MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it The Haemophilus ducreyi LspA1 protein inhibits phagocytosis by using a new mechanism involving activation of C-terminal Src kinase. The Haemophilus ducreyi LspA1 protein inhibits phagocytosis by using a new mechanism involving activation of C-terminal Src kinase. mBio 2014 imex curation Dodd DA., Worth RG., Rosen MK., Grinstein S., van Oers NS., Hansen EJ. Hansen@UTSouthwestern.edu 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro protein kinase assay protein kinase assay antibody detection identification by antibody antibody detection Dodd DA., Worth RG., Rosen MK., Grinstein S., van Oers NS., Hansen EJ. Hansen@UTSouthwestern.edu Accepted 2020-NOV-12 AT 15:30 GMT AT 15:30 GMT by LUANA mBio 2014 Only protein-protein interactions imex curation imex curation The Haemophilus ducreyi LspA1 protein inhibits phagocytosis by using a new mechanism involving activation of C-terminal Src kinase. The Haemophilus ducreyi LspA1 protein inhibits phagocytosis by using a new mechanism involving activation of C-terminal Src kinase. mBio 2014 imex curation Dodd DA., Worth RG., Rosen MK., Grinstein S., van Oers NS., Hansen EJ. Hansen@UTSouthwestern.edu 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. pull down pull down ms participant Identification by mass spectrometry ms participant Dodd DA., Worth RG., Rosen MK., Grinstein S., van Oers NS., Hansen EJ. Hansen@UTSouthwestern.edu Accepted 2020-NOV-12 AT 15:30 GMT AT 15:30 GMT by LUANA mBio 2014 Only protein-protein interactions imex curation imex curation The Haemophilus ducreyi LspA1 protein inhibits phagocytosis by using a new mechanism involving activation of C-terminal Src kinase. The Haemophilus ducreyi LspA1 protein inhibits phagocytosis by using a new mechanism involving activation of C-terminal Src kinase. mBio 2014 imex curation Dodd DA., Worth RG., Rosen MK., Grinstein S., van Oers NS., Hansen EJ. Hansen@UTSouthwestern.edu 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. pull down pull down anti tag western anti tag western blot anti tag western Dodd DA., Worth RG., Rosen MK., Grinstein S., van Oers NS., Hansen EJ. Hansen@UTSouthwestern.edu Accepted 2020-NOV-12 AT 15:30 GMT AT 15:30 GMT by LUANA mBio 2014 Only protein-protein interactions imex curation imex curation nono_human Non-POU domain-containing octamer-binding protein NONO NRB54 54 kDa nuclear RNA- and DNA-binding protein p54(nrb) 55 kDa nuclear protein NMT55 DNA-binding