IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ High-affinity multivalent interactions between apolipoprotein E and the oligomers of amyloid-β. High-affinity multivalent interactions between apolipoprotein E and the oligomers of amyloid-β. The FEBS journal 2019 imex curation Ghosh S., Sil TB., Dolai S., Garai K. Alzheimers - Interactions investigated in the context of Alzheimers disease email sent to authors 13/12/ regarding tags/method for fig 8A/B. ( no reply by 30/12/19 so sent for checking ) "Regarding Figure 8 A/B; Experiments are stated to be using TMR-Ab42 and monitored by ThT fluorescence, in the image text - under the image. However the images themselves do NOT mention TMR, and as TMR tagged Ab42 is stated in the Methods section to be monitored by a decrease in TMR fluorescence, it has been assumed that Ab42 is NOT tagged and monitored by ThT fluorescence, and not a decrease in TMR fluorescence. Is this correct? " Abeta-42 is of Human origin based on the sequence given on aapptec web-site and APOE is of Human origin based on mutation mapping. Source of recombinant APOE traced to pmid:25207746 kanchan@tifrh.res.in Only protein-protein interactions imex curation in vitro In vitro atomic force microsc atomic force microscopy AFM AFM atomic force microsc predetermined predetermined participant predetermined Ghosh S., Sil TB., Dolai S., Garai K. Alzheimers - Interactions investigated in the context of Alzheimers disease email sent to authors 13/12/ regarding tags/method for fig 8A/B. ( no reply by 30/12/19 so sent for checking ) "Regarding Figure 8 A/B; Experiments are stated to be using TMR-Ab42 and monitored by ThT fluorescence, in the image text - under the image. However the images themselves do NOT mention TMR, and as TMR tagged Ab42 is stated in the Methods section to be monitored by a decrease in TMR fluorescence, it has been assumed that Ab42 is NOT tagged and monitored by ThT fluorescence, and not a decrease in TMR fluorescence. Is this correct? " Abeta-42 is of Human origin based on the sequence given on aapptec web-site and APOE is of Human origin based on mutation mapping. Source of recombinant APOE traced to pmid:25207746 kanchan@tifrh.res.in Accepted 2020-MAY-08 AT 05:26 BST AT 05:26 BST by KALPANAP The FEBS journal 2019 Only protein-protein interactions imex curation imex curation High-affinity multivalent interactions between apolipoprotein E and the oligomers of amyloid-β. High-affinity multivalent interactions between apolipoprotein E and the oligomers of amyloid-β. The FEBS journal 2019 imex curation Ghosh S., Sil TB., Dolai S., Garai K. Alzheimers - Interactions investigated in the context of Alzheimers disease email sent to authors 13/12/ regarding tags/method for fig 8A/B. ( no reply by 30/12/19 so sent for checking ) "Regarding Figure 8 A/B; Experiments are stated to be using TMR-Ab42 and monitored by ThT fluorescence, in the image text - under the image. However the images themselves do NOT mention TMR, and as TMR tagged Ab42 is stated in the Methods section to be monitored by a decrease in TMR fluorescence, it has been assumed that Ab42 is NOT tagged and monitored by ThT fluorescence, and not a decrease in TMR fluorescence. Is this correct? " Abeta-42 is of Human origin based on the sequence given on aapptec web-site and APOE is of Human origin based on mutation mapping. Source of recombinant APOE traced to pmid:25207746 kanchan@tifrh.res.in Only protein-protein interactions imex curation in vitro In vitro aggregation aggregation assay aggregation predetermined predetermined participant predetermined Ghosh S., Sil TB., Dolai S., Garai K. Alzheimers - Interactions investigated in the context of Alzheimers disease Figure 8; Experiments are stated to be using TMR-Ab42 in the image text, and monitored by ThT fluorescence. The image itself does not mention TMR and as TMR tagged Ab42 is monitored by a decrease in TMR fluorescence it has been assumed that Ab42 is NOT tagged. email sent to authors 13/12/ regarding tags/method for fig 8A/B. ( no reply by 30/12/19 so sent for checking ) "Regarding Figure 8 A/B; Experiments are stated to be using TMR-Ab42 and monitored by ThT fluorescence, in the image text - under the image. However the images themselves do NOT mention TMR, and as TMR tagged Ab42 is stated in the Methods section to be monitored by a decrease in TMR fluorescence, it has been assumed that Ab42 is NOT tagged and monitored by ThT fluorescence, and not a decrease in TMR fluorescence. Is this correct? " Aggregation is monitored by quenching of TMR fluorescence. Abeta-42 is of Human origin based on the sequence given on aapptec web-site and APOE is of Human origin based on mutation mapping. Source of recombinant APOE traced to pmid:25207746 kanchan@tifrh.res.in Accepted 2020-MAY-08 AT 05:26 BST AT 05:26 BST by KALPANAP The FEBS journal 2019 Only protein-protein interactions imex curation imex curation High-affinity multivalent interactions