matrixdb Institut de Biologie et de Chimie des Prot\u00c3\u00a9ines, 7, passage du Vercors 69 367 Lyon cedex 07 FRANCE matrixdb@ibcp.fr http://matrixdb.ibcp.fr Mock publication and experiment for abstract interactions that are not interaction evidences. Mock publication and experiment for abstract interactions that are not interaction evidences. unspecified method Lysyl oxidase-like 2 (LOXL2)-mediated cross-linking of tropoelastin. Lysyl oxidase-like 2 (LOXL2)-mediated cross-linking of tropoelastin. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 imex curation Schmelzer CEH., Heinz A., Troilo H., Lockhart-Cairns MP., Jowitt TA., Marchand MF., Bidault L., Bignon M., Hedtke T., Barret A., McConnell JC., Sherratt MJ., Germain S., Hulmes DJS., Baldock C., Muller L. laurent.muller@college-de-france.fr 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Schmelzer CEH., Heinz A., Troilo H., Lockhart-Cairns MP., Jowitt TA., Marchand MF., Bidault L., Bignon M., Hedtke T., Barret A., McConnell JC., Sherratt MJ., Germain S., Hulmes DJS., Baldock C., Muller L. laurent.muller@college-de-france.fr Accepted 2020-MAR-05 AT 07:59 GMT AT 07:59 GMT by ORCHARD FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Only protein-protein interactions imex curation imex curation Lysyl oxidase-like 2 (LOXL2)-mediated cross-linking of tropoelastin. Lysyl oxidase-like 2 (LOXL2)-mediated cross-linking of tropoelastin. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 imex curation Schmelzer CEH., Heinz A., Troilo H., Lockhart-Cairns MP., Jowitt TA., Marchand MF., Bidault L., Bignon M., Hedtke T., Barret A., McConnell JC., Sherratt MJ., Germain S., Hulmes DJS., Baldock C., Muller L. laurent.muller@college-de-france.fr 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Schmelzer CEH., Heinz A., Troilo H., Lockhart-Cairns MP., Jowitt TA., Marchand MF., Bidault L., Bignon M., Hedtke T., Barret A., McConnell JC., Sherratt MJ., Germain S., Hulmes DJS., Baldock C., Muller L. laurent.muller@college-de-france.fr Accepted 2020-MAR-05 AT 07:59 GMT AT 07:59 GMT by ORCHARD FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Only protein-protein interactions imex curation imex curation Lysyl oxidase-like 2 (LOXL2)-mediated cross-linking of tropoelastin. Lysyl oxidase-like 2 (LOXL2)-mediated cross-linking of tropoelastin. