DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu GABAA receptor signalling mechanisms revealed by structural pharmacology. GABAA receptor signalling mechanisms revealed by structural pharmacology. Nature 2019 imex curation Masiulis S., Desai R., Uchański T., Serna Martin I., Laverty D., Karia D., Malinauskas T., Zivanov J., Pardon E., Kotecha A., Steyaert J., Miller KW., Aricescu AR. radu@mrc-lmb.cam.ac.uk 30-Aug-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293s Human 293S cell line Human 293s 293S cell line 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Masiulis S., Desai R., Uchański T., Serna Martin I., Laverty D., Karia D., Malinauskas T., Zivanov J., Pardon E., Kotecha A., Steyaert J., Miller KW., Aricescu AR. radu@mrc-lmb.cam.ac.uk Accepted 2019-JUL-29 AT 13:45 BST AT 13:45 BST by ORCHARD Nature 2019 imex curation p18507-2 Gamma-aminobutyric acid receptor subunit gamma-2 GABRG2 GABA(A) receptor subunit gamma-2 protein protein human Homo sapiens Human MSSPNIWSTGSSVYSTPVFSQKMTVWILLLLSLYPGFTSQKSDDDYEDYASNKTWVLTPKVPEGDVTVILNNLLEGYDNKLRPDIGVKPTLIHTDMYVNSIGPVNAINMEYTIDIFFAQTWYDRRLKFNSTIKVLRLNSNMVGKIWIPDTFFRNSKKADAHWITTPNRMLRIWNDGRVLYTLRLTIDAECQLQLHNFPMDEHSCPLEFSSYGYPREEIVYQWKRSSVEVGDTRSWRLYQFSFVGLRNTTEVVKTTSGDYVVMSVYFDLSRRMGYFTIQTYIPCTLIVVLSWVSFWINKDAVPARTSLGITTVLTMTTLSTIARKSLPKVSYVTAMDLFVSVCFIFVFSALVEYGTLHYFVSNRKPSKDKDKKKKNPLLRMFSFKAPTIDIRPRSATIQMNNATHLQERDEEYGYECLDGKDCASFFCCFEDCRTGAWRHGRIHIRIAKMDSYARIFFPTAFCLFNLVYWVSYLYL DE142F62846576DF p28472-2 Gamma-aminobutyric acid receptor subunit beta-3 GABA(A) receptor subunit beta-3 GABRB3 protein protein human Homo sapiens Human MCSGLLELLLPIWLSWTLGTRGSEPRSVNDPGNMSFVKETVDKLLKGYDIRLRPDFGGPPVCVGMNIDIASIDMVSEVNMDYTLTMYFQQYWRDKRLAYSGIPLNLTLDNRVADQLWVPDTYFLNDKKSFVHGVTVKNRMIRLHPDGTVLYGLRITTTAACMMDLRRYPLDEQNCTLEIESYGYTTDDIEFYWRGGDKAVTGVERIELPQFSIVEHRLVSRNVVFATGAYPRLSLSFRLKRNIGYFILQTYMPSILITILSWVSFWINYDASAARVALGITTVLTMTTINTHLRETLPKIPYVKAIDMYLMGCFVFVFLALLEYAFVNYIFFGRGPQRQKKLAEKTAKAKNDRSKSESNRVDAHGNILLTSLEVHNEMNEVSGGIGDTRNSAISFDNSGIQYRKQSMPREGHGRFLGDRSLPHKKTHLRRRSSQLKIKIPDLTDVNAIDRWSRIVFPFTFSLFNLVYWLYYVN Variant in position: 11:P->S (in ECA5, the mutant protein is hyperglycosylated and has reduced mean current densities compared to wild-type, dbSNP:rs25409). Variant in position: 15:S->F (in ECA5, the mutant protein is hyperglycosylated and has reduced mean current densities compared to wild-type). 3A7F9A0EB3537D5E gbra1_human Gamma-aminobutyric acid receptor subunit alpha-1 GABRA1 GABA(A) receptor subunit alpha-1 protein protein human Homo sapiens Human MRKSPGLSDCLWAWILLLSTLTGRSYGQPSLQDELKDNTTVFTRILDRLLDGYDNRLRPGLGERVTEVKTDIFVTSFGPVSDHDMEYTIDVFFRQSWKDERLKFKGPMTVLRLNNLMASKIWTPDTFFHNGKKSVAHNMTMPNKLLRITEDGTLLYTMRLTVRAECPMHLEDFPMDAHACPLKFGSYAYTRAEVVYEWTREPARSVVVAEDGSRLNQYDLLGQTVDSGIVQSSTGEYVVMTTHFHLKRKIGYFVIQTYLPCIMTVILSQVSFWLNRESVPARTVFGVTTVLTMTTLSISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKRGYAWDGKSVVPEKPKKVKDPLIKKNNTYAPTATSYTPNLARGDPGLATIAKSATIEPKEVKPETKPPEPKKTFNSVSKIDRLSRIAFPLLFGIFNLVYWATYLNREPQLKAPTPHQ F81EC9ECBE64E94D cryo em-1 1 2 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified rho tag rho tag rho tag rho tag 1D4 tag rho1D4 tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293s Human 293S cell line Human 293s 293S cell line 3 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified human-293s Human 293S cell line Human 293s 293S cell line 4 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293s Human 293S cell line Human 293s 293S cell line direct interaction direct interaction Fig. 1: Structure of the α1β3γ2L GABAA receptor in complex with picrotoxin