UniProt UniProt http://www.uniprot.org Physical and Functional Interaction between 5-HT6 Receptor and Nova-1. Physical and Functional Interaction between 5-HT6 Receptor and Nova-1. Experimental Neurobiology 2019 imex curation Kim SH., Seo M., Hwang H., Moon DM., Son GH., Kim K., Rhim H. hrhim@kist.re.kr 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation rat-brain Rattus norvegicus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kim SH., Seo M., Hwang H., Moon DM., Son GH., Kim K., Rhim H. hrhim@kist.re.kr Accepted 2019-DEC-02 AT 09:12 GMT AT 09:12 GMT by MGT Experimental Neurobiology 2019 Only protein-protein interactions imex curation imex curation Physical and Functional Interaction between 5-HT6 Receptor and Nova-1. Physical and Functional Interaction between 5-HT6 Receptor and Nova-1. Experimental Neurobiology 2019 imex curation Kim SH., Seo M., Hwang H., Moon DM., Son GH., Kim K., Rhim H. hrhim@kist.re.kr 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Kim SH., Seo M., Hwang H., Moon DM., Son GH., Kim K., Rhim H. hrhim@kist.re.kr Accepted 2019-DEC-02 AT 09:12 GMT AT 09:12 GMT by MGT Experimental Neurobiology 2019 Only protein-protein interactions imex curation imex curation Physical and Functional Interaction between 5-HT6 Receptor and Nova-1. Physical and Functional Interaction between 5-HT6 Receptor and Nova-1. Experimental Neurobiology 2019 imex curation Kim SH., Seo M., Hwang H., Moon DM., Son GH., Kim K., Rhim H. hrhim@kist.re.kr 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kim SH., Seo M., Hwang H., Moon DM., Son GH., Kim K., Rhim H. hrhim@kist.re.kr Accepted 2019-DEC-02 AT 09:12 GMT AT 09:12 GMT by MGT Experimental Neurobiology 2019 Only protein-protein interactions imex curation imex curation Physical and Functional Interaction between 5-HT6 Receptor and Nova-1. Physical and Functional Interaction between 5-HT6 Receptor and Nova-1. Experimental Neurobiology 2019 imex curation Kim SH., Seo M., Hwang H., Moon DM., Son GH., Kim K., Rhim H. hrhim@kist.re.kr 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Kim SH., Seo M., Hwang H., Moon DM., Son GH., Kim K., Rhim H. hrhim@kist.re.kr Accepted 2019-DEC-02 AT 09:12 GMT AT 09:12 GMT by MGT Experimental Neurobiology 2019 Only protein-protein interactions imex curation imex curation Physical and Functional Interaction between 5-HT6 Receptor and Nova-1. Physical and Functional Interaction between 5-HT6 Receptor and Nova-1. Experimental Neurobiology 2019 imex curation Kim SH., Seo M., Hwang H., Moon DM., Son GH., Kim K., Rhim H. hrhim@kist.re.kr 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kim SH., Seo M., Hwang H., Moon DM., Son GH., Kim K., Rhim H. hrhim@kist.re.kr Accepted 2019-DEC-02 AT 09:12 GMT AT 09:12 GMT by MGT Experimental Neurobiology 2019 Only protein-protein interactions imex curation imex curation Physical and Functional Interaction between 5-HT6 Receptor and Nova-1. Physical and Functional Interaction between 5-HT6 Receptor and Nova-1. Experimental Neurobiology 2019 imex curation Kim SH., Seo M., Hwang H., Moon DM., Son GH., Kim K., Rhim H. hrhim@kist.re.kr 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-gt1_7 Mus musculus hypothalamic cell line, GT1-7 gt1_7 mouse hypothalamic cell line confocal microscopy confocal microscopy anti-tag immunocytochemistry anti-tag immunocytochemistry Kim SH., Seo M., Hwang H., Moon DM., Son GH., Kim K., Rhim H. hrhim@kist.re.kr Accepted 2019-DEC-02 AT 09:12 GMT AT 09:12 GMT by MGT Experimental Neurobiology 2019 Only protein-protein interactions imex curation imex curation Physical and Functional Interaction between 5-HT6 Receptor and Nova-1. Physical and Functional Interaction between 5-HT6 Receptor and Nova-1. Experimental Neurobiology 2019 imex curation Kim SH., Seo M., Hwang H., Moon DM., Son GH., Kim K., Rhim H. hrhim@kist.re.kr 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Kim SH., Seo M., Hwang H., Moon DM., Son GH., Kim K., Rhim H. hrhim@kist.re.kr Accepted 2019-DEC-02 AT 09:12 GMT AT 09:12 GMT by