DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Cryo-EM structure of human rhodopsin bound to an inhibitory G protein. Cryo-EM structure of human rhodopsin bound to an inhibitory G protein. Nature 2018 imex curation Kang Y., Kuybeda O., de Waal PW., Mukherjee S., Van Eps N., Dutka P., Zhou XE., Bartesaghi A., Erramilli S., Morizumi T., Gu X., Yin Y., Liu P., Jiang Y., Meng X., Zhao G., Melcher K., Ernst OP., Kossiakoff AA., Subramaniam S., Xu HE. eric.xu@vai.org 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by staining molecular weight estimation by staining weight by staining Kang Y., Kuybeda O., de Waal PW., Mukherjee S., Van Eps N., Dutka P., Zhou XE., Bartesaghi A., Erramilli S., Morizumi T., Gu X., Yin Y., Liu P., Jiang Y., Meng X., Zhao G., Melcher K., Ernst OP., Kossiakoff AA., Subramaniam S., Xu HE. eric.xu@vai.org Accepted 2021-APR-19 AT 14:36 BST AT 14:36 BST by PPORRAS Nature 2018 Only protein-protein interactions imex curation imex curation Cryo-EM structure of human rhodopsin bound to an inhibitory G protein. Cryo-EM structure of human rhodopsin bound to an inhibitory G protein. Nature 2018 imex curation Kang Y., Kuybeda O., de Waal PW., Mukherjee S., Van Eps N., Dutka P., Zhou XE., Bartesaghi A., Erramilli S., Morizumi T., Gu X., Yin Y., Liu P., Jiang Y., Meng X., Zhao G., Melcher K., Ernst OP., Kossiakoff AA., Subramaniam S., Xu HE. eric.xu@vai.org 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog experimental particp experimental participant identification experimental particp Kang Y., Kuybeda O., de Waal PW., Mukherjee S., Van Eps N., Dutka P., Zhou XE., Bartesaghi A., Erramilli S., Morizumi T., Gu X., Yin Y., Liu P., Jiang Y., Meng X., Zhao G., Melcher K., Ernst OP., Kossiakoff AA., Subramaniam S., Xu HE. eric.xu@vai.org Accepted 2021-APR-19 AT 14:36 BST AT 14:36 BST by PPORRAS Nature 2018 Only protein-protein interactions imex curation imex curation fab_lc_synthetic Synthetic Fab light chain protein protein chemical synthesis Chemical synthesis (Chemical synthesis) SDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQSSSSLITFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC c562_ecolx Soluble cytochrome b562 cybC protein protein ecolx Escherichia coli MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYHQKYR D7D822AC8FEED751 gbb1_rat Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Transducin beta chain 1 Gnb1 protein protein rat Rattus norvegicus (Rat) Rat MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN DIP protein P54311 original sequence version: 123 DIP protein P54311 original sequence version: 96 896CBD32D2686598 fab_hc_synthetic Synthetic Fab Heavy chain protein protein chemical synthesis Chemical synthesis (Chemical synthesis) EISEVQLVESGGGLVQPGGSLRLSCAASGFNFYYSSIHWVRQAPGKGLEWVASIYSYSGSTSYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARYPWYWWMEKPYLSLYGMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT gbg2_bovin Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 GNG2 G gamma-I protein protein bovin Bos taurus (Bovine) Bovine MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFREKKFFCAIL DIP protein P63212 original sequence version: 54 DIP protein P63212 original sequence version: 55 DIP protein P63212 original sequence version: 66 EDB74E4135E7A37A gnai1_human Guanine nucleotide-binding protein G(i) subunit alpha-1 GNAI1 Adenylate cyclase-inhibiting G alpha protein protein protein human Homo sapiens Human MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF 9F88311B46E62DE3 cybc-gnai1-1 2 8 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified HIS tag HIS tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range MBP tag MBP tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 6 unspecified role unspecified role neutral component neutral component purified purified baclat1825 Bacteria Latreille et al. 1825 3 unspecified role unspecified role neutral component neutral component purified purified baclat1825 Bacteria Latreille et al. 1825 4 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 7 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified HIS tag HIS tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range MBP tag MBP tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 5 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association Fig 2, Extended Data Fig 2, Extended Data Table 1 cybc-gnai1-2 1 8 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role neutral component neutral component purified purified spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 6 unspecified role unspecified role neutral component neutral component purified purified baclat1825 Bacteria Latreille et al. 1825 3 unspecified role unspecified role neutral component neutral component purified purified baclat1825 Bacteria Latreille et al. 1825 4 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role neutral component neutral component purified purified spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 7 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role neutral component neutral component purified purified spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 5 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role neutral component neutral component purified purified spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association Extended Data Fig 1a, b