IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ FOXP3 and miR-155 cooperate to control the invasive potential of human breast cancer cells by down regulating ZEB2 independently of ZEB1. FOXP3 and miR-155 cooperate to control the invasive potential of human breast cancer cells by down regulating ZEB2 independently of ZEB1. Oncotarget 2018 imex curation Brown CY., Dayan S., Wong SW., Kaczmarek A., Hope CM., Pederson SM., Arnet V., Goodall GJ., Russell D., Sadlon TJ., Barry SC. simon.barry@adelaide.edu.au 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-bt_549 Homo sapiens BT_549 Breast ductal carcinoma cell line bt_549 BT 549 Human Breast ductal carcinoma cell line ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Brown CY., Dayan S., Wong SW., Kaczmarek A., Hope CM., Pederson SM., Arnet V., Goodall GJ., Russell D., Sadlon TJ., Barry SC. simon.barry@adelaide.edu.au Accepted 2020-MAR-13 AT 08:10 GMT AT 08:10 GMT by PPORRAS Oncotarget 2018 imex curation FOXP3 and miR-155 cooperate to control the invasive potential of human breast cancer cells by down regulating ZEB2 independently of ZEB1. FOXP3 and miR-155 cooperate to control the invasive potential of human breast cancer cells by down regulating ZEB2 independently of ZEB1. Oncotarget 2018 imex curation Brown CY., Dayan S., Wong SW., Kaczmarek A., Hope CM., Pederson SM., Arnet V., Goodall GJ., Russell D., Sadlon TJ., Barry SC. simon.barry@adelaide.edu.au 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag luciferase tag visualisation by luciferase assay tag luciferase Brown CY., Dayan S., Wong SW., Kaczmarek A., Hope CM., Pederson SM., Arnet V., Goodall GJ., Russell D., Sadlon TJ., Barry SC. simon.barry@adelaide.edu.au Accepted 2020-MAR-13 AT 08:10 GMT AT 08:10 GMT by PPORRAS Oncotarget 2018 imex curation foxp3_human Forkhead box protein P3 Scurfin FOXP3 IPEX JM2 protein protein human Homo sapiens Human MPNPRPGKPSAPSLALGPSPGASPSWRAAPKASDLLGARGPGGTFQGRDLRGGAHASSSSLNPMPPSQLQLPTLPLVMVAPSGARLGPLPHLQALLQDRPHFMHQLSTVDAHARTPVLQVHPLESPAMISLTPPTTATGVFSLKARPGLPPGINVASLEWVSREPALLCTFPNPSAPRKDSTLSAVPQSSYPLLANGVCKWPGCEKVFEEPEDFLKHCQADHLLDEKGRAQCLLQREMVQSLEQQLVLEKEKLSAMQAHLAGKMALTKASSVASSDKGSCCIVAAGSQGPVVPAWSGPREAPDSLFAVRRHLWGSHGNSTFPEFLHNMDYFKFHNMRPPFTYATLIRWAILEAPEKQRTLNEIYHWFTRMFAFFRNHPATWKNAIRHNLSLHKCFVRVESEKGAVWTVDELEFRKKRSQRPSRCSNPTPGP