IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5. Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5. Archives of biochemistry and biophysics 2006 imex curation Cortesio CL., Jiang W. weipingj@rndsystems.com Only protein-protein interactions imex curation in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Cortesio CL., Jiang W. Amidolytic activity using a fluorometric assay based on the measurement of AMC (aminomethylcoumarin) released upon cleavage. Activity was measured in units -pmol/min/ug enzyme. Peptide sequences are given in Table 1. weipingj@rndsystems.com Accepted 2021-DEC-16 AT 15:20 GMT AT 15:20 GMT by SIMONA Archives of biochemistry and biophysics 2006 Only protein-protein interactions imex curation imex curation Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5. Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5. Archives of biochemistry and biophysics 2006 imex curation Cortesio CL., Jiang W. weipingj@rndsystems.com Only protein-protein interactions imex curation in vitro In vitro protease assay protease assay experimental particp experimental participant identification experimental particp Cortesio CL., Jiang W. N-terminal sequencing has been assumed to have been carried out by Edman degradation as no other method was found when searching for this term and "Molecular Structure Facility, University of California, Davis". IGFBP-5 is of Human origin as deduced by specific residues given- notably sequence TAHPRIISAP. weipingj@rndsystems.com Accepted 2021-DEC-16 AT 15:20 GMT AT 15:20 GMT by SIMONA Archives of biochemistry and biophysics 2006 Only protein-protein interactions imex curation imex curation p48740-2 Mannan-binding lectin serine protease 1 Mannose-binding protein-associated serine protease Ra-reactive factor serine protease p100 Serine protease 5 MASP-3 MASP1 CRARF CRARF1 PRSS5 Complement factor MASP-3 Complement-activating component of Ra-reactive factor Mannose-binding lectin-associated serine protease 1 protein protein human Homo sapiens Human MRWLLLYYALCFSLSKASAHTVELNNMFGQIQSPGYPDSYPSDSEVTWNITVPDGFRIKLYFMHFNLESSYLCEYDYVKVETEDQVLATFCGRETTDTEQTPGQEVVLSPGSFMSITFRSDFSNEERFTGFDAHYMAVDVDECKEREDEELSCDHYCHNYIGGYYCSCRFGYILHTDNRTCRVECSDNLFTQRTGVITSPDFPNPYPKSSECLYTIELEEGFMVNLQFEDIFDIEDHPEVPCPYDYIKIKVGPKVLGPFCGEKAPEPISTQSHSVLILFHSDNSGENRGWRLSYRAAGNECPELQPPVHGKIEPSQAKYFFKDQVLVSCDTGYKVLKDNVEMDTFQIECLKDGTWSNKIPTCKIVDCRAPGELEHGLITFSTRNNLTTYKSEIKYSCQEPYYKMLNNNTGIYTCSAQGVWMNKVLGRSLPTCLPECGQPSRSLPSLVKRIIGGRNAEPGLFPWQALIVVEDTSRVPNDKWFGSGALLSASWILTAAHVLRSQRRDTTVIPVSKEHVTVYLGLHDVRDKSGAVNSSAARVVLHPDFNIQNYNHDIALVQLQEPVPLGPHVMPVCLPRLEPEGPAPHMLGLVAGWGISNPNVTVDEIISSGTRTLSDVLQYVKLPVVPHAECKTSYESRSGNYSVTENMFCAGYYEGGKDTCLGDSGGAFVIFDDLSQRWVVQGLVSWGGPEECGSKQVYGVYTKVSNYVDWVWEQMGLPQSVVEPQVER 09B5297A6C14283A p00974-pro_0000016853 Pancreatic trypsin inhibitor Aprotinin Basic protease inhibitor protein protein bovin Bos taurus (Bovine) Bovine RPDFCLEPPYTGPCKARIIRYFYNAKAGLCQTFVYGGCRAKRNNFKSAEDCMRTCGGA 36 93 BD1DF874D6EA1518 leu-arg peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) LR ethylenediaminetetraacetic acid ethylenediaminetetraacetic acid (ethylenedinitrilo)tetraacetic acid, ion(4-) 2,2',2'',2'''-(ethane-1,2-diyldinitrilo)tetraacetate acide edetique Acide ethylenediaminetetracetique acido edetico acidum edeticum Edetic acid edetic acid EDTA edta EDTA, ion(4-) ethylenediaminetetraacetate ethylenediaminetetraacetic acid Ethylenediaminetetraacetic acid H4edta N,N'-1,2-Ethane diylbis-(N-(carboxymethyl)glycine) {[-(BIS-CARBOXYMETHYL-AMINO)-ETHYL]-CARBOXYMETHYL-AMINO}-ACETIC ACID (ethane-1,2-diyldinitrilo)tetraacetate (ethane-1,2-diyldinitrilo)tetraacetic acid small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) p03973-pro_0000041355 Antileukoproteinase SLPI WAP4 WFDC4 BLPI HUSI-1 Mucus proteinase inhibitor Protease inhibitor WAP4 Secretory leukocyte protease inhibitor Seminal proteinase inhibitor WAP four-disulfide core domain protein 4 protein protein human Homo sapiens Human SGKSFKAGVCPPKKSAQCLRYKKPECQSDWQCPGKKRCCPDTCGIKCLDPVDTPNPTRRKPGKCPVTYGQCLMLNPPNFCEMDGQCKRDLKCCMGMCGKSCVSPVKA 26 132 4C356A3AD419F292 aebsf 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) 3,4-dichloroisocoumarin 3,4-dichloroisocoumarin 3,4-DCI 3,4-dichloro-1H-2-benzopyran-1-one 