p52/p100 complex, 52 kDa subunit protein protein human Homo sapiens Human MQSNKTFNLEKQNHTPRKHHQHHHQQQHHQQQQQQPPPPPIPANGQQASSQNEGLTIDLKNFRKPGEKTFTQRSRLFVGNLPPDITEEEMRKLFEKYGKAGEVFIHKDKGFGFIRLETRTLAEIAKVELDNMPLRGKQLRVRFACHSASLTVRNLPQYVSNELLEEAFSVFGQVERAVVIVDDRGRPSGKGIVEFSGKPAARKALDRCSEGSFLLTTFPRPVTVEPMDQLDDEEGLPEKLVIKNQQFHKEREQPPRFAQPGSFEYEYAMRWKALIEMEKQQQDQVDRNIKEAREKLEMEMEAARHEHQVMLMRQDLMRRQEELRRMEELHNQEVQKRKQLELRQEEERRRREEEMRRQQEEMMRRQQEGFKGTFPDAREQEIRMGQMAMGGAMGINNRGAMPPAPVPAGTPAPPGPATMMPDGTLGLTPPTTERFGQAATMEGIGAIGGTPPAFNRAAPGAEFAPNKRRRY DIP protein Q15233 original sequence version: 156 DIP protein Q15233 original sequence version: 148 DIP protein Q15233 original sequence version: 139 DIP protein Q15233 original sequence version: 91 26BBD3828F5B9E49 dhx9_human ATP-dependent RNA helicase A DHX9 DDX9 NDH2 DExH-box helicase 9 RNA helicase A LKP Nuclear DNA helicase II DEAH box protein 9 Leukophysin protein protein human Homo sapiens Human MGDVKNFLYAWCGKRKMTPSYEIRAVGNKNRQKFMCEVQVEGYNYTGMGNSTNKKDAQSNAARDFVNYLVRINEIKSEEVPAFGVASPPPLTDTPDTTANAEGDLPTTMGGPLPPHLALKAENNSEVGASGYGVPGPTWDRGANLKDYYSRKEEQEVQATLESEEVDLNAGLHGNWTLENAKARLNQYFQKEKIQGEYKYTQVGPDHNRSFIAEMTIYIKQLGRRIFAREHGSNKKLAAQSCALSLVRQLYHLGVVEAYSGLTKKKEGETVEPYKVNLSQDLEHQLQNIIQELNLEILPPPEDPSVPVALNIGKLAQFEPSQRQNQVGVVPWSPPQSNWNPWTSSNIDEGPLAFATPEQISMDLKNELMYQLEQDHDLQAILQERELLPVKKFESEILEAISQNSVVIIRGATGCGKTTQVPQFILDDFIQNDRAAECNIVVTQPRRISAVSVAERVAFERGEEPGKSCGYSVRFESILPRPHASIMFCTVGVLLRKLEAGIRGISHVIVDEIHERDINTDFLLVVLRDVVQAYPEVRIVLMSATIDTSMFCEYFFNCPIIEVYGRTYPVQEYFLEDCIQMTHFVPPPKDKKKKDKDDDGGEDDDANCNLICGDEYGPETRLSMSQLNEKETPFELIEALLKYIETLNVPGAVLVFLPGWNLIYTMQKHLEMNPHFGSHRYQILPLHSQIPREEQRKVFDPVPVGVTKVILSTNIAETSITINDVVYVIDSCKQKVKLFTAHNNMTNYATVWASKTNLEQRKGRAGRVRPGFCFHLCSRARFERLETHMTPEMFRTPLHEIALSIKLLRLGGIGQFLAKAIEPPPLDAVIEAEHTLRELDALDANDELTPLGRILAKLPIEPRFGKMMIMGCIFYVGDAICTIAAATCFPEPFINEGKRLGYIHRNFAGNRFSDHVALLSVFQAWDDARMGGEEAEIRFCEHKRLNMATLRMTWEAKVQLKEILINSGFPEDCLLTQVFTNTGPDNNLDVVISLLAFGVYPNVCYHKEKRKILTTEGRNALIHKSSVNCPFSSQDMKYPSPFFVFGEKIRTRAISAKGMTLVTPLQLLLFASKKVQSDGQIVLVDDWIKLQISHEAAACITGLRAAMEALVVEVTKQPAIISQLDPVNERMLNMIRQISRPSAAGINLMIGSTRYGDGPRPPKMARYDNGSGYRRGGSSYSGGGYGGGYSSGGYGSGGYGGSANSFRAGYGAGVGGGYRGVSRGGFRGNSGGDYRGPSGGYRGSGGFQRGGGRGAYGTGYFGQGRGGGGY DIP protein Q08211 original sequence version: 114 DIP protein Q08211 original sequence version: 177 A607DA8F4C4B217A dhx15_human Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 DHX15 DBP1 DDX15 DEAH box protein 15 ATP-dependent RNA helicase #46 protein