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 imex curation Schmelzer CEH., Heinz A., Troilo H., Lockhart-Cairns MP., Jowitt TA., Marchand MF., Bidault L., Bignon M., Hedtke T., Barret A., McConnell JC., Sherratt MJ., Germain S., Hulmes DJS., Baldock C., Muller L. laurent.muller@college-de-france.fr 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro deamination deamination assay aminase assay deamination predetermined predetermined participant predetermined Schmelzer CEH., Heinz A., Troilo H., Lockhart-Cairns MP., Jowitt TA., Marchand MF., Bidault L., Bignon M., Hedtke T., Barret A., McConnell JC., Sherratt MJ., Germain S., Hulmes DJS., Baldock C., Muller L. laurent.muller@college-de-france.fr Accepted 2020-MAR-05 AT 07:59 GMT AT 07:59 GMT by ORCHARD FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Only protein-protein interactions imex curation imex curation loxl2_human Lysyl oxidase homolog 2 LOXL2 Lysyl oxidase-like protein 2 Lysyl oxidase-related protein 2 Lysyl oxidase-related protein WS9-14 protein protein human Homo sapiens Human MERPLCSHLCSCLAMLALLSPLSLAQYDSWPHYPEYFQQPAPEYHQPQAPANVAKIQLRLAGQKRKHSEGRVEVYYDGQWGTVCDDDFSIHAAHVVCRELGYVEAKSWTASSSYGKGEGPIWLDNLHCTGNEATLAACTSNGWGVTDCKHTEDVGVVCSDKRIPGFKFDNSLINQIENLNIQVEDIRIRAILSTYRKRTPVMEGYVEVKEGKTWKQICDKHWTAKNSRVVCGMFGFPGERTYNTKVYKMFASRRKQRYWPFSMDCTGTEAHISSCKLGPQVSLDPMKNVTCENGLPAVVSCVPGQVFSPDGPSRFRKAYKPEQPLVRLRGGAYIGEGRVEVLKNGEWGTVCDDKWDLVSASVVCRELGFGSAKEAVTGSRLGQGIGPIHLNEIQCTGNEKSIIDCKFNAESQGCNHEEDAGVRCNTPAMGLQKKLRLNGGRNPYEGRVEVLVERNGSLVWGMVCGQNWGIVEAMVVCRQLGLGFASNAFQETWYWHGDVNSNKVVMSGVKCSGTELSLAHCRHDGEDVACPQGGVQYGAGVACSETAPDLVLNAEMVQQTTYLEDRPMFMLQCAMEENCLSASAAQTDPTTGYRRLLRFSSQIHNNGQSDFRPKNGRHAWIWHDCHRHYHSMEVFTHYDLLNLNGTKVAEGHKASFCLEDTECEGDIQKNYECANFGDQGITMGCWDMYRHDIDCQWVDITDVPPGDYLFQVVINPNFEVAESDYSNNIMKCRSRYDGHRIWMYNCHIGGSFSEETEKKFEHFSGLLNNQLSPQ mint 9DF5D25D4824BCCD cadaverine cadaverine 1,5-Diaminopentane 1,5-pentamethylenediamine 1,5-Pentanediamine Cadaverine DAPE Pentamethylenediamine PENTANE-1,5-DIAMINE pentane-1,5-diamine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) bmp1_human Bone morphogenetic protein 1 Procollagen C-proteinase Mammalian tolloid protein BMP1 PCOLC protein protein human Homo sapiens Human MPGVARLPLLLGLLLLPRPGRPLDLADYTYDLAEEDDSEPLNYKDPCKAAAFLGDIALDEEDLRAFQVQQAVDLRRHTARKSSIKAAVPGNTSTPSCQSTNGQPQRGACGRWRGRSRSRRAATSRPERVWPDGVIPFVIGGNFTGSQRAVFRQAMRHWEKHTCVTFLERTDEDSYIVFTYRPCGCCSYVGRRGGGPQAISIGKNCDKFGIVVHELGHVVGFWHEHTRPDRDRHVSIVRENIQPGQEYNFLKMEPQEVESLGETYDFDSIMHYARNTFSRGIFLDTIVPKYEVNGVKPPIGQRTRLSKGDIAQARKLYKCPACGETLQDSTGNFSSPEYPNGYSAHMHCVWRISVTPGEKIILNFTSLDLYRSRLCWYDYVEVRDGFWRKAPLRGRFCGSKLPEPIVSTDSRLWVEFRSSSNWVGKGFFAVYEAICGGDVKKDYGHIQSPNYPDDYRPSKVCIWRIQVSEGFHVGLTFQSFEIERHDSCAYDYLEVRDGHSESSTLIGRYCGYEKPDDIKSTSSRLWLKFVSDGSINKAGFAVNFFKEVDECSRPNRGGCEQRCLNTLGSYKCSCDPGYELAPDKRRCEAACGGFLTKLNGSITSPGWPKEYPPNKNCIWQLVAPTQYRISLQFDFFETEGNDVCKYDFVEVRSGLTADSKLHGKFCGSEKPEVITSQYNNMRVEFKSDNTVSKKGFKAHFFSDKDECSKDNGGCQQDCVNTFGSYECQCRSGFVLHDNKHDCKEAGCDHKVTSTSGTITSPNWPDKYPSKKECTWAISSTPGHRVKLTFMEMDIESQPECAYDHLEVFDGRDAKAPVLGRFCGSKKPEPVLATGSRMFLRFYSDNSVQRKGFQASHATECGGQVRADVKTKDLYSHAQFGDNNYPGGVDCEWVIVAEEGYGVELVFQTFEVEEETDCGYDYMELFDGYDSTAPRLGRYCGSGPPEEVYSAGDSVLVKFHSDDTITKKGFHLRYTSTKFQDTLHSRK