IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Functional Characterization of PRKAR1A Mutations Reveals a Unique Molecular Mechanism Causing Acrodysostosis but Multiple Mechanisms Causing Carney Complex. Functional Characterization of PRKAR1A Mutations Reveals a Unique Molecular Mechanism Causing Acrodysostosis but Multiple Mechanisms Causing Carney Complex. The Journal of biological chemistry 2015 imex curation Rhayem Y., Le Stunff C., Abdel Khalek W., Auzan C., Bertherat J., Linglart A., Couvineau A., Silve C., Clauser E. Rare diseases - Interactions investigated in the context of Rare genetic disease eric.clauser@inserm.fr 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret tag visualisation tag visualisation Rhayem Y., Le Stunff C., Abdel Khalek W., Auzan C., Bertherat J., Linglart A., Couvineau A., Silve C., Clauser E. Rare diseases - Interactions investigated in the context of Rare genetic disease EC50 values provided here are associated with the inhibitors (cAMP analogs) halfway response after a specified exposure time - rather than the targets; mutant-specific EC50 values were translated as mutation effect similar to that of Kd eric.clauser@inserm.fr Accepted 2020-FEB-10 AT 12:51 GMT AT 12:51 GMT by LIVIAP The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation kapca_mouse cAMP-dependent protein kinase catalytic subunit alpha Prkaca Pkaca protein protein mouse Mus musculus Mouse MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWETPSQNTAQLDQFDRIKTLGTGSFGRVMLVKHKESGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVAGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFTEF DIP protein P05132 original sequence version: 185 DIP protein P05132 original sequence version: 186 DIP protein P05132-1 original sequence version: 145 DIP protein P05132-1 original sequence version: 193 DIP protein P05132-1 original sequence version: 199 DIP protein P05132 original sequence version: 163 DIP protein P05132 original sequence version: 192 DIP protein P05132-1 original sequence version: 197 DIP protein P05132-1 original sequence version: 149 02F85D66EB21A1FA kap0_human cAMP-dependent protein kinase type I-alpha regulatory subunit Tissue-specific extinguisher 1 PRKAR1A PKR1 PRKAR1 TSE1 protein protein human Homo sapiens Human MESGSTAASEEARSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNLQKAGTRTDSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFSVSFIAGETVIQQGDEGDNFYVIDQGETDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV DIP protein P10644 original sequence version: 173 DIP protein P10644 original sequence version: 127 DIP protein P10644 original sequence version: 134 DIP protein P10644 original sequence version: 179 2D04F08CE8857A6D 8-aha-camp 8-(6-aminohexylamino)adenosine-3′, 5′-cyclic monophosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) 8-bromo-camp 8-Bromo-cAMP (4aR,6R,7R,7aS)-6-(6-amino-8-bromo-9H-purin-9-yl)tetrahydro-4H-furo[3,2-d][1,3,2]dioxaphosphinine-2,7-diol 2-oxide 8-bromo cyclic adenosine monophosphate 8-bromo-cAMP 8-Bromo-cAMP 8-bromo-cyclic 3',5'-AMP 8-bromo-cyclic AMP 8-bromoadenosine 3',5'-cyclic monophosphate 8-bromoadenosine 3',5'-monophosphate 8-bromoadenosine cyclic 3',5'-phosphate BCAMP cyclic 8-bromoadenosine 3',5'-monophosphate 8-bromoadenosine 3',5'-(hydrogen phosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) 8-pip-camp 8-PIP-cAMP (4aR,6R,7R,7aS)-6-[6-amino-8-(piperidin-1-yl)-9H-purin-9-yl]tetrahydro-4H-furo[3,2-d][1,3,2]dioxaphosphinine-2,7-diol 2-oxide 8-piperidinoadenosine-3',5'-cyclic monophosphate 8-piperidin-1-yladenosine 3',5'-(hydrogen phosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) prkaca-prkar1a-13 1 3 unspecified role unspecified role luminescence donor luminescence donor over-expressed over expressed level over-expressed living cell living cell p.Gln372* mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain QYNSFVSLSV . p.Arg368* mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain RNIQQYNSFVSLSV . P10644:p.Gln285Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Q R Tue Jun 30 19:16:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln285Arg' region renilla luciferase renilla luciferase protein tag renilla luciferase undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P10644:p.Gly289Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G E Tue Jun 30 20:19:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly289Glu' P10644:p.Ala328Val mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A V Tue Jun 30 18:06:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala328Val' P10644:p.Arg335Leu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R L Tue Jun 30 18:47:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg335Leu' P10644:p.Tyr175Cys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Y C Wed Jan 29 12:27:47 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp175Cys' Tue Jun 30 16:58:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr175Cys' P10644:p.Ala213Thr mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A T Tue Jun 30 19:52:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala213Thr' P10644:p.Gly289Trp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G W Tue Jun 30 15:59:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly289Trp' P10644:p.Ala213Asp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A D Tue Jun 30 16:06:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala213Asp' 2 unspecified role unspecified role luminescence accept luminescence acceptor luminescence accept over-expressed over expressed level over-expressed living cell living cell region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined proximity proximity close-contact colocalization neighbourhood