IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Functional antagonism between high temperature requirement protein A (HtrA) family members regulates trophoblast invasion. Functional antagonism between high temperature requirement protein A (HtrA) family members regulates trophoblast invasion. The Journal of biological chemistry 2014 imex curation Chen YY., Chuang PY., Chen CP., Chiu YH., Lo HF., Cheong ML., Huang JY., Kuo PL., Chen H. HtrA1, HtrA3, and HtrA4 tagged constructs are of Human origin based on stated residues of "Ser-328, Ser-305, and Ser-326 in HtrA1, HtrA3, and HtrA4 ". hwchen@gate.sinica.edu.tw 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Chen YY., Chuang PY., Chen CP., Chiu YH., Lo HF., Cheong ML., Huang JY., Kuo PL., Chen H. HtrA1, HtrA3, and HtrA4 tagged constructs are of Human origin based on stated residues of "Ser-328, Ser-305, and Ser-326 in HtrA1, HtrA3, and HtrA4 ". hwchen@gate.sinica.edu.tw Accepted 2020-MAR-12 AT 14:01 GMT AT 14:01 GMT by KALPANAP The Journal of biological chemistry 2014 Only protein-protein interactions imex curation imex curation Functional antagonism between high temperature requirement protein A (HtrA) family members regulates trophoblast invasion. Functional antagonism between high temperature requirement protein A (HtrA) family members regulates trophoblast invasion. The Journal of biological chemistry 2014 imex curation Chen YY., Chuang PY., Chen CP., Chiu YH., Lo HF., Cheong ML., Huang JY., Kuo PL., Chen H. HtrA1, HtrA3, and HtrA4 tagged constructs are of Human origin based on stated residues of "Ser-328, Ser-305, and Ser-326 in HtrA1, HtrA3, and HtrA4 ". hwchen@gate.sinica.edu.tw 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro protease assay protease assay western blot western blot Immuno blot Immuno blot Chen YY., Chuang PY., Chen CP., Chiu YH., Lo HF., Cheong ML., Huang JY., Kuo PL., Chen H. GST tagged constructs have been assumed to have been expressed in E.coli, but no information is given as regards purification - or lack of. I have added this experiment as though the tagged constructs are only IP purified there are no other apparent bands visible on WB. HtrA1, HtrA3, and HtrA4 tagged constructs are of Human origin based on stated residues of "Ser-328, Ser-305, and Ser-326 in HtrA1, HtrA3, and HtrA4 ". hwchen@gate.sinica.edu.tw Accepted 2020-MAR-12 AT 14:01 GMT AT 14:01 GMT by KALPANAP The Journal of biological chemistry 2014 Only protein-protein interactions imex curation imex curation htra3_human Serine protease HTRA3 HTRA3 PRSP High-temperature requirement factor A3 Pregnancy-related serine protease protein protein human Homo sapiens Human MQARALLLAALAALALAREPPAAPCPARCDVSRCPSPRCPGGYVPDLCNCCLVCAASEGEPCGGPLDSPCGESLECVRGLCRCRWSHAVCGTDGHTYANVCALQAASRRALQLSGTPVRQLQKGACPLGLHQLSSPRYKFNFIADVVEKIAPAVVHIELFLRHPLFGRNVPLSSGSGFIMSEAGLIITNAHVVSSNSAAPGRQQLKVQLQNGDSYEATIKDIDKKSDIATIKIHPKKKLPVLLLGHSADLRPGEFVVAIGSPFALQNTVTTGIVSTAQREGRELGLRDSDMDYIQTDAIINYGNSGGPLVNLDGEVIGINTLKVTAGISFAIPSDRITRFLTEFQDKQIKDWKKRFIGIRMRTITPSLVDELKASNPDFPEVSSGIYVQEVAPNSPSQRGGIQDGDIIVKVNGRPLVDSSELQEAVLTESPLLLEVRRGNDDLLFSIAPEVVM 3046FCDA1AB24FA6 htra4_human Serine protease HTRA4 High-temperature requirement factor A4 HTRA4 protein protein human Homo sapiens Human MIRPQLRTAGLGRCLLPGLLLLLVPVLWAGAEKLHTQPSCPAVCQPTRCPALPTCALGTTPVFDLCRCCRVCPAAEREVCGGAQGQPCAPGLQCLQPLRPGFPSTCGCPTLGGAVCGSDRRTYPSMCALRAENRAARRLGKVPAVPVQWGNCGDTGTRSAGPLRRNYNFIAAVVEKVAPSVVHVQLWGRLLHGSRLVPVYSGSGFIVSEDGLIITNAHVVRNQQWIEVVLQNGARYEAVVKDIDLKLDLAVIKIESNAELPVLMLGRSSDLRAGEFVVALGSPFSLQNTATAGIVSTKQRGGKELGMKDSDMDYVQIDATINYGNSGGPLVNLDGDVIGVNSLRVTDGISFAIPSDRVRQFLAEYHEHQMKGKAFSNKKYLGLQMLSLTVPLSEELKMHYPDFPDVSSGVYVCKVVEGTAAQSSGLRDHDVIVNINGKPITTTTDVVKALDSDSLSMAVLRGKDNLLLTVIPETIN htra1_human Serine protease HTRA1 HTRA1 HTRA PRSS11 High-temperature requirement A serine peptidase 1 L56 Serine protease 11 protein protein human Homo sapiens Human MQIPRAALLPLLLLLLAAPASAQLSRAGRSAPLAAGCPDRCEPARCPPQPEHCEGGRARDACGCCEVCGAPEGAACGLQEGPCGEGLQCVVPFGVPASATVRRRAQAGLCVCASSEPVCGSDANTYANLCQLRAASRRSERLHRPPVIVLQRGACGQGQEDPNSLRHKYNFIADVVEKIAPAVVHIELFRKLPFSKREVPVASGSGFIVSEDGLIVTNAHVVTNKHRVKVELKNGATYEAKIKDVDEKADIALIKIDHQGKLPVLLLGRSSELRPGEFVVAIGSPFSLQNTVTTGIVSTTQRGGKELGLRNSDMDYIQTDAIINYGNSGGPLVNLDGEVIGINTLKVTAGISFAIPSDKIKKFLTESHDRQAKGKAITKKKYIGIRMMSLTSSKAKELKDRHRDFPDVISGAYIIEVIPDTPAEAGGLKENDVIISINGQSVVSANDVSDVIKRESTLNMVVRRGNEDIMITVIPEEIDP DIP protein Q92743 original sequence version: 106 DIP protein Q92743 original sequence version: 149 DIP protein Q92743 original sequence version: 119 CA20A99480FB2330 htra4-htra1-1 1 3 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed P83110:p.Ser305Ala mutation mutation certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 16:21:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser305Ala' C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed C-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P83105:p.Ser326Ala mutation mutation certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 16:08:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser326Ala' physical association physical association 2A, number 1 htra4-htra1-2 1 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed Q92743:p.Ser328Ala mutation mutation certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 18:16:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser328Ala' C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed C-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P83105:p.Ser326Ala mutation mutation certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 18:18:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser326Ala' physical association physical association 2A, number 2 htra4-htra3-1 1 4 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed P83105:p.Ser326Ala mutation mutation certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 