between apolipoprotein E and the oligomers of amyloid-β. High-affinity multivalent interactions between apolipoprotein E and the oligomers of amyloid-β. The FEBS journal 2019 imex curation Ghosh S., Sil TB., Dolai S., Garai K. Alzheimers - Interactions investigated in the context of Alzheimers disease email sent to authors 13/12/ regarding tags/method for fig 8A/B. ( no reply by 30/12/19 so sent for checking ) "Regarding Figure 8 A/B; Experiments are stated to be using TMR-Ab42 and monitored by ThT fluorescence, in the image text - under the image. However the images themselves do NOT mention TMR, and as TMR tagged Ab42 is stated in the Methods section to be monitored by a decrease in TMR fluorescence, it has been assumed that Ab42 is NOT tagged and monitored by ThT fluorescence, and not a decrease in TMR fluorescence. Is this correct? " Abeta-42 is of Human origin based on the sequence given on aapptec web-site and APOE is of Human origin based on mutation mapping. Source of recombinant APOE traced to pmid:25207746 kanchan@tifrh.res.in Only protein-protein interactions imex curation in vitro In vitro fcs fluorescence correlation spectroscopy FCS FCS fcs predetermined predetermined participant predetermined Ghosh S., Sil TB., Dolai S., Garai K. Alzheimers - Interactions investigated in the context of Alzheimers disease email sent to authors 13/12/ regarding tags/method for fig 8A/B. ( no reply by 30/12/19 so sent for checking ) "Regarding Figure 8 A/B; Experiments are stated to be using TMR-Ab42 and monitored by ThT fluorescence, in the image text - under the image. However the images themselves do NOT mention TMR, and as TMR tagged Ab42 is stated in the Methods section to be monitored by a decrease in TMR fluorescence, it has been assumed that Ab42 is NOT tagged and monitored by ThT fluorescence, and not a decrease in TMR fluorescence. Is this correct? " Abeta-42 is of Human origin based on the sequence given on aapptec web-site and APOE is of Human origin based on mutation mapping. Source of recombinant APOE traced to pmid:25207746 kanchan@tifrh.res.in Accepted 2020-MAY-08 AT 05:26 BST AT 05:26 BST by KALPANAP The FEBS journal 2019 Only protein-protein interactions imex curation imex curation High-affinity multivalent interactions between apolipoprotein E and the oligomers of amyloid-β. High-affinity multivalent interactions between apolipoprotein E and the oligomers of amyloid-β. The FEBS journal 2019 imex curation Ghosh S., Sil TB., Dolai S., Garai K. Alzheimers - Interactions investigated in the context of Alzheimers disease email sent to authors 13/12/ regarding tags/method for fig 8A/B. ( no reply by 30/12/19 so sent for checking ) "Regarding Figure 8 A/B; Experiments are stated to be using TMR-Ab42 and monitored by ThT fluorescence, in the image text - under the image. However the images themselves do NOT mention TMR, and as TMR tagged Ab42 is stated in the Methods section to be monitored by a decrease in TMR fluorescence, it has been assumed that Ab42 is NOT tagged and monitored by ThT fluorescence, and not a decrease in TMR fluorescence. Is this correct? " Abeta-42 is of Human origin based on the sequence given on aapptec web-site and APOE is of Human origin based on mutation mapping. Source of recombinant APOE traced to pmid:25207746 kanchan@tifrh.res.in Only protein-protein interactions imex curation in vitro In vitro aggregation aggregation assay aggregation predetermined predetermined participant predetermined Ghosh S., Sil TB., Dolai S., Garai K. Alzheimers - Interactions investigated in the context of Alzheimers disease Figure 8; Experiments are stated to be using TMR-Ab42 in the image text, and monitored by ThT fluorescence. The image itself does not mention TMR and as TMR tagged Ab42 is monitored by a decrease in TMR fluorescence it has been assumed that Ab42 is NOT tagged. email sent to authors 13/12/ regarding tags/method for fig 8A/B. ( no reply by 30/12/19 so sent for checking ) "Regarding Figure 8 A/B; Experiments are stated to be using TMR-Ab42 and monitored by ThT fluorescence, in the image text - under the image. However the images themselves do NOT mention TMR, and as TMR tagged Ab42 is stated in the Methods section to be monitored by a decrease in TMR fluorescence, it has been assumed that Ab42 is NOT tagged and monitored by ThT fluorescence, and not a decrease in TMR fluorescence. Is this correct? " Amyloid formation was monitored by Thioflavin T (ThT) fluorescence which exhibits a fluorescence emission maximum at ~488nm exclusively when bound to amyloid. Abeta-42 is of Human origin based on the sequence given on aapptec web-site and APOE is of Human origin based on mutation mapping. Source of recombinant APOE traced to pmid:25207746 kanchan@tifrh.res.in Accepted 2020-MAY-08 AT 05:26 BST AT 05:26 BST by KALPANAP The FEBS journal 2019 Only protein-protein interactions imex curation imex curation High-affinity multivalent interactions between apolipoprotein