interaction FIGURE 3, table 1 8_pip_camp-prkaca-1 1 3 unspecified role unspecified role luminescence donor luminescence donor over-expressed over expressed level over-expressed living cell living cell P10644:p.Gln285Arg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Q R Tue Jun 30 18:48:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln285Arg' region renilla luciferase renilla luciferase protein tag renilla luciferase undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P10644:p.Gly289Glu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G E Tue Jun 30 18:31:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly289Glu' P10644:p.Ala328Val mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A V Tue Jun 30 16:20:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala328Val' P10644:p.Arg335Leu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R L Tue Jun 30 18:25:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg335Leu' P10644:p.Tyr175Cys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y C Wed Jan 29 11:26:23 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp175Cys' Tue Jun 30 18:00:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr175Cys' P10644:p.Ala213Thr mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A T Tue Jun 30 17:34:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala213Thr' p.Gln372* mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain QYNSFVSLSV . p.Arg368* mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain RNIQQYNSFVSLSV . 2 unspecified role unspecified role luminescence accept luminescence acceptor luminescence accept over-expressed over expressed level over-expressed living cell living cell region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 inhibitor inhibitor neutral component neutral component purified purified proximity proximity close-contact colocalization neighbourhood interaction FIGURE 4, Table 2, Figure 7 8_bromo_camp-prkaca-1 1 3 unspecified role unspecified role luminescence donor luminescence donor over-expressed over expressed level over-expressed living cell living cell P10644:p.Gln285Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Q R Tue Jun 30 16:23:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln285Arg' region renilla luciferase renilla luciferase protein tag renilla luciferase undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P10644:p.Gly289Glu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G E Tue Jun 30 19:31:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly289Glu' P10644:p.Ala328Val mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A V Tue Jun 30 19:20:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala328Val' P10644:p.Arg335Leu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R L Tue Jun 30 17:21:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg335Leu' P10644:p.Tyr175Cys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y C Wed Jan 29 12:29:24 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp175Cys' Tue Jun 30 19:54:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr175Cys' P10644:p.Ala213Thr mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A T Tue Jun 30 20:17:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala213Thr' p.Gln372* mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain QYNSFVSLSV . p.Arg368* mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain RNIQQYNSFVSLSV . 2 unspecified role unspecified role luminescence accept luminescence acceptor luminescence accept over-expressed over expressed level over-expressed living cell living cell region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 inhibitor inhibitor neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) proximity proximity close-contact colocalization neighbourhood interaction FIGURE 4, Table 2, Figure 7 8_bromo_camp-prkaca-2 1 3 unspecified role unspecified role luminescence donor luminescence donor over-expressed over expressed level over-expressed living cell living cell P10644:p.Gln285Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Q R Tue Jun 30 15:36:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln285Arg' region renilla luciferase renilla luciferase protein tag renilla luciferase undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P10644:p.Gly289Glu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G E Tue Jun 30 15:39:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly289Glu' P10644:p.Ala328Val mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A V Tue Jun 30 18:49:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala328Val' P10644:p.Arg335Leu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R L Tue Jun 30 19:02:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg335Leu' P10644:p.Tyr175Cys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y C Wed Jan 29 12:07:02 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp175Cys' Tue Jun 30 17:28:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr175Cys' P10644:p.Ala213Thr mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A T Tue Jun 30 17:22:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala213Thr' p.Gln372* mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain QYNSFVSLSV . p.Arg368* mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain RNIQQYNSFVSLSV . 4 inhibitor inhibitor neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 2 unspecified role unspecified role luminescence accept luminescence acceptor luminescence accept over-expressed over expressed level over-expressed living cell living cell region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined proximity proximity close-contact colocalization neighbourhood interaction FIGURE 4, Table 2, Figure 7