DIP protein P13497 original sequence version: 166 DIP protein P13497-1 original sequence version: 130 F89201913AC3CBEA p15502-2 Elastin ELN Tropoelastin protein protein human Homo sapiens Human MAGLTAAAPRPGVLLLLLSILHPSRPGGVPGAIPGGVPGGVFYPGAGLGALGGGALGPGGKPLKPVPGGLAGAGLGAGLGAFPAVTFPGALVPGGVADAAAAYKAAKAGAGLGGVPGVGGLGVSAGAVVPQPGAGVKPGKVPGVGLPGVYPGGVLPGARFPGVGVLPGVPTGAGVKPKAPGVGGAFAGIPGVGPFGGPQPGVPLGYPIKAPKLPGGYGLPYTTGKLPYGYGPGGVAGAAGKAGYPTGTGVGPQAAAAAAAKAAAKFGAGAAGVLPGVGGAGVPGVPGAIPGIGGIAGVGTPAAAAAAAAAAKAAKYGAAAGLVPGGPGFGPGVVGVPGAGVPGVGVPGAGIPVVPGAGIPGAAVPGVVSPEAAAKAAAKAAKYGARPGVGVGGIPTYGVGAGGFPGFGVGVGGIPGVAGVPGVGGVPGVGGVPGVGISPEAQAAAAAKAAKYGVGTPAAAAAKAAAKAAQFGLVPGVGVAPGVGVAPGVGVAPGVGLAPGVGVAPGVGVAPGVGVAPGIGPGGVAAAAKSAAKVAAKAQLRAAAGLGAGIPGLGVGVGVPGLGVGAGVPGLGVGAGVPGFGAVPGALAAAKAAKYGAAVPGVLGGLGALGGVGIPGGVVGAGPAAAAAAAKAAAKAAQFGLVGAAGLGGLGVGGLGVPGVGGLGGIPPAAAAKAAKYGAAGLGGVLGGAGQFPLGGVAARPGFGLSPIFPGGACLGKACGRKRK mint 0A7E4424F9EC0F78 co3a1_human Collagen alpha-1(III) chain COL3A1 protein protein human Homo sapiens Human MMSFVQKGSWLLLALLHPTIILAQQEAVEGGCSHLGQSYADRDVWKPEPCQICVCDSGSVLCDDIICDDQELDCPNPEIPFGECCAVCPQPPTAPTRPPNGQGPQGPKGDPGPPGIPGRNGDPGIPGQPGSPGSPGPPGICESCPTGPQNYSPQYDSYDVKSGVAVGGLAGYPGPAGPPGPPGPPGTSGHPGSPGSPGYQGPPGEPGQAGPSGPPGPPGAIGPSGPAGKDGESGRPGRPGERGLPGPPGIKGPAGIPGFPGMKGHRGFDGRNGEKGETGAPGLKGENGLPGENGAPGPMGPRGAPGERGRPGLPGAAGARGNDGARGSDGQPGPPGPPGTAGFPGSPGAKGEVGPAGSPGSNGAPGQRGEPGPQGHAGAQGPPGPPGINGSPGGKGEMGPAGIPGAPGLMGARGPPGPAGANGAPGLRGGAGEPGKNGAKGEPGPRGERGEAGIPGVPGAKGEDGKDGSPGEPGANGLPGAAGERGAPGFRGPAGPNGIPGEKGPAGERGAPGPAGPRGAAGEPGRDGVPGGPGMRGMPGSPGGPGSDGKPGPPGSQGESGRPGPPGPSGPRGQPGVMGFPGPKGNDGAPGKNGERGGPGGPGPQGPPGKNGETGPQGPPGPTGPGGDKGDTGPPGPQGLQGLPGTGGPPGENGKPGEPGPKGDAGAPGAPGGKGDAGAPGERGPPGLAGAPGLRGGAGPPGPEGGKGAAGPPGPPGAAGTPGLQGMPGERGGLGSPGPKGDKGEPGGPGADGVPGKDGPRGPTGPIGPPGPAGQPGDKGEGGAPGLPGIAGPRGSPGERGETGPPGPAGFPGAPGQNGEPGGKGERGAPGEKGEGGPPGVAGPPGGSGPAGPPGPQGVKGERGSPGGPGAAGFPGARGLPGPPGSNGNPGPPGPSGSPGKDGPPGPAGNTGAPGSPGVSGPKGDAGQPGEKGSPGAQGPPGAPGPLGIAGITGARGLAGPPGMPGPRGSPGPQGVKGESGKPGANGLSGERGPPGPQGLPGLAGTAGEPGRDGNPGSDGLPGRDGSPGGKGDRGENGSPGAPGAPGHPGPPGPVGPAGKSGDRGESGPAGPAGAPGPAGSRGAPGPQGPRGDKGETGERGAAGIKGHRGFPGNPGAPGSPGPAGQQGAIGSPGPAGPRGPVGPSGPPGKDGTSGHPGPIGPPGPRGNRGERGSEGSPGHPGQPGPPGPPGAPGPCCGGVGAAAIAGIGGEKAGGFAPYYGDEPMDFKINTDEIMTSLKSVNGQIESLISPDGSRKNPARNCRDLKFCHPELKSGEYWVDPNQGCKLDAIKVFCNMETGETCISANPLNVPRKHWWTDSSAEKKHVWFGESMDGGFQFSYGNPELPEDVLDVHLAFLRLLSSRASQNITYHCKNSIAYMDQASGNVKKALKLMGSNEGEFKAEGNSKFTYTVLEDGCTKHTGEWSKTVFEYRTRKAVRLPIVDIAPYDIGGPDQEFGVDVGPVCFL