DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Identification of an ATP-sensitive potassium channel in mitochondria. Identification of an ATP-sensitive potassium channel in mitochondria. Nature 2019 imex curation Paggio A., Checchetto V., Campo A., Menabò R., Di Marco G., Di Lisa F., Szabo I., Rizzuto R., De Stefani D. rosario.rizzuto@unipd.it, diego.destefani@gmail.com 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 bn-page blue native page bn-page western blot western blot Immuno blot Immuno blot Paggio A., Checchetto V., Campo A., Menabò R., Di Marco G., Di Lisa F., Szabo I., Rizzuto R., De Stefani D. rosario.rizzuto@unipd.it, diego.destefani@gmail.com Accepted 2020-AUG-12 AT 13:15 BST AT 13:15 BST by ORCHARD Nature 2019 imex curation Identification of an ATP-sensitive potassium channel in mitochondria. Identification of an ATP-sensitive potassium channel in mitochondria. Nature 2019 imex curation Paggio A., Checchetto V., Campo A., Menabò R., Di Marco G., Di Lisa F., Szabo I., Rizzuto R., De Stefani D. rosario.rizzuto@unipd.it, diego.destefani@gmail.com 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Paggio A., Checchetto V., Campo A., Menabò R., Di Marco G., Di Lisa F., Szabo I., Rizzuto R., De Stefani D. rosario.rizzuto@unipd.it, diego.destefani@gmail.com Accepted 2020-AUG-12 AT 13:15 BST AT 13:15 BST by ORCHARD Nature 2019 imex curation Identification of an ATP-sensitive potassium channel in mitochondria. Identification of an ATP-sensitive potassium channel in mitochondria. Nature 2019 imex curation Paggio A., Checchetto V., Campo A., Menabò R., Di Marco G., Di Lisa F., Szabo I., Rizzuto R., De Stefani D. rosario.rizzuto@unipd.it, diego.destefani@gmail.com 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-liver Mus musculus liver liver anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Paggio A., Checchetto V., Campo A., Menabò R., Di Marco G., Di Lisa F., Szabo I., Rizzuto R., De Stefani D. rosario.rizzuto@unipd.it, diego.destefani@gmail.com Accepted 2020-AUG-12 AT 13:15 BST AT 13:15 BST by ORCHARD Nature 2019 imex curation Identification of an ATP-sensitive potassium channel in mitochondria. Identification of an ATP-sensitive potassium channel in mitochondria. Nature 2019 imex curation Paggio A., Checchetto V., Campo A., Menabò R., Di Marco G., Di Lisa F., Szabo I., Rizzuto R., De Stefani D. rosario.rizzuto@unipd.it, diego.destefani@gmail.com 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip experimental particp experimental participant identification experimental particp Paggio A., Checchetto V., Campo A., Menabò R., Di Marco G., Di Lisa F., Szabo I., Rizzuto R., De Stefani D. rosario.rizzuto@unipd.it, diego.destefani@gmail.com Accepted 2020-AUG-12 AT 13:15 BST AT 13:15 BST by ORCHARD Nature 2019 imex curation mitok_human Mitochondrial potassium channel CCDC51 Coiled-coil domain-containing protein 51 MITOK protein protein human Homo