protein human Homo sapiens Human MSKRHRLDLGEDYPSGKKRAGTDGKDRDRDRDREDRSKDRDRERDRGDREREREKEKEKELRASTNAMLISAGLPPLKASHSAHSTHSAHSTHSTHSAHSTHAGHAGHTSLPQCINPFTNLPHTPRYYDILKKRLQLPVWEYKDRFTDILVRHQSFVLVGETGSGKTTQIPQWCVEYMRSLPGPKRGVACTQPRRVAAMSVAQRVADEMDVMLGQEVGYSIRFEDCSSAKTILKYMTDGMLLREAMNDPLLERYGVIILDEAHERTLATDILMGVLKEVVRQRSDLKVIVMSATLDAGKFQIYFDNCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHMCEEEEGDLLLFLTGQEEIDEACKRIKREVDDLGPEVGDIKIIPLYSTLPPQQQQRIFEPPPPKKQNGAIGRKVVVSTNIAETSLTIDGVVFVIDPGFAKQKVYNPRIRVESLLVTAISKASAQQRAGRAGRTRPGKCFRLYTEKAYKTEMQDNTYPEILRSNLGSVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALNDDGDLTELGSMMAEFPLDPQLAKMVIASCDYNCSNEVLSITAMLSVPQCFVRPTEAKKAADEAKMRFAHIDGDHLTLLNVYHAFKQNHESVQWCYDNFINYRSLMSADNVRQQLSRIMDRFNLPRRSTDFTSRDYYINIRKALVTGYFMQVAHLERTGHYLTVKDNQVVQLHPSTVLDHKPEWVLYNEFVLTTKNYIRTCTDIKPEWLVKIAPQYYDMSNFPQCEAKRQLDRIIAKLQSKEYSQY DIP protein O43143 original sequence version: 101 DIP protein O43143 original sequence version: 157 9A21FBE0051CCAA9 nck1_human Cytoplasmic protein NCK1 NCK1 NCK NCK adaptor protein 1 SH2/SH3 adaptor protein NCK-alpha protein protein human Homo sapiens Human MAEEVVVVAKFDYVAQQEQELDIKKNERLWLLDDSKSWWRVRNSMNKTGFVPSNYVERKNSARKASIVKNLKDTLGIGKVKRKPSVPDSASPADDSFVDPGERLYDLNMPAYVKFNYMAEREDELSLIKGTKVIVMEKCSDGWWRGSYNGQVGWFPSNYVTEEGDSPLGDHVGSLSEKLAAVVNNLNTGQVLHVVQALYPFSSSNDEELNFEKGDVMDVIEKPENDPEWWKCRKINGMVGLVPKNYVTVMQNNPLTSGLEPSPPQCDYIRPSLTGKFAGNPWYYGKVTRHQAEMALNERGHEGDFLIRDSESSPNDFSVSLKAQGKNKHFKVQLKETVYCIGQRKFSTMEELVEHYKKAPIFTSEQGEKLYLVKHLS 554E9B1A936AEF30 cdk1_human Cyclin-dependent kinase 1 p34 protein kinase Cell division protein kinase 1 CDK1 CDC2 Cell division control protein 2 homolog CDC28A CDKN1 P34CDC2 protein protein human Homo sapiens Human MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRHPNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM DIP protein P06493 original sequence version: 185 DIP protein P06493 original sequence version: 162 DIP protein P06493 original sequence version: 184 DIP protein P06493 original sequence version: 97 DIP protein P06493 original sequence version: 192 942D79448EFE490A grb2_human Growth factor receptor-bound protein 2 GRB2 ASH Adapter protein GRB2 SH2/SH3 adapter GRB2 Protein Ash protein protein human Homo sapiens Human MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV 83A4B0BA1B248DC4 csk_human Tyrosine-protein kinase CSK CSK C-Src kinase Protein-tyrosine kinase CYL protein protein human Homo sapiens Human