DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Cryo-EM structure of the spinach chloroplast ribosome reveals the location of plastid-specific ribosomal proteins and extensions. Cryo-EM structure of the spinach chloroplast ribosome reveals the location of plastid-specific ribosomal proteins and extensions. Nucleic acids research 2017 imex curation Graf M., Arenz S., Huter P., Dönhöfer A., Novácek J., Wilson DN. wilson@lmb.uni-muenchen.de 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation spiol Spinacia oleracea (Spinach) Spinach 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Graf M., Arenz S., Huter P., Dönhöfer A., Novácek J., Wilson DN. wilson@lmb.uni-muenchen.de Accepted 2021-JAN-15 AT 04:42 GMT AT 04:42 GMT by KALPANAP Nucleic acids research 2017 Only protein-protein interactions imex curation imex curation rk20_spiol 50S ribosomal protein L20, chloroplastic Chloroplastic large ribosomal subunit protein bL20c rpl20 protein protein spiol Spinacia oleracea (Spinach) Spinach MTRVKRGYIARRRRKKIRFFASSFRGAHSRLTRTIAQQKIRALVSAHRDRDRQKRDFRRLWITRINAAIRERGVYYNYSKFIHDLYKRQLLLNRKILAQIAILNPNCIYMIYNEIIKKEDCKKYLEII 79926508C9A03EEB rk18_spiol 50S ribosomal protein L18, chloroplastic CL18 Chloroplastic large ribosomal subunit protein uL18c RPL18 PRPL18 SOVF_159210 protein protein spiol Spinacia oleracea (Spinach) Spinach MAAATSLSFFHSTLASSSSSSVQQLSLPPKFVNFRPQTLPLIQAKAHTRREDRTARHVRIRKKVEGTPERPRLCVFRSNKHLYVQVIDDTKMHTLAAASTMQKAISENIDYSAGPTVEVAQKIGEMIAKSCLEKGITKVAFDRGGYPYHGRVKALADAAREHGLVF 98EA5F95A30040E5 rk24_spiol 50S ribosomal protein L24, chloroplastic CL24 Chloroplastic large ribosomal subunit protein uL24c SOVF_183870 RPL24 protein protein spiol Spinacia oleracea (Spinach) Spinach MAAMAALQSSFTSLSLSSNSFLGQRLFPSPTTLQVKTEGHSPCLIVMRIKRWERKDCKPNSLPKLHKMHVKVGDTVKVISGGEKGKIGEISKIHKHNSTVIIKDLNLKTKHVKSKEEGEQGQIIKIEAAIHSSNVMLYSKEQEVASRVGHKILEDGRKVRYLIKTGEIVDTPDRWKEIQNKKESETAVAVAA 7615B885AFADF1FE rk35_spiol 50S ribosomal protein L35, chloroplastic CL35 Chloroplastic large ribosomal subunit protein bL35c SOVF_080710 RPL35 protein protein spiol Spinacia oleracea (Spinach) Spinach MAMASATATLSFKTPSLSLSPPSTRCSAAQGISLTHFNKQLKSTLNLSSSSSISSSKVQPIVLKNKRISTVDSSVSTSSPSFTVFAAKGYKMKTHKASAKRFRVTGKGKIVRRRAGKQHLLAKKNTKRKNRLSKLIQVDRSDYDNVIGALPYLKVNRKV C247FE36470007D1 psrp5_spiol 50S ribosomal protein 5 alpha, chloroplastic 50S ribosomal protein L40 CL40 Chloroplastic large ribosomal subunit protein cL37 Plastid-specific 50S ribosomal protein 5 alpha PSRP5 RPL40 SOVF_000530 protein protein spiol Spinacia oleracea (Spinach) Spinach MALLSPLLSLSSVPPITSIAVSSSSFPIKLQNVSVALLPSFGQRLVAHGPVIAQKRGTVVAMVSAAAEETAGEDGDQSKVEEANISVQNLPLESKLQLKLEQKIKMKMAKKIRLRRNRLMRKRKLRKRGAWPPSKMKKLKNV 87425931C3E4F93F rk6_spiol 50S ribosomal protein L6, chloroplastic