTGTCCAGCACCTGACCCTGTGCCCAACCCCTCAACAGCCTATTCCTGCTGTCCACAGCCTGCTGGAACTTTTTACAAAATATGTTGCCATGCTGGACCCTGGGCACTGGACATAAGCCCCCTGGCAGCCTTTTTCATGTCACCCAAAGGGGTAATTGTCCTACTGGTGGTCTGTAAGATGAGTTAGGGTGACTTGCTAATAGACATTGTAAATCTTAATATTTATGTATGTATTTTATTATTACCGGTTTTCCATTTATGATGGTAATATTGTTTCTTCTAAGAATATTTATTTTTCCTTCTAAATATTGAGATAAAATTCATGCTTTTGAAATGTTCTATTCAGTGGCTTTTAGTATATTTGCTATGTTGTGCAACCATCGACACTATCCATTTCTAGAACTTTTTCGTCATCCCAAACAGACGCTCTGTATTCATAAAAAAATAACTTCCTACCTGTCTCTCCCCCTAGTCTTTGGTAACCTTTGTTATACTGGTAAACTTTGTTGTGCTCTCTGTCTGTGTGAATTTGCCTATTCTAGGGGCCTCATATAAGTGTAATCATACAGTATTTGTCTTTTTGGGTCTGTCTGATTTCACTTAGCGGGTTTTCAGGGTTCATTCATGTTGCAGCATATAACAGTACTGCGTTCCTTTTTCTGGCTGAATAATATTCCACTGTATGGATAGACCCCATTTTGTTTATTCACACATCATTTGGACATTTGGATTATTTCTGGTTTTTGGCTATTATGAACAATGGTGCTATGAACAGTTGCGTACAAGTTTTTGTGTGAACATATGTTTTCAATTCTCTCATTATATACCTAGGAGTAGAATTACTGGGTCATATGGTAACTGTATATTTTTGAGGAACTGCCAAACTATTTTCCCACGTCCATGCACCATTTCACATTCCCACCAGTAAGTAAGAGGGTTCCAATTTCTGCGCATTCTTGCCAACACTAGTTATTATCTGACTTTCTGGTTATAATCATTCTAATGAGTGTGAAGTAGCCTCTGGTGTCATTTGGATTTGCATTTCTCTGATGAGTGATGCTATCAAGCACCTTTGCTGGTGCTGTTGGCCATATGTGTATGTTCCCTGGAGAAGTGTCTGTGCTGAGCCTTGGCCCACTTTTTAATTAGGCGTTTGTCTTTTTATTACTGAGTTGTAAGAGTTCTTTATATATTCTGGATTCTAGACCCTTATCAGATACATGGTTTGCAAATATTTTCTCCCATTCTGTGGGTTGTGTTTTCACTTTATCGATAATGTCCTTAGACATATAATAAATTTGTATTTTAAAAGTGACTTGATTTGGCTGTGCAAGGTGGCTCACGCTTGTAATCCCAGCACTTTGGGAGACTGAGGTGGGTGGATCATATGAGGAGGCTAGGAGTTCGAGGTCAGCCTGGCCAGCATAGCGAAAACTTGTCTCTACTAAAAATACAAAAATTAGTCAGGCATGGTGGTGCACGTCTGTAATACCAGCTTCTCAGGAGGCTGAGGCACGAGGATCACTTGAACCCAGGAGGAGGAGGTTGCAGTGAGCTGAGATCATGCCAGGGCAACAGAATGAGACTTTGTTTAAAAAAAAAAAAAAGTGACTTGATTTAAGGGAAAAAATGACTGGCTATATTCAGTCAGATATGGCAAAAAGTCTCAAGGTGTTAATGTGAATGATTAAGGTCTTGGGGGGGGTGTCCCCTATCAGACTACAGGTGTTTAGAGGCACAGAAAAAGGTGCAGTTGGGTTCTTAATGTGAAATGATGAGAAGCACAACTCCAGTGTGTCTCTTTGTGTAGAATGTCAGCAGACACCCCCTGCTAGATGTGCTGGATCATGGGAAAGCATTTCCATTTGTTACTAGATTGTTCAGAAGTTTTAATTTATGATGGGTGTGGTGGCTCATGCCTGTAGTCCCAGCACTGTGGGAGGCTGAGGCAGGAGGATCATCTGAGGCCAAGAGTTCAAGATCAGCCTGGGCAACATAGTGATACCCTATCTCTTAAAAAAGAAGAAGTTTTTAAATTTGAAATAATAATAGGTACTGGATTTATGCAAATGTCTTTTCTGCGTCTTTTGAGATGAGTATCAGGTTTTTTTTTTTCCTTTTATCATCTGATGATGAACTTAATGTTTCCATTTGTATTAATGGAATACTAAGTCCCTCTGTGATTTCTGAACCAAGCTATTCCTAGGCCTGAGTTTTATTTTGTTGACACAGAAATAAATTAGAAGGCCAAGCGTGGTGGCATGTGCCTGTAGTCCTAGTTGCTGAGGTAAGAGGATTGCTTGAGCCCAGGAGTTCAAGGCTGCAGCAAGCTTTGATTGCGCCACTGCACTCCAGCCTTGGCGACAGACTAAGACGCTGTCTCAAAAAAAAACAAAAACGACAAAAAAAAAACAAAACAGAAAAAATAAACTAAGGCAATGACAGTCCCTGGCAAATGCTGGGAGGGAGGCAGCAGTGGTCAGGGAAGGTAACCCTGAAGCAGGACTTGTAAAGCAAATAAGATTGGGAGGCCAAGGTGGGTGGATCACGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATAGTGAAACCCCGTCTTTACTAAAAATACAAAAAAATTAGCCAGGTGTGGTGGTGGGTGCCTGTAGTCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCTCGAACCCAGGAGGCGGAGGTTACAGTCAGCTGAGACCGCACCATTGCACTCCAGCCTGGGTGACAGAGCAAGATTCCGTCTCAAAAAAAAAAAAAAAAAAAAAACCAAGAAGAAAAGGAATGAATTAGAACTTCTTCTGCTTGGACTTAAGGGCATCATCAGGCAGGTTTTGGGTAGGATAGCAGGGGAGGCAGAGACATAGTCGGGGTCAGTGGTCATGAGTGTGGCTTTGAGCCCAAAAACTTGGTTTCTGTTCCCTACTTTGCCACTCAGTAGTGCATGACTTTGGCCAAATTTCTTAAATTCATGAAGCAAGTTTCCGGGTGAATGAAATGGGGATAAAAATAGTGTTCAAACCTATCCGTTGGTTTGTGTGAAACTGAAATGAATAGTATCGTGCAGGTACTTGTGAGCAAGGGGAGCTGCTGTTTCCTGTCCCTTTATGATGGGAAATATCTAGACAAGTTCCCAACCCTCTGCACTGCAGGCTGCATGGCACGGAGGGTCTTGTAACACCAGCTGGGGCTGGCCTTCTTTTAGGAGCTTCAGTGGTTCTGAAAACTTTTATTTGTTTGTTTGTTTTAGTAGATGTGGGGTCTTTCTGTGTTGCCCGGACTGGTCTCAAACTTCTGGACTCAAGTGATCCTCCCCCGCTCAACCTCCCAAAGTGTTGGGATTACAGGTGTGAGCCACTGTGCCCAGCCTTGAAAACTTTTTCAGGTTCTTCCAGGGTTACTGGGCTATTAAATATTTCTATTTCATTATAAGTCAGTTTTTCAAAGTTATATTATCTTAATTACCTTTTTTATATGTATTAGTGTAGAGTAGCATTTTATATTTTGATATCCTCCTTATGCATAGTTTTTCACTTTTTATTCCTAGTTTTTCGTTTTTAATAAGACTTTCAAGAAATTTATTTTATTGGCCTTTTGAAAAAAGCAGCTTTAGATAAAGTAAGCAGTTCTGCTTTCATTTTATAATTTATTTCTACTTTTGTTTCATTAATCTTTTCCTCCGGCATGCCTTGGATTTTGTTGTGTTACTCTTTTTCTAGAGGCTCGCATTGTGTGTCTGGTTCACTTATGATCACGCTTGCCTACTTTTAAGAATGGAAGAGGGGAGGTGGAGGGTGGCTGCACAGTCGAGGGTGTGAGGCAGTCTTGCTCTAGCCCCACCATGCCCTCAGCCCGCTGTGGCCACGCTGGTTCCTCAATTGCTGGGGCGTGCAGTGTCTGTAAGGGAGGCTACTGATGCCATCCGAGGAAGATGTAAGGTTTCGTGTGGGCAGCGAGAGCCTAGCAGGCATGTGGGGTGCCCAGCAAAGGGTAACAGTGGACAGTTGTTGCCTCATTCCACAGAGTTTTGATTTTTTTTTTTTTTTTAATGGTCACTCCATCAACATCCCCCATGGCCAGAGCCTGAGCTGGTCCCCAGAGACACAGGCATTCAGCTGACAGCCTCGCCTTCACGCTGCTGCTGTTCTCATGGGGGACAGGCCTCAGGTGGCAATGCACAAATCATTAGTTAAGGGCAGTTGTGACAGTTACCAAGGAGTGTAGTCCCCCGCCCCCCGCCCAGTGAAAACAGCCCTAACCAGGGGTGGGGACCTTTGGGCTCTGACCCGAAGGGTAGGAGAAGCTGGAAGGACAGCATTCCTGTCTGCGAAGGCAGGAGCAAAGCTGCCAGGCTATGAAGGAAATGGCTGGAGCCTGAAGTCATGCAAGCTGGGGCTGGCAGGGACAGGGCCAACTTCCAGGCCTGGGGGCCACCATGAGGATTCAGGACGTGACCCCCAGGGCACATGAAGGCCTTCCATCTGTATTTAAGAAAAGACTTTATCAGACGAGTATGGTGGCTCACGCCTGAATCTTAGCACTTTGGGAGGCTGAGGCAGGTGGATCACGAGGTCAGGAGTTCAATACCAGCCTGGCCAATATGGTAAAACCCCATCTCTACTAAAACTACAAAAATTAGCCAGGCATGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGAAGAATCACTTGAACCCGGGAGGTGGAGGTTACAGTGAGCCAAGATCGCGCCACTACACTCCAGCCTGGGTGACAGAGTGAGACTCCGTCTCAAAAAAACCAAAAGACTTTATCTTATTTCCTATATGTTTGTGGTTTCAGTCCTGATGTATAATTTGACCCTAGTTAGAATGGTTATCTGAGGAAGTGGCCTGTACGATTTCTGCTTTTTTAAATGTGTGGCTCCCTTTCTTCATTGATTAACGTATGATTATTTTTATAAATGTTCCATGGCAGTGGGAAGGGATTCTCTGTCACATTCCACATCTGGATCAGTTCCTCCCCATTTTGTTGGTCAAATCCGATCTGCCATATCCTGTGTAATGACAAGTGAGTTGCATTCTCACCGTCACTCCTGGGGTCTCTCCGCTTCCCCTGAGCTGGCTCAGCAGTCTGCTCCATGTGTTTTGATGCAGGGTGACCCATTGGTATTCCCGACACTAACGCCCCCGTCTGTGGACTGCTTGCTGCTTGGGCTTCACTGTGTCTGGTGTTGACAGTGCAGACCTAAAGGTGTGCACACATGTGCACACACACTCCGCTGTCTTCTTGTTTGCACTGGACTTAAATATCTATGAGGGTTATTTTCAACTGCTGAATTTGGAATGATTTTTATATCTTTTCTGCTTTCTGCCCATGTACATGTGTTTATTTTACACTGTTGTGATTGGTAGTTACTATGTGGGGACACAATTACTTGGGCTGAAATAATCCACCTGTTGTGGTTGGGGTCCTCTGGGGCATTCCAGGGTGAGAGGTTGTCACTGCCACCTGGGCCATGTGGGCCGGCACCAGCATTTTGTGGTTACGAATTCTACAGTCACAAATATCTTTGGGCAAATCCCCTTCTATACCTCAAGGCAGCTTTTGGTTTGCAACCCCACTGGCCAGAGGGAAGGGCCAGTCACTTGGCTCTCTCACTGCCCTGCGCCCCAGATGGTTCTAGGGCTGCTGTTTTCCCTTGGCCCTGCCAACACCACTGTTTTTACTTCTGCTCATTGGCTGAGTGCAGTGGTTCCTGGAAGCCAGTGGCACGTTTCCCCGCGTAGCTCGCTTATCCCACAGCACACACCCAAGGGTTCTGTTGCTAACACGCTGAATTAATTCTTTGCTCATCTTACAGAGTGTGTTTTGACTGCCCCCATTTCTGAGGCCTTGTAAGGCCAGAGCTTTGTTGCTTCATCGGCAGGTTGGGACTTAGATGGCCGTGAATGTTTCCTCTCTGCTGCTGCAGTAAGTAAGTGCCCGCACCATAGTGTGTTTGGAGGCTGAAGTTGAAGCGAGGCTGTGAGGGGAGATGGACGTGTGAGGAGGGATGATGGGGCTTGAGCAAAGTGGGGGAGGGGGCAAAGGCAGTTGGCCCAACACATTCCCCACCCCTTTGAGAGGTCTGAGGCCTGCAGACCTGGCTCGGAGCCCACCTGGTAGTCCTCAGACTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAAAAGAGAGAAGTTGTGGAGAAATGGGGGGCTGATTCTGCTCAGATTCATCAGGATGAGTAGAAGGCACCCAGCTCTCACCCTGGCCTGACATGTGTGTCCCTGAGCAGGTTACAGTCCTCTCTGAGCCTCTGCTTCCCATCTGGACCCTGCTGGGCAGGGCTTCTGAGCTCCTTAGCACTAGCAGGAGGGGCTCCAGGGGCCCTCCCTCCATGGCAGCCAGGACAGGACTCTCAAATGAGGACAGCAGAGCTCGTGGGGGGCTCCCACGGACCCGCCGTGGGCCCAGGGGAGGCAGAGCCTGAGCCAACAGCAGTGGTGCTGTGGACCGTGGATCCTGAGGGTGGCCTGGGGCAAGTACCGGCTGAGGGTCCAGGTGGGCTTTGTGTACCTTTGGGTCCTGGGGCCCTGGTGACTTGGACTCCAGGTTAGAGTCAAGTGACAGGAGAAAGGCTGGTGGGGCCCTGTGCTTCCGACTTCATTTCGAGTGATGGCAGTTCCCAGGAAGGAATCCACAGCTGACGGTGGCTGACAGATCAGAGAATGGAAGGCGAGGCAGGCGGGCGTCTGCGTGACCTCAGGTGCTTGGGGCCCAGCAGACCCAGAGAACCATTTCCACTAGGCCAGGGTGCCGGAAGTGTCCACAGGTCTTAGATTCCCTGTTCAGATGAAAAGATTTGTGCCTTTAATGATAAAAGTGATCTGCATAGAGTCAAAAATTCAAGCCATGGGTATAAAATGCAAGTAAAATCCCTGCCCTCACCTATCCCACCCTACTACACAGAGATGTCCTCTCGAGTTTCCTAGACTCACTCTGGAAATTTCTGTATACACACAGAAGCTTGTGCCTCTGCTCGTGAAGGCAGAGGGAGGGAGAGCTGAAGGGCCAGCACCTTCTCACCTGTGGGCCCCCTCAGTGCTCGGTCCCAGAGCATGCAGGACTGTGCCTCGTGTTCAGTTTGCTGGTCTGACTTCATGCTCCTTGGGCAGGATATGCATGTGCCATGCTAGGAGACATGTGGATGTGAAGCTGGGGGACAATGTCCCCTGGCTATGCCTTTACAAGGGAAGTAAGGAAGGTAGGAGGTGAGCCTGGGAGGGAGGGAGGGAGGCGCGGAGCCGCCGCAGGTGTTTCTTTTACTGAGTGCAGCCCATGGCCGCACTCAGGTTTTGCTTTTCACCTTCCCATCTGTGAAAGAGTGAGCAGGAAAAAGCAAAA