IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Proteomic identification of calumenin as a G551D-CFTR associated protein. Proteomic identification of calumenin as a G551D-CFTR associated protein. PloS one 2011 imex curation Teng L., Kerbiriou M., Taiya M., Le Hir S., Mignen O., Benz N., Trouvé P., Férec C. Rare diseases - Interactions investigated in the context of Rare genetic disease pascal.trouve@univ-brest.fr,claude.ferec@univ-brest.fr 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Teng L., Kerbiriou M., Taiya M., Le Hir S., Mignen O., Benz N., Trouvé P., Férec C. Rare diseases - Interactions investigated in the context of Rare genetic disease pascal.trouve@univ-brest.fr,claude.ferec@univ-brest.fr Accepted 2020-MAR-06 AT 08:43 GMT AT 08:43 GMT by MGT PloS one 2011 Only protein-protein interactions imex curation imex curation Proteomic identification of calumenin as a G551D-CFTR associated protein. Proteomic identification of calumenin as a G551D-CFTR associated protein. PloS one 2011 imex curation Teng L., Kerbiriou M., Taiya M., Le Hir S., Mignen O., Benz N., Trouvé P., Férec C. Rare diseases - Interactions investigated in the context of Rare genetic disease pascal.trouve@univ-brest.fr,claude.ferec@univ-brest.fr 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 pull down pull down sequence tag sequence tag identification sequence tag Teng L., Kerbiriou M., Taiya M., Le Hir S., Mignen O., Benz N., Trouvé P., Férec C. Rare diseases - Interactions investigated in the context of Rare genetic disease The protein complexes were subjected to 2D-gel electrophoresis before sequence tag pascal.trouve@univ-brest.fr,claude.ferec@univ-brest.fr Accepted 2020-MAR-06 AT 08:43 GMT AT 08:43 GMT by MGT PloS one 2011 Only protein-protein interactions imex curation imex curation Proteomic identification of calumenin as a G551D-CFTR associated protein. Proteomic identification of calumenin as a G551D-CFTR associated protein. PloS one 2011 imex curation Teng L., Kerbiriou M., Taiya M., Le Hir S., Mignen O., Benz N., Trouvé P., Férec C. Rare diseases - Interactions investigated in the context of Rare genetic disease pascal.trouve@univ-brest.fr,claude.ferec@univ-brest.fr 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Teng L., Kerbiriou M., Taiya M., Le Hir S., Mignen O., Benz N., Trouvé P., Férec C. Rare diseases - Interactions investigated in the context of Rare genetic disease Purified CFTR construct gifted by Pr Robert Ford (University of Manchester, UK), was inferred to be human from pmid 26850167 pascal.trouve@univ-brest.fr,claude.ferec@univ-brest.fr Accepted 2020-MAR-06 AT 08:43 GMT AT 08:43 GMT by MGT PloS one 2011 Only protein-protein interactions imex curation imex curation o43852-pro_0000004153 Calumenin Crocalbin IEF SSP 9302 CALU protein protein human Homo sapiens Human KPTEKKDRVHHEPQLSDKVHNDAQSFDYDHDAFLGAEEAKTFDQLTPEESKERLGKIVSKIDGDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFGEALVRHDEF 20 315 FEB4D3B67910D845 o43852-1 Calumenin CALU Crocalbin IEF SSP 