UniProt UniProt http://www.uniprot.org Intracellular trafficking of guanylate-binding proteins is regulated by heterodimerization in a hierarchical manner. Intracellular trafficking of guanylate-binding proteins is regulated by heterodimerization in a hierarchical manner. PloS one 2009 imex curation Britzen-Laurent N., Bauer M., Berton V., Fischer N., Syguda A., Reipschläger S., Naschberger E., Herrmann C., Stürzl M. michael.stuerzl@uk-erlangen.de 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Britzen-Laurent N., Bauer M., Berton V., Fischer N., Syguda A., Reipschläger S., Naschberger E., Herrmann C., Stürzl M. michael.stuerzl@uk-erlangen.de Accepted 2020-NOV-30 AT 10:08 GMT AT 10:08 GMT by MGT PloS one 2009 imex curation Intracellular trafficking of guanylate-binding proteins is regulated by heterodimerization in a hierarchical manner. Intracellular trafficking of guanylate-binding proteins is regulated by heterodimerization in a hierarchical manner. PloS one 2009 imex curation Britzen-Laurent N., Bauer M., Berton V., Fischer N., Syguda A., Reipschläger S., Naschberger E., Herrmann C., Stürzl M. michael.stuerzl@uk-erlangen.de 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Britzen-Laurent N., Bauer M., Berton V., Fischer N., Syguda A., Reipschläger S., Naschberger E., Herrmann C., Stürzl M. michael.stuerzl@uk-erlangen.de Accepted 2020-NOV-30 AT 10:08 GMT AT 10:08 GMT by MGT PloS one 2009 imex curation Intracellular trafficking of guanylate-binding proteins is regulated by heterodimerization in a hierarchical manner. Intracellular trafficking of guanylate-binding proteins is regulated by heterodimerization in a hierarchical manner. PloS one 2009 imex curation Britzen-Laurent N., Bauer M., Berton V., Fischer N., Syguda A., Reipschläger S., Naschberger E., Herrmann C., Stürzl M. michael.stuerzl@uk-erlangen.de 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Britzen-Laurent N., Bauer M., Berton V., Fischer N., Syguda A., Reipschläger S., Naschberger E., Herrmann C., Stürzl M. michael.stuerzl@uk-erlangen.de Accepted 2020-NOV-30 AT 10:08 GMT AT 10:08 GMT by MGT PloS one 2009 imex curation gbp5_human Guanylate-binding protein 5 Guanine nucleotide-binding protein 5 GTP-binding protein 5 GBP-TA antigen GBP5 UNQ2427/PRO4987 protein protein human Homo sapiens Human MALEIHMSDPMCLIENFNEQLKVNQEALEILSAITQPVVVVAIVGLYRTGKSYLMNKLAGKNKGFSVASTVQSHTKGIWIWCVPHPNWPNHTLVLLDTEGLGDVEKADNKNDIQIFALALLLSSTFVYNTVNKIDQGAIDLLHNVTELTDLLKARNSPDLDRVEDPADSASFFPDLVWTLRDFCLGLEIDGQLVTPDEYLENSLRPKQGSDQRVQNFNLPRLCIQKFFPKKKCFIFDLPAHQKKLAQLETLPDDELEPEFVQQVTEFCSYIFSHSMTKTLPGGIMVNGSRLKNLVLTYVNAISSGDLPCIENAVLALAQRENSAAVQKAIAHYDQQMGQKVQLPMETLQELLDLHRTSEREAIEVFMKNSFKDVDQSFQKELETLLDAKQNDICKRNLEASSDYCSALLKDIFGPLEEAVKQGIYSKPGGHNLFIQKTEELKAKYYREPRKGIQAEEVLQKYLKSKESVSHAILQTDQALTETEKKKKEAQVKAEAEKAEAQRLAAIQRQNEQMMQERERLHQEQVRQMEIAKQNWLAEQQKMQEQQMQEQAAQLSTTFQAQNRSLLSELQHAQRTVNNDDPCVLL 95DDC02F0FB705D0 gbp1_human Guanylate-binding protein 1 GBP1 GTP-binding protein 1 Guanine nucleotide-binding protein 1 Interferon-induced guanylate-binding protein 1 protein protein human Homo sapiens Human MASEIHMTGPMCLIENTNGRLMANPEALKILSAITQPMVVVAIVGLYRTGKSYLMNKLAGKKKGFSLGSTVQSHTKGIWMWCVPHPKKPGHILVLLDTEGLGDVEKGDNQNDSWIFALAVLLSSTFVYNSIGTINQQAMDQLYYVTELTHRIRSKSSPDENENEVEDSADFVSFFPDFVWTLRDFSLDLEADGQPLTPDEYLTYSLKLKKGTSQKDETFNLPRLCIRKFFPKKKCFVFDRPVHRRKLAQLEKLQDEELDPEFVQQVADFCSYIFSNSKTKTLSGGIQVNGPRLESLVLTYVNAISSGDLPCMENAVLALAQIENSAAVQKAIAHYEQQMGQKVQLPTETLQELLDLHRDSEREAIEVFIRSSFKDVDHLFQKELAAQLEKKRDDFCKQNQEASSDRCSALLQVIFSPLEEEVKAGIYSKPGGYRLFVQKLQDLKKKYYEEPRKGIQAEEILQTYLKSKESMTDAILQTDQTLTEKEKEIEVERVKAESAQASAKMLQEMQRKNEQMMEQKERSYQEHLKQLTEKMENDRVQLLKEQERTLALKLQEQEQLLKEGFQKESRIMKNEIQDLQTKMRRRKACTIS DIP protein P32455 original sequence version: 122 3A06741218360732 gbp2_human Guanylate-binding protein 2 Guanine nucleotide-binding protein 2 GTP-binding protein 2 GBP2 Interferon-induced guanylate-binding protein 2 protein protein human Homo sapiens Human