I have not added the sequence because it is more than 22700 nucleotides long. should I? hsa-mir-185-5p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA human Homo sapiens Human UGGAGAGAAAGGCAGUUCCUGA ago2_human Protein argonaute-2 AGO2 Argonaute RISC catalytic component 2 EIF2C2 Eukaryotic translation initiation factor 2C 2 PAZ Piwi domain protein Protein slicer protein protein human Homo sapiens Human MYSGAGPALAPPAPPPPIQGYAFKPPPRPDFGTSGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA DIP protein Q9UKV8 original sequence version: 1(def) DIP protein Q9UKV8 original sequence version: 44 DIP protein Q9UKV8 original sequence version: 60 DIP protein Q9UKV8 original sequence version: 62 DIP protein Q9UKV8 original sequence version: 89 DIP protein Q9UKV8 original sequence version: 85 DIP protein Q9UKV8 original sequence version: 87 DIP protein Q9UKV8 original sequence version: 99 DIP protein Q9UKV8 original sequence version: 117 DIP protein Q9UKV8-1 original sequence version: 117 DIP protein Q9UKV8 original sequence version: 145 DIP protein Q9UKV8 original sequence version: 108 5C8552C43FC81345 hsa_mir_185_5p-mrna_igf2_1-1 4 8 predetermined predetermined participant predetermined tag luciferase tag visualisation by luciferase assay tag luciferase regulator regulator modulator regulator neutral component neutral component engineered engineered purified purified 6 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered tag luciferase tag luciferase tag 3' range 3' range 3' range 3' range r.759_765delinsagagagg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain TCTCTCC AGAGAGG physical association physical association FIG 4 C D down-regulates quantity by destabilization hsa_mir_185_5p-mrna_igf2 long-1 1 8 predetermined predetermined participant predetermined tag luciferase tag visualisation by luciferase assay tag luciferase regulator regulator modulator regulator neutral component neutral component engineered engineered purified purified 6 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered tag luciferase tag luciferase tag 3' range 3' range 3' range 3' range r.759_765delinsagagagg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain TCTCTCC AGAGAGG physical association physical association FIG 4 C D down-regulates quantity by