20:23:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser326Ala' C-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed P83110:p.Ser305Ala mutation mutation certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 18:18:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser305Ala' C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 2A, number 3 htra4-htra1-3 1 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed Q92743:p.Ser328Ala mutation mutation certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 17:26:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser328Ala' C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed C-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P83105:p.Ser326Ala mutation mutation certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 15:40:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser326Ala' physical association physical association 2A, number 4 htra4-htra1-4 2 5 enzyme enzyme neutral component neutral component over-expressed over expressed level over-expressed partially purified partially purified Q92743:p.Ser328Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 15:43:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser328Ala' C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 4 enzyme target enzyme target substrate substrate neutral component neutral component over-expressed over expressed level over-expressed partially purified partially purified C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range P83105:p.Ser326Ala mutation mutation certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 16:34:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser326Ala' human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. protein cleavage protein cleavage 3A, number 1 htra1-htra4-1 2 3 enzyme enzyme neutral component neutral component over-expressed over expressed level over-expressed P83110:p.Ser305Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 18:43:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser305Ala' C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 4 enzyme target enzyme target substrate substrate neutral component neutral component over-expressed over expressed level over-expressed partially purified partially purified C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range P83105:p.Ser326Ala mutation mutation certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 16:15:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser326Ala' human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. protein cleavage protein cleavage 3A, number 2 htra1-htra4-2 2 5 enzyme target enzyme target substrate substrate neutral component neutral component over-expressed over expressed level over-expressed partially purified partially purified Q92743:p.Ser328Ala mutation mutation certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 17:58:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser328Ala' C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 4 enzyme enzyme neutral component neutral component over-expressed over expressed level over-expressed partially purified partially purified C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. protein cleavage protein cleavage 3B, number 1 Only slight protease activity was observed. htra1-htra4-3 2 3 enzyme target enzyme target substrate substrate neutral component neutral component over-expressed over expressed level over-expressed partially purified partially purified P83110:p.Ser305Ala mutation mutation certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 20:01:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser305Ala' C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 4 enzyme enzyme neutral component neutral component over-expressed over expressed level over-expressed partially purified partially purified C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. protein cleavage protein cleavage 3B, number 2 Only slight protease activity was observed. htra3-htra4-1 2 4 putative self putative self putative self putative self over-expressed over expressed level over-expressed partially purified partially purified C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. protein cleavage protein cleavage true 3B, number 3,3C HtrA4-FLAG underwent autocatalytic cleavage into lower molecular weight forms. htra4-1 2 5 inhibitor inhibitor neutral component neutral component kazal_domain sufficient to bind sufficient binding region sufficient to bind range range range range n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 4 putative self putative self putative self putative self over-expressed over expressed level over-expressed partially purified partially purified C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. protein cleavage protein cleavage 3C, number 1 htra1-htra4-4 2 3 inhibitor inhibitor neutral component neutral component kazal_domain sufficient to bind sufficient binding region sufficient to bind range range range range n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 4 putative self putative self putative self putative self over-expressed over expressed level over-expressed partially purified partially purified C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. protein cleavage protein cleavage 3C, number 2 htra3-htra4-2 2 4 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed partially purified partially purified C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 4 unspecified role unspecified role unspecified role unspecified role kazal_domain sufficient to bind sufficient binding region sufficient to bind range range range range n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) protein cleavage protein cleavage 3C, number 3 left roles all as "unspecified" as no idea what is doing what here.