UniProt UniProt http://www.uniprot.org Properties of the phage-shock-protein (Psp) regulatory complex that govern signal transduction and induction of the Psp response in Escherichia coli. Properties of the phage-shock-protein (Psp) regulatory complex that govern signal transduction and induction of the Psp response in Escherichia coli. Microbiology (Reading, England) 2010 imex curation Jovanovic G., Engl C., Mayhew AJ., Burrows PC., Buck M., Jovanovic G., Engl C., Mayhew AJ., Burrows PC., Buck M. Details of pspC LeuZipper mutation taken from strains and plasmids table in Supplementary materials. Details of pspB LeuZipper mutation taken from strains and plasmids table in Supplementary materials of PMID:19682256. m.buck@imperial.ac.uk 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) adenylate cyclase adenylate cyclase complementation bacterial two-hybrid bacterial two-hybrid adenylate cyclase predetermined predetermined participant predetermined Jovanovic G., Engl C., Mayhew AJ., Burrows PC., Buck M., Jovanovic G., Engl C., Mayhew AJ., Burrows PC., Buck M. As we don't add tags to BACTH expts, the tags aren't even in the CV, I've ignored the tag orientation, especially as it didn't seem to make any difference anyway. Details of pspC LeuZipper mutation taken from strains and plasmids table in Supplementary materials. Details of pspB LeuZipper mutation taken from strains and plasmids table in Supplementary materials of PMID:19682256. m.buck@imperial.ac.uk Accepted 2020-AUG-10 AT 15:00 BST AT 15:00 BST by MGT Microbiology (Reading, England) 2010 Only protein-protein interactions imex curation imex curation Properties of the phage-shock-protein (Psp) regulatory complex that govern signal transduction and induction of the Psp response in Escherichia coli. Properties of the phage-shock-protein (Psp) regulatory complex that govern signal transduction and induction of the Psp response in Escherichia coli. Microbiology (Reading, England) 2010 imex curation Jovanovic G., Engl C., Mayhew AJ., Burrows PC., Buck M., Jovanovic G., Engl C., Mayhew AJ., Burrows PC., Buck M. Details of pspC LeuZipper mutation taken from strains and plasmids table in Supplementary materials. Details of pspB LeuZipper mutation taken from strains and plasmids table in Supplementary materials of PMID:19682256. m.buck@imperial.ac.uk 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) pull down pull down western blot western blot Immuno blot Immuno blot Jovanovic G., Engl C., Mayhew AJ., Burrows PC., Buck M., Jovanovic G., Engl C., Mayhew AJ., Burrows PC., Buck M. Details of pspC LeuZipper mutation taken from strains and plasmids table in Supplementary materials. Details of pspB LeuZipper mutation taken from strains and plasmids table in Supplementary materials of PMID:19682256. m.buck@imperial.ac.uk Accepted 2020-AUG-10 AT 15:00 BST AT 15:00 BST by MGT Microbiology (Reading, England) 2010 Only protein-protein interactions imex curation imex curation Properties of the phage-shock-protein (Psp) regulatory complex that govern signal transduction and induction of the Psp response in Escherichia coli. Properties of the phage-shock-protein (Psp) regulatory complex that govern signal transduction and induction of the Psp response in Escherichia coli. Microbiology (Reading, England) 2010 imex curation Jovanovic G., Engl C., Mayhew AJ., Burrows PC., Buck M., Jovanovic G., Engl C., Mayhew AJ., Burrows PC., Buck M. Details of pspC LeuZipper mutation taken from strains and plasmids table in Supplementary materials. Details of pspB LeuZipper mutation taken from strains and plasmids table in Supplementary materials of PMID:19682256. m.buck@imperial.ac.uk 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) adenylate cyclase adenylate cyclase complementation bacterial two-hybrid bacterial two-hybrid adenylate cyclase predetermined predetermined participant predetermined Jovanovic G., Engl C., Mayhew AJ., Burrows PC., Buck M., Jovanovic G., Engl C., Mayhew AJ., Burrows PC., Buck M. Complementation assays performed in pspF-/-cells Details of pspC LeuZipper mutation taken from strains and plasmids table in Supplementary materials. Details of pspB LeuZipper mutation taken from strains and plasmids table in Supplementary materials of PMID:19682256. m.buck@imperial.ac.uk Accepted 2020-AUG-10 AT 15:00 BST AT 15:00 BST by MGT Microbiology (Reading, England) 2010 Only protein-protein interactions imex curation imex curation pspa_ecoli Phage shock protein A pspA b1304 JW1297 protein protein ecoli Escherichia coli (strain K12) MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVRSTSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIKSLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGKLDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMKQDNQ EAC29290406E5053 arcb_ecoli Aerobic respiration control sensor protein ArcB arcB b3210 JW5536 protein protein ecoli Escherichia coli (strain K12) MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTMVLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDLSLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSFLDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEKVFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDALERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNIFNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQVITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKIFAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSVAEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFKPGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDDVLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLITDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQSPDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK DIP protein P0AEC3 original sequence version: 45 DD61EA6ECF95AD30 pspb_ecoli Phage shock protein B JW1298 b1305 pspB protein protein ecoli Escherichia coli (strain K12) MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLAQLADEAKRMRERIQALESILDAEHPNWRDR 22B82DF367099A1B pspg_ecoli Phage shock protein G pspG yjbO b4050 JW5716 protein protein ecoli Escherichia coli (strain