sapiens Human MMGRSPGFAMQHIVGVPHVLVRRGLLGRDLFMTRTLCSPGPSQPGEKRPEEVALGLHHRLPALGRALGHSIQQRATSTAKTWWDRYEEFVGLNEVREAQGKVTEAEKVFMVARGLVREAREDLEVHQAKLKEVRDRLDRVSREDSQYLELATLEHRMLQEEKRLRTAYLRAEDSEREKFSLFSAAVRESHEKERTRAERTKNWSLIGSVLGALIGVAGSTYVNRVRLQELKALLLEAQKGPVSLQEAIREQASSYSRQQRDLHNLMVDLRGLVHAAGPGQDSGSQAGSPPTRDRDVDVLSAALKEQLSHSRQVHSCLEGLREQLDGLEKTCSQMAGVVQLVKSAAHPGLVEPADGAMPSFLLEQGSMILALSDTEQRLEAQVNRNTIYSTLVTCVTFVATLPVLYMLFKAS D99F1D8AF9EB8C04 mitok_mouse Mitochondrial potassium channel Ccdc51 Mitok Coiled-coil domain-containing protein 51 protein protein mouse Mus musculus Mouse MTGCSPVFAMQHVVGVPRILVRRTFLGTDVTMTRTLCSPGPREKRPEAAALGLFHRLPELGRTLSHTVRHQAASTAKAWWDRYEEFVGLNEVREAQGNVTEAEKVFMVARGLVREAREGLEAQQTKLKEVRDRLDRVSREDNQYLELATLEHRMLQEEKRLRIAYLRAEDSEREKFSLFSAAVRESHEKERTRAERTKNWSLIGSVLGALIGVAGSTYVNRVRLQELKALLLEAQKGPASLQEAIREQASSYSLQQKDLQDLMMDLRGLVHAEQGQGSGSPTGSSTRGKDIDGLSATMKEQLRHSRQVYSCLEGLREQLDGLEKTCSQMAGVLQLAQAPAHPGTVGPVDGALPSSLLEHGSVILALSEMEQRLEAQANRNTVSSTLVTCVTFLATLPLLYMLFKTS D734FCFF6716354D q9nut22 Isoform 2 of ATP-binding cassette sub-family B member 8, mitochondrial ABCB8 MABC1 Short protein protein human Homo sapiens Human MLVHLFRVGIRGGPFPGRLLPPLRFQTFSAVRYSDGYRSSSLLRAVAHLRSQLWAHLPRAPLAPRWSPSAWCWVGGALLGPMVLSKHPHLCLVALCEAEEAPPASSTPHVVGSRFNWKLFWQFLHPHLLVLGVAVVLALGAALVNVQIPLLLGQLVEVVAKYTRDHVGSFMTESQNLSTHLLILYGVQGLLTFGYLVLLSHVGERMAVDMRRALFSSLLRQDITFFDANKTGQLVSRLTTDVQEFKSSFKLVISQGLRSCTQVAGCLVSLSMLSTRLTLLLMVATPALMGVGTLMGSGLRKLSRQCQEQIARAMGVADEALGNVRTVRAFAMEQREEERYGAELEACRCRAEELGRGIALFQGLSNIAFNCMVLGTLFIGGSLVAGQQLTGGDLMSFLVASQTVQRSMANLSVLFGQVVRGLSAGARVFEYMALNPCIPLSGGCCVPKEQLRGSVTFQNVCFSYPCRPGFEVLKDFTLTLPPGKIVALVGQSGGGKTTVASLLERFYDPTAGVVMLDGRDLRTLDPSWLRGQVVGFISQEPVLFGTTIMENIRFGKLEASDEEVYTAAREANAHEFITSFPEGYNTVVGERGTTLSGGQKQRLAIARALIKQPTVLILDEATSALDAESERVVQEALDRASAGRTVLVIAHRLSTVRGAHCIVVMADGRVWEAGTHEELLKKGGLYAELIRRQALDAPRTAAPPPKKPEGPRSHQHKS mitos_human Mitochondrial potassium channel ATP-binding subunit Mitochondrial ATP-binding cassette 1 ABCB8 MABC1 ATP-binding cassette sub-family B member 8, mitochondrial MITOSUR Mitochondrial sulfonylurea-receptor protein protein human Homo sapiens Human MLVHLFRVGIRGGPFPGRLLPPLRFQTFSAVRNTWRNGKTGQLHKAEGEYSDGYRSSSLLRAVAHLRSQLWAHLPRAPLAPRWSPSAWCWVGGALLGPMVLSKHPHLCLVALCEAEEAPPASSTPHVVGSRFNWKLFWQFLHPHLLVLGVAVVLALGAALVNVQIPLLLGQLVEVVAKYTRDHVGSFMTESQNLSTHLLILYGVQGLLTFGYLVLLSHVGERMAVDMRRALFSSLLRQDITFFDANKTGQLVSRLTTDVQEFKSSFKLVISQGLRSCTQVAGCLVSLSMLSTRLTLLLMVATPALMGVGTLMGSGLRKLSRQCQEQIARAMGVADEALGNVRTVRAFAMEQREEERYGAELEACRCRAEELGRGIALFQGLSNIAFNCMVLGTLFIGGSLVAGQQLTGGDLMSFLVASQTVQRSMANLSVLFGQVVRGLSAGARVFEYMALNPCIPLSGGCCVPKEQLRGSVTFQNVCFSYPCRPGFEVLKDFTLTLPPGKIVALVGQSGGGKTTVASLLERFYDPTAGVVMLDGRDLRTLDPSWLRGQVVGFISQEPVLFGTTIMENIRFGKLEASDEEVYTAAREANAHEFITSFPEGYNTVVGERGTTLSGGQKQRLAIARALIKQPTVLILDEATSALDAESERVVQEALDRASAGRTVLVIAHRLSTVRGAHCIVVMADGRVWEAGTHEELLKKGGLYAELIRRQALDAPRTAAPPPKKPEGPRSHQHKS B7E02B063E6C6F3B q3urs9-1 Mitochondrial potassium channel Coiled-coil