E and the oligomers of amyloid-β. High-affinity multivalent interactions between apolipoprotein E and the oligomers of amyloid-β. The FEBS journal 2019 imex curation Ghosh S., Sil TB., Dolai S., Garai K. Alzheimers - Interactions investigated in the context of Alzheimers disease email sent to authors 13/12/ regarding tags/method for fig 8A/B. ( no reply by 30/12/19 so sent for checking ) "Regarding Figure 8 A/B; Experiments are stated to be using TMR-Ab42 and monitored by ThT fluorescence, in the image text - under the image. However the images themselves do NOT mention TMR, and as TMR tagged Ab42 is stated in the Methods section to be monitored by a decrease in TMR fluorescence, it has been assumed that Ab42 is NOT tagged and monitored by ThT fluorescence, and not a decrease in TMR fluorescence. Is this correct? " Abeta-42 is of Human origin based on the sequence given on aapptec web-site and APOE is of Human origin based on mutation mapping. Source of recombinant APOE traced to pmid:25207746 kanchan@tifrh.res.in Only protein-protein interactions imex curation in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Ghosh S., Sil TB., Dolai S., Garai K. Alzheimers - Interactions investigated in the context of Alzheimers disease email sent to authors 13/12/ regarding tags/method for fig 8A/B. ( no reply by 30/12/19 so sent for checking ) "Regarding Figure 8 A/B; Experiments are stated to be using TMR-Ab42 and monitored by ThT fluorescence, in the image text - under the image. However the images themselves do NOT mention TMR, and as TMR tagged Ab42 is stated in the Methods section to be monitored by a decrease in TMR fluorescence, it has been assumed that Ab42 is NOT tagged and monitored by ThT fluorescence, and not a decrease in TMR fluorescence. Is this correct? " Abeta-42 is of Human origin based on the sequence given on aapptec web-site and APOE is of Human origin based on mutation mapping. Source of recombinant APOE traced to pmid:25207746 kanchan@tifrh.res.in Accepted 2020-MAY-08 AT 05:26 BST AT 05:26 BST by KALPANAP The FEBS journal 2019 Only protein-protein interactions imex curation imex curation High-affinity multivalent interactions between apolipoprotein E and the oligomers of amyloid-β. High-affinity multivalent interactions between apolipoprotein E and the oligomers of amyloid-β. The FEBS journal 2019 imex curation Ghosh S., Sil TB., Dolai S., Garai K. Alzheimers - Interactions investigated in the context of Alzheimers disease email sent to authors 13/12/ regarding tags/method for fig 8A/B. ( no reply by 30/12/19 so sent for checking ) "Regarding Figure 8 A/B; Experiments are stated to be using TMR-Ab42 and monitored by ThT fluorescence, in the image text - under the image. However the images themselves do NOT mention TMR, and as TMR tagged Ab42 is stated in the Methods section to be monitored by a decrease in TMR fluorescence, it has been assumed that Ab42 is NOT tagged and monitored by ThT fluorescence, and not a decrease in TMR fluorescence. Is this correct? " Abeta-42 is of Human origin based on the sequence given on aapptec web-site and APOE is of Human origin based on mutation mapping. Source of recombinant APOE traced to pmid:25207746 kanchan@tifrh.res.in Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ghosh S., Sil TB., Dolai S., Garai K. Alzheimers - Interactions investigated in the context of Alzheimers disease email sent to authors 13/12/ regarding tags/method for fig 8A/B. ( no reply by 30/12/19 so sent for checking ) "Regarding Figure 8 A/B; Experiments are stated to be using TMR-Ab42 and monitored by ThT fluorescence, in the image text - under the image. However the images themselves do NOT mention TMR, and as TMR tagged Ab42 is stated in the Methods section to be monitored by a decrease in TMR fluorescence, it has been assumed that Ab42 is NOT tagged and monitored by ThT fluorescence, and not a decrease in TMR fluorescence. Is this correct? " Abeta-42 is of Human origin based on the sequence given on aapptec web-site and APOE is of Human origin based on mutation mapping. Source of recombinant APOE traced to pmid:25207746 kanchan@tifrh.res.in Accepted 2020-MAY-08 AT 05:26 BST AT 05:26 BST by KALPANAP The FEBS journal 2019 Only protein-protein interactions imex curation imex curation p02649-pro_0000001987 Apolipoprotein E APOE protein protein human Homo sapiens Human KVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH 19 317 E765BECDD0C05A55 p05067-pro_0000000092 Amyloid-beta protein 42 Alzheimer disease amyloid protein Cerebral vascular amyloid peptide APP A4 AD1 ABPP PreA4 Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 672 713 3AC85563D7858C37 app-app-125 4 8 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 1A-E, 8 A/B, Supp. fig. 2. app-app-121 4 8 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 7 inhibitor inhibitor unspecified role unspecified role purified purified NTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain CTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain HDF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P02649-PRO_0000001987:p.Cys112Arg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain C R Tue Jun 30 15:52:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys112Arg' ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 1A-E, 2, 8 A/B, supp. fig. 2 The inhibitory effect on aggregation of ab-42 is the highest with the full-length apoE4 followed by the NTF, CTF and HDF. Fragments of apoE (Apoe4) and full length Apoe3 and Apoe4, both in the lipid-free and in the lipidated forms, interact with Ab-42 to delay the kinetics of fibrillization. apoe-app-3 2 7 inhibitor inhibitor unspecified role unspecified role purified purified NTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain CTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain HDF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P02649-PRO_0000001987:p.Cys112Arg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain C R Tue Jun 30 17:50:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys112Arg' ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role neutral component neutral component n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 3, Supp. fig.3 The inhibitory effect on aggregation of ab-42 is the highest with the full-length apoE4 followed by the NTF, CTF and HDF. Both apoE3 and apoE4 delay the kinetics of aggregation of Ab42 both in lipid-free as well as in lipidated forms. app-app-126 2 8 unspecified role unspecified role neutral component neutral component n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role neutral component neutral component n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 3, Supp. fig. 3 apoe-app-4 5 7 unspecified role unspecified role fluorescence donor fluorescence donor purified purified NTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain CTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain HDF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P02649-PRO_0000001987:p.Cys112Arg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain C R Tue Jun 30 16:19:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys112Arg' ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept edans_label fluorophore fluorophore n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept edans_label fluorophore fluorophore n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 5A, 8C, Supp. fig 10. The APOE*4 variant :P02649 VAR_000652. Native tryptophan residues in apoE are the FRET donor and EDANS tagged to ab-42 is the acceptor. The slow time-dependent increase of FRET is consistent with interactions of apoE with species of Ab42 such as the ‘oligomers’ that form in a time-dependent manner. apoe-app-5 5 7 unspecified role unspecified role fluorescence donor fluorescence donor purified purified edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain P02649-PRO_0000001987:p.Ala62Cys mutation mutation certain certain sequence position certain certain certain sequence position certain A C Tue Jun 30 18:21:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala62Cys' edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain P02649-PRO_0000001987:p.Cys112Arg mutation mutation certain certain sequence position certain certain certain sequence position certain C R Tue Jun 30 17:13:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys112Arg' P02649-PRO_0000001987:p.Ser139Cys mutation mutation certain certain sequence position certain certain certain sequence position certain S C Tue Jun 30 18:43:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser139Cys' edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain P02649-PRO_0000001987:p.Trp210Cys mutation mutation certain certain sequence position certain certain certain sequence position certain W C Tue Jun 30 18:45:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp210Cys' edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain P02649-PRO_0000001987:p.Ala247Cys mutation mutation certain certain sequence position certain certain certain sequence position certain A C Tue Jun 30 16:06:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala247Cys' edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain P02649-PRO_0000001987:p.Trp276Cys mutation mutation certain certain sequence position certain certain certain sequence position certain W C Tue Jun 30 19:20:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp276Cys' ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 48 47 49 51 53 52 55 54 56 57 direct interaction direct interaction 5B, Supp. fig 6 The APOE*4 variant :P02649 VAR_000652. Figure 5B shows that the kinetics of FRET efficiency between apoE and Ab are similar for all the five cysteine mutants. Similar results are obtained with EDANS-labelled lipidated apoE (Suppl Fig. 6) apoe-app-6 5 7 unspecified role unspecified role fluorescence donor fluorescence donor purified