B904B4E05E17D339 eln-loxl2-1 3 8 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role bait bait purified purified Expression tag His tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Tag Myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. direct interaction direct interaction Figure 5A, 5B eln-loxl2-2 4 8 enzyme target enzyme target substrate substrate neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 5 enzyme enzyme neutral component neutral component Expression tag His tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Expression tag Myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. direct interaction direct interaction Figure 6 A, B, C, D, Figure S3 cadaverine-loxl2-1 4 5 enzyme enzyme neutral component neutral component purified purified Expression tag His tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Expression tag Myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 6 enzyme target enzyme target substrate substrate neutral component neutral component purified purified direct interaction direct interaction Figure 6A bmp1-6 2 27 enzyme target enzyme target substrate substrate neutral component neutral component purified purified human-293 ebna Homo sapiens 293 ebna 293 ebna 7 enzyme enzyme neutral component neutral component purified purified Expression tag Flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 ebna Homo sapiens 293 ebna 293 ebna direct interaction direct interaction Figure S1B collagen_iii_human Type III collagen Collagen type III trimer Type III collagen COL3A1 trimer 9 unspecified role unspecified role direct interaction direct interaction human Homo sapiens Human stable complex stable complex physical interaction evidence used in manual assertion physical interaction evidence used in manual assertion IPI inferred from physical interaction Occurs in most soft connective tissues. Bonded to type I collagen (CPX-1650) by covalent lysine-derived cross-links. Homotrimer Triple-helices consisting of about 1000 amino acids per chain forming a coiled coil structure. Each polypeptide forms a left-handed helix in which every third residue, glycine, comes into the center of the superhelix. Propeptides (telopeptides) at both ends of the molecular precursor undergo highly complex fibrillogenesis. created with intact-jami-dbscripts