3,4-dichloro-2-benzopyran-1-one 3,4-dichloroisochromen-1-one 3,4-dichloro-1H-isochromen-1-one small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) p23827-pro_0000007423 Ecotin eco eti b2209 JW2197 protein protein ecoli Escherichia coli (strain K12) AESVQPLEKIAPYPQAEKGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAVVR 21 162 2F4ECAA8B9138368 ipsp_human Plasma serine protease inhibitor Serpin A5 Protein C inhibitor Plasminogen activator inhibitor 3 Acrosomal serine protease inhibitor SERPINA5 PCI PLANH3 PROCI protein protein human Homo sapiens Human MQLFLLLCLVLLSPQGASLHRHHPREMKKRVEDLHVGATVAPSSRRDFTFDLYRALASAAPSQSIFFSPVSISMSLAMLSLGAGSSTKMQILEGLGLNLQKSSEKELHRGFQQLLQELNQPRDGFQLSLGNALFTDLVVDLQDTFVSAMKTLYLADTFPTNFRDSAGAMKQINDYVAKQTKGKIVDLLKNLDSNAVVIMVNYIFFKAKWETSFNHKGTQEQDFYVTSETVVRVPMMSREDQYHYLLDRNLSCRVVGVPYQGNATALFILPSEGKMQQVENGLSEKTLRKWLKMFKKRQLELYLPKFSIEGSYQLEKVLPSLGISNVFTSHADLSGISNHSNIQVSEMVHKAVVEVDESGTRAAAATGTIFTFRSARLNSQRLVFNRPFLMFIVDNNILFLGKVNRP DIP protein P05154 original sequence version: 108 2A8FF3DC33C77E04 gly-gly-arg peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) GGR pro-phe-arg peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) PFR ibp5_human Insulin-like growth factor-binding protein 5 IGFBP5 IBP5 protein protein human Homo sapiens Human MVLLTAVLLLLAAYAGPAQSLGSFVHCEPCDEKALSMCPPSPLGCELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYREQVKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRIISAPEMRQESEQGPCRRHMEASLQELKASPRMVPRAVYLPNCDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHTFDSSNVE DIP protein P24593 original sequence version: 117 0A7AD37C6EEA3A81 phe-arg peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) FR e64 E64 (2S,3S)-3-(N-{(S)-1-[N-(4-guanidinobutyl)carbamoyl]3-methylbutyl}carbamoyl)oxirane-2-carboxylic acid (2S,3S)-3-[((1S)-1-{[4-(carbamimidamido)butyl]carbamoyl}-3-methylbutyl)carbamoyl]oxirane-2-carboxylic acid E 64 E-64 small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) val-pro-arg peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) VPR masp1-pfr-2 1 3 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc 13 enzyme target enzyme target substrate substrate neutral component neutral component C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage Table 1 Activity=11.0 pmoles/min/ug masp1-vpr-2 1 3 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc 17 enzyme target enzyme target substrate substrate neutral component neutral component C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range t-Butyloxycarbonyl modval modified L-valine residue ModVal certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage Table 1 Activity = 9.0 pmoles/min/ug ggr-masp1-2 1 3 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc 12 enzyme target enzyme target substrate substrate neutral component neutral component C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range Benzyloxycarbonyl modgly modified glycine residue ModGly certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage Table 1 Activity = 5.6 pmoles/min/ug masp1-phe_arg-1 1 3 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc 15 enzyme target enzyme target substrate substrate neutral component neutral component C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range Benzyloxycarbonyl modphe modified L-phenylalanine residue ModPhe certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage Table 1 Activity = 1.5 pmoles/min/ug lr-masp1-1 1 3 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc 5 enzyme target enzyme target substrate substrate neutral component neutral component C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range Benzyloxycarbonyl modleu modified L-leucine residue ModLeu certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage Table 1 Activity = 1.2 pmoles/min/ug eco-masp1-5 1 10 inhibitor inhibitor unspecified role unspecified role purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 3 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc 17 enzyme target enzyme target substrate substrate neutral component neutral component C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range t-Butyloxycarbonyl modval modified L-valine residue ModVal certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage Table 2 