purified P02649-PRO_0000001987:p.Ala247Cys mutation mutation certain certain sequence position certain certain certain sequence position certain A C Tue Jun 30 17:02:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala247Cys' edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain P02649-PRO_0000001987:p.Cys112Arg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain C R Tue Jun 30 19:43:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys112Arg' ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 64 65 direct interaction direct interaction 6A,7A, 8D, Supp. fig 7,8. The APOE*4 variant :P02649 VAR_000652. It has been assumed the APOE mutation "W247C" is a typo and was meant to be A247C. Figure 7:FRET is measured as a decrease in donor fluorescence at t=14hrs, rather than FRET efficiency. apoe-app-7 5 7 competitor competitor competitor unspecified role unspecified role purified purified P02649-PRO_0000001987:p.Cys112Arg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain C R Tue Jun 30 16:06:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys112Arg' ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role fluorescence donor fluorescence donor purified purified edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain P02649-PRO_0000001987:p.Ala247Cys mutation mutation certain certain sequence position certain certain certain sequence position certain A C Tue Jun 30 20:10:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala247Cys' P02649-PRO_0000001987:p.Cys112Arg mutation mutation certain certain sequence position certain certain certain sequence position certain C R Tue Jun 30 16:06:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys112Arg' ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 76 75 direct interaction direct interaction 6A-2, 7A-2, 8D, Supp. Fig. 7 The APOE*4 variant :P02649 VAR_000652. It has been assumed the APOE mutation "W247C" is a typo and was meant to be A247C. The reduction of the FRET signal is very small in the presence of the (unlabelled) APOE4 fragments but high in the presence of the full-length apoE4. Figure 7: FRET is measured as a decrease in donor fluorescence at t=14hrs, rather than FRET efficiency. This effect is reversed in the presence of a competitor, full length APOE, but less so in the presence of NTF and CTF mixed together. apoe-app-9 5 7 unspecified role unspecified role fluorescence donor fluorescence donor purified purified P02649-PRO_0000001987:p.Ala247Cys mutation mutation certain certain sequence position certain certain certain sequence position certain A C Tue Jun 30 19:03:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala247Cys' edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain CTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role fluorescence donor fluorescence donor purified purified P02649-PRO_0000001987:p.Cys112Arg mutation mutation certain certain sequence position certain certain certain sequence position certain C R Tue Jun 30 20:22:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys112Arg' P02649-PRO_0000001987:p.Ala102Cys mutation mutation certain certain sequence position certain certain certain sequence position certain A C Tue Jun 30 16:06:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala102Cys' edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain NTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 85 84 94 93 direct interaction direct interaction 6B,7B The APOE*4 variant :P02649 VAR_000652. It has been assumed the APOE mutation "W247C" is a typo and was meant to be A247C. there may be a better way to show fig6B without competitor? Figure 7:FRET is measured as a decrease in donor fluorescence rather than FRET efficiency. apoe-app-10 5 7 competitor competitor competitor unspecified role unspecified role purified purified ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role fluorescence donor fluorescence donor purified purified P02649-PRO_0000001987:p.Ala247Cys mutation mutation certain certain sequence position certain certain certain sequence position certain A C Tue Jun 30 19:57:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala247Cys' edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain CTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept purified purified n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept purified purified n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role fluorescence donor fluorescence donor purified purified P02649-PRO_0000001987:p.Cys112Arg mutation mutation certain certain sequence position certain certain certain sequence position certain C R Tue Jun 30 16:59:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys112Arg' P02649-PRO_0000001987:p.Ala102Cys mutation mutation certain certain sequence position certain certain certain sequence position certain A C Tue Jun 30 16:41:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala102Cys' edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain NTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 99 100 108 109 direct interaction direct interaction 6B-2,7B-2 The APOE*4 variant :P02649 VAR_000652. It has been assumed the APOE mutation "W247C" is a typo and was meant to be A247C. FRET is reduced strongly in the presence of unlabelled full-length apoE4 but affected modestly in the presence of unlabelled NTF or CTF or NTF + CTF. 6B:I have only entered fragment competitors as a comment, as it is unclear what the green triangles are: image says NTF +CTF, image text says NTF alone. Also any effect is small. Figure 7:FRET is measured as a decrease in donor fluorescence rather than FRET efficiency. apoe-app-11 5 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role fluorescence donor fluorescence donor purified purified P02649-PRO_0000001987:p.Cys112Arg mutation mutation certain certain sequence position certain certain certain sequence position certain C R Tue Jun 30 20:16:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys112Arg' P02649-PRO_0000001987:p.Ala102Cys mutation mutation certain certain sequence position certain certain certain sequence position certain A C Tue Jun 30 20:14:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala102Cys' edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain NTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 119 118 direct interaction direct interaction 6C The APOE*4 variant :P02649 VAR_000652. apoe-app-12 5 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role fluorescence donor fluorescence donor purified purified P02649-PRO_0000001987:p.Ala102Cys mutation mutation certain certain sequence position certain certain certain sequence position certain A C Tue Jun 30 16:57:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala102Cys' edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain NTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P02649-PRO_0000001987:p.Cys112Arg mutation mutation certain certain sequence position certain certain certain sequence position certain C R Tue Jun 30 15:51:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys112Arg' ecoli Escherichia coli (strain K12) 7 competitor competitor competitor unspecified role unspecified role purified purified P02649-PRO_0000001987:p.Cys112Arg mutation mutation certain certain sequence position certain certain certain sequence position certain C R Tue Jun 30 19:14:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys112Arg' ecoli Escherichia coli (strain K12) 127 128 direct interaction direct interaction 6C-2 The APOE*4 variant :P02649 VAR_000652. apoe-app-13 5 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role fluorescence donor fluorescence donor purified purified P02649-PRO_0000001987:p.Ala102Cys mutation mutation certain certain sequence position certain certain certain sequence position certain A C Tue Jun 30 20:05:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala102Cys' edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain NTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P02649-PRO_0000001987:p.Cys112Arg mutation mutation certain certain sequence position certain certain certain sequence position certain C R Tue Jun 30 16:45:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys112Arg' ecoli Escherichia coli (strain K12) 7 stimulator stimulator unspecified role unspecified role purified purified CTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 139 140 direct interaction direct interaction 6C-3 The APOE*4 variant :P02649 VAR_000652. The presence of an excess amount of CTF appears to enhance the FRET between the EDANS-NTF and TMR-Ab42. apoe-app-14 5 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role fluorescence donor fluorescence donor purified purified P02649-PRO_0000001987:p.Cys112Arg mutation mutation certain certain sequence position certain certain certain sequence position certain C R Tue Jun 30 20:04:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys112Arg' P02649-PRO_0000001987:p.Ala102Cys mutation mutation certain certain sequence position certain certain certain sequence position certain A C Tue Jun 30 20:09:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala102Cys' edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain NTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 competitor competitor competitor unspecified role unspecified role purified purified P02649-PRO_0000001987:p.Cys112Arg mutation mutation certain certain sequence position certain certain certain sequence position certain C R Tue Jun 30 19:03:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys112Arg' NTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 152 153 direct interaction direct interaction 6C-4 The APOE*4 variant :P02649 VAR_000652. The NTF fragment is a less effective competitor than the full length protein. apoe-app-15 5 7 unspecified role unspecified role fluorescence donor fluorescence donor purified purified