MGT Experimental Neurobiology 2019 Only protein-protein interactions imex curation imex curation Physical and Functional Interaction between 5-HT6 Receptor and Nova-1. Physical and Functional Interaction between 5-HT6 Receptor and Nova-1. Experimental Neurobiology 2019 imex curation Kim SH., Seo M., Hwang H., Moon DM., Son GH., Kim K., Rhim H. hrhim@kist.re.kr 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. confocal microscopy confocal microscopy anti-tag immunocytochemistry anti-tag immunocytochemistry Kim SH., Seo M., Hwang H., Moon DM., Son GH., Kim K., Rhim H. hrhim@kist.re.kr Accepted 2019-DEC-02 AT 09:12 GMT AT 09:12 GMT by MGT Experimental Neurobiology 2019 Only protein-protein interactions imex curation imex curation Physical and Functional Interaction between 5-HT6 Receptor and Nova-1. Physical and Functional Interaction between 5-HT6 Receptor and Nova-1. Experimental Neurobiology 2019 imex curation Kim SH., Seo M., Hwang H., Moon DM., Son GH., Kim K., Rhim H. hrhim@kist.re.kr 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kim SH., Seo M., Hwang H., Moon DM., Son GH., Kim K., Rhim H. hrhim@kist.re.kr Accepted 2019-DEC-02 AT 09:12 GMT AT 09:12 GMT by MGT Experimental Neurobiology 2019 Only protein-protein interactions imex curation imex curation Physical and Functional Interaction between 5-HT6 Receptor and Nova-1. Physical and Functional Interaction between 5-HT6 Receptor and Nova-1. Experimental Neurobiology 2019 imex curation Kim SH., Seo M., Hwang H., Moon DM., Son GH., Kim K., Rhim H. hrhim@kist.re.kr 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Kim SH., Seo M., Hwang H., Moon DM., Son GH., Kim K., Rhim H. hrhim@kist.re.kr Accepted 2019-DEC-02 AT 09:12 GMT AT 09:12 GMT by MGT Human brain cDNA Experimental Neurobiology 2019 Only protein-protein interactions imex curation imex curation 5ht6r_human 5-hydroxytryptamine receptor 6 Serotonin receptor 6 HTR6 protein protein human Homo sapiens Human MVPEPGPTANSTPAWGAGPPSAPGGSGWVAAALCVVIALTAAANSLLIALICTQPALRNTSNFFLVSLFTSDLMVGLVVMPPAMLNALYGRWVLARGLCLLWTAFDVMCCSASILNLCLISLDRYLLILSPLRYKLRMTPLRALALVLGAWSLAALASFLPLLLGWHELGHARPPVPGQCRLLASLPFVLVASGLTFFLPSGAICFTYCRILLAARKQAVQVASLTTGMASQASETLQVPRTPRPGVESADSRRLATKHSRKALKASLTLGILLGMFFVTWLPFFVANIVQAVCDCISPGLFDVLTWLGYCNSTMNPIIYPLFMRDFKRALGRFLPCPRCPRERQASLASPSLRTSHSGPRPGLSLQQVLPLPLPPDSDSDSDAGSGGSSGLRLTAQLLLPGEATQDPPLPTRAAAAVNFFNIDPAEPELRPHPLGIPTN C888F47650C1D2EF 5ht6r_rat 5-hydroxytryptamine receptor 6 ST-B17 Htr6 Serotonin receptor 6 protein protein rat Rattus norvegicus (Rat) Rat MVPEPGPVNSSTPAWGPGPPPAPGGSGWVAAALCVVIVLTAAANSLLIVLICTQPALRNTSNFFLVSLFTSDLMVGLVVMPPAMLNALYGRWVLARGLCLLWTAFDVMCCSASILNLCLISLDRYLLILSPLRYKLRMTAPRALALILGAWSLAALASFLPLLLGWHELGKARTPAPGQCRLLASLPFVLVASGVTFFLPSGAICFTYCRILLAARKQAVQVASLTTGTAGQALETLQVPRTPRPGMESADSRRLATKHSRKALKASLTLGILLGMFFVTWLPFFVANIAQAVCDCISPGLFDVLTWLGYCNSTMNPIIYPLFMRDFKRALGRFLPCVHCPPEHRPALPPPPCGPLTAVPDQASACSRCCLCLCRQTQIQTPLQGAPRACSSQPSFCCLERPPGTPRHPPGPPLWSTSLSQTLWSLRYGRIHSVPP 6BC00F6A3CBA5FB4 nova1_human RNA-binding protein Nova-1 Neuro-oncological ventral antigen 1 Onconeural ventral antigen 1 Paraneoplastic Ri antigen Ventral neuron-specific protein 1 NOVA1 protein protein human Homo sapiens Human MMAAAPIQQNGTHTGVPIDLDPPDSRKRPLEAPPEAGSTKRTNTGEDGQYFLKVLIPSYAAGSIIGKGGQTIVQLQKETGATIKLSKSKDFYPGTTERVCLIQGTVEALNAVHGFIAEKIREMPQNVAKTEPVSILQPQTTVNPDRIKQTLPSSPTTTKSSPSDPMTTSRANQVKIIVPNSTAGLIIGKGGATVKAVMEQSGAWVQLSQKPDGINLQERVVTVSGEPEQNRKAVELIIQKIQEDPQSGSCLNISYANVTGPVANSNPTGSPYANTAEVLPTAAAAAGLLGHANLAGVAAFPAVLSGFTGNDLVAITSALNTLASYGYNLNTLGLGLSQAAATGALAAAAASANPAAAAANLLATYASEASASGSTAGGTAGTFALGSLAAATAATNGYFGAASPLAASAILGTEKSTDGSKDVVEIAVPENLVGAILGKGGKTLVEYQELTGARIQISKKGEFVPGTRNRKVTITGTPAATQAAQYLITQRITYEQGVRAANPQKVG DBF455477988CDCA nova1_rat RNA-binding protein Nova-1 Nova1 Neuro-oncological ventral antigen 1 protein protein rat Rattus norvegicus (Rat) Rat