Ecotin % inhibition = 98. masp1-serpina5-1 1 11 inhibitor inhibitor unspecified role unspecified role purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mouse-nso NSO-mouse myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc 3 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc 17 enzyme target enzyme target substrate substrate neutral component neutral component C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range t-Butyloxycarbonyl modval modified L-valine residue ModVal certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage Table 2 SerpinA5 % inhibition = 30. masp1-pro_0000016853-1 1 4 inhibitor inhibitor unspecified role unspecified role purified purified bovin-lung Bovin lung -Cow lung lung 3 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc 17 enzyme target enzyme target substrate substrate neutral component neutral component C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range t-Butyloxycarbonyl modval modified L-valine residue ModVal certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage Table 2 Aprotinin % inhibition = 8. masp1-pro_0000016853-2 1 7 inhibitor inhibitor unspecified role unspecified role purified purified ecoli Escherichia coli (strain K12) 3 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc 17 enzyme target enzyme target substrate substrate neutral component neutral component C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range t-Butyloxycarbonyl modval modified L-valine residue ModVal certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage Table 2 SLP1 % inhibition = 13. aebsf-masp1-1 1 8 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 3 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc 17 enzyme target enzyme target substrate substrate neutral component neutral component C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range t-Butyloxycarbonyl modval modified L-valine residue ModVal certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage Table 2 AEBSF % inhibition = 98. 3,4_dichloroisocoumarin-masp1-1 1 9 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 3 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc 17 enzyme target enzyme target substrate substrate neutral component neutral component C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range t-Butyloxycarbonyl modval modified L-valine residue ModVal certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage Table 2 AEBSF % inhibition = 100. e64-masp1-1 1 16 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 3 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc 17 enzyme target enzyme target substrate substrate neutral component neutral component C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range t-Butyloxycarbonyl modval modified L-valine residue ModVal certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage Table 2 E-64 % inhibition = 17. ethylenediaminetetraacetic acid-masp1-1 1 6 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 3 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc 17 enzyme target enzyme target substrate substrate neutral component neutral component C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range t-Butyloxycarbonyl modval modified L-valine residue ModVal certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage Table 2 E-64 % inhibition = 21. igfbp5-masp1-1 2 3 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc 14 weight by staining molecular weight estimation by staining weight by staining edman degradation edman degradation experimental particp experimental participant identification experimental particp enzyme target enzyme target substrate substrate neutral component neutral component arg176-ile177 biological feature biological feature certain certain sequence position certain certain certain sequence position certain resulting-cleavage resulting-cleavage lys140-his141 biological feature biological feature certain certain sequence position certain certain certain sequence position certain resulting-cleavage resulting-cleavage lys135-ile136 biological feature biological feature certain certain sequence position certain certain certain sequence position certain resulting-cleavage resulting-cleavage protein cleavage protein cleavage Figure 2, 3, 4.