DIP protein Q9BZS1 original sequence version: 139 DIP protein Q9BZS1 original sequence version: 131 91737C3CEA665A15 mrna_zeb2 zinc finger E-box binding homeobox 2 mrna messenger rna mRNA mrna human Homo sapiens Human AGTTCTAACCTGCTCTGCAGGAATAACGGTCCTGCCTCCCGACACTCTTGGCGAGGTTTTTGTACAGTTTGCTCCGGGAGCTGTTTCTTCGCTTCCACCTTTTTCTCCCCCACACTTCGCGGCTTCTTCATGCTTTTTCTTCTCACCATTTCTGGCCAAAACTACAAACAAGACTTCGCAGATCGAGCCTGCGTGCTGCCGAAGCAGGGCGCCGAGTCCATGCGAACTGCCATCTGATCCGCTCTTATCAATGAAGCAGCCGATCATGGCGGATGGCCCCCGGTGCAAGAGGCGCAAACAAGCCAATCCCAGGAGGAAAAACGTGGTGAACTATGACAATGTAGTGGACACAGGTTCTGAAACAGATGAGGAAGACAAGCTTCATATTGCTGAGGATGACGGTATTGCCAACCCTCTGGACCAGGAGACGAGTCCAGCTAGTGTGCCCAACCATGAGTCCTCCCCACACGTGAGCCAAGCTCTGTTGCCAAGAGAGGAAGAGGAAGATGAAATAAGGGAGGGTGGAGTGGAACACCCCTGGCACAACAACGAGATTCTACAAGCCTCTGTAGATGGTCCAGAAGAAATGAAGGAAGACTATGACACTATGGGGCCAGAAGCCACGATCCAGACCGCAATTAACAATGGTACAGTGAAGAATGCAAATTGCACATCAGATTTTGAGGAATACTTTGCCAAAAGAAAACTGGAGGAACGCGATGGTCATGCAGTCAGCATCGAGGAGTACCTTCAGCGCAGTGACACAGCCATTATTTACCCAGAAGCCCCTGAGGAGCTGTCTCGCCTTGGCACGCCAGAGGCCAATGGGCAAGAAGAAAATGACCTGCCACCTGGAACTCCAGATGCTTTTGCCCAACTGCTGACCTGCCCCTACTGCGACCGGGGCTACAAGCGCTTGACATCACTGAAGGAGCACATCAAGTACCGCCACGAGAAGAATGAAGAGAACTTTTCCTGCCCTCTCTGTAGCTACACGTTTGCCTACCGCACCCAGCTCGAGCGGCATATGGTGACACACAAGCCAGGGACAGATCAGCACCAAATGCTAACCCAAGGAGCAGGTAATCGCAAGTTCAAATGCACAGAGTGTGGCAAGGCCTTCAAATATAAACACCATCTGAAAGAACACCTGCGAATTCACAGTGGTGAAAAACCTTACGAGTGCCCAAACTGCAAGAAACGTTTCTCCCATTCTGGTTCCTACAGTTCGCACATCAGCAGCAAGAAATGTATTGGTTTAATCTCTGTAAATGGCCGAATGAGAAACAATATCAAGACGGGTTCTTCCCCTAATTCTGTTTCTTCTTCTCCTACTAATTCAGCCATTACCCAGTTAAGAAACAAGTTGGAGAATGGAAAACCACTTAGTATGTCTGAACAGACAGGCTTACTTAAAATTAAAACAGAACCACTAGACTTCAATGACTATAAAGTTCTTATGGCTACACACGGGTTTAGTGGCACTAGTCCCTTTATGAATGGTGGGCTTGGAGCCACCAGCCCTTTAGGAGTTCATCCATCTGCTCAGAGTCCAATGCAGCACTTAGGTGTAGGGATGGAAGCCCCTTTACTTGGGTTTCCCACCATGAATAGTAATTTAAGTGAGGTACAAAAGGTTCTACAGATTGTGGACAATACTGTTTCCAGGCAAAAAATGGACTGCAAGGCTGAAGAAATTTCAAAGTTGAAAGGTTATCACATGAAGGATCCATGCTCTCAACCTGAGGAACAAGGAGTTACTTCTCCTAATATTCCGCCTGTCGGTCTTCCGGTAGTGAGTCATAATGGTGCCACTAAAAGTATTATTGACTATACGTTGGAAAAAGTCAATGAAGCCAAAGCTTGCCTCCAGAGCTTGACTACTGACTCAAGGAGACAGATCAGTAATATAAAGAAAGAGAAGCTACGTACTTTAATAGATTTGGTCACTGATGACAAAATGATTGAGAACCACAACATATCCACTCCATTTTCATGCCAGTTCTGTAAAGAAAGTTTTCCTGGCCCCATCCCTTTGCATCAGCATGAACGTTACCTTTGTAAGATGAATGAAGAGATCAAGGCGGTCCTGCAGCCTCATGAAAACATAGTCCCCAACAAAGCCGGAGTTTTTGTTGATAATAAAGCCCTCCTCTTGTCATCTGTACTTTCTGAGAAAGGAATGACAAGCCCCATCAACCCATACAAGGACCACATGTCTGTACTCAAAGCATACTATGCTATGAACATGGAGCCCAACTCCGATGAACTGCTGAAAATTTCCATTGCTGTGGGCCTTCCTCAGGAATTTGTGAAGGAATGGTTTGAACAACGAAAAGTCTACCAGTACTCAAATTCCAGGTCCCCATCCCTGGAAAGAAGCTCCAAGCCGTTAGCTCCCAACAGTAACCCTCCCACAAAAGACTCTTTATTACCCAGGTCTCCTGTAAAACCTATGGACTCCATAACATCACCATCTATAGCAGAACTCCACAACAGTGTTACGAATTGTGATCCTCCTCTCAGGCTAACAAAACCTTCCCATTTTACCAATATTAAACCAGTTGAAAAATTGGACCACTCCAGGAGTAATACTCCTTCTCCCTTAAATCTTTCCTCCACATCTTCTAAAAACTCCCACAGTAGTTCATACACTCCAAACAGCTTCTCTTCTGAGGAGCTCCAGGCTGAGCCTTTAGACTTGTCATTACCAAAACAAATGAAAGAACCCAAAAGTATTATAGCCACAAAGAACAAAACAAAAGCTAGTAGCATCAGTTTAGATCATAACAGTGTTTCTTCCTCATCTGAAAACTCAGATGAGCCTCTGAACTTGACTTTTATCAAGAAGGAATTTTCAAATTCAAATAATCTGGACAACAAAAGCACTAACCCAGTGTTCAGCATGAACCCATTTAGTGCCAAACCTTTATACACAGCTCTTCCACCTCAAAGCGCATTTCCCCCTGCTACTTTCATGCCACCAGTCCAGACCAGTATTCCTGGGCTACGACCATACCCAGGACTGGATCAGATGAGCTTCCTACCACATATGGCCTACACCTACCCAACTGGAGCAGCTACTTTTGCTGATATGCAGCAAAGGAGAAAGTACCAGCGGAAACAAGGATTTCAGGGAGAATTGCTTGATGGAGCACAAGACTACATGTCAGGCCTAGATGATATGACAGACTCCGACTCCTGTCTGTCTCGCAAAAAGATCAAGAAGACAGAGAGTGGCATGTATGCATGTGACTTATGTGACAAGACATTCCAGAAAAGCAGTTCCCTTCTGCGACATAAATACGAACACACAGGAAAAAGACCACATCAGTGTCAGATTTGTAAGAAAGCGTTTAAACACAAGCACCACCTTATCGAGCACTCAAGGCTTCACTCGGGCGAGAAGCCCTATCAGTGTGATAAATGTGGCAAGCGCTTCTCACACTCGGGCTCGTACTCGCAGCACATGAATCACAGGTATTCCTACTGCAAGCGGGAGGCGGAGGAGCGGGAAGCGGCGGAGCGCGAGGCGCGCGAGAAAGGGCACTTGGAACCCACCGAGCTGCTGATGAACCGGGCTTACTTGCAGAGCATTACCCCTCAGGGGTACTCTGACTCGGAGGAGAGGGAGAGTATGCCGAGGGATGGCGAGAGCGAGAAGGAGCACGAGAAAGAAGGCGAGGATGGCTACGGGAAGCTGGGCAGACAGGATGGCGACGAGGAGTTCGAGGAGGAAGAGGAAGAAAGTGAAAATAAAAGTATGGATACGGATCCCGAAACGATACGAGATGAAGAAGAGACTGGAGATCACTCCATGGACGATAGTTCGGAGGATGGGAAAATGGAAACCAAATCAGACCACGAGGAAGACAATATGGAAGATGGCATGTAATAAACTACTGCATTTTAAGCTTCCTATTTTTTTTTCCAGTAGTATTGTTACCTGCTTGAAAACACTGCTGTGTTAAGCTGTTCATGCACGTGCCTGACGCTTCCAGGAAGCTGTAGAGAGGGACAGAAGGGGCGGTTCAGCCAAGACAGATGTAGACGGAGTTGGAGCTGGGTATTGTTAAAAACTGCATTATGCAAAAATTTTGTACAGTGTTAAGGCCTAAAAACTGTGTGGTTCAGAGACTAATTCCTGTGTTTAATAGCATTTATACTTTAAGCACAACTAGAAAATTGTAAGAATTGCACTCTACTTATGTATCACTACAAACTTTAAAAAACTATGTCTAATTTATATTAATACATTTTAAAAAGGTGCCCGCACTACCATACATCAGTATTTTTATTATTATTATTGTTATTCCTTTTTAATTTAATGTGCTCGCACTACAATGCATCAGTATTATGATTCCTCTGTACTTTCCTTTCGCTATTCATCAATTTCCCATTTTTTTTTTCAGCTTAAGTAACCACACAATTTTAGGCCTCAATTTTTTTTTTTTTCTGTGAAGGAACTTGAAGTGATGCATGTGTGAATTTAAGATACCGAAGTCTTAAAGTGACCTGGACGTGAAGGAAAAAGTAAGATGAGAAATAAAGAAAGCCTTTGTAAGGTGGTTTTAAAAGCCTTATATGCAAACCTTTTAATCTGTGTTTCTGCAAGTGCCATCCTTGTACAGTGTTAAGAGGGTAACATGGGTTACCTTTGCACCAGCTTCAGTGTTAAGCTCACCCTGTTCTTTGAAGCACCCATGTCAGTATTAGAAGAATAGGCAGCAGTTCCTTAGTTTACATATGTTTGTGCAATTATTTTCTGTACTTTTTTGTTCATTAATTTTGTCAGTATTACACCAAACTGTTTTTGCAACAAAAAAATTTTTTTTGCATTCATTTAATTTTAGGTCAAATAACATTTTATTTATGTGGCTCATTTTATATTTCCTAATTTTATTTATTTCATACTGTAGTGTACAGTATTATAGTTCTTCAATATATAGATATATTTTAGTAAAAAAGGAACATGACGTTGATCATTTGGGCAAATTTTACGTAAAGAGAAGAGCATTTATTGTGTTTTGGAACATTAATTGTGAGATGGGATTTTTCAATTTTATTATTTTATTTTTGTTTTTTTCCAATTACTGGAAATTCCAAATTTGGGAACTTTTGATACGATCTTGTGAAAACACTGTATTTTCGACTGAAAATTCCACTTTCTTCATCTTGTTTTTTAGCTAAAAAGAGGGACTGTTAAATACAATGTATGATACCATGACAAAAATCTTTCCTGAATTGTCTTTGTAAAAGTATTATTGAATTTTCAATTTGTAATTTCTTTTGAAAATGACCATGCTCGAATAAAAATGTAGCCAAACTAAGAATGTAGTTAATGAGTTCTGTACTTTTAGAGAGTTTTCCTTCAATGACCATTAACATGTAACATGCTTTATGCTTATAATAATGCTAATTATGTTTTTTTCATATAATTTTAGTTTAGCAATAATTTTGACTGGTACCAATAACTGTTTTTTAAAATTCCATACCTATGTACAGCAATTTTACAGCTTTTCTCAACTGATCCTGATTCCAGATTGTGTATTTTTATGTGAGGTTATATTATTCAAATTTAGTCTATTTACTTTACAGACATTTCTACTTTTGCATTACGAGTATTTAGAGATTATGTGTTAAAAATTCACTTCTCTGTCCAAGGGGTCTTTGTGATTTATTCAAAAAAAAGTCTAATTTCAAAAAGACAGCTATTATTCAGTGTTATTTATAATATGTAACCTTTTTTAAAGGATTGGGATAGTTTATCTCACTTTTTGAAATGCAGACAGTAGTTTACCGTTTATCTGAAACTAGAAGGCGTGGGTGGGAGAGGAAAAGCTAAAAGCAAATGCTAACAAAAATAACCGTGATTTTCTAAGACAGTTTTTCAGTTTTTACAAGATGACCCTAATATTCAGAATATGAATGTATTCGTAGGTTTTACATAATGACTTTTATCAAGAAACTAGATTCTGCTTCTTAAATCTAATTGCCAAGTGAAGAATAACAGAAAAAACAGATTACCTTATCAAATTTACAGCTCTTGAATATACAGAACTATAATATAGTAGCTGTCCATGTATTTTTTCTACTTTAGAATCAAAGAAGAAAAGCATCATTTTGCTATTAAATTTGCTAAAATTTTGAGTATGATATTTCCAGTTGGCAAGAACAACATATTTATATTTATTCCTTAGCCATAATACCACTTTCCTAAATTTCACAAAAGTCATTCTTTGCAACTTGAAACTCAATAGAAAGTGTGTATGTGTGTGTGTGTATATATATATATATATACACACACACACATACACAGAAAGGATGTAATGAAGATACAGTAATAGTTGAGCAGACCTTTTTAGAAAAACATGTTTTTAGCTCTATCTTCAAACTTTCTGGCAGAGGGGGTGGGGGGGGCAGGGGGAGGAGTGGCATCAAAATGCTATGCCTCCTGTTATCCACAGCCTAGAGTTTTTATATTTGGAAAGTTTAGAAAATTCTATCCTCGTTTCTCCTTCTTTGAATGGCACAAATAAATACACTACATAAATTTTTCTGGTTTGAAAGGCTCTAGGCGATAACTTTATTAATTCAACCTGAAAATATCAAGCCATTAAATTTTGTCCGGGTAGAATAAATCCCTGTGGCCTCTTTTAAAGCAATGTAGGTCTCTGTTGCCCATGGGGCATATCTGTGTCCCAATCCACAAGAGATAGGACCAACAAACAATGAATGTGCAACCTAACTCTTTCTCCTTGGAAAGAAGAAAGTGTGCACGAAGTAGAGGAGGGTGGGCAGACCCTGCCTTGCCCCTCCTGTTACCCCCTTCTCTGTCATTTGTTCCTAACTCCATTTCATAGGCAGGCTCAGAATACCTGAGTCTGAAAATATCAGGATAACACTTGTGAATTGTGACAATCACTACAATGTCCCATATCTGAGGAGTTTTTTTTAATGCTATTTATCCGCTGGACACGATTGCACATTAGGGCTGCATAATCCTCTAACTCTAGGGAAAAATAAAAACTTTTGATTTGTCTTAAGATTCTTCTCCAAGGTCGCAAACAAGAAATTCCCCTCCACAACCAAGAGATGTGCATTTTAGTAACATCAGATGTGTTCTTCTGTTTTATCAACTACTTACTCTTCCCACACGCTTAGTTCTAAATCTAACCTTTCCCCCCTCGAATAGGGGGCAGGGGAGGATGAGGAAACACTGGAACAACTGAACACCCCTGCCCATTTTCTCCAAGAGCCTTTTGTATTCTAGCATATCTGTGCAATCTTTTCTTTTTTCTTCACATGACACTGTAAGCTTAGGCCTGAAATAACTGGGAAGAGAGATGCGTATCAGAATTTCTCCGCAAGAGCTAAACAAAACATACATCTTCCTTAGCATGAATTGGACTGGGGGCGGAGTGGGAGGGCTTGGAGGAAAGGGGAAAGAAGGGACTATATTTGAATAAATATGAATAAATGTATTAGATACTTTTCACAATCAGATAACTTTTAAAAAGGTCATTTTTTATCTTTCTAATAATGTAAGCCTTAATAAAAGCAAATCTTAGTCACAAATTTGAGGAGACTGCCCAATAATAAGTTTACATGTATTTGAACTGAAAAATTGTTAACCATGCTTTTGCTCCAAGATGTGTGAGGCCATTCAGGGGCTGTAGGGCCCTGGATATACACACAAACAAGTGTGTGTATATCTGGAGCCCCACACATTGTAATAAACACAGCTGCATTTATTTGACTATGTGATCCCATGTACATGTAAAAACATTCAAACAAACACACTCAGCGGATTTATTTATTGTGCAATGGGGCAATTATTCAAATAAACATGCTCAATGCAATTATTTGAATCTCACATTGCATGTTCATCAATCATAGCACTAAAAAAAGAGGGGGAAAAAACACCAAAGAATTCACATGGGGAAAAAATATATATATGAAAACCACCTTATTATAGATTTTATAGGGCAGCTGAGGTTATGGCTCCCTTCTTAACTGTAACTCAACTATTCTGTATTCAATGACATTTGTTTCTAATGATTAATTGGTTCACTCACTTGATCATATAATAGCAAACTTTATAAACCTGTATTGTGTAGAGATGTGAAATCTCTATATTTCAAGAGCAGAAGAGTTCTTTCTAGACACCTTACATCAAGGGACACTGGTCCAATTATTATCGCTTATATAAGCACTCCTATAAATTCTGAAAAATTTTATACATGCAACAAAACATTCCTACATTTGAAGACATTAAGAAAAATCACAGGTGACTCATCTGATCATTCTATATATTAATAAATATTATGACATATATGTGAACACATCACAAATCATATTGGTGTACCAAGAGGCAATTTATGCCTCTCTTAAGTATGTACTGACATAACCTAATATACTAAAATGGGAAGGGGCTTTTAGTCACTGAAATATGCATCGTGTAACAAAGATGAAGAAAATACATGGCTTGTGCCCATCATAAAAAAAGATTCAGACTGAAGGCTTAGCTTTGGTTTTTTCAATTAAATTGTTAAACTGTGCACAGTGATTTTTTTTTAGAACTTGAGACATTTGTGATGTTGGCTGTTTAAATCTTTGTTACCTTCGCTGTGAATTGAAATTGTACATATTTAGTAAATCATGCAGACAAAACAAACTTTTTAGACAATATTTTTATTGGAGAGTTTTCTTTTCCTGTATCCATGTTAAAAAAAAAAAAGACCTCCTTTCCCAAAATAAAAATGTCAATACTAAATTTAAAGAAGTATAAAGGAATGATTGCTTCCTTTAGAGCAAAATATTTAAATAAACATGGAGATAATTGGCAACATGTTCTTTTTGGGCTAGTAGGCTGTGTCCAATTTTTTGGGTCTGATGTTTCAGAGGGCCTCTGTTTCAGGGTTGAAGATGATATATTAATCTCGGAATTAAACAAATGCTATTAAATAACAGAAATAAATCCTTCAGTCTTCATTTGTATCTGAAATGTGGAATTAGTCAGAGAAATGGCACCGAGATAATATGATGCTGAATATGCCACAAGCTAAAACTTTCTCTATTAAACGAGTCGCCTACTACCTATAATCTAGCATGACATGTTAAAATGCTCCAAAATACTGAAAATAGAAAATGAACACTACTGTTCCGATTTGGTCTTGAGATTAATATGACAAAGATTCTAATAATTTTAGTCAAAGAAAATGATGAGTAAAAAATACATGAAGATTAAAGTGATGACAAATGA zeb2_human_gene Human ZFHX1B gene, promoter gene gene human Homo sapiens Human hsa-mir-155-5p microrna hsa-miR-155 5p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA human Homo sapiens Human UUAAUGCUAAUCGUGAUAGGGGU hsa_mir_155_5p-mrna_zeb2-1 2 6 predetermined predetermined participant predetermined tag luciferase tag visualisation by luciferase assay tag luciferase regulator regulator modulator regulator neutral component neutral component engineered engineered purified purified 4 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered r.4156_4161delinsaagctt mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain AGCATT AAGCTT r.4082_4087delinstgtaca mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain GCATTA TGTACA region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain tag luciferase tag luciferase tag 3' range 3' range 3' range 3' range physical association physical association Fig 3 C, D, E zeb2_human_gene-1 1 3 antibody detection identification by antibody antibody detection primer specific pcr primer specific pcr unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell engineered engineered FP3 binding region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 2:144452709-144452840 FP3_BR_F:GTTTTTCCTTCCAGCCTTTTTCCT FP3_BR_R:TCCCCACGTAAATGTTTTGCTTTT physical association physical association Fig1B