9302 protein protein human Homo sapiens Human MDLRQFLMCLSLCTAFALSKPTEKKDRVHHEPQLSDKVHNDAQSFDYDHDAFLGAEEAKTFDQLTPEESKERLGKIVSKIDGDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFGEALVRHDEF 25BAE5A99B527375 cftr_human Cystic fibrosis transmembrane conductance regulator CFTR ABCC7 cAMP-dependent chloride channel ATP-binding cassette sub-family C member 7 Channel conductance-controlling ATPase protein protein human Homo sapiens Human MQRSPLEKASVVSKLFFSWTRPILRKGYRQRLELSDIYQIPSVDSADNLSEKLEREWDRELASKKNPKLINALRRCFFWRFMFYGIFLYLGEVTKAVQPLLLGRIIASYDPDNKEERSIAIYLGIGLCLLFIVRTLLLHPAIFGLHHIGMQMRIAMFSLIYKKTLKLSSRVLDKISIGQLVSLLSNNLNKFDEGLALAHFVWIAPLQVALLMGLIWELLQASAFCGLGFLIVLALFQAGLGRMMMKYRDQRAGKISERLVITSEMIENIQSVKAYCWEEAMEKMIENLRQTELKLTRKAAYVRYFNSSAFFFSGFFVVFLSVLPYALIKGIILRKIFTTISFCIVLRMAVTRQFPWAVQTWYDSLGAINKIQDFLQKQEYKTLEYNLTTTEVVMENVTAFWEEGFGELFEKAKQNNNNRKTSNGDDSLFFSNFSLLGTPVLKDINFKIERGQLLAVAGSTGAGKTSLLMVIMGELEPSEGKIKHSGRISFCSQFSWIMPGTIKENIIFGVSYDEYRYRSVIKACQLEEDISKFAEKDNIVLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKEIFESCVCKLMANKTRILVTSKMEHLKKADKILILHEGSSYFYGTFSELQNLQPDFSSKLMGCDSFDQFSAERRNSILTETLHRFSLEGDAPVSWTETKKQSFKQTGEFGEKRKNSILNPINSIRKFSIVQKTPLQMNGIEEDSDEPLERRLSLVPDSEQGEAILPRISVISTGPTLQARRRQSVLNLMTHSVNQGQNIHRKTTASTRKVSLAPQANLTELDIYSRRLSQETGLEISEEINEEDLKECFFDDMESIPAVTTWNTYLRYITVHKSLIFVLIWCLVIFLAEVAASLVVLWLLGNTPLQDKGNSTHSRNNSYAVIITSTSSYYVFYIYVGVADTLLAMGFFRGLPLVHTLITVSKILHHKMLHSVLQAPMSTLNTLKAGGILNRFSKDIAILDDLLPLTIFDFIQLLLIVIGAIAVVAVLQPYIFVATVPVIVAFIMLRAYFLQTSQQLKQLESEGRSPIFTHLVTSLKGLWTLRAFGRQPYFETLFHKALNLHTANWFLYLSTLRWFQMRIEMIFVIFFIAVTFISILTTGEGEGRVGIILTLAMNIMSTLQWAVNSSIDVDSLMRSVSRVFKFIDMPTEGKPTKSTKPYKNGQLSKVMIIENSHVKKDDIWPSGGQMTVKDLTAKYTEGGNAILENISFSISPGQRVGLLGRTGSGKSTLLSAFLRLLNTEGEIQIDGVSWDSITLQQWRKAFGVIPQKVFIFSGTFRKNLDPYEQWSDQEIWKVADEVGLRSVIEQFPGKLDFVLVDGGCVLSHGHKQLMCLARSVLSKAKILLLDEPSAHLDPVTYQIIRRTLKQAFADCTVILCEHRIEAMLECQQFLVIEENKVRQYDSIQKLLNERSLFRQAISPSDRVKLFPHRNSSKCKSKPQIAALKEETEEEVQDTRL 8D082AA2E768C065 calu-cftr-1 2 5 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed partially purified partially purified P13569:p.Gly551Asp mutation mutation certain certain sequence position certain certain certain sequence position certain G D Tue Jun 30 18:46:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly551Asp' human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 physical association physical association fig. 2, Tables 1 & 2 cftr-calu-1 1 5 unspecified role unspecified role unspecified role unspecified role physiological level physiological level endogenous level endogenous endogenous endogenous level 6 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed P13569:p.Gly551Asp mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain G D Tue Jun 30 19:37:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly551Asp' physical association physical association fig. 3b calu-cftr-2 3 4 unspecified role unspecified role bait bait purified purified ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey purified purified direct interaction direct interaction 5a