IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Identification of Lynch syndrome mutations in the MLH1-PMS2 interface that disturb dimerization and mismatch repair. Identification of Lynch syndrome mutations in the MLH1-PMS2 interface that disturb dimerization and mismatch repair. Human mutation 2010 imex curation Kosinski J., Hinrichsen I., Bujnicki JM., Friedhoff P., Plotz G. Rare diseases - Interactions investigated in the context of Rare genetic disease plotz@med.uni-frankfurt.de 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kosinski J., Hinrichsen I., Bujnicki JM., Friedhoff P., Plotz G. Rare diseases - Interactions investigated in the context of Rare genetic disease pSG5-PMS2 construct gifted by Bert Vogelstein was inferred to be human (https://www.addgene.org/16475/) plotz@med.uni-frankfurt.de Accepted 2020-FEB-19 AT 10:47 GMT AT 10:47 GMT by MGT Human mutation 2010 Only protein-protein interactions imex curation imex curation mlh1_human DNA mismatch repair protein Mlh1 MutL protein homolog 1 MLH1 COCA2 protein protein human Homo sapiens Human MSFVAGVIRRLDETVVNRIAAGEVIQRPANAIKEMIENCLDAKSTSIQVIVKEGGLKLIQIQDNGTGIRKEDLDIVCERFTTSKLQSFEDLASISTYGFRGEALASISHVAHVTITTKTADGKCAYRASYSDGKLKAPPKPCAGNQGTQITVEDLFYNIATRRKALKNPSEEYGKILEVVGRYSVHNAGISFSVKKQGETVADVRTLPNASTVDNIRSIFGNAVSRELIEIGCEDKTLAFKMNGYISNANYSVKKCIFLLFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEESILERVQQHIESKLLGSNSSRMYFTQTLLPGLAGPSGEMVKSTTSLTSSSTSGSSDKVYAHQMVRTDSREQKLDAFLQPLSKPLSSQPQAIVTEDKTDISSGRARQQDEEMLELPAPAEVAAKNQSLEGDTTKGTSEMSEKRGPTSSNPRKRHREDSDVEMVEDDSRKEMTAACTPRRRIINLTSVLSLQEEINEQGHEVLREMLHNHSFVGCVNPQWALAQHQTKLYLLNTTKLSEELFYQILIYDFANFGVLRLSEPAPLFDLAMLALDSPESGWTEEDGPKEGLAEYIVEFLKKKAEMLADYFSLEIDEEGNLIGLPLLIDNYVPPLEGLPIFILRLATEVNWDEEKECFESLSKECAMFYSIRKQYISEESTLSGQQSEVPGSIPNSWKWTVEHIVYKALRSHILPPKHFTEDGNILQLANLPDLYKVFERC DIP protein P40692 original sequence version: 99 C9231FC406C2CA20 pms2_human Mismatch repair endonuclease PMS2 PMS1 protein homolog 2 DNA mismatch repair protein PMS2 PMS2 PMSL2 protein protein human Homo sapiens Human MERAESSSTEPAKAIKPIDRKSVHQICSGQVVLSLSTAVKELVENSLDAGATNIDLKLKDYGVDLIEVSDNGCGVEEENFEGLTLKHHTSKIQEFADLTQVETFGFRGEALSSLCALSDVTISTCHASAKVGTRLMFDHNGKIIQKTPYPRPRGTTVSVQQLFSTLPVRHKEFQRNIKKEYAKMVQVLHAYCIISAGIRVSCTNQLGQGKRQPVVCTGGSPSIKENIGSVFGQKQLQSLIPFVQLPPSDSVCEEYGLSCSDALHNLFYISGFISQCTHGVGRSSTDRQFFFINRRPCDPAKVCRLVNEVYHMYNRHQYPFVVLNISVDSECVDINVTPDKRQILLQEEKLLLAVLKTSLIGMFDSDVNKLNVSQQPLLDVEGNLIKMHAADLEKPMVEKQDQSPSLRTGEEKKDVSISRLREAFSLRHTTENKPHSPKTPEPRRSPLGQKRGMLSSSTSGAISDKGVLRPQKEAVSSSHGPSDPTDRAEVEKDSGHGSTSVDSEGFSIPDTGSHCSSEYAASSPGDRGSQEHVDSQEKAPKTDDSFSDVDCHSNQEDTGCKFRVLPQPTNLATPNTKRFKKEEILSSSDICQKLVNTQDMSASQVDVAVKINKKVVPLDFSMSSLAKRIKQLHHEAQQSEGEQNYRKFRAKICPGENQAAEDELRKEISKTMFAEMEIIGQFNLGFIITKLNEDIFIVDQHATDEKYNFEMLQQHTVLQGQRLIAPQTLNLTAVNEAVLIENLEIFRKNGFDFVIDENAPVTERAKLISLPTSKNWTFGPQDVDELIFMLSDSPGVMCRPSRVKQMFASRACRKSVMIGTALNTSEMKKLITHMGEMDHPWNCPHGRPTMRHIANLGVISQN B1B9547280ECAF9A mlh1-pms2-9 1 2 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed P40692:p.Gln542Leu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Q L Tue Jun 30 17:05:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln542Leu' P40692:p.Leu622His mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L H Tue Jun 30 17:22:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu622His' P40692:p.Leu636Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L P Tue Jun 30 18:01:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu636Pro' P40692:p.Arg659Gln mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain R Q Tue Jun 30 19:06:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg659Gln' P40692:p.Thr662Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T P Tue Jun 30 20:04:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr662Pro' P40692:p.Glu663Gly mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E G Tue Jun 30 20:07:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu663Gly' P40692:p.Leu749Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L P Tue Jun 30 19:52:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu749Pro' p.Tyr750* p.Tyr750* mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain YKVFERC . P40692:p.Asn551Thr mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N T Tue Jun 30 19:27:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn551Thr' P40692:p.Leu559Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L R Tue Jun 30 15:57:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu559Arg' P40692:p.Ala586Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A P Tue Jun 30 18:41:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala586Pro' P40692:p.Asp601Gly mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain D G Tue Jun 30 16:52:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp601Gly' P40692:p.Lys618Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 19:25:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys618Ala' P40692:p.Pro648Leu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain P L Tue Jun 30 19:18:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro648Leu' P40692:p.Pro654Leu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain P L Tue Jun 30 16:16:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro654Leu' P40692:p.Arg659Leu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R L Tue Jun 30 19:06:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg659Leu' P40692:p.Arg755Ser mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain R S Tue Jun 30 17:13:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg755Ser' P40692:p.Arg755Trp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R W Tue Jun 30 17:08:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg755Trp' 3 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association Figure 3