domain-containing protein 51 Ccdc51 Mitok protein protein mouse Mus musculus Mouse MTGCSPVFAMQHVVGVPRILVRRTFLGTDVTMTRTLCSPGPREKRPEAAALGLFHRLPELGRTLSHTVRHQAASTAKAWWDRYEEFVGLNEVREAQGNVTEAEKVFMVARGLVREAREGLEAQQTKLKEVRDRLDRVSREDNQYLELATLEHRMLQEEKRLRIAYLRAEDSEREKFSLFSAAVRESHEKERTRAERTKNWSLIGSVLGALIGVAGSTYVNRVRLQELKALLLEAQKGPASLQEAIREQASSYSLQQKDLQDLMMDLRGLVHAEQGQGSGSPTGSSTRGKDIDGLSATMKEQLRHSRQVYSCLEGLREQLDGLEKTCSQMAGVLQLAQAPAHPGTVGPVDGALPSSLLEHGSVILALSEMEQRLEAQANRNTVSSTLVTCVTFLATLPLLYMLFKTS D734FCFF6716354D mitos_mouse Mitochondrial potassium channel ATP-binding subunit Abcb8 ATP-binding cassette sub-family B member 8, mitochondrial Mitochondrial sulfonylurea-receptor Mitosur protein protein mouse Mus musculus Mouse MLVHLFRFGIRGGPVPGWSLQSLRFQTFSAARSSDDRLSSHLLRTVAQLRVQLRAHLPRAPPASHWSPSAWCWVGGTLVVPAVLWQHPRLCLIALCEAKESPPAQPTRAPELRFNWKLFWHFLHPHLLALGAAIVLALGAALVNVQIPLLLGQLVEIVAKYTRDHMGSFVSESRKLSVQLLLLYGVQGLLTFGYLVLLSHIGERMAMDMRKALFSSLLRQDIAFFDAKKTGQLVSRLTTDVQEFKSSFKLVISQGLRSCTQVIGSLVSLSMLSPRLTLMLAVVTPALMGVGTLMGSGLRKLSRQCQEQIARATGVADEALGNVRTVRAFAMEKREEERYQAELESCCCKAEELGRGIALFQGLSNIAFNCMVLGTLFIGGSLVAGQQLKGGDLMSFLVASQTVQRSMASLSVLFGQVVRGLSAGARVFEYMALSPVIPLTGGYCIPNKDIRGSITFQNVTFSYPCRPGFNVLKDFTLKLPSGKIVALVGQSGGGKTTVASLLERFYDPEAGSVTLDGHDLRTLNPSWLRGQVIGFISQEPVLFATTIMENIRFGKLDASDEEVYTAAREANAHEFISSFPDGYSTVVGERGTTLSGGQKQRLAIARALIKQPTVLILDEATSALDAESERVVQEALDRASAGRTVLVIAHRLSTVRAAHSIIVMANGQVCEAGTHEELLKKGGLYSELIRRQTLDASLTSTPPAEKPEDPKSCQSKA E573D4276B9658EB abcb8-ccdc51-2 4 9 predetermined predetermined participant predetermined western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey over-expressed over expressed level over-expressed V5 Tag V5 Tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 7 predetermined predetermined participant predetermined anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait over-expressed over expressed level over-expressed Myc Tag Myc Tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range FLAG Tag FLAG Tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q9NUT2-2:p.Lys496Ala K513A mutation mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A Wed Sep 16 10:50:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'Q9NUT2:p.Lys496Ala' human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 physical association physical association Fig3a abcb8-ccdc51-1 1 5 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 8 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 physical association physical association Fig 3b ccdc51-abcb8-2 3 6 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level mouse-liver Mus musculus liver liver 10 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level mouse-liver Mus musculus liver liver physical association physical association Fig 3c ccdc51-abcb8-1 2 5 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 8 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 physical association physical association Ext Data Fig 6b