K12) MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALMIKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY 52F39814A605CCDD pspc_ecoli Phage shock protein C pspC b1306 JW1299 protein protein ecoli Escherichia coli (strain K12) MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLSIFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREMERYVTSDTFTLRSRFRQL DIP protein P0AFN2 original sequence version: 32 F63BED3EC5EDEE90 pspc-pspa-2 1 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 4 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association Table 1 pspa-pspa-1 1 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 4 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association Table 1 pspc-pspa-1 1 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 6 unspecified role unspecified role ancillary ancillary over-expressed over expressed level over-expressed physical association physical association Table 1 pspb-pspc-2 1 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed P0AFN2:p.Gly74Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G A Tue Jun 30 16:56:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly74Ala' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0AFN2:p.Gly48Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G A Tue Jun 30 16:58:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly48Ala' 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association Table 1 pspg-pspc-1 1 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association Table 1 pspa-pspc-3 1 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0AFN2:p.[Leu91Ala;Leu98Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A Tue Jun 30 17:13:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu91Ala;Leu98Ala]' 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 6 unspecified role unspecified role ancillary ancillary over-expressed over expressed level over-expressed physical association physical association Table 1 pspa-pspc-4 1 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed P0AFN2:p.Gly48Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G A Mon Jun 29 10:22:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'pGly48Ala' Tue Jun 30 18:13:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly48Ala' P0AFN2:p.Gly74Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G A Tue Jun 30 18:32:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly74Ala' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0AFN2:p.[Leu91Ala;Leu98Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A Tue Jun 30 16:45:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu91Ala;Leu98Ala]' region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association Table 1 pspa-arcb-1 1 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed P0AEC3:p.[Leu87Ala;Leu90Ala;Leu94Ala;Met97Ala] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain M A Mon Jun 29 10:26:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu87Ala;Leu90Ala;Leu94Ala;Leu97Ala]' Tue Jun 30 19:39:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu87Ala;Leu90Ala;Leu94Ala;Met97Ala]' 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association Table 1 pspc-arcb-1 1 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed P0AEC3:p.[Leu87Ala;Leu90Ala;Leu94Ala;Met97Ala] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain M A Mon Jun 29 10:24:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu87Ala;Leu90Ala;Leu94Ala;Leu97Ala]' Tue Jun 30 19:38:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu87Ala;Leu90Ala;Leu94Ala;Met97Ala]' 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association Table 1 pspb-arcb-1 1 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed P0AEC3:p.[Leu87Ala;Leu90Ala;Leu94Ala;Met97Ala] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain M A Mon Jun 29 09:58:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu87Ala;Leu90Ala;Leu94Ala;Leu97Ala]' Tue Jun 30 19:53:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu87Ala;Leu90Ala;Leu94Ala;Met97Ala]' 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association Table 1 pspb-pspb-2 1 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed P0AFM9:p.[Leu10Ala;Leu15Ala;Leu18Ala] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A Tue Jun 30 16:35:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu10Ala;Leu15Ala;Leu18Ala]' 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association Table 1 pspg-pspg-1 1 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association Table 1 pspc-pspb-3 1 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed P0AFM9:p.[Leu10Ala;Leu15Ala;Leu18Ala] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A physical association physical association Table 1 pspc-pspb-2 2 7 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 4 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed association association Fig2A-D pspc-pspg-1 2 7 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 2E Interaction observed in pspA-/- cells. pspa-pspa-2 3 4 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association Supp. Table 2 pspa-pspc-5 3 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0AFN2:p.[Leu91Ala;Leu98Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A Tue Jun 30 20:13:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu91Ala;Leu98Ala]' 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 6 unspecified role unspecified role ancillary ancillary over-expressed over expressed level over-expressed physical association physical association Supp. Table 2 pspa-pspc-1 3 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0AFN2:p.[Leu91Ala;Leu98Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A Tue Jun 30 17:11:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu91Ala;Leu98Ala]' 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association Supp. Table 2 Interaction only occurred with T18 in C-terminal (‘c’) protein fusion. pspc-pspa-3 3 4 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role ancillary ancillary over-expressed over expressed level over-expressed physical association physical association Supp. Table 2