Chloroplastic large ribosomal subunit protein uL6c SOVF_107200 RPL6 protein protein spiol Spinacia oleracea (Spinach) Spinach MSLPLPSHMKSVFLGMKVEISTSVPVTRIGFWRKSVDCKESRIGKQPITVPANVAIAMEGQDLKVKGPLGELSITYPREVLVEKQESGFLRVRKAVETRRANQMHGLFRTLTDNMVVGVSKGFEKKLQLVGVGYRATVEGKDLILSLGFSHPVRMAIPDELQVKVEENTKVTVSGRDKSVVGQFAATIRSWRPPEPYKGKGVRYVDEVVRRKEGKAGKKK 2E0BB3403003424E rk5_spiol 50S ribosomal protein L5, chloroplastic Chloroplastic large ribosomal subunit protein uL5c RPL5 PrpL5 SOVF_057730 protein protein spiol Spinacia oleracea (Spinach) Spinach MASTSLLQSTSSSFAGVRFHCRTSAAPRVGLSSFTVKAAAGTAVFVDKAEAETINRLKTNYIEKMVPLLKEEFSYSNILEVPKVVKIVVNCGIGDASQNAKGLDAAINELALITGQRPVKTKAKTSIAGFKVREGMTLGIAVTLRGNLMYSFLDRLINLALPRTRDFQGVNPNSFDGHGNYSVGFREQSVFPEIKPEIVGKARGMDVCITTTAKTDKEAYKLLSLMGMPFREGSGPSTLVRKKKLKSHHFDAKKGRRY 52C18B2D224AFF4C rk32_spiol 50S ribosomal protein L32, chloroplastic Chloroplastic large ribosomal subunit protein bL32c rpl32 protein protein spiol Spinacia oleracea (Spinach) Spinach MAVPKKRTSIYKKRIRKNIWKKKGYWAALKAFSLAKSLSTGNSKSFFVRKISNQTLE E1F7681D409B49D5 rk2_spiol 50S ribosomal protein L2, chloroplastic Chloroplastic large ribosomal subunit protein uL2c Ribosomal protein CS-L4 rpl2-A rpl2-B protein protein spiol Spinacia oleracea (Spinach) Spinach MAIHLYKTSTSSTRNGAVQVKSNPRNNLISGQRRCGKGRNARGIITARHRGGGHKRLYRKIDFRRNEKDIYGKIVTIEYDPNRNAYICLIHYGDGEKRYILHPRGAIIGDTIVSGTEVPIKMGNALPLTDMPLGTAIHNIEITLGRGGQLARAAGAVAKLIAKEGKSATLKLPSGEVRLISKNCSATVGQVGNVGVNQKRLGRAGSKRWLGKRPVVRGVVMNPVDHPHGGGEGRAPIGRKSPTTPWGYPALGRRSRKRNKYSDNFIIRRRSK 30D99EF32A743B75 rk3_spiol 50S ribosomal protein L3, chloroplastic Chloroplastic large ribosomal subunit protein uL3c SOVF_195350 RPL3 protein protein spiol Spinacia oleracea (Spinach) Spinach MAAILPTFSIKSSSCTYSSSKRSLSSPKAVSLSSSVNGTPKVQSLRLSTSFVCSPNQIIKLKSVSPSRSTQLRRAVGGLEIKMMSVDAGIGVMGTKLGMMSFFEEDGTVVPVTVIGFKEGNIVTQVKTESTDGYNAVQVGYERLRDRKLTMPERGHLNKAGVIPMRHLQEFRLVSVDDFTPSQKLLFEELFKEGDMVDISGTTIGKGFQGGIKRHNFKRGLMTHGSKSHRALGSIGAGTTPGHVYKGKKMPGRMGGTKTKIRKLKIMKIDTDLRVVMIKGAVPGKPGNLLRLAPAKIVGKNIPKN 6A4D1456370031F6 rk33_spiol 50S ribosomal protein L33, chloroplastic Chloroplastic large ribosomal subunit protein bL33c rpl33 protein protein spiol Spinacia oleracea (Spinach) Spinach MAKGKDVRVKVILECTGCVRKSVNKGSRGVSRYITQKNRHNTPSRLELRKFCPYCYKHTIHGEIKK 7AF55DECF5CCB291 rk34_spiol 50S ribosomal protein L34, chloroplastic CL34 Chloroplastic large ribosomal subunit protein bL34c SOVF_073030 RPL34 protein protein spiol Spinacia oleracea (Spinach) Spinach MATLSLLSTGVGAAITNRTPSASLTFITGSRTTNKRVSFNGGSARSGSLHCSFLAPSSSLSSNFSGLSLGLDLTSNTGVSTDRCRRFVVRAGKAALCLTKRSRSRKSLARTHGFRLRMSTTSGRALLKRRRAKGRKILCTKTNPSSGKRASP 755A990D441ADB18 rk28_spiol 50S ribosomal protein L28, chloroplastic CL28 Chloroplastic large ribosomal subunit protein bL28c SOVF_085320 