UniProt UniProt http://www.uniprot.org Cooperativity of peptidoglycan synthases active in bacterial cell elongation. Cooperativity of peptidoglycan synthases active in bacterial cell elongation. Molecular microbiology 2012 imex curation Banzhaf M., van den Berg van Saparoea B., Terrak M., Fraipont C., Egan A., Philippe J., Zapun A., Breukink E., Nguyen-Distèche M., den Blaauwen T., Vollmer W., Banzhaf M., van den Berg van Saparoea B., Terrak M., Fraipont C., Egan A., Philippe J., Zapun A., Breukink E., Nguyen-Distèche M., den Blaauwen T., Vollmer W. I've not included the enzyme reaction as its a linked reaction involving a second,only partially described enzyme w.vollmer@ncl.ac.uk 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom western blot western blot Immuno blot Immuno blot Banzhaf M., van den Berg van Saparoea B., Terrak M., Fraipont C., Egan A., Philippe J., Zapun A., Breukink E., Nguyen-Distèche M., den Blaauwen T., Vollmer W., Banzhaf M., van den Berg van Saparoea B., Terrak M., Fraipont C., Egan A., Philippe J., Zapun A., Breukink E., Nguyen-Distèche M., den Blaauwen T., Vollmer W. I've not included the enzyme reaction as its a linked reaction involving a second,only partially described enzyme w.vollmer@ncl.ac.uk Accepted 2020-OCT-01 AT 12:59 BST AT 12:59 BST by MGT Molecular microbiology 2012 Only protein-protein interactions imex curation imex curation Cooperativity of peptidoglycan synthases active in bacterial cell elongation. Cooperativity of peptidoglycan synthases active in bacterial cell elongation. Molecular microbiology 2012 imex curation Banzhaf M., van den Berg van Saparoea B., Terrak M., Fraipont C., Egan A., Philippe J., Zapun A., Breukink E., Nguyen-Distèche M., den Blaauwen T., Vollmer W., Banzhaf M., van den Berg van Saparoea B., Terrak M., Fraipont C., Egan A., Philippe J., Zapun A., Breukink E., Nguyen-Distèche M., den Blaauwen T., Vollmer W. I've not included the enzyme reaction as its a linked reaction involving a second,only partially described enzyme w.vollmer@ncl.ac.uk 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting tag visualisation tag visualisation Banzhaf M., van den Berg van Saparoea B., Terrak M., Fraipont C., Egan A., Philippe J., Zapun A., Breukink E., Nguyen-Distèche M., den Blaauwen T., Vollmer W., Banzhaf M., van den Berg van Saparoea B., Terrak M., Fraipont C., Egan A., Philippe J., Zapun A., Breukink E., Nguyen-Distèche M., den Blaauwen T., Vollmer W. I've not included the enzyme reaction as its a linked reaction involving a second,only partially described enzyme w.vollmer@ncl.ac.uk Accepted 2020-OCT-01 AT 12:59 BST AT 12:59 BST by MGT Molecular microbiology 2012 Only protein-protein interactions imex curation imex curation Cooperativity of peptidoglycan synthases active in bacterial cell elongation. Cooperativity of peptidoglycan synthases active in bacterial cell elongation. Molecular microbiology 2012 imex curation Banzhaf M., van den Berg van Saparoea B., Terrak M., Fraipont C., Egan A., Philippe J., Zapun A., Breukink E., Nguyen-Distèche M., den Blaauwen T., Vollmer W., Banzhaf M., van den Berg van Saparoea B., Terrak M., Fraipont C., Egan A., Philippe J., Zapun A., Breukink E., Nguyen-Distèche M., den Blaauwen T., Vollmer W. I've not included the enzyme reaction as its a linked reaction involving a second,only partially described enzyme w.vollmer@ncl.ac.uk 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) adenylate cyclase adenylate cyclase complementation bacterial two-hybrid bacterial two-hybrid adenylate cyclase predetermined predetermined participant predetermined Banzhaf M., van den Berg van Saparoea B., Terrak M., Fraipont C., Egan A., Philippe J., Zapun A., Breukink E., Nguyen-Distèche M., den Blaauwen T., Vollmer W., Banzhaf M., van den Berg van Saparoea B., Terrak M., Fraipont C., Egan A., Philippe J., Zapun A., Breukink E., Nguyen-Distèche M., den Blaauwen T., Vollmer W. I've not included the enzyme reaction as its a linked reaction involving a second,only partially described enzyme w.vollmer@ncl.ac.uk Accepted 2020-OCT-01 AT 12:59 BST AT 12:59 BST by MGT Molecular microbiology 2012 Only protein-protein interactions imex curation imex curation Cooperativity of peptidoglycan synthases active in bacterial cell elongation. Cooperativity of peptidoglycan synthases active in bacterial cell elongation. Molecular microbiology 2012 imex curation Banzhaf M., van den Berg van Saparoea B., Terrak M., Fraipont C., Egan A., Philippe J., Zapun A., Breukink E., Nguyen-Distèche M., den Blaauwen T., Vollmer W., Banzhaf M., van den Berg van Saparoea B., Terrak M., Fraipont C., Egan A., Philippe J., Zapun A., Breukink E., Nguyen-Distèche M., den Blaauwen T., Vollmer W. I've not included the enzyme reaction as its a linked reaction involving a second,only partially described enzyme w.vollmer@ncl.ac.uk 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro fluorescence fluorescence technology fluorescence tag fluorescence tag visualisation by fluorescence tag fluorescence Banzhaf M., van den Berg van Saparoea B., Terrak M., Fraipont C., Egan A., Philippe J., Zapun A., Breukink E., Nguyen-Distèche M., den Blaauwen T., Vollmer W., Banzhaf M., van den Berg van Saparoea B., Terrak M., Fraipont C., Egan A., Philippe J., Zapun A., Breukink E., Nguyen-Distèche M., den Blaauwen T., Vollmer W. I've not included the enzyme reaction as its a linked reaction involving a second,only partially described enzyme w.vollmer@ncl.ac.uk Accepted 2020-OCT-01 AT 12:59 BST AT 12:59 BST by MGT Molecular microbiology 2012 Only protein-protein interactions imex curation imex curation Cooperativity of peptidoglycan synthases active in bacterial cell elongation. Cooperativity of peptidoglycan synthases active in bacterial cell elongation. Molecular microbiology 2012 imex curation Banzhaf M., van den Berg van Saparoea B., Terrak M., Fraipont C., Egan A., Philippe J., Zapun A., Breukink E., Nguyen-Distèche M., den Blaauwen T., Vollmer W., Banzhaf M., van den Berg van Saparoea B., Terrak M., Fraipont C., Egan A., Philippe J., Zapun A., Breukink E., Nguyen-Distèche M., den Blaauwen T., Vollmer W. I've not included the enzyme reaction as its a linked reaction involving a second,only partially described enzyme w.vollmer@ncl.ac.uk 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Banzhaf M., van den Berg van Saparoea B., Terrak M., Fraipont C., Egan A., Philippe J., Zapun A., Breukink E., Nguyen-Distèche M., den Blaauwen T., Vollmer W., Banzhaf M., van den Berg van Saparoea B., Terrak M., Fraipont C., Egan A., Philippe J., Zapun A., Breukink E., Nguyen-Distèche M., den Blaauwen T., Vollmer W. I've not included the enzyme reaction as its a linked reaction involving a second,only partially described enzyme Crosslinking with 3,3'-Dithiobis(sulfosuccinimidylpropionate) (DTSSP) followed by immunoprecipitation with anti‐PBP2. w.vollmer@ncl.ac.uk Accepted 2020-OCT-01 AT 12:59 BST AT 12:59 BST by MGT Molecular microbiology 2012 Only protein-protein interactions imex curation imex curation mecillinam mecillinam (2S,5R,6R)-6-[(azepan-1-ylmethylidene)amino]-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid amdinocillin Coactin mecilinamo mecillinam mecillinamum penicillin HX 6beta-{[(azepan-1-yl)methylidene]amino}-2,2-dimethylpenam-3alpha-carboxylic acid small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) penicillin penicillin Penicillin penicillins penicillins small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ampicillin ampicillin aminobenzylpenicillin AMP ampicilina (2S,5R,6R)-6-{[(2R)-2-amino-2-phenylacetyl]amino}-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid (2S,5R,6R)-6-{[(2R)-2-AMINO-2-PHENYLETHANOYL]AMINO}-3,3-DIMETHYL-7-OXO-4-THIA-1-AZABICYCLO[3.2.0]HEPTANE-2-CARBOXYLIC ACID (2S,6R)-6-{[(2R)-2-amino-2-phenylethanoyl]amino}-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid 6-(D-(2-amino-2-phenylacetamido))-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo(3.2.0)heptane-2-carboxylic acid ABPC Ampicillin ampicillin ampicillin acid ampicillin anhydrous ampicilline ampicillinum Anhydrous ampicillin AP D-(-)-6-(alpha-aminophenylacetamido)penicillanic acid D-(-)-ampicillin 6beta-[(2R)-2-amino-2-phenylacetamido]-2,2-dimethylpenam-3alpha-carboxylic acid small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) cefsulodin cefsulodin Cefsulodin cefsulodin cefsulodine cefsulodino cefsulodinum (6R,7R)-3-[(4-carbamoylpyridinium-1-yl)methyl]-8-oxo-7-{[(2R)-2-phenyl-2-sulfoacetyl]amino}-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate 7beta-{[(2R)-2-phenyl-2-sulfoacetyl]amino}-3-(4-carbamoylpyridinium-1-yl)methyl-3,4-didehydrocepham-4-carboxylate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) mrda_ecoli Peptidoglycan D,D-transpeptidase MrdA mrdA pbpA b0635 JW0630 Penicillin-binding protein 2 protein protein ecoli Escherichia coli (strain K12) MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQIVRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTLDALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKGYKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVLHGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVTDPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKPYVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFFYQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIPVGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIHSGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAERLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAENPAVAAAEDH FE2305C002743AF6 pbpa_ecoli Penicillin-binding protein 1A mrcA ponA b3396 JW3359 protein protein ecoli Escherichia coli (strain K12) MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRLQIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIFRAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEILELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRAVARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRYGESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNNKITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQGPTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQSKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDASAGSDWQPKNSPPQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSASFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDTQKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFLIKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVWIGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPGIVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF 0A54C6B28783E34B mrda-mrca-1 1 11 unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role bait bait purified purified ecoli Escherichia coli (strain K12) physical association physical association Fig. 1B mrca-mrda-1 1 11 unspecified role unspecified role bait bait purified purified ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level ecoli Escherichia coli (strain K12) physical association physical association Fig. 1B mrda-mrca-2 3 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed ecoli Escherichia coli (strain K12) physical association physical association Fig. 1C mrda-mrca-3 5 11 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level ecoli Escherichia coli (strain K12) physical association physical association Fig. 1D ampicillin-mrca-1 2 11 unspecified role unspecified role prey prey purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Fig. 1A mrca-mrda-2 4 7 unspecified role unspecified role neutral component neutral component region bodipy label bodipy label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) physical association physical association Fig. 6A cefsulodin-mrca-1 4 7 unspecified role unspecified role neutral component neutral component region bodipy label bodipy label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) physical association physical association Fig. 6A Cefsulodin specifically inhibits activity of PBP1A mecillinam-mrca-1 4 7 unspecified role unspecified role neutral component neutral component region bodipy label bodipy label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) physical association physical association Fig. 6A Mecillinam specifically inhibits activity of PBP2.