MAAAPIQQNGTHTGVPIDLDPPDSRKRPLEAPPEAGSTKRTNTGEDGQYFLKVLIPSYAAGSIIGKGGQTIVQLQKETGATIKLSKSKDFYPGTTERVCLIQGTIEALNAVHGFIAEKIREMPQNVAKTEPVSILQPQTTVNPDRIKQVKIIVPNSTAGLIIGKGGATVKAIMEQSGAWVQLSQKPDGINLQERVVTVSGEPEQNRKAVELIIQKIQEDPQSGSCLNISYANVTGPVANSNPTGFPYANTAEVLPTAAAAAGLLGHANLAGVAAFPAVLSGFTGNDLVAITSALNTLASYGYNLNTLGLGLSQAAATGALAAAAASANPAAAAANLLATYASEASASGSTAGGTAGTFALGSLAAATAATNGYFGAASPLAASAILGTEKSTDGSKDVVEIAVPENLVGAILGKGGKTLVEYQELTGARIQISKKGEFVPGTRNRKVTITGTPAATQAAQYLITQRITYEQGVR 70D0926B5BECD131 nova1_mouse RNA-binding protein Nova-1 Nova1 Neuro-oncological ventral antigen 1 Ventral neuron-specific protein 1 protein protein mouse Mus musculus Mouse MMAAAPIQQNGTHTGVPIDLDPPDSRKRPLEAPPEAGSTKRTNTGEDGQYFLKVLIPSYAAGSIIGKGGQTIVQLQKETGATIKLSKSKDFYPGTTERVCLIQGTIEALNAVHGFIAEKIREMPQNVAKTEPVSILQPQTTVNPDRIKQTLPSSPTTTKSSPSDPMTTSRANQVKIIVPNSTAGLIIGKGGATVKAIMEQSGAWVQLSQKPDGINLQERVVTVSGEPEQNRKAVELIIQKIQEDPQSGSCLNISYANVTGPVANSNPTGSPYANTAEVLPTAAAAAGLLGHANLAGVAAFPAVLSGFTGNDLVAITSALNTLASYGYNLNTLGLGLSQAAATGALAAAAASANPAAAAANLLATYASEASASGSTAGGTAGTFALGSLAAATAATNGYFGAASPLAASAILGTEKSTDGSKDVVEIAVPENLVGAILGKGGKTLVEYQELTGARIQISKKGEFVPGTRNRKVTITGTPAATQAAQYLITQRITYEQGVRAANPQKVG mint Functions to regulate alternative splicing in neurons by binding pre-mRNA in a sequence-specific manner to activate exon inclusion. It binds specifically to the sequence UCAUY. Most likely acts to activate the inclusion of exon E3A in the glycine receptor alpha-2 chain and of exon E9 in gamma-aminobutyric-acid receptor gamma-2 subunit via a distal downstream UCAU-rich intronic splicing enhancer Defects in Nova1 leads to neuronal death in spinal and brainstem neurons EB68F2D777BF8135 htr6-nova1-1 10 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c-termial region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 13 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association htr6-nova1-2 9 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 13 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association 1B htr6-nova1-3 2 11 unspecified role unspecified role bait bait purified purified c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association Fig. 1C, 1I nova1-htr6-1 3 11 unspecified role unspecified role prey prey purified purified c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 13 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 1D htr6-nova1-8 3 11 unspecified role unspecified role bait bait purified purified c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 13 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 1E htr6-nova1-4 7 11 unspecified role unspecified role bait bait purified purified c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level rat-brain Rattus norvegicus brain brain Brain [cerebrum] physical association physical association Fig. 1G htr6-nova1-5 1 12 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 14 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Fig. 1H htr6-nova1-6 2 11 unspecified role unspecified role bait bait purified purified c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal venus-c venus c-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association Fig. 2C nova1-htr6-2 5 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal venus-c venus c-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association Fig. 2D htr6-nova1-7 4 12 unspecified role unspecified role bait bait cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level rat-brain Rattus norvegicus brain brain Brain [cerebrum] 13 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal venus-c venus c-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association Fig. 2E nova1-htr6-3 2 15 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association Fig. 2F htr6-nova1-9 6 11 unspecified role unspecified role neutral component neutral component purified purified n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 13 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization Fig. 4B htr6-nova1-10 8 11 unspecified role unspecified role neutral component neutral component purified purified n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 13 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization Fig. 4C