DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural insights into the activation of metabotropic glutamate receptors. Structural insights into the activation of metabotropic glutamate receptors. Nature 2019 imex curation Koehl A., Hu H., Feng D., Sun B., Zhang Y., Robertson MJ., Chu M., Kobilka TS., Laermans T., Steyaert J., Tarrasch J., Dutta S., Fonseca R., Weis WI., Mathiesen JM., Skiniotis G., Kobilka BK. jmm@sund.ku.dk 30-Aug-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction experimental particp experimental participant identification experimental particp Koehl A., Hu H., Feng D., Sun B., Zhang Y., Robertson MJ., Chu M., Kobilka TS., Laermans T., Steyaert J., Tarrasch J., Dutta S., Fonseca R., Weis WI., Mathiesen JM., Skiniotis G., Kobilka BK. jmm@sund.ku.dk Accepted 2019-AUG-01 AT 11:40 BST AT 11:40 BST by ORCHARD Nature 2019 imex curation Structural insights into the activation of metabotropic glutamate receptors. Structural insights into the activation of metabotropic glutamate receptors. Nature 2019 imex curation Koehl A., Hu H., Feng D., Sun B., Zhang Y., Robertson MJ., Chu M., Kobilka TS., Laermans T., Steyaert J., Tarrasch J., Dutta S., Fonseca R., Weis WI., Mathiesen JM., Skiniotis G., Kobilka BK. jmm@sund.ku.dk 30-Aug-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction experimental particp experimental participant identification experimental particp Koehl A., Hu H., Feng D., Sun B., Zhang Y., Robertson MJ., Chu M., Kobilka TS., Laermans T., Steyaert J., Tarrasch J., Dutta S., Fonseca R., Weis WI., Mathiesen JM., Skiniotis G., Kobilka BK. jmm@sund.ku.dk Accepted 2019-AUG-01 AT 11:40 BST AT 11:40 BST by ORCHARD Nature 2019 imex curation Structural insights into the activation of metabotropic glutamate receptors. Structural insights into the activation of metabotropic glutamate receptors. Nature 2019 imex curation Koehl A., Hu H., Feng D., Sun B., Zhang Y., Robertson MJ., Chu M., Kobilka TS., Laermans T., Steyaert J., Tarrasch J., Dutta S., Fonseca R., Weis WI., Mathiesen JM., Skiniotis G., Kobilka BK. jmm@sund.ku.dk 30-Aug-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 3d-em 3D electron microscopy 3D-EM electron tomog experimental particp experimental participant identification experimental particp Koehl A., Hu H., Feng D., Sun B., Zhang Y., Robertson MJ., Chu M., Kobilka TS., Laermans T., Steyaert J., Tarrasch J., Dutta S., Fonseca R., Weis WI., Mathiesen JM., Skiniotis G., Kobilka BK. jmm@sund.ku.dk Accepted 2019-AUG-01 AT 11:40 BST AT 11:40 BST by ORCHARD Nature 2019 imex curation grm5_human Metabotropic glutamate receptor 5 GRM5 GPRC1E MGLUR5 protein protein human Homo sapiens Human MVLLLILSVLLLKEDVRGSAQSSERRVVAHMPGDIIIGALFSVHHQPTVDKVHERKCGAVREQYGIQRVEAMLHTLERINSDPTLLPNITLGCEIRDSCWHSAVALEQSIEFIRDSLISSEEEEGLVRCVDGSSSSFRSKKPIVGVIGPGSSSVAIQVQNLLQLFNIPQIAYSATSMDLSDKTLFKYFMRVVPSDAQQARAMVDIVKRYNWTYVSAVHTEGNYGESGMEAFKDMSAKEGICIAHSYKIYSNAGEQSFDKLLKKLTSHLPKARVVACFCEGMTVRGLLMAMRRLGLAGEFLLLGSDGWADRYDVTDGYQREAVGGITIKLQSPDVKWFDDYYLKLRPETNHRNPWFQEFWQHRFQCRLEGFPQENSKYNKTCNSSLTLKTHHVQDSKMGFVINAIYSMAYGLHNMQMSLCPGYAGLCDAMKPIDGRKLLESLMKTNFTGVSGDTILFDENGDSPGRYEIMNFKEMGKDYFDYINVGSWDNGELKMDDDEVWSKKSNIIRSVCSEPCEKGQIKVIRKGEVSCCWTCTPCKENEYVFDEYTCKACQLGSWPTDDLTGCDLIPVQYLRWGDPEPIAAVVFACLGLLATLFVTVVFIIYRDTPVVKSSSRELCYIILAGICLGYLCTFCLIAKPKQIYCYLQRIGIGLSPAMSYSALVTKTNRIARILAGSKKKICTKKPRFMSACAQLVIAFILICIQLGIIVALFIMEPPDIMHDYPSIREVYLICNTTNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITMCFSVSLSATVALGCMFVPKVYIILAKPERNVRSAFTTSTVVRMHVGDGKSSSAASRSSSLVNLWKRRGSSGETLRYKDRRLAQHKSEIECFTPKGSMGNGGRATMSSSNGKSVTWAQNEKSSRGQHLWQRLSIHINKKENPNQTAVIKPFPKSTESRGLGAGAGAGGSAGGVGATGGAGCAGAGPGGPESPDAGPKALYDVAEAEEHFPAPARPRSPSPISTLSHRAGSASRTDDDVPSLHSEPVARSSSSQGSLMEQISSVVTRFTANISELNSMMLSTAAPSPGVGAPLCSSYLIPKEIQLPTTMTTFAEIQPLPAIEVTGGAQPAAGAQAAGDAARESPAAGPEAAAAKPDLEELVALTPPSPFRDSVDSGSTTPNSPVSESALCIPSSPKYDTLIIRDYTQSSSSL A3C73606681C6A25 cryo em-4 3 4 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified HA tag HA tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Flag tag Flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range Binding region Binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction Fig 3 grm5-1 1 4 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified Binding region Binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig 2. Ex Data Tab 1 grm5-17 2 4 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified HA tag HA tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Flag tag Flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range Binding region Binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction Fig 2. Ex Data Tab 1