MSAIQAAWPSGTECIAKYNFHGTAEQDLPFCKGDVLTIVAVTKDPNWYKAKNKVGREGIIPANYVQKREGVKAGTKLSLMPWFHGKITREQAERLLYPPETGLFLVRESTNYPGDYTLCVSCDGKVEHYRIMYHASKLSIDEEVYFENLMQLVEHYTSDADGLCTRLIKPKVMEGTVAAQDEFYRSGWALNMKELKLLQTIGKGEFGDVMLGDYRGNKVAVKCIKNDATAQAFLAEASVMTQLRHSNLVQLLGVIVEEKGGLYIVTEYMAKGSLVDYLRSRGRSVLGGDCLLKFSLDVCEAMEYLEGNNFVHRDLAARNVLVSEDNVAKVSDFGLTKEASSTQDTGKLPVKWTAPEALREKKFSTKSDVWSFGILLWEIYSFGRVPYPRIPLKDVVPRVEKGYKMDAPDGCPPAVYEVMKNCWHLDAAMRPSFLQLREQLEHIKTHELHL 431023A88C54E00C sfpq_human Splicing factor, proline- and glutamine-rich SFPQ PSF Polypyrimidine tract-binding protein-associated-splicing factor DNA-binding p52/p100 complex, 100 kDa subunit 100 kDa DNA-pairing protein protein protein human Homo sapiens Human MSRDRFRSRGGGGGGFHRRGGGGGRGGLHDFRSPPPGMGLNQNRGPMGPGPGQSGPKPPIPPPPPHQQQQQPPPQQPPPQQPPPHQPPPHPQPHQQQQPPPPPQDSSKPVVAQGPGPAPGVGSAPPASSSAPPATPPTSGAPPGSGPGPTPTPPPAVTSAPPGAPPPTPPSSGVPTTPPQAGGPPPPPAAVPGPGPGPKQGPGPGGPKGGKMPGGPKPGGGPGLSTPGGHPKPPHRGGGEPRGGRQHHPPYHQQHHQGPPPGGPGGRSEEKISDSEGFKANLSLLRRPGEKTYTQRCRLFVGNLPADITEDEFKRLFAKYGEPGEVFINKGKGFGFIKLESRALAEIAKAELDDTPMRGRQLRVRFATHAAALSVRNLSPYVSNELLEEAFSQFGPIERAVVIVDDRGRSTGKGIVEFASKPAARKAFERCSEGVFLLTTTPRPVIVEPLEQLDDEDGLPEKLAQKNPMYQKERETPPRFAQHGTFEYEYSQRWKSLDEMEKQQREQVEKNMKDAKDKLESEMEDAYHEHQANLLRQDLMRRQEELRRMEELHNQEMQKRKEMQLRQEEERRRREEEMMIRQREMEEQMRRQREESYSRMGYMDPRERDMRMGGGGAMNMGDPYGSGGQKFPPLGGGGGIGYEANPGVPPATMSGSMMGSDMRTERFGQGGAGPVGGQGPRGMGPGTPAGYGRGREEYEGPNKKPRF DIP protein P23246 original sequence version: 182 6D8D5EA95E235847 q7vle8_haedu lspA1 HD_1505 protein protein haedu Haemophilus ducreyi (strain 35000HP / ATCC 700724) MNNKRYKLIFSKVKNCLVPVAENIKSASGNSGSSSNSKIAEDQEEEPDSLACSLSPLSSSIHLGLHNHSPLKVFKGKSLSVVLLSLMPATPLLAQQNYAEALNGKVYVDSQHSSTRIYEQKTNDNSKDGIVVVEIANPEVDGVSDNRFKEFNIPNSAVFNNSRTESTSQLVGKLHANIQLQKEAKLILNQVTGDHESNIQGALEVAGKKADLIIVNPNGITLNGVKTINTDRFVVSTSDIIPHRENGLLSVRNGKVTIDKGGVATNGLSHFEVVARNIDQKGKITVAKTENQKSVNPANITFAAGSLNYNLKTREATPISSGTSRTSDTPAISADSAGSMYGSNIKFVVTDKGAGVKHKGIIFSENDINIKMDGGNASLKELYAKKDIDILAKDIELTEKGQLQANNKIILNSTGKINLRNASEVSADNVNVKSENLALENASMSANSLDVIVTKIEVNRSSKVSAGTANIKASNITLDGSSVVANKITLNVTNNATLNNQSKLSAKDMELNVTHNITLNNTSKLSAQKANIKTENLTLNGEASLVAEKLDINAIDKITNNGTIAGLTANITTKALENRDNALILAHQNLNFTVNGSHYVNKGDIVSKDKAIVTFSNNSDFTSNGSKLVDAQNNLTVNVNNFNITQGSEIILHGNVTLNAKGNFTNSGNLTTMKELNISNIESFINAGNLTTGKNLEVHSNTTVKNDGKLVSIENLNISSKTDFTNNGTLLGLEALKIASGGNFTNASNGSLASNKSLDIYGNNFTNNGTIESVKSLNITNNYTFINNATIKSYGVLNITSQGNFTNDSNGTVMSHDLLNITSQANIINKNLLAGGQGLNLTAKGNITNDSNSTAIAVLHSNNDINLNANNKVYNIGEIYSQAGNISVEAKLLHNDVKLSGNITTTTKSGNATVKTNSIGGGLHDANSIRVGELTLNGKFADLDNQLKVALRGKIYAGSNLTFKAKEGEKEQKSTAQAKIINRGTINVKNKLEYGSNVDVENNMRSMQVNLYEKIFNGDNPITLTLKNGVTFAKDFSNRRRRASNDGEGTNKKTFDNVAHLIEEAFSGYSNGNDHRASDDGHVKSPYYLLVLAQAVNNTEGENYLKTALQHIFGPNWNDLTTTNNDTTINDKWNQLKLKWEKFKNNGENNHSINLNIYPADEGVEKAKIFAGVLRNGTNGVEDKVYQELNDKAKKEYEDKFAKKFQGRFKSRFQNGEFDWAGDWAKEGNESYGSKETEEKYNGIKKEHTVNIGKHEIKVPTVSFENLNNINHQQDKSDGIDKSIISELLAQPIYVAKADVPDVDPRVAQNDKAVDEDGLYRTRLSYINQNNYLGAKYFFNQLDTEDDKLKGIKRIGDNYFEHQLITRLIEKVADNHLTLKHGLHDIALVKKLIDSASIQAKDLNLKVGEALTKEQKDNLKEDIVWYVKTEVNGQEVLVPQVYLAKQTIEEVEKQRGVGTGQIRAGIIDVKVDDVRNTGTIAGYAVGLEAKNKLKNTGDILSQRLSKLVGKKGLESTGVTYVDETGATKVRKARIKSEGHIYLETDKDKNVDLTASELKGNTGQIKAKDLNLNDIYETSYKYKYEKLFGKNGGEIGDRVTQTSQAKSVGTDASFDHLHLSLEGDVNQTGSNLKANRTTGVVKGDFNTKAGKDLFHRQIDTVTSGTVYSASASGGGQSAGISLTDQGVETYTNKTATAGANADVTNFMKRTRETETSLTHRNSEFNALSGELYVMGKADIGGVDINRDVEVIKTPEEIAAEQKAAEEAKKAEVKENEASETAAKETEEAENDNVAEKDKTKPKFKKLTDEEIAAAFETKGEDFFAAYKAREEEDRKKGFTLSAEQIESTKARDEKETTYYELKVGVGAEAEAHSAAADAISNKARQIIDTQNGLKQDGTVALQEASDVLNLATGDLAGASAKLKFELSTIEKKSRGASDGRSILGGRLNLAARGGDITLNNVETTENSHLSLKARDNVNVNSGVTEQKDESNSQSLKVTAGASSGCGVMAGGCSAGVSAGVSGSYNESNTESTSHTNSLLRGKSLRVEAGKDFNLISSNVDVDHLHLDVKGDTNVVSKQDSYSRKERGVNYSVSAGVGVSTAGGARPNGSVGLGVSAENENSKIVKQQAGISAKRITGEINNLNLTGGYIENKGNPDELNVKGDITTHELKDEHHKDGGSFGGSVGVSETGVTQVNVNGGRVEQKHYEATQHSSISGINTKGKTVGNFKTDRSQSTEVHRDDTIAATNFNFELGDIAELAKKGKEKWDNRSAKTTSSSQDSAHDPRSRSVENGYSELPRFKTADNDAGVDSPRLIKGEAEQAQTLALTKAGNDVIPEVQSLTQKARPQSLVDESPYAEIPALTRPQVKSNIAESIEVPQFRTKVSDGDEGNYAEITFPTNKAAISSTQDVGDTPTPRALRLESESGYESAENLGLIPRGFKSSPKGEYEDISDAIEPQTRSRKLDEPEPIYGTINKSPEAIARANAKADEAIQALGYDPRIKPVVPEEAPPALPPRNLQTKAISDYDDVSYVPDFKVRKTDEPEPIYGTINKSPEAIARANAKADEAIQASGYDPRIKPVVPEDAPPALPPRTQSLIDSTEVPSYRSALANVKFDDASPWPQPSALRSKAFADEPSSETPKSRGKRGISEESLSSTVQPRSRKISEEDSSFERLPLRIIDNGSDYAEILPRNVKQTNEPATQAIRAPKALDNNDVIAERPSFKLRQLDDDVESVNGIYSSIKPKALIEEGTPITRQVKTVQEETPVTDLVNKRELVKEDRSLLDKVQDTFQPLKVRSKINDVRSSVEEYGGEVTFKYAQSKGEVYNEIVKHAETQNGVCEATCSHWIAKKVNDENIWTDLYKDGQKGRKGGLNKDAIESIEKLQTEFINAGTATQQFKLTNTWLEEQGVVPKQKYFGKLSRADEVAGTVSKNDVSALVKAILDTGNESSAVKKISINLEGGSHTVSASIEGQKVVFFDPNFGEITFKDKKSFEKWMKNAFWKKSGYAGKKDTKRFFNVVNYHKNSKRNKVIDVNQNHIQQLAGSEGFSPSLPTRPQLANAAGIKSNEMSSLFSWSKLKHLFSRESGKKAQVEGPEIKHLGGVVDKDAFYFPLDKIVTRRDAEGEIRVNMDNIKKAFNPRDKHYNSQEARSLRSLYNQDPSMSGTRFIIENQVIANPFSSADLQSYIQAQQSKLPELGRQARRALPELPTAANKGRGSRVEEQNIVTRPRVEDVYATVNKGAKHGEAQQPGSFYTKKLVDQVSHVPNTEPVYADLHFNRNGRVVRQTEPEVIYEKIRGQQVEVDDPSSLYAKVNRNRRLDNVEGFYPPEQLRTRSDKLAEQVSRVPTTEPVYADLRFKSAEDDYAPALPARPELGNAAGFRKAKVKGEESESTWSRLKHLFSRESGKTKVEEVENEYKSQTNGENSLEIKTIEHIPTRLKNLESPREAQSDLGENALIYGLQRGRQALISKANAADKEGKNAILADSYIGKLNLGFEFGELTKFAKQVKDGKVTEQDIQNIASFNDETAKLARRSEPKNRINDANVDDNQRIIRELINNEAAVDALKRIATLSDQEKAMHSTLRANEKFDMDELEESPNYTTAENKSIRDYKDTQKALNDARMDFFTEKTKFIAKETLERGGQLYFALDGLVTNSPGFRADTQINMDKLKDVFNPNHEHYDSVTSRELRYLYENYKDNPNLKFTLKDHVIANPLKTLKTSISESDLKSSPRRARQEGPSLLQRVRNLFDKSSSNKRSEKDTAQTSVGYRNTNIDIINDKTKGVNHIVENGVEVALTNTDYKPLKLPNVEAAFKQTKLKAENIDPHIEAVKKLEIIASSANSIPKEHLLKALIEVTEGKTDEDINVYQKLFNTRQNISNEVAPTYSLRNLDGKDGKQILRSVAEIYKNLPLSDTYQAVRNYVNNRLIEKLSSNRLLLEHLANSKISGNEYAIKYIFDTVSRAKQEIFEQELNTELAPVSLDIMRRKAANILSSEHGSYKDGTLSIYDKPVKSAFHSRLKNNGEVLNTIVHELTHHEQDALAKIIDNKGYDAKLFDKNNILYITGGLGYPKQALERDAFLSGDSVSEAFMKKAKEYHERTKQERKDAKKDEARIAKLYKQWEQEEANKKSASLNGSSQSLDSRSEVEFNRVSHKSVR 3AE20AC173685629 rs23_human 40S ribosomal protein S23 RPS23 Small ribosomal subunit protein uS12 protein protein human Homo sapiens Human MGKCRGLRTARKLRSHRRDQKWHDKQYKKAHLGTALKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKKITAFVPNDGCLNFIEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLLALYKGKKERPRS 8417A48B8CF0A8E5 ptn11_human Tyrosine-protein phosphatase non-receptor type 11 PTPN11 PTP2C SHPTP2 Protein-tyrosine phosphatase 2C Protein-tyrosine phosphatase 1D SH-PTP3 SH-PTP2 protein protein human Homo sapiens Human MTSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQNTGDYYDLYGGEKFATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCADPTSERWFHGHLSGKEAEKLLTEKGKHGSFLVRESQSHPGDFVLSVRTGDDKGESNDGKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHYKKNPMVETLGTVLQLKQPLNTTRINAAEIESRVRELSKLAETTDKVKQGFWEEFETLQQQECKLLYSRKEGQRQENKNKNRYKNILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENSRVIVMTTKEVERGKSKCVKYWPDEYALKEYGVMRVRNVKESAAHDYTLRELKLSKVGQGNTERTVWQYHFRTWPDHGVPSDPGGVLDFLEEVHHKQESIMDAGPVVVHCSAGIGRTGTFIVIDILIDIIREKGVDCDIDVPKTIQMVRSQRSGMVQTEAQYRFIYMAVQHYIETLQRRIEEEQKSKRKGHEYTNIKYSLADQTSGDQSPLPPCTPTPPCAEMREDSARVYENVGLMQQQKSFR 9CDBEFFA5E6CCB45 nck2_human Cytoplasmic protein NCK2 NCK adaptor protein 2 SH2/SH3 adaptor protein NCK-beta NCK2 GRB4 Growth factor receptor-bound protein 4 protein protein human Homo sapiens Human MTEEVIVIAKWDYTAQQDQELDIKKNERLWLLDDSKTWWRVRNAANRTGYVPSNYVERKNSLKKGSLVKNLKDTLGLGKTRRKTSARDASPTPSTDAEYPANGSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVTVMEKCSDGWWRGSYNGQIGWFPSNYVLEEVDEAAAESPSFLSLRKGASLSNGQGSRVLHVVQTLYPFSSVTEEELNFEKGETMEVIEKPENDPEWWKCKNARGQVGLVPKNYVVVLSDGPALHPAHAPQISYTGPSSSGRFAGREWYYGNVTRHQAECALNERGVEGDFLIRDSESSPSDFSVSLKASGKNKHFKVQLVDNVYCIGQRRFHTMDELVEHYKKAPIFTSEHGEKLYLVRALQ 