MAPEINLPGPMSLIDNTKGQLVVNPEALKILSAITQPVVVVAIVGLYRTGKSYLMNKLAGKKNGFSLGSTVKSHTKGIWMWCVPHPKKPEHTLVLLDTEGLGDIEKGDNENDSWIFALAILLSSTFVYNSMGTINQQAMDQLHYVTELTDRIKANSSPGNNSVDDSADFVSFFPAFVWTLRDFTLELEVDGEPITADDYLELSLKLRKGTDKKSKSFNDPRLCIRKFFPKRKCFVFDWPAPKKYLAHLEQLKEEELNPDFIEQVAEFCSYILSHSNVKTLSGGIPVNGPRLESLVLTYVNAISSGDLPCMENAVLALAQIENSAAVEKAIAHYEQQMGQKVQLPTETLQELLDLHRDSEREAIEVFMKNSFKDVDQMFQRKLGAQLEARRDDFCKQNSKASSDCCMALLQDIFGPLEEDVKQGTFSKPGGYRLFTQKLQELKNKYYQVPRKGIQAKEVLKKYLESKEDVADALLQTDQSLSEKEKAIEVERIKAESAEAAKKMLEEIQKKNEEMMEQKEKSYQEHVKQLTEKMERDRAQLMAEQEKTLALKLQEQERLLKEGFENESKRLQKDIWDIQMRSKSLEPICNIL B09B3C2F3C3E1EA2 gbp3_human Guanylate-binding protein 3 GTP-binding protein 3 Guanine nucleotide-binding protein 3 GBP3 protein protein human Homo sapiens Human MAPEIHMTGPMCLIENTNGELVANPEALKILSAITQPVVVVAIVGLYRTGKSYLMNKLAGKNKGFSLGSTVKSHTKGIWMWCVPHPKKPEHTLVLLDTEGLGDVKKGDNQNDSWIFTLAVLLSSTLVYNSMGTINQQAMDQLYYVTELTHRIRSKSSPDENENEDSADFVSFFPDFVWTLRDFSLDLEADGQPLTPDEYLEYSLKLTQGTSQKDKNFNLPRLCIRKFFPKKKCFVFDLPIHRRKLAQLEKLQDEELDPEFVQQVADFCSYIFSNSKTKTLSGGIKVNGPRLESLVLTYINAISRGDLPCMENAVLALAQIENSAAVQKAIAHYDQQMGQKVQLPAETLQELLDLHRVSEREATEVYMKNSFKDVDHLFQKKLAAQLDKKRDDFCKQNQEASSDRCSALLQVIFSPLEEEVKAGIYSKPGGYCLFIQKLQDLEKKYYEEPRKGIQAEEILQTYLKSKESVTDAILQTDQILTEKEKEIEVECVKAESAQASAKMVEEMQIKYQQMMEEKEKSYQEHVKQLTEKMERERAQLLEEQEKTLTSKLQEQARVLKERCQGESTQLQNEIQKLQKTLKKKTKRYMSHKLKI 410A9BED9ABD2DDF gbp4_human Guanylate-binding protein 4 GTP-binding protein 4 Guanine nucleotide-binding protein 4 GBP4 protein protein human Homo sapiens Human MGERTLHAAVPTPGYPESESIMMAPICLVENQEEQLTVNSKALEILDKISQPVVVVAIVGLYRTGKSYLMNRLAGKRNGFPLGSTVQSETKGIWMWCVPHLSKPNHTLVLLDTEGLGDVEKSNPKNDSWIFALAVLLSSSFVYNSVSTINHQALEQLHYVTELAELIRAKSCPRPDEAEDSSEFASFFPDFIWTVRDFTLELKLDGNPITEDEYLENALKLIPGKNPKIQNSNMPRECIRHFFRKRKCFVFDRPTNDKQYLNHMDEVPEENLERHFLMQSDNFCSYIFTHAKTKTLREGIIVTGKRLGTLVVTYVDAINSGAVPCLENAVTALAQLENPAAVQRAADHYSQQMAQQLRLPTDTLQELLDVHAACEREAIAVFMEHSFKDENHEFQKKLVDTIEKKKGDFVLQNEEASAKYCQAELKRLSEHLTESILRGIFSVPGGHNLYLEEKKQVEWDYKLVPRKGVKANEVLQNFLQSQVVVEESILQSDKALTAGEKAIAAERAMKEAAEKEQELLREKQKEQQQMMEAQERSFQEYMAQMEKKLEEERENLLREHERLLKHKLKVQEEMLKEEFQKKSEQLNKEINQLKEKIESTKNEQLRLLKILDMASNIMIVTLPGASKLLGVGTKYLGSRI gbp1-gbp1-3 1 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 4A gbp1-gbp1-4 1 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Fig. 2A gbp2-gbp2-1 1 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 4A gbp2-gbp2-2 1 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Fig. 2A gbp5-gbp5-1 1 4 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 4A gbp5-gbp2-1 1 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Fig. 2A gbp1-gbp1-5 2 5 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region venus-c venus c-terminal fragment undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region venus-n venus n-terminal fragment undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 4B gbp2-gbp2-3 2 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 4B gbp5-gbp5-2 2 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 4B gbp1-gbp1-6 2 5 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P32455:p.Lys51Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K A 5 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Supp. Fig. 6 gbp2-gbp2-4 2 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 4C, Supp. Fig. 4 A/B When cells were treated with a farnesyl transferase inhibitor or geranylgeranyl transferase type I inhibitor, proteins could could still dimerize. However the fluorescent signals were evenly distributed throughout the cytoplasm indicating that isoprenylation is necessary for membrane affinity of the dimers. gbp5-gbp5-3 2 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 4C, Supp. Fig. 4B When cells were treated with a farnesyl transferase inhibitor or geranylgeranyl transferase type I inhibitor, proteins could could still dimerize. However the fluorescent signals were evenly distributed throughout the cytoplasm indicating that isoprenylation is necessary for membrane affinity of the dimers. gbp1-gbp2-2 1 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 5A gbp1-gbp5-1 1 4 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 5A gbp2-gbp1-1 1 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 5A gbp2-gbp5-1 1 4 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 5A gbp5-gbp1-1 1 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 5A gbp5-gbp2-2 1 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 5A gbp1-gbp2-3 1 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Fig. 