RPL28 protein protein spiol Spinacia oleracea (Spinach) Spinach MAASGMLISNPSNVCFRKPQFSCSLKPKATVSELGFLTSQLSGIQISPSPLFPIISKPISAPLKPSLQPVARRICPFTGKKSNKANRVSHSNHKTKRLQFVNLQYKRVWWEAGKRFVKLRLSTKALKTIEKNGLDAVAKKAGIDLRKE 72C51FD8708A9761 rk19_spiol 50S ribosomal protein L19, chloroplastic CL19 Chloroplastic large ribosomal subunit protein bL19c RPL19 PRPL19 SOVF_150800 protein protein spiol Spinacia oleracea (Spinach) Spinach MASKVLPQALLVIPSNHSLQCPPLKKQLGFPIDSNRRFSLSSNCRSNLMVSRASSNLFSSNFSSIFSFPARNSFVVRSEAEDSSDAPAESVAVVAEEELPVESEAEAEERPPRQQRVKLGDIMGILNKKAVHAAEELRPVPGIRTGDIVQIRLEVPENKRRLSVYKGIVISRQNAGIHTTIRIRRIIAGVGVEIVFPLYSPNIKEIKVVSHRKVRKARLYYLRDKLPRLSTFK 62CC200F4019E392 rk17_spiol 50S ribosomal protein L17, chloroplastic CL17 Chloroplastic large ribosomal subunit protein bL17c SOVF_059230 RPL17 protein protein spiol Spinacia oleracea (Spinach) Spinach MIDNGGRFFAMKHGRKIHRLSRPADQRRALLRGLTTQLLKHGRIKTTRAKASAMRKYVDKMITLAKEGSLHKRRQALGFIYEKQIVHALFAEVPERYGDRNGGYTRIIRTLPRRGDNAPMAYIELV 4E31F663C2706EEC rk16_spiol 50S ribosomal protein L16, chloroplastic Chloroplastic large ribosomal subunit protein uL16c Ribosomal protein CS-L24 rpl16 protein protein spiol Spinacia oleracea (Spinach) Spinach MLSPKRTRFRKQHRGRMKGISYRGNRICFGRYALQALEPAWITSRQIEAGRRAMTRNARRGGKIWVRIFPDKPVTVRPAETRMGSGKGSPEYWVAVVKPGRILYEISGVAENIARRAVAIAASKMPIRTQFIISG 908A446567467290 rk4_spiol 50S ribosomal protein L4, chloroplastic Chloroplastic large ribosomal subunit protein uL4c SOVF_029280 RPL4 protein protein spiol Spinacia oleracea (Spinach) Spinach MATSTSSSLSLSFFSSSLFSSKSRNFSSKPILKLPSSSHSQTSLSLSIKSELIPLPILNFSGEKVGETFLNLKTAPPEKARAVVHRGLITHLQNKRRGTASTLTRAEVRGGGRKPYPQKKTGRARRGSQGSPLRPGGGVIFGPKPRDWTIKMNKKERRLALSTAIASAVGNSFVVEEFAENFEKPKTKDFIAAMQRWGLDPAEKSLFFLMDLVENVEKSGRNIRTLKLLTPRSLNLFDVLNAEKLVFTEGTIQYLNQRYGVDTLEDEDEEEEEEEEGEEVDDGVEDGTPEPAE 875C425F090826E5 rk23_spiol 50S ribosomal protein L23, chloroplastic Chloroplastic large ribosomal subunit protein uL23c PRPL23 SOVF_109700 RPL23 protein protein spiol Spinacia oleracea (Spinach) Spinach MATTAPNLHSLSSSFAFSNPSSNVSATSFTFQIPNKKAQISCISSKKLHTQKSFNFHDAVTPMNKPSFGRDLMVAQATEAVAPTTEEAATSQPKTSKKAKKLKYPRRILDVYQILQSPIITEAAIKNIADENSLLFTVDVRADKKMIREAISNFFGVKVRKVNTLIRPDGTKKAYIMLNKEYNASELAKKIGIFPGGN 624DF1D4032BB70F rk15_spiol 50S ribosomal protein L15, chloroplastic CL15 Chloroplastic large ribosomal subunit protein uL15c SOVF_177460 RPL15 protein protein spiol Spinacia oleracea (Spinach) Spinach MASLLSLSSTPPSTANSNNYPSSTFKGNINNFRINPFNFAPLKLHLRNIVKKESTRLVVVASASSSNVSPSIGSGSETRFRLDNLGPQPGSRKKGKRKGRGHAAGQGGSCGFGMRGQKSRSGPGIMRGFEGGQMPLYRRIPKLRGIAGGMRAGLPKYVPINLRDIEVAGFKEGEEVSLESLKAKGIINPSGRERRLPLKILGEGELSTKLQIKARAFSGSAKEKLEAAGCSVTVLPGRKKYIKESVRKNLARADEYFAKKRAASASEAESA 2310AEE56EE592D8 rk27_spiol 50S ribosomal protein L27, chloroplastic CL27 Chloroplastic large ribosomal subunit protein bL27c SOVF_107450 RPL27 protein protein spiol Spinacia oleracea (Spinach) Spinach MAVTTSMSFNLMASFRGMSLSSSSSSSFFKGEFGPSSLRLPNKSPLSVSPFPLTIESAHKKGAGSTKNGRDSKGQRLGVKIYGDQVAKPGAIIIRQRGTKFHPGKNVGIGKDHTIFALIDGLVKFEKYGPDKKKVSVYPREIQPENPNSYRARKRENFRLQREKKKARREGYSFQPQLILASAATDNADESAVC 7BB90273E8A5A944 rrfc_spiol Ribosome-recycling factor, chloroplastic CpFrr RRFHCP Ribosome-releasing factor, chloroplastic RRF FRRHCP protein protein spiol Spinacia oleracea (Spinach) Spinach MAASSLSSATSYLHSFRRRNSCVSLQGVSDMECNIARTNVSVWRSSANYVRMDCGVKKFSGKAVVVKQLQNRAGTFRCATMEEVEAEKSLIETNTKQRMEKTIETIRSNFNSVRTNRASPTMLDRIEVEYYGTPVSLKSIAQISTPDASSLLISPYDKSSLKAIEKAIVTSQLGVSPNNDGEVIRLSLPPLTSDRRKELAKVVSKLAEEGKVAVRNIRRDALKSYEKLEKEKKLSEDNVKDLSADLQKLTDEYMKKVESIYKQKEQELMKV F7D3012BB90AD175 rk29_spiol 50S ribosomal protein L29, chloroplastic CL29 Chloroplastic large ribosomal subunit protein uL29c SOVF_096810 RPL29 protein protein spiol Spinacia oleracea (Spinach) Spinach MLAIHSLSSTPCSSGLTSPPKSTLLTKSSFHGLRLPSVNLSSSLRLRVQTPPSSVVVMVKKEDELKELRTKTNEQLNEEILQLKGELFMLRLQRSARENFKPSDFGRMRKRVARMLTVKREREIEQGVGKRLSRKLDKAWKRSIVVRPPPSLKKLQEKATAEAEAEKA 176C2ECCC5943207 rk21_spiol 50S ribosomal protein L21, chloroplastic CL21 CS-L7 Chloroplastic large ribosomal subunit protein bL21c SOVF_056040 RPL21 protein protein spiol Spinacia oleracea (Spinach) Spinach MASATLAFSCSSLCATLKLPQNLNPLLLNVPPLSKPFSGVVSPPSLSRLSLLPVAAKRRRFQEIPEELKAEFEEFQRPPNQKPQLSDVLPDDFQAPEPGTPEYNDIINQFLPKKGPPPPREEIFAVVVIGSRQYIVIPGRWIYTQRLKGATVNDKIVLNKVLLVGTKASTYIGTPIVTNAAVHAVVEEQLLDDKVIVFKYKKKKNYRRNIGHRQPITRIKITGITGYEDYPASTLEAEVEAKEEAEAEAEAEAVPV 3FD2BE241C42BC79 rk13_spiol 50S ribosomal protein L13, chloroplastic CL13 Chloroplastic large ribosomal subunit protein uL13c SOVF_093170 RPL13 protein protein spiol Spinacia oleracea (Spinach) Spinach MATMACASSLTFPSAQTQKSFFGTNVKQTPVLSFPRPTVAAAVAVSARKSTSASTKCTEEWRQLKEAVKKEFAIPHVPLDQRWMFTLEEATGPDIWNTTWYPKSADHVPTDKKWYVVDATDLILGRMASTIAIHIRGKNLASYTPSVDMGAFVIVVNADKVAVSGKKRTQKLYRRHSGRPGGLKEETFDQLQKRIPERIIEHAVRGMLPKGRLGRYLFNHLKVYKGAEHPHQAQQPIDLPLRDKRIRVEK 0B8F157B7A948EA7 psrp6_spiol 50S ribosomal protein 6, chloroplastic CL25 Chloroplastic large ribosomal subunit protein cL38 Plastid-specific 50S ribosomal protein 6 SOVF_082300 PSRP6 protein protein spiol Spinacia oleracea (Spinach) Spinach MSVSAIFGARVVTIPSVLRTSSVDGRTVKLQPSTGGSCGGGVITIECSSRPQKKGTAHHMKTRPKKTARWDIKRGPAVYPPLPPLPAEWTIVSSAVDEADSSSSTTSSSAEIAQSA 02EF117AFEFC9340 rk36_spiol 50S ribosomal protein L36, chloroplastic Chloroplastic large ribosomal subunit protein bL36c rpl36 protein protein spiol Spinacia oleracea (Spinach) Spinach MKIRASVRPICEKCRLIRRRGRIIVICSNPKHKQRQG DA9265284AD21915 rk14_spiol 50S ribosomal protein L14, chloroplastic Chloroplastic large ribosomal subunit protein uL14c