3A211085E46F4452 capr1_human Caprin-1 CAPRIN1 GPIAP1 GPIP137 M11S1 Cytoplasmic activation- and proliferation-associated protein 1 GPI-anchored membrane protein 1 GPI-anchored protein p137 Cell cycle-associated protein 1 Membrane component chromosome 11 surface marker 1 RNG105 RNA granule protein 105 protein protein human Homo sapiens Human MPSATSHSGSGSKSSGPPPPSGSSGSEAAAGAGAAAPASQHPATGTGAVQTEAMKQILGVIDKKLRNLEKKKGKLDDYQERMNKGERLNQDQLDAVSKYQEVTNNLEFAKELQRSFMALSQDIQKTIKKTARREQLMREEAEQKRLKTVLELQYVLDKLGDDEVRTDLKQGLNGVPILSEEELSLLDEFYKLVDPERDMSLRLNEQYEHASIHLWDLLEGKEKPVCGTTYKVLKEIVERVFQSNYFDSTHNHQNGLCEEEEAASAPAVEDQVPEAEPEPAEEYTEQSEVESTEYVNRQFMAETQFTSGEKEQVDEWTVETVEVVNSLQQQPQAASPSVPEPHSLTPVAQADPLVRRQRVQDLMAQMQGPYNFIQDSMLDFENQTLDPAIVSAQPMNPTQNMDMPQLVCPPVHSESRLAQPNQVPVQPEATQVPLVSSTSEGYTASQPLYQPSHATEQRPQKEPIDQIQATISLNTDQTTASSSLPAASQPQVFQAGTSKPLHSSGINVNAAPFQSMQTVFNMNAPVPPVNEPETLKQQNQYQASYNQSFSSQPHQVEQTELQQEQLQTVVGTYHGSPDQSHQVTGNHQQPPQQNTGFPRSNQPYYNSRGVSRGGSRGARGLMNGYRGPANGFRGGYDGYRPSFSNTPNSGYTQSQFSAPRDYSGYQRDGYQQNFKRGSGQSGPRGAPRGRGGPPRPNRGMPQMNTQQVN 56F5BC188CA3A2D4 1-172 2 16 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 6 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 4 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 11 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 13 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 5 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 14 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 7 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 15 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 12 predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant unspecified role unspecified role bait bait over-expressed over expressed level over-expressed myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain association association table 1 lspa1-csk-1 3 12 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed strep tag strep tag strep tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q7VLE8:p.[Tyr2469Phe;Tyr2545Phe] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F 10 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding region binding region binding-associated region binding region range range range range physical association physical association fig. 3 a-e lspa1-csk-2 3 12 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed strep tag strep tag strep tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q7VLE8:p.