MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url&gt;http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it The rickettsia surface cell antigen 4 applies mimicry to bind to and activate vinculin. The rickettsia surface cell antigen 4 applies mimicry to bind to and activate vinculin. The Journal of biological chemistry 2011 imex curation Park H., Lee JH., Gouin E., Cossart P., Izard T. kernick@scripps.edu <kernick@scripps.edu>; 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Park H., Lee JH., Gouin E., Cossart P., Izard T. kernick@scripps.edu <kernick@scripps.edu>; Accepted 2020-OCT-13 AT 08:34 BST AT 08:34 BST by LIVIAP The Journal of biological chemistry 2011 Only protein-protein interactions imex curation imex curation The rickettsia surface cell antigen 4 applies mimicry to bind to and activate vinculin. The rickettsia surface cell antigen 4 applies mimicry to bind to and activate vinculin. The Journal of biological chemistry 2011 imex curation Park H., Lee JH., Gouin E., Cossart P., Izard T. kernick@scripps.edu <kernick@scripps.edu>; 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Park H., Lee JH., Gouin E., Cossart P., Izard T. kernick@scripps.edu <kernick@scripps.edu>; Accepted 2020-OCT-13 AT 08:34 BST AT 08:34 BST by LIVIAP The Journal of biological chemistry 2011 Only protein-protein interactions imex curation imex curation The rickettsia surface cell antigen 4 applies mimicry to bind to and activate vinculin. The rickettsia surface cell antigen 4 applies mimicry to bind to and activate vinculin. The Journal of biological chemistry 2011 imex curation Park H., Lee JH., Gouin E., Cossart P., Izard T. kernick@scripps.edu <kernick@scripps.edu>; 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight by staining molecular weight estimation by staining weight by staining Park H., Lee JH., Gouin E., Cossart P., Izard T. kernick@scripps.edu <kernick@scripps.edu>; Accepted 2020-OCT-13 AT 08:34 BST AT 08:34 BST by LIVIAP The Journal of biological chemistry 2011 Only protein-protein interactions imex curation imex curation The rickettsia surface cell antigen 4 applies mimicry to bind to and activate vinculin. The rickettsia surface cell antigen 4 applies mimicry to bind to and activate vinculin. The Journal of biological chemistry 2011 imex curation Park H., Lee JH., Gouin E., Cossart P., Izard T. kernick@scripps.edu <kernick@scripps.edu>; 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells confocal microscopy confocal microscopy immunostaining immunostaining Park H., Lee JH., Gouin E., Cossart P., Izard T. kernick@scripps.edu <kernick@scripps.edu>; Accepted 2020-OCT-13 AT 08:34 BST AT 08:34 BST by LIVIAP The Journal of biological chemistry 2011 Only protein-protein interactions imex curation imex curation vinc_human Vinculin Metavinculin VCL protein protein human Homo sapiens Human MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGSSPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGKLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGIPAAEVGIGVVAEADAADAAGFPVPPDMEDDYEPELLLMPSNQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ DIP protein P18206 original sequence version: 126 BFBD687DA836B0FA b0bxr4_ricro 120 kDa antigen Antigenic heat-stable 120 kDa protein Protein PS 120 RrIowa_0797 protein protein Rickettsia rickettsii (strain Iowa) Rickettsia rickettsii (strain Iowa) MRGFMSKDGNLDTSEFDPLANKEYTEEQKQTLEQEQKEFLSQTTTPELEADDGFIVTSESSAQSTPSMSALSGNISPDSQTSDPITKAVRETIIQPQKDNLIEQILKDLAALTDRDLAEQKRKEIEEEKEKDKTLSTFFGNPANREFIDKALEKPELKKKLESIEIAGYKNVHNTFSAASGYPGGFKPVQWENHVSASDLRATVVKNDAGDELCTLNETTVKTKPFTLAKQDGTQVQISSYREIDFPIKLDQADGSMHLSMVALKADGTKPSKDKAVYFTAHYEEGPNGKPQLKEISSPKPLKFAGTGDDAIAYIEHGGEIYTLAVTRGKYKEMMKEVELNQGQSVDLSQAEDIIIGQGQSKEQPLITPQQTTSSSVEPPQYKQQVPPITPTNQPLQPETSQMPQSQHVNPNLLNAATALSGSMQYLLNYVNAGLTKEIDSNQQIDLIKEAATVILNNEKSDIAEKQANIIAFAENTVNNKNLKPDAKVAGVNAVLETIKNDQNTPDLEKSKMLEATVAIVLNSENLEPKQKQQVLDKAVDVGLSLKDDASRAAAIDGIKDVVIKSNLSTVMLIAVGDKVNVSELSNAEKQKLLGSVLKKGVEAQVLSPAQQQLMQQHLDKIMAEQTKKDTIKKVNDILFDPLSNTELKTTNIQAIISNVLDGPATAVVKGEIIQEITNTVAGSSLEAQDKATIVKGVGETIATHSDTSLSLPNKALIMASAEKGIAESQTTLPDRELMTKGLVDGIYEGKGGPEITKAVSSGIDNSNINDSEKEALKKAKDAASETALDRDTQNLTEGLKGQNIEEHKPHDDIYNKAREVINAVNPVIEALEKSKEPVVSAEARIVQETSSILNNISKLAVEKVNNFRAMLSPNGNLKTLEEKKEESIKKVDELVKAFGTKSSTAEQQSFIKTNLIDDKTLSKEVRLQTIDKLLQEQKRAEAIENPSVKTEDVRVVSGKSQLKPISKDNPDIEKAKMVVGRDRVNIKGNIKIMGALMNARDIIQSENLNKSTPIKRESSPPQR 6635D0CCB1A31C1E vinc_mouse Vinculin Metavinculin Vcl protein protein mouse Mus musculus Mouse MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEIAALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKAQMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVISAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ 067F1B16A5285859 b0bxr4_ricro-vcl-3 3 6 unspecified role unspecified role neutral component neutral component purified purified binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role neutral component neutral component purified purified binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction fig.1b b0bxr4_ricro-vcl-5 3 6 unspecified role unspecified role unspecified role unspecified role purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined VBS-N binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain VBS-C binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction fig. 1c b0bxr4_ricro-vcl-4 3 6 competitor competitor competitor neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain 5 self self neutral component neutral component purified purified Vh1 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 5 self self neutral component neutral component purified purified Vt binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association fig. 2a Indeed, native gel analyses showed that the nearly full-length sca4 (residues 21–1008) as well as the domains harboring the VBSs (residues 411–585 and 774–1008) readily displaced the Vt tail domain from pre-existing Vh1Vt complexes by forming a new Vh1sca4-VBS complex (Fig. 2A). b0bxr4_ricro-vcl-6 4 7 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain colocalization colocalization fig. 3 Confocal microscopy established that eGFP-sca4, residues 406–585, 776–1008, or 21–1008 were present in fibrous-like structures and at punctate that overlapped with focal adhesions. Notably, both nearly full-length sca4s and the domains harboring the sca4-VBSs co-localized with endogenous vinculin. b0bxr4_ricro-vcl-2 1 5 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role neutral component neutral component purified purified VBS-N binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction table 1,2 b0bxr4_ricro-vcl-1 1 5 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role neutral component neutral component purified purified VBS-N binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction table 1,2 b0bxr4_ricro-vcl-7 2 5 unspecified role unspecified role neutral component neutral component purified purified Vh1 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction suppl. fig. 1,2