DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Cryo-EM structure of the human α1β3γ2 GABAA receptor in a lipid bilayer. Cryo-EM structure of the human α1β3γ2 GABAA receptor in a lipid bilayer. Nature 2019 imex curation Laverty D., Desai R., Uchański T., Masiulis S., Stec WJ., Malinauskas T., Zivanov J., Pardon E., Steyaert J., Miller KW., Aricescu AR., Laverty D., Desai R., Uchański T., Masiulis S., Stec WJ., Malinauskas T., Zivanov J., Pardon E., Steyaert J., Miller KW., Aricescu AR. radu@mrc-lmb.cam.ac.uk 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Laverty D., Desai R., Uchański T., Masiulis S., Stec WJ., Malinauskas T., Zivanov J., Pardon E., Steyaert J., Miller KW., Aricescu AR., Laverty D., Desai R., Uchański T., Masiulis S., Stec WJ., Malinauskas T., Zivanov J., Pardon E., Steyaert J., Miller KW., Aricescu AR. radu@mrc-lmb.cam.ac.uk Accepted 2019-JUN-19 AT 12:15 BST AT 12:15 BST by ORCHARD Nature 2019 imex curation Host organism is the the point where the protein first interacted (interaction host) which in this case was HEK. Cryo-EM structure of the human α1β3γ2 GABAA receptor in a lipid bilayer. Cryo-EM structure of the human α1β3γ2 GABAA receptor in a lipid bilayer. Nature 2019 imex curation Laverty D., Desai R., Uchański T., Masiulis S., Stec WJ., Malinauskas T., Zivanov J., Pardon E., Steyaert J., Miller KW., Aricescu AR., Laverty D., Desai R., Uchański T., Masiulis S., Stec WJ., Malinauskas T., Zivanov J., Pardon E., Steyaert J., Miller KW., Aricescu AR. radu@mrc-lmb.cam.ac.uk 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Laverty D., Desai R., Uchański T., Masiulis S., Stec WJ., Malinauskas T., Zivanov J., Pardon E., Steyaert J., Miller KW., Aricescu AR., Laverty D., Desai R., Uchański T., Masiulis S., Stec WJ., Malinauskas T., Zivanov J., Pardon E., Steyaert J., Miller KW., Aricescu AR. The complex was purified from HEK293 cells, the EM was then performed in nanodiscs following a reconsitution step. radu@mrc-lmb.cam.ac.uk Accepted 2019-JUN-19 AT 12:15 BST AT 12:15 BST by ORCHARD Nature 2019 imex curation Host organism is the the point where the protein first interacted (interaction host) which in this case was HEK. p18507-2 Gamma-aminobutyric acid receptor subunit gamma-2 GABRG2 GABA(A) receptor subunit gamma-2 protein protein human Homo sapiens Human MSSPNIWSTGSSVYSTPVFSQKMTVWILLLLSLYPGFTSQKSDDDYEDYASNKTWVLTPKVPEGDVTVILNNLLEGYDNKLRPDIGVKPTLIHTDMYVNSIGPVNAINMEYTIDIFFAQTWYDRRLKFNSTIKVLRLNSNMVGKIWIPDTFFRNSKKADAHWITTPNRMLRIWNDGRVLYTLRLTIDAECQLQLHNFPMDEHSCPLEFSSYGYPREEIVYQWKRSSVEVGDTRSWRLYQFSFVGLRNTTEVVKTTSGDYVVMSVYFDLSRRMGYFTIQTYIPCTLIVVLSWVSFWINKDAVPARTSLGITTVLTMTTLSTIARKSLPKVSYVTAMDLFVSVCFIFVFSALVEYGTLHYFVSNRKPSKDKDKKKKNPLLRMFSFKAPTIDIRPRSATIQMNNATHLQERDEEYGYECLDGKDCASFFCCFEDCRTGAWRHGRIHIRIAKMDSYARIFFPTAFCLFNLVYWVSYLYL DE142F62846576DF p28472-2 Gamma-aminobutyric acid receptor subunit beta-3 GABA(A) receptor subunit beta-3 GABRB3 protein protein human Homo sapiens Human MCSGLLELLLPIWLSWTLGTRGSEPRSVNDPGNMSFVKETVDKLLKGYDIRLRPDFGGPPVCVGMNIDIASIDMVSEVNMDYTLTMYFQQYWRDKRLAYSGIPLNLTLDNRVADQLWVPDTYFLNDKKSFVHGVTVKNRMIRLHPDGTVLYGLRITTTAACMMDLRRYPLDEQNCTLEIESYGYTTDDIEFYWRGGDKAVTGVERIELPQFSIVEHRLVSRNVVFATGAYPRLSLSFRLKRNIGYFILQTYMPSILITILSWVSFWINYDASAARVALGITTVLTMTTINTHLRETLPKIPYVKAIDMYLMGCFVFVFLALLEYAFVNYIFFGRGPQRQKKLAEKTAKAKNDRSKSESNRVDAHGNILLTSLEVHNEMNEVSGGIGDTRNSAISFDNSGIQYRKQSMPREGHGRFLGDRSLPHKKTHLRRRSSQLKIKIPDLTDVNAIDRWSRIVFPFTFSLFNLVYWLYYVN Variant in position: 11:P->S (in ECA5, the mutant protein is hyperglycosylated and has reduced mean current densities compared to wild-type, dbSNP:rs25409). Variant in position: 15:S->F (in ECA5, the mutant protein is hyperglycosylated and has reduced mean current densities compared to wild-type). 3A7F9A0EB3537D5E gbra1_human Gamma-aminobutyric acid receptor subunit alpha-1 GABRA1 GABA(A) receptor subunit alpha-1 protein protein human Homo sapiens Human MRKSPGLSDCLWAWILLLSTLTGRSYGQPSLQDELKDNTTVFTRILDRLLDGYDNRLRPGLGERVTEVKTDIFVTSFGPVSDHDMEYTIDVFFRQSWKDERLKFKGPMTVLRLNNLMASKIWTPDTFFHNGKKSVAHNMTMPNKLLRITEDGTLLYTMRLTVRAECