IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Cooperative Repression of Insulin-Like Growth Factor Type 2 Receptor Translation by MicroRNA 195 and RNA-Binding Protein CUGBP1. Cooperative Repression of Insulin-Like Growth Factor Type 2 Receptor Translation by MicroRNA 195 and RNA-Binding Protein CUGBP1. Molecular and cellular biology 2017 imex curation Zhang Y., Zhang Y., Xiao L., Yu TX., Li JZ., Rao JN., Turner DJ., Gorospe M., Wang JY., Zhang Y., Zhang Y., Xiao L., Yu TX., Li JZ., Rao JN., Turner DJ., Gorospe M., Wang JY. jywang@som.umaryland.edu 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation rat-iec6 Rat small intestine cell iec6 Rat small intestine cell pull down pull down qrt-pcr quantitative reverse transcription pcr QRT-PCR Zhang Y., Zhang Y., Xiao L., Yu TX., Li JZ., Rao JN., Turner DJ., Gorospe M., Wang JY., Zhang Y., Zhang Y., Xiao L., Yu TX., Li JZ., Rao JN., Turner DJ., Gorospe M., Wang JY. jywang@som.umaryland.edu Accepted 2020-NOV-25 AT 08:05 GMT AT 08:05 GMT by ORCHARD Molecular and cellular biology 2017 imex curation Cooperative Repression of Insulin-Like Growth Factor Type 2 Receptor Translation by MicroRNA 195 and RNA-Binding Protein CUGBP1. Cooperative Repression of Insulin-Like Growth Factor Type 2 Receptor Translation by MicroRNA 195 and RNA-Binding Protein CUGBP1. Molecular and cellular biology 2017 imex curation Zhang Y., Zhang Y., Xiao L., Yu TX., Li JZ., Rao JN., Turner DJ., Gorospe M., Wang JY., Zhang Y., Zhang Y., Xiao L., Yu TX., Li JZ., Rao JN., Turner DJ., Gorospe M., Wang JY. jywang@som.umaryland.edu 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation rat-iec6 Rat small intestine cell iec6 Rat small intestine cell mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag luciferase tag visualisation by luciferase assay tag luciferase Zhang Y., Zhang Y., Xiao L., Yu TX., Li JZ., Rao JN., Turner DJ., Gorospe M., Wang JY., Zhang Y., Zhang Y., Xiao L., Yu TX., Li JZ., Rao JN., Turner DJ., Gorospe M., Wang JY. jywang@som.umaryland.edu Accepted 2020-NOV-25 AT 08:05 GMT AT 08:05 GMT by ORCHARD Molecular and cellular biology 2017 imex curation Cooperative Repression of Insulin-Like Growth Factor Type 2 Receptor Translation by MicroRNA 195 and RNA-Binding Protein CUGBP1. Cooperative Repression of Insulin-Like Growth Factor Type 2 Receptor Translation by MicroRNA 195 and RNA-Binding Protein CUGBP1. Molecular and cellular biology 2017 imex curation Zhang Y., Zhang Y., Xiao L., Yu TX., Li JZ., Rao JN., Turner DJ., Gorospe M., Wang JY., Zhang Y., Zhang Y., Xiao L., Yu TX., Li JZ., Rao JN., Turner DJ., Gorospe M., Wang JY. jywang@som.umaryland.edu 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation rat-iec18 Rattus norvegicus iec18 iec18 rna-ip rna immunoprecipitation rna-ip rt-pcr reverse transcription pcr RT-PCR Zhang Y., Zhang Y., Xiao L., Yu TX., Li JZ., Rao JN., Turner DJ., Gorospe M., Wang JY., Zhang Y., Zhang Y., Xiao L., Yu TX., Li JZ., Rao JN., Turner DJ., Gorospe M., Wang JY. jywang@som.umaryland.edu Accepted 2020-NOV-25 AT 08:05 GMT AT 08:05 GMT by ORCHARD Molecular and cellular biology 2017 imex curation Cooperative Repression of Insulin-Like Growth Factor Type 2 Receptor Translation by MicroRNA 195 and RNA-Binding Protein CUGBP1. Cooperative Repression of Insulin-Like Growth Factor Type 2 Receptor Translation by MicroRNA 195 and RNA-Binding Protein CUGBP1. Molecular and cellular biology 2017 imex curation Zhang Y., Zhang Y., Xiao L., Yu TX., Li JZ., Rao JN., Turner DJ., Gorospe M., Wang JY., Zhang Y., Zhang Y., Xiao L., Yu TX., Li JZ., Rao JN., Turner DJ., Gorospe M., Wang JY. jywang@som.umaryland.edu 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Zhang Y., Zhang Y., Xiao L., Yu TX., Li JZ., Rao JN., Turner DJ., Gorospe M., Wang JY., Zhang Y., Zhang Y., Xiao L., Yu TX., Li JZ., Rao JN., Turner DJ., Gorospe M., Wang JY. jywang@som.umaryland.edu Accepted 2020-NOV-25 AT 08:05 GMT AT 08:05 GMT by ORCHARD Molecular and cellular biology 2017 imex curation mrna_igf2r_rat insulin-like growth factor 2 receptor mrna mrna messenger rna mRNA mrna rat Rattus norvegicus (Rat) Rat AGCTTCGACTCCGCTGTTGCGGCGCGATCGTGTCCCAGGCGCGGCTCGGAACGGCCAGCCGCCGTGAGCCCCACGCCACACGCGATGCGGGCCGTTCTGCCGGGACCCGTGCCCTCCGGGCCGCGCGTCGCGCTCCTGCTGCCGCTCCTGCTGCTGCTGCTCGTGGCGGCCGCGGGCTCCGCGCAGGCCCAGGCCGTGGACTTGGACGCCTTGTGCAGTTACACATGGGAAGCTGTTGATTCCAAAAATAATGCAGTTTATAAAATCAACCCCTGTGGACATGTGGATAATCCCAGGTGTGGGCCGACAAGTGCTGTCTGTATGTGTGACCTAAAGTCAGAAAACTGCCGGTCAGTGGGTGACTCACTTTTGAGAAGTTCAGCCAAATCACTCCTGGAATTTAACACCACTACGGGCTGCCAGCCTTCAGAGCACAGAATCCAGACTAGCATTACATTTCTGTGTGGGAAGACCCTGGGAACTCCTGAATTTGTAACTGCCACAGACTGTGTGCATTACTTCGAATGGAGGACTACTGCAGCCTGCAAGAAAGACATATTTAAAGCTGATAAGGAGGTGCCATGCTATGTATTTGATGACAAGTTACAGAAGCATGATTTGAACCCACTGATCAAGCTGAATGGCGGCTACCTGGTGGATGACTCTGACGCTGACGCATCTCTGTTCATCAATGTGTGTAGAGACATAGACTCCCTTCGGGACCCCAGCACACAGCTGAGAGTCTGCCCTGCTGGCACTGCTGCCTGTCTGCTGAAGGGGAACCAGGCATTTGATGTTGGCCGGCCCAAGGAGGGGCTGAAGCTATTGAGCAAGGACAGGCTCGTTCTGACTTATGTTAAGGAAGAAGGAGAAAAGCCAGACTTCTGTAATGGTCACAGCCCTGCAGTGACAGTAACATTTGTGTGCCCATCTGAGCGGAGAGAGGGCACCATTCCTAAACTCACTGCCAAGTCCAACTGTCGCTATGAAGTTGAGTGGATCACTGAGTATGCCTGCCACAGAGATTACTTGGAGAGTGAAACCTGCTCCCTCAGCAGTGAGCAGCATGATATTGCTATTGACCTTAGTCCCTTGGCCCAGCATGAAGAAGGGTCCCCCTATGTAGCTGATGGAGGAGAATATAGGTTTTTTATGAATGTCTGTGGGGACACAAAGGTCTCACTCTGTAACAAAGAGGCTGCTGTTTGTCAAGAGAAAAAGGTGGATTCCACTCAAGTCAAAATAGCAGGAAGACACCAGAACCAGACACTCCGGTACTCAGATGGAGATCTCACCCTAATCTATTCTGGAGGTGATGAGTGTAGTTCCGGCTTCCAGCGGATGAGTGTCATAAACTTTGAGTGCAACAAAACCGCAGGTCAAGATGGGAGAGGAGAGCCTGTGTTCACAGGTGAGGTGGACTGCACATACTTCTTCACGTGGGACACTAAATATGCCTGTGTAAAAGAGAAGGAAGATCTCCTCTGCGGGGCCATCGACGGCAAGAAACGTTACGACCTGTCTGTGCTGGCTCGTCACTCAGAATCAGAACAGAATTGGGAAGCAGTGGATGGCAGCCAGGCTGAGTCAGAAAAGAGGAATTTCTTCATCAATGTCTGTCACCGGGTGCTGCAGGCGGGAAAGGCCAAGAACTGTCCTGAAGGTGCTGCCGTGTGTGCTGTGGATAAGAGTGGAAGTAAAAATCTAGGAAAATTTGTCTCTTCTCCTACAAAAGAGAAAGGACACATTCAGCTCTCTTACTCTGATGGCGATGACTGTGGTAATGATAAGAAAATAACAACTAACATCACATTTGTGTGCAAACCAGGTGATCTGGAAAGTGCTCCAGTGCTGAGAGCTGCAGGGCCCGATGGCTGCTCCTATGAGTTTGAGTGGCACACAGCTGCTGCCTGTGTCCTGTCGCAGACGGAGGGAGAAAACTGCACTGTCCTTGATGCTCAGGCAGGGTTTTCTTTTGATTTGTCACTCCTCACAAAGAAGAATGGTGCATATAAAGTTGAGACAGACAAGTATGACTTCTACATAAATGTTTGTGGCCCTGTATCCGTGAACCTGTGTCAGTCAAACTCAGGAGCCTGCCAGGTAGCAAAAAGTGGTAAGTCTTGGAACTTGGGGCTGAGTAATACTAAGCTTACGTACTATGATGGGATGATCCAGCTGAGCTACAGGAATGGCACGCTGTATAACAATGAAAAGCACACACCGAGATCCACACTGATTACCTTCCTCTGTGACCGTGATGCTGGAGTGGGCTTCCCAGAATATCAGGAAGAGGACAACTCTACCTACAACTTCCGGTGGTACACCAGTTATGCTTGCCCGGAAGAGCCCCTGGAGTGCATGGTGACAGACCCCTCCATGATGGAGCAATATGACCTCTCCAGTCTAGTGAAGTTTGAAGGTGGCCGTGGAGGAAACTGGTATGCCATGGAAAACTCCCGAGAACATTTCACACGGAGGAAATACTACCTCAACGTGTGCCGGCCTCTGAATCCTGTGCCAGGCTGTGATCGATATGCATCTGCTTGCCAGATGAAATACGAAAACAATGAGGGCTCCTTGGCAGAGACTGTGGCCATCAGTAACTTGGGAGTTGCAAAGACAGGCCCTGTGGTGGAGGAGAGCGGCAGCCTCCTCTTAGAGTATGTGAACGGCTCTGCGTGCACCACCAGTGATGGCCGGCTAACCACCTACAGCACCAGGATCCATCTTGTGTGTGGTCGAGGAACTATGAATTCCCATCCCATATTCACTTTCAACTGGGAATGTGTGGTCAGTTTCTTGTGGAACACGGAGGCTGCCTGCCCCATCCAGACGATCACAGATTCAGACCAGGCTTGCTCTATAAGGGACCCCAACAGCGGATTTGTGTTTAATCTGAGCCCACTTAACTATTCTCAAGGACACATGGTCTTAGGCATTGGGAAGACTTTTGTGTTCAATATCTGTGGTACAATGCCTGCCTGTGGAACGGTTGCAGGGAAGCCAGCCCTTGGCTGTGAGGCAGAAACTAAGATCAAAGACATTAAGGATCTGAAGCCAGAAAGGCCAGTCGGGATGGAGAAGAGCCTCCAGTTGTCTGCCGAGGGGTTCCTCACTCTGACCTACAAAGGCTCTTCTCCTTCTGACAGAGGCACAGCCTTCATCATCCGTTTCATTTGCAATGGTGACATTTACCCAGGGACCCCCAAGTTCCTGCATCAGGACATTGACTCTGCACGAGGGATCCGGAACACCTTCTTTGAGTTTGAAACTGCATTGGCTTGTATTCCTTCTGTAGTGGATTGTCAAGTGACTGACCCAGCTGGGAACGAGTACGACCTGAGTGCCCTGAGCATGGTCAGGAAGCCGTGGACTGCTGTGGACACGTCTGTGCATGGGAAGAAGAGGCGCTTCTATCTGAGCGTGTGCACCCCACTGCCTTACATTCCTGGCTGCGATGGCATTGCAATGGGGTCCTGCATGGTGTCAGAAGACAAAAGTCAGAACTTGGGTGTGGTGCAGATCAGTCCTCAAGCTACTGGAAACGGATCTCTCAGCATCCTGTATGTGAATGGGGATAGGTGTGGAAACCAGCGCTACTCCACCAGGATAGTGTTCGAATGTGCCCAGACGTCGGGCTCACCTATGTTCCAGCTCCTGAACAACTGTGAGTATGTGTTTGTGTGGCGAACAGTGGAGGCCTGCCCTGTGGTCAGAGAGGAAGGAGACAACTGCCAGGTGAAAGACCCCAGACACGGCAATTTGTATGACCTGAAGCCCCTGGCCCTCAATGACACCATCATCAGTGCTGGCGAGTACACCTACTACTTCCGGGTCTGTGGAAAGCTGTCCTTAGATGTGTGCTCTGCCCATGATGGGTCCAAGGCAGTCTCATCATGCCAGGAAAAGAAAGGACCACAAGGATTCCAGAAAGTGGCAGGGCTCCTTAATCAGAAGCTGACTTTCGAAAATGGACTGTTGAAAATGAACTACAGTGGGGGGGACACCTGCCATAAGGTGTATCAGCGCTCCACAACCATCTATTTCTACTGTGACCGGACGACTCAGAAGCCAGTGTTTCTGAAGGAAACTTTGGACTGCTCCTATTTGTTTGAGTGGCGAACACAGTATGCCTGCCCACCTTTCAATGTGACAGAGTGCTCTATCCAGGATGAGGCTGGCAACTCCATTGACCTCTCCTCACTGTCAAGATACAGTGACAACTGGGAGGCTGTGACCAGGACAGGGGCCACTGAGCATTACCTCATCAACGTATGCAAGTCTCTGTCTCCACAGGCTGGCACAGATCCATGCCCTCCAGAGGCAGCTGTTTGTCTGCTGGATGGCTCCAAGCCTGTGAACCTCGGCAGGGTGAGGGATGGTCCTCAGTGGACAGCTGGTGTGACTGTCCTGAAGTATGTGGATGGTGACTTGTGCCCCGACAAAATTCGGAAAAGGTCAACCATTATTCGCTTCACCTGCAGTGACAGCCAAGTGAACTCCAGGCCTCTGTTCATCAGTGCTGTACAGGACTGTGAGTACACATTTTCCTGGCCCACACCTGCTGCCTGTCCTGTGAAGAGCAACATACACGATGACTGCCAAGTCACCAACCCCAGCACAGGCCACCTGTTTGACCTGAGCTCCCTAAGTGGCAAAGCTGGCATCACAGCCTCCTACAGCGAGAAGGGGATGGTCTTCATGAGCATCTGTGAGGAGAATGTGAACTGTAGCCCTGGAGTAGGGGCCTGCTTTGGACAGACCAGGATCAGTGTGGGCCAGGCCAGCAAGAGGCTGAGCTACAAGGATCAGGTCTTGCAGCTGGTGTATGAGAATGGGTCCCCATGCCCCTCCAAGTCAGGCCTGAGGTACAAGAGTGTCATCAGCTTCGTGTGCCGGCCGGAGGCTGGACCCACCAACAGGCCCATGCTCATCTCCTTGGACAAGCAGTCGTGTACACTCTTCTTCTCCTGGCACACGCCCCTGGCCTGTGAGCAGGCGACGGAATGCACCGTGCGGAATGGAAGCTCGATTATTGACCTGTCACCTCTCATCCACCGCACTGGTGGTTATGAAGCATACGATGAGAGTGAGGACGACACCTCTGATACCACCCCTGACTTCTACATCAACATCTGTCAGCCACTGAATCCCATGCATGGAGTGCCCTGCCCTGCAGGCGCCTCAGTATGCAAAGTCCCCGTGGACGGGCCCCCTATAGATATTGGGAGAGTCACAGGCCCTCCAATATTCAATCCCGTGGCCAATGAGGTCTACCTGAACTTTGAGAGCAGCACTCCTTGCTTAGCGGACAAATATATGAACTACACCTCACTAATTGCGTTTCACTGCAGGAGAGGGATCAGCATGGGAACTCCTAAGCTGATAAGGAACAATGACTGTGACTTTGTGTTTGAGTGGGAGACTCCGATTGTCTGTCCTGACGAAGTGAAGACACAGGGCTGTGCTGTAACAGATGAGCAGCTGCTCTATAGCTTCAACCTGACGAGCCTTTCCACAAGCACTTTCAAAGTCACTCGGGACGCTCACACATACAGTATAGGGGTGTGCACAACGGCAGCTGACTTAGACCAGGAAGGCTGCAAGGACGGCGGGGTCTGTCTGCTCTCTGGGAGCAAAGGAGCTTCTTTTGGGCGCCTAGCGTCGATGCAGCTGGATTACAGGCACCAGGATGAAGCAGTCATCCTGAGTTATGTGAACGGTGATCCTTGCCCTCCAGAAACGGAAGATGGCGAGCCCTGTGTCTTCCCCTTCATTTACAAAGGGGAGACCTATGACGAATGTGTATTAGAGGGACGAGCGAAACTGTGGTGTAGCAAAACTGCGAATTACGATCGTGACCACGAGTGGGGCTTCTGCAGACCGAGCAACAGTCACCGGATGTCTGCGATCATATTTACTTGTGATGAGAACGAAGATATTGGGAGGCCAGAAGTGTTCAGTGAAGATCGTGGGTGTGAGGTGACATTTGAGTGGAAAACAAAAGTTGTCTGCCCTCCGAAGAAGATGGAGTGCAAGTTTGTTCAGAAGCATAAAACCTATGACTTGCGATTGCTCTCATCCCTCACGGGGTCCTGGGATTTTGTGCATGAAGGAAACTCGTACTTTATCAATCTGTGCCAAAGAGTATATAAAGGTCCCCTGGACTGCTCTGAGAGAGCCAGCATTTGCAAGAAGAGTGCCACTGGTCAAGTCCAGGTTTTGGGGCTTGTTCATACTCAAAAACTGGAAGTCATAGATGAAACAGTCCTTGTCACTTACTCAAAAGGCCATTCTTGTGGTGGGAATAAGACTGCATCCTCAGTGATCGAGTTGACCTGTGCAAAGACTGTGGGCAGGCCTGCATTCAAGAGGTTTGATATTGACAGCTGCACCTACTACTTTTACTGGTACTCACGGGCTGCCTGTGCTGTGAGACCTCAGGAGGTGAACATGGTGAATGGAACTCTTACCAACCCTGTGACTGGCAAGAGCTTCAGCCTCGGGGAAATTTATTTTAAGCTGTTCAGCGCCTCTGGGGACATGAGAAGCAATGGGGACAACTATCTGTATGAGATCCAGCTCTCCTCTATCTCCAGCTCTTCTAACCCTGCGTGCTCCGGTGCCAACATCTGCCAGGTGAAGCCCAATGACCAGCACTTCAGCAGGAAAGTGGGCACCTCTGACATGACCAAGTACTACGTCCAAGACGGTGATCTGGATGTGGTGTTTACCTCTTCCTCCACGTGTGGCAAAGATAAGACCAAGTCTGTGTCTTCCACCATCTTCTTCCACTGTGACCCTCTGCTGAAGGACGGGGTCCCCGAGTTCAGCCACGAGACTGCTGACTGCCAGTACCTTTTCTCCTGGTACACTTCAGCTGTGTGTCCTCTGGGTGTGGACTTTGATGATGAGAATGCTGTGCCAGAGTATAAAGGGCTCTCAGAGCGGAGTCAGGCCGTTGGGGCAGTCCTCAGTCTCCTCCTGGTGGCACTGACTGGCTGTCTGCTGGCCTTGCTGCTTCATAAGAAGGAGAGAAGGGAAACTGTGATAAACAAGCTGACCAATTGCTGCAGAAGGAGCTCGGGGGTGTCCTATAAGTACTCAAAGGTCAGCAAGGAGGAGGAGACAGATGAGAATGAGACAGAATGGCTGATGGAAGAGATCCAGGTGCCAGCCGCCAGACTAGGCAAGGACGGGCAGGAGAACGGCCACATTACCACCAAGACGGTGAAAGCAGAAGCTCTCACTTCCCTGCATGGGGATGACCAAGACAGTGAGGATGAGGTCCTGACCATCCCAGAGGTGAAAGTTCACACAGGCAGAGGAGCCGAGGTGGAAAGCTCACAGCCCCTGAGAAATCCCCAGCGCAAGGTCCTGAAGGAAAGGGAGGGGGAGAGGATGGGGCTGGTCCGCGGTGAGAAGGCCAGAAGAGGGAAGTTCAGACCCGGACAGCGCAAGCCAACAACCCCTGCCAAGCTGGTGTCCTTCCATGATGACAGTGACGAGGACCTCCTACACATCTGACTCCGTGGTGCCTACAGGAGAAGGTGGCACCATGGACAGCCAAGCATCTCCAACCAAATAAGACTTCAGCTCGATGATGCGTCTGTCATTTTGCCTTTAACAAAACTGCTTCAAAGGGAAGTTTCTGTAATGGGGGAGAGGATGGGGGAGGTGGGCACCACTCTTCCATGATCGTTTACAGTCAGTGGAACAAGGTGTTGCTTGAATCGGCCAAAGGGCGCTACTTGGTGCCCAGGGTTTTGCCCCAAGTCCTCACTTACGATCATAGCTGCTGTGTGCTTCCAACAGGTGCCCAGGCCACATGCATCTCACAACAGAGGGCTGTGTTTGAAAAAAACCCTTGCTGCTTTAGACCCTATAGGGTGACCATTTATATTTTTAGCATTGTAATTCTCTCCCCCTATTTATTGACTTTGACAATTACTACTCAGGTTTGCGAAAAAGGAAAAAAAAAAAAAACCAGTTTCTTCTGCCAGCAAGGGCATAAGGTGAAGCTGGCATTATGCTGGAGCCTTGTACGTGGCTCCTGAGCTGGTCCTTGATGCACACTTTAGTGAGCTGCAGTATTCTATTCTTCGCTTTGCGTTTTGTAAGGGCACACATACATGCACAGGTCCCCGAGTGCCTCGGTTTGGGAAGGCCCGCCTGCTCCGTGCCCTACCTCGTCTCTATAGCTGCAGCTCTTTGCACGAATCCTTCTGGGTGTCGCTTTTGCCCGCTGGTGAGCGCCAGCAGCTCACCCAGAGGTGGGTGTTTGTGCATGCGATGGAGTAGAGGCTCTTGGAGAGGAGCCGCACTTCAGGTTTGGGCTGTAGGTCTCATCTCTTCAGGTTCTCAATGCCACCTTTACTGTGCTTTTTGCTTTTATTTTAAGGGGGAAATGTTTATCACCCATTCAACCTATAAGAAGCCTTAATTTGCACAGGGTGACTTACAGACACCGTGAAATACGGTGGGCCAGTGTTGTGTGTGGGCTTGTGCAGGGCAGTGTCCCGGGATGGAAGGCCAGGCCCGGTCCACTGGCTGTGGACTCCTTTCCTGATGTGTTCAATGACTTCTCACATTATAGAGTTGTATATAGGTGGTATTTACATACCCAGAAGCAAAGTACCCCGCAGAATTTCTTGGTTTATGCCTGATTTTGCTAGCTTATTTGATTTCACATTGAGTGTATTTTTAAAAATTGATTTTTTTCTTCATTTTTTAATCAACTTTACTGTAATATGAAGTATTCAATTTCAATAAAAATAAATTATT mrna_stim1 