IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature 2003 imex curation Li W., Moore MJ., Vasilieva N., Sui J., Wong SK., Berne MA., Somasundaran M., Sullivan JL., Luzuriaga K., Greenough TC., Choe H., Farzan M. Coronavirus - Interactions investigated in the context of Coronavirus hyeryun.choe@tch.harvard.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip weight by staining molecular weight estimation by staining weight by staining Li W., Moore MJ., Vasilieva N., Sui J., Wong SK., Berne MA., Somasundaran M., Sullivan JL., Luzuriaga K., Greenough TC., Choe H., Farzan M. Coronavirus - Interactions investigated in the context of Coronavirus hyeryun.choe@tch.harvard.edu Accepted 2020-MAR-26 AT 09:23 GMT AT 09:23 GMT by ORCHARD Nature 2003 Only protein-protein interactions imex curation imex curation Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature 2003 imex curation Li W., Moore MJ., Vasilieva N., Sui J., Wong SK., Berne MA., Somasundaran M., Sullivan JL., Luzuriaga K., Greenough TC., Choe H., Farzan M. Coronavirus - Interactions investigated in the context of Coronavirus hyeryun.choe@tch.harvard.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-vero Chlorocebus (Cercopithecus) aethiops kidney cells Grivet vero Chlorocebus (Cercopithecus) aethiops kidney cells similar to cos-7 cells but these are not trasnformed anti bait coip anti bait coimmunoprecipitation anti bait coip ms participant Identification by mass spectrometry ms participant Li W., Moore MJ., Vasilieva N., Sui J., Wong SK., Berne MA., Somasundaran M., Sullivan JL., Luzuriaga K., Greenough TC., Choe H., Farzan M. Coronavirus - Interactions investigated in the context of Coronavirus hyeryun.choe@tch.harvard.edu Accepted 2020-MAR-26 AT 09:23 GMT AT 09:23 GMT by ORCHARD Nature 2003 Only protein-protein interactions imex curation imex curation q1lzx8_chlae Angiotensin-converting enzyme ACE2 protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGEKWSAFLKEQSTLAQMYPLQAIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIHSTGKVCNPNNPQECLLLDPGLNEIMEKSLDYNERLWAWEGWRSEVGKQLRPLYEEYVVLKNEMARANHYKDYGDYWRGDYEVNGVDGYDYNRDQLIEDVERTFEEIKPLYEHLHAYVRAKLMNAYPSYISPTGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVNQAWNAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKVVCHPTAWDLGKGDFRIIMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLLNMLKLGKSEPWTLALENVVGAKNMSVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGANAYKWNDNEMYLFRSSVAYAMRQYFLENKHQTILFGEEDVRVADLKPRISFNFYVTAPKNVSDIIPRTEVEEAIRFSRSRINDAFQLNDNSLEFLGIQSTLVPPYQSPITTWLIVFGVVMAVIVAGIVVLIFTGIRDRKKKNQARSEENPYASIDISKGENNPGFQNTDDVQTSF CF37FF7C7E2C2E2F p59594-pro_0000037209 Spike protein S1 E2 Peplomer protein 2 S protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus SDLDRCTTFDDVQAPNYTQHTSSMRGVYYPDEIFRSDTLYLTQDLFLPFYSNVTGFHTINHTFGNPVIPFKDGIYFAATEKSNVVRGWVFGSTMNNKSQSVIIINNSTNVVIRACNFELCDNPFFAVSKPMGTQTHTMIFDNAFNCTFEYISDAFSLDVSEKSGNFKHLREFVFKNKDGFLYVYKGYQPIDVVRDLPSGFNTLKPIFKLPLGINITNFRAILTAFSPAQDIWGTSAAAYFVGYLKPTTFMLKYDENGTITDAVDCSQNPLAELKCSVKSFEIDKGIYQTSNFRVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNFNFNGLTGTGVLTPSSKRFQPFQQFGRDVSDFTDSVRDPKTSEILDISPCSFGGVSVITPGTNASSEVAVLYQDVNCTDVSTAIHADQLTPAWRIYSTGNNVFQTQAGCLIGAEHVDTSYECDIPIGAGICASYHTVSLLR 14 667 2A0998E14669E24D myo1b_chlae_protein Green monkey Unconventional myosin-Ib (MYH-1c) (Myosin I alpha, MMI-alpha, MMIa) protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey similar to human O43795 UniProt entry ace2_human Angiotensin-converting enzyme 2 ACE2 UNQ868/PRO1885 Angiotensin-converting enzyme homolog Metalloprotease MPROT15 Angiotensin-converting enzyme-related carboxypeptidase protein protein human Homo sapiens Human MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSIWLIVFGVVMGVIVVGIVILIFTGIRDRKKKNKARSGENPYASIDISKGENNPGFQNTDDVQTSF mint Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses 8EE6EB0A931550E8 mvp_chlae_protein Green monkey Major vault protein, MVP (Lung resistance-related protein) protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey similar to human Q14764 UniProt entry ace2-s1-1 2 7 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 5 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 3 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association Fig. 1C, Table S1 s-ace2-3 1 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed tag fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 2C Prey was estimated to be ACE2 by weight by comparison to the over-expressed protein identified by western blot.