[Tyr2469Phe;Tyr2545Phe] mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F 15 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association fig. 3b lspa1-nck2-1 3 12 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed strep tag strep tag strep tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q7VLE8:p.[Tyr2469Phe;Tyr2545Phe] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association fig. 3c lspa1-csk-3 3 12 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed strep tag strep tag strep tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q7VLE8:p.[Tyr2469Phe;Tyr2545Phe] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F 10 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding region binding region binding-associated region binding region range range range range physical association physical association fig. 3 e cdk1-csk-1 1 12 stimulator stimulator neutral component neutral component binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q7VLE8:p.[Tyr2469Phe;Tyr2545Phe] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F ecolx Escherichia coli 8 enzyme target enzyme target substrate substrate neutral component neutral component binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 10 enzyme enzyme neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation fig. 5a To investigate whether YL2 could directly activate Csk, a tyrosine kinase assay that measures phosphorylation of a poly (Glu-Tyr) peptide was performed with recombinant Csk and recombinant YL2, YL2-DM, or L1. Increasing concentrations of YL2 increased Csk activity in a dose-dependent manner (Fig. 5). When 65 nM Csk and 50 nM YL2 were incubated together, therewas a 10-fold increase in Csk activity (Fig. 5A). YL2 itself had no kinase activity, and YL2-DM did not stimulate Csk activity cdk1-csk-2 1 12 enzyme target enzyme target substrate substrate neutral component neutral component binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 enzyme enzyme neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation fig. 5b To investigate whether YL2 could directly activate Csk, a tyrosine kinase assay that measures phosphorylation of a poly (Glu-Tyr) peptide was performed with recombinant Csk and recombinant YL2, YL2-DM, or L1. Increasing concentrations of YL2 increased Csk activity in a dose-dependent manner (Fig. 5). When 65 nM Csk and 50 nM YL2 were incubated together, therewas a 10-fold increase in Csk activity (Fig. 5A). YL2 itself had no kinase activity, and YL2-DM did not stimulate Csk activity