2B gbp1-gbp5-2 1 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Fig. 2B gbp2-gbp1-2 1 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Fig. 2B gbp2-gbp5-2 1 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Fig. 2B gbp5-gbp1-2 1 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Fig. 2B gbp5-gbp2-3 1 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Fig. 2B gbp1-gbp2-4 2 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 5B gbp1-gbp2-5 2 5 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 5B, Supp. 7B Inhibition of GBP-1 farnesylation with a farnesyl transferase inhibitor induced localization to the perinuclear membranes. gbp1-gbp5-3 2 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range P32455:p.Lys51Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K A Sun Dec 13 19:35:06 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P32455:p.Lys51Ala[51-51]' physical association physical association Fig. 5B, Supp. Fig 5 gbp1-gbp5-4 2 5 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 5B, Supp. figure 7B Inhibition of GBP-1 farnesylation with a farnesyl transferase inhibitor induced localization to the perinuclear membranes. gbp2-gbp5-3 2 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 5B gbp1-gbp1-7 3 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association Supp. Fig. 3A gbp2-gbp2-5 3 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association Supp. Fig. 3A gbp5-gbp5-4 3 4 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association Supp. Fig. 3A gbp3-gbp3-1 3 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association Supp. Fig. 3A gbp4-gbp3-1 3 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association Supp. Fig. 3A gbp1-gbp1-8 2 5 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range P32455:p.Lys51Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K A physical association physical association 4C, Supp. Fig. 5 gbp1-gbp2-6 2 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed P32455:p.Lys51Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K A n venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Supp. Fig 5 gbp1-gbp5-5 2 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed P32455:p.Lys51Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K A n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Supp. Fig. 5 gbp1-gbp2-7 2 5 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P32455:p.Lys51Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K A 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 5B, Supp. fig 6 gbp1-gbp5-6 2 5 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P32455:p.Lys51Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K A 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Supp. Fig. 6 gbp2-gbp5-5 2 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 5B, Supp. Fig. 7B Treatment with GGTI (geranylgeranyl transferase type I inhibitor) resulted in a diffuse localization pattern for GBP-2/GBP-5 heterodimers . gbp1-gbp3-1 1 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Fig. 9A gbp1-gbp4-1 1 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Fig. 9A gbp2-gbp4-1 1 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Fig. 9A gbp2-gbp3-1 1 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Fig. 9A gbp3-gbp3-2 1 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Fig. 9A gbp4-gbp4-1 1 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Fig. 9A gbp5-gbp3-1 1 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. figure 9A gbp5-gbp4-1 1 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. figure 9A gbp1-gbp3-2 2 7 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 6 gbp1-gbp4-2 2 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 6 gbp2-gbp4-2 2 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 6 gbp2-gbp3-2 2 7 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 6 gbp3-gbp5-1 2 7 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 6 gbp4-gbp5-1 2 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 6 gbp2-gbp5-4 2 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Supp. Fig. 7A Treatment with GGTI (geranylgeranyl transferase type I inhibitor) resulted in a diffuse localization pattern for GBP-2/GBP-5 heterodimers .