P02649-PRO_0000001987:p.Ala247Cys mutation mutation certain certain sequence position certain certain certain sequence position certain A C Tue Jun 30 15:45:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala247Cys' edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain CTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 160 161 direct interaction direct interaction 6D The APOE*4 variant :P02649 VAR_000652. apoe-app-16 5 7 competitor competitor competitor unspecified role unspecified role purified purified ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role fluorescence donor fluorescence donor purified purified P02649-PRO_0000001987:p.Ala247Cys mutation mutation certain certain sequence position certain certain certain sequence position certain A C Tue Jun 30 17:44:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala247Cys' edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain CTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 171 170 direct interaction direct interaction 6D-2 The APOE*4 variant :P02649 VAR_000652. apoe-app-17 5 7 competitor competitor competitor unspecified role unspecified role purified purified P02649-PRO_0000001987:p.Cys112Arg mutation mutation certain certain sequence position certain certain certain sequence position certain C R Tue Jun 30 19:43:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys112Arg' NTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role fluorescence donor fluorescence donor purified purified P02649-PRO_0000001987:p.Ala247Cys mutation mutation certain certain sequence position certain certain certain sequence position certain A C Tue Jun 30 19:52:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala247Cys' edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain CTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 183 182 direct interaction direct interaction 6D-3 The APOE*4 variant :P02649 VAR_000652. The CTF and NTF fragments are less effective competitors than full length APOE. apoe-app-18 5 7 competitor competitor competitor unspecified role unspecified role purified purified CTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role fluorescence donor fluorescence donor purified purified P02649-PRO_0000001987:p.Ala247Cys mutation mutation certain certain sequence position certain certain certain sequence position certain A C Tue Jun 30 20:21:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala247Cys' edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain CTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 193 194 direct interaction direct interaction 6D-4 The APOE*4 variant :P02649 VAR_000652. The CTF and NTF fragments are less effective competitors than full length APOE. apoe-app-8 1 7 inhibitor inhibitor unspecified role unspecified role purified purified NTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain CTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain HDF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P02649-PRO_0000001987:p.Cys112Arg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain C R Tue Jun 30 15:43:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys112Arg' ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Supp. fig. 1 The inhibitory effect on aggregation of ab-42 is the highest with the full-length apoE4 followed by the NTF, CTF and HDF. Fragments of apoE (Apoe4) and full length Apoe3 and Apoe4, both in the lipid-free and in the lipidated forms, interact with Ab-42 to delay the kinetics of fibrillization. app-app-127 1 8 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Supp. fig. 1. app-app-128 1 8 unspecified role unspecified role neutral component neutral component edans_label fluorophore fluorophore n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role neutral component neutral component edans_label fluorophore fluorophore n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Supp. fig. 5. apoe-app-19 6 7 unspecified role unspecified role neutral component neutral component purified purified P02649-PRO_0000001987:p.Cys112Arg mutation mutation certain certain sequence position certain certain certain sequence position certain C R Tue Jun 30 15:51:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys112Arg' ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role neutral component neutral component n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Supp. fig.9D apoe-app-20 3 7 unspecified role unspecified role neutral component neutral component purified purified P02649-PRO_0000001987:p.Cys112Arg mutation mutation certain certain sequence position certain certain certain sequence position certain C R Tue Jun 30 17:24:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys112Arg' ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role neutral component neutral component n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Supp. fig.9E-I apoE-Ab form complexes that are highly heterogeneous and oligomeric in nature.