Ribosomal protein CS-L29 rpl14 protein protein spiol Spinacia oleracea (Spinach) Spinach MIQPQTHLNVADNSGARELMCIRIIGASNRRYARIGDVIVAVIKEAIPNTPLERSEVIRAVVVRTCKELKRDNGMIIRYDDNAAVIIDQEGNPKGTRIFGAIARELRQKFAKIVSLAPEVL 1C81677D2A35EC22 rk22_spiol 50S ribosomal protein L22, chloroplastic Chloroplastic large ribosomal subunit protein uL22c Ribosomal protein CS-L13 rpl22 protein protein spiol Spinacia oleracea (Spinach) Spinach MGFFKKKEKKAEFDVFRLYGLHHPEPGKCDEITTRGYSISMSVDKARRVIDQIRGRSYAETLMILELMPYRACYPIFKLIYSAAANASHNKQFNKANLIISKAEVNKGITLKKVKPRARGRSYMIKRPTCHITIVLRDITHFDSYDKFLESLTPKKLIALLGLMSTGRRRELLCGRFRENHKIKSFLYKIALFKRYEVM 58C444003AC28436 rk9_spiol 50S ribosomal protein L9, chloroplastic CL9 Chloroplastic large ribosomal subunit protein bL9c SOVF_040960 RPL9 protein protein spiol Spinacia oleracea (Spinach) Spinach MASTTSTLSLSWSNSFHSFAGAISEPQKSPENCRVMLPIVAQKKVKKIRKIILKEDIPDLGKKGQLLDVRAGFLRNFLLPLGKAEVVTPLLLKEMKMEDERIEAEKKRVKEEAQQLARMFETVGAFKVKRKGGKGKQIFGSVTAQDLVDIIKAQLQRDVDKKVVFLPDIRETGEYIAELKLHPDVTAQVRVTVFAN 1823DE61DD7349F8 psrp5-psrp6-3 1 6 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 26 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 22 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 27 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 5 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 13 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 12 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 9 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 23 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 14 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 21 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 4 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 19 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 29 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 24 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 2 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 15 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 3 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 16 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 17 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 20 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 28 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 25 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 30 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 7 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 8 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 18 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 11 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 10 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified physical association physical association Fig 1, Fig S2, Supplementary Table S1