stromal interaction molecule 1 mrna messenger rna mRNA mrna rat Rattus norvegicus (Rat) Rat TGGCCCGTGCGCGTCCGCCTGCTGCACCGGGGGACCAGGAGCCGCAGAGGTACGGGACCCGGCGGGGGCGCTGACCTCGGCCTAGGAGTCTTAGGATCCTAGAGAATCGGTGTCTGCTAGGGCCTGTAGGTCGCACGCCGAAGCCCTAGCTGCTGAGGCTCACCGCTGGTCCGCGGGGCGAGGTCAGGTGCCCCCCTTCTCTCCTCCCTTCTCTCCCTCCCCCACCTCCGTGCGGGCGGGAGATTCTGCCCGCCTCCTCCCGCAGGGGTGCAGCAAGCTGCGGAGCTGACAGCGGCCCCGCAGCCACCCTGCTCAAACTCTCCGGAAGCAGCTAGAGCTCAGGCCGCCGCCGCCGCAGCTTCGGCGGACCCACAGTTGGACCGGAGACTCCGATCCTTCTGCGTCCAAACTTGGGGCACTTGACCTTCGGTTGTCGAAGGAGGTGTCTACAGCAGGCGCATGTTGACCGAGACCTTCCGTCATGGATGTGTGCGCTCGTCTTGCCCTGTGGCTTCTTTGGGGGCTCCTTCTGCATCAGGGCCAGAGTCTCAGCCATAGTCACAGTGAGAAGAATACAGGAGCTAGCTCGGGGGCGACTTCTGAAGAGTCTACCGAAGCAGAGTTTTGCCGGATTGACAAGCCCCTGTGCCACAGTGAAGATGAGAAGCTCAGCTTTGAGGCCGTCCGCAACATCCACAAGCTGATGGATGATGATGCCAATGGTGATGTGGATGTGGAAGAAAGTGATGAGTTCCTAAGGGAAGACCTCAATTACCATGACCCTACAGTGAAACACAGCACCTTCCATGGTGAGGACAAGCTTATCAGCGTGGAGGACCTATGGAAGGCATGGAAGGCATCAGAAGTGTATAACTGGACCGTGGATGAGGTGATACAGTGGCTGATTACGTATGTGGAGCTGCCACAGTATGAGGAGACTTTCCGGAAGTTGCAGCTTACTGGCCATGCCATGCCAAGGCTAGCTGTAACAAACACCACCATGACAGGGACTGTCCTGAAGATGACAGACCGGAGCCACAGGCAGAAGCTGCAGCTGAAGGCTCTGGACACAGTGCTGTTCGGGCCTCCTCTCTTGACTCGCCACAATCACCTGAAGGACTTCATGCTGGTGGTATCTATCGTGATTGGTGTGGGTGGCTGCTGGTTTGCCTATATCCAGAACCGTTACTCTAAGGAGCACATGAAGAAAATGATGAAGGATCTGGAAGGATTGCACCGAGCTGAGCAGAGTCTGCATGACCTTCAGGAAAGGCTGCACAAGGCCCAGGAGGAGCACCGAACAGTGGAAGTAGAGAAGGTCCACCTGGAGAAGAAGCTGCGTGACGAGATCAATCTTGCCAAGCAGGAAGCTCAGCGGCTGAAGGAGCTGAGGGAGGGTACCGAGAATGAGAGGAGCCGCCAAAAATATGCTGAGGAAGAGCTGGAGCAGGTTCGGGAGGCTTTGAGGAAAGCAGAGAAGGAGCTGGAATCACACAGCTCCTGGTATGCTCCTGAGGCCCTGCAGAAGTGGCTGCAGCTGACACATGAGGTGGAGGTGCAGTACTACAACATCAAGAAGCAAAATGCAGAGAGGCAGCTGCTGGTGGCCAAGGAGGGGGCTGAGAAAATAAAAAAGAAGAGAAACACGCTTTTTGGTACCTTCCATGTGGCCCACAGCTCGTCCCTGGATGACGTGGATCATAAAATCTTAACTGCTAAGCAGGCACTGAGTGAGGTGACAGCAGCCCTGAGGGAGCGCCTGCACCGGTGGCAGCAGATCGAGATCCTCTGTGGTTTCCAGATTGTCAATAACCCTGGCATCCACTCCTTGGTGGCCGCGCTCAACATAGATCCCAGCTGGATGGGCAGTACCCGCCCTAACCCTGCCCACTTCATCATGACTGACGATGTGGATGACATGGATGAGGAGATCGTGTCGCCCTTGTCCATGCAGTATGCTGCCTGGCTGATGGGGCGTAGGTTCAGTGACCGCTCTCTCTGCTCTGCATCCGCCGGCTCGGATGATCAGTCCCTCTGGAAATACCCGGCCCCCAGCCTGCAGAGCAGTGTCCGGCAGCGCCTGACGGAGCCACAGCATGGCCTGGGATCTCAGAGGGATTTGACCCATTCCGATTCGGAGTCCTCCCTCCACACGAGTGACCGCCAGCGTGTGGCCCCCAAGCCTCCTCAGATGGGCCGTGCTGCAGATGAGGCTCTCAATGCCACGTCTTCCAATGGTAGCCATCGGCTGATTGAGGGGGTCCATCCAGGATCTCTGGTGGAGAAACTGCCTGACAGCCCTGCTCTGGCCAAGAAGACAATCCTGGCGCTGAACCATGGCCTAGATAAGGCCCACAGCCTGATGGAGCTGAACCCCTCAGTCCCACCTGGTGGCTCCCCACTTTTGGATTCTTCCCATTCTCATAGCCCCAGCTCCCCAGACCCAGACACACCATCTCCAGTTGGGGACAGCCGAGCCCTGCAGGGTAGCCGAAACACACGAATTCCCCACTTGGCTGGCAAGAAGGCTATGGCTGAGGAGGATAATGGCTCTATTGGTGAGGAGACAGACTCTAGTCCAGGCAGGAAGAAGTTTCCCCTCAAAATTTTTAAGAAGCCTCTTAAGAAGTAGGCAGAATAGGGTGGTAGTATTGACACAGCCTGTCCTTCCCTGGGGCTTCTGCCTTCACTTCCCTTCCTTTTTTTGAGATATCTGGCCCCTAGAGTGGGCCATGGAGGGGCTGGCCCAAGGGCCTGGGCACTGTACATACCTGCCCTGCTCATCCTTGGTCCTTCATCATTATTTATTAACTGACCGCCATGGCCTGCCTGCCCTGAAATCCTTCCAACCACGGGCTGCTGCTGTCACATCCTCTCCACTTCAGTGCATGTCTTAGTTGCTGTTCCCTCAATCCCACTCCACTTTTGGGGTCCAGCTTCTGTCTCTGCTGTCCCAGTTTAGAGGTTTGGTTTTTGTTTCTGTCTCCTGCTTTCAGGCTCCTCTCTCCCATTACTCCCCCATGTTCCTAGCAGTTGTGGGGAAGATAGGAGGAGTAACTTCTGACACCTGTACCTCAGATCTATTCATACTGCTCACAATCATTCTGCTTACCCCAGACTGGGCCAAGGTCCTAATCTCTGGGGATTGTTCTATGGAAGGTGGTGGACCGGAGGGAACCTCACCCTGGAGCTCATTTGGATCTCCAGGGCTCCATTCAGACAGTGGAACCAACTCCCAGGGAACAGATCAGCAGAGTTTCGAAGAGAGACCAGGCTTGTGACTGTGGGAAAGACTTCTCTGAGTTGGAGGATGAGTAGATGCCAAAGCTGTGGGCTCCGAGGCAGTTGCCATTTCCCTCTTGCCCTGCCCACATCTGCCTTCCTCTTACCTCTGCTCCCCTATATTGTAGGAGTGGGTCTCTTAAGAGGTGCTGCCCTGAAGCTCCTCAAGCATCAGTACTCCTGGGGCTCAGGACAACTGGCTCCCCTGGCTATGGGAGCCACAGTCATGACACGGGGCTCTTGTGGAGCCCTAGACAAGGATGTTGTATCTGAACCAAAAGACAAACGGTTTTAAAGTAAAAAAATAAAAACAAAAGAAATAAATCTCCTGAATTTCCCAAGTGCCTGCACAGCATCCTCCCCTTGTCAGACTCCATTTTCGTGTACTGCATAGCCTGGGCCACAGGCTCCAAACAGACACAGTTGGTTTGGGGAAGGGGTGGAGAAGGAAGATGGTATATGTGCGTGAAGCTGCTGTGTGACCACTTCTCCCCCCTGACGCCTCCCCTCTTCCAGACAACTCTCTCCTTTACCTGGTTTTGCTATGGCTGTAAAGATATTTTCCCTCTGCCCCATTCTGTCATGCCTTTACCCTGAGTTCCTAATTTGGGCCTGGGTTGGGTGCAGCTGGGGCTGGTCTTAGGTGGGTGCTAGGCTCTAGACTGCCTCGGTGCCCCCTGGACACCCTCACATGGGTTTTTCTGTGTTATTTTCATAAGATTCTTTGAAGTCCAATAAAGCATGTAGGAGATTTTAACCTCTG rno-mir-195-5p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA rat Rattus norvegicus (Rat) Rat UAGCAGCACAGAAAUAUUGGC mrna_cdk4 cyclin-dependent kinase 4 mrna messenger rna mRNA mrna rat Rattus norvegicus (Rat) Rat ATGGCTACCACTCGATATGAACCCGTGGCTGAAATTGGTGTCGGTGCCTATGGGACGGTGTACAAAGCCCGAGATCCCCACAGTGGCCACTTTGTGGCTCTCAAGAGTGTGAGAGTTCCTAATGGAGGAGCAGCTGGAGGGGGCCTTCCCGTCAGCACAGTTCGTGAGGTGGCCTTGTTAAGAAGGCTGGAGGCCTTTGAACATCCCAATGTTGTACGGCTGATGGATGTCTGTGCTACTTCCCGAACTGATCGGGACATCAAGGTCACCTTAGTGTTTGAGCATATAGACCAGGACCTACGGACATACCTGGACAAAGCACCTCCGCCGGGCTTGCCTGTTGAGACCATTAAGGATCTGATGCGCCAGTTTCTAAGCGGCCTAGATTTCCTTCATGCAAACTGCATTGTTCACCGGGACCTGAAGCCAGAGAACATTCTAGTGACAAGTAATGGGACAGTTAAGCTGGCCGACTTTGGCCTAGCCAGAATCTACAGCTACCAGATGGCCCTCACGCCTGTGGTTGTTACGCTCTGGTACCGGGCTCCTGAAGTTCTTCTGCAGTCTACATATGCAACGCCTGTGGATATGTGGAGTGTTGGCTGTATCTTCGCAGAGATGTTTCGCCGGAAGCCTCTCTTCTGTGGGAACTCTGAGGCTGACCAGCTGGGCAAAATCTTTGATCTCATTGGATTGCCTCCAGAAGACGACTGGCCTCGAGAGGTCTCTCTTCCTCGAGGAGCCTTTTCCCCCAGAGGACCTCGGCCAGTGCAGTCAGTGGTGCCGGAGATGGAGGAATCTGGAGCGCAGTTGCTGCTGGAAATGCTGACCTTTAATCCACTTAAGCGAATCTCTGCCTTCCGAGCCCTGCAGCACTCTTACCTGCACAAGGAGGAAAGTGACCCGGAGTGA