destabilization hsa_mir_185_5p-lncrn_neat1 human long-2 4 7 regulator regulator modulator regulator neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered r.20391_20397delinsagagagg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain TCTCTCC AGAGAGG tag luciferase tag luciferase tag 3' range 3' range 3' range 3' range 8 predetermined predetermined participant predetermined tag luciferase tag visualisation by luciferase assay tag luciferase regulator target regulator target regulator target modulator target neutral component neutral component engineered engineered purified purified physical association physical association FIG 4 C D down-regulates quantity by destabilization hsa_mir_185_5p-lncrn_neat1 human long-1 1 7 regulator regulator modulator regulator neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered r.20391_20397delinsagagagg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain TCTCTCC AGAGAGG tag luciferase tag luciferase tag 3' range 3' range 3' range 3' range 8 predetermined predetermined participant predetermined tag luciferase tag visualisation by luciferase assay tag luciferase regulator target regulator target regulator target modulator target neutral component neutral component engineered engineered purified purified physical association physical association FIG 4 C D down-regulates quantity by destabilization ago2-hsa_mir_185_5p-1 2 9 antibody detection identification by antibody antibody detection qrt-pcr quantitative reverse transcription pcr QRT-PCR unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 7 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level association association Fig 4 E ago2-hsa_mir_185_5p-2 3 9 antibody detection identification by antibody antibody detection qrt-pcr quantitative reverse transcription pcr QRT-PCR unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 7 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level association association Fig 4 E lncrn_neat1 human long-hsa_mir_185_5p-1 5 8 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 7 predetermined predetermined participant predetermined qrt-pcr quantitative reverse transcription pcr QRT-PCR unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell engineered engineered tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined r.20391_20397delinsagagagg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain TCTCTCC AGAGAGG physical association physical association Fig 4 F