celf1_rat CUGBP Elav-like family member 1 Celf1 Cugbp1 Bruno-like protein 2 CUG triplet repeat RNA-binding protein 1 CUG-BP- and ETR-3-like factor 1 RNA-binding protein BRUNOL-2 protein protein rat Rattus norvegicus (Rat) Rat MNGTLDHPDQPDLDAIKMFVGQVPRTWSEKDLRELFEQYGAVYEINILRDRSQNPPQSKGCCFVTFYTRKAALEAQNALHNMKVLPGMHHPIQMKPADSEKNNAVEDRKLFIGMISKKCTENDIRVMFSSFGQIEECRILRGPDGLSRGCAFVTFTTRTMAQTAIKAMHQAQTMEGCSSPMVVKFADTQKDKEQKRMAQQLQQQMQQISAASVWGNLAGLNTLGPQYLALYLQLLQQTANSGNLNTLSSLHPMGGLNAMQLQNLAALAAAASAAQNTPSGTNALTTSSSPLSVLTSSAGSSPSSSSSNSVNPIASLGALQTLAGATAGLNVGSLAGMAALNGGLGSSGLSNGTGSTMEALTQAYSGIQQYAAAALPTLYNQNLLTQQSIGAAGSQKEGPEGANLFIYHLPQEFGDQDLLQMFMPFGNVVSAKVFIDKQTNLSKCFGFVSYDNPVSAQAAIQSMNGFQIGMKRLKVQLKRSKNDSKPY D39AD132660EBF40 mrna_igf2r_rat-rno_mir_195_5p-1 2 5 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed engineered engineered region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain tag luciferase tag luciferase tag 3' range 3' range 3' range 3' range 7 predetermined predetermined participant predetermined tag luciferase tag visualisation by luciferase assay tag luciferase regulator regulator modulator regulator neutral component neutral component cell lysate cell lysate engineered engineered physical association physical association Fig 3 A D E down-regulates quantity by destabilization rno_mir_195_5p-mrna_igf2r_rat-2 1 6 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level 5 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level 7 predetermined predetermined participant predetermined qrt-pcr quantitative reverse transcription pcr QRT-PCR unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate engineered engineered tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig 3 C celf1-mrna_cdk4-1 3 8 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 5 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 9 antibody detection identification by antibody antibody detection rt-pcr reverse transcription pcr RT-PCR unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level association association Fig 4 A mrna_igf2r_rat-celf1-1 4 5 predetermined predetermined participant predetermined western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate engineered engineered region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 9 antibody detection identification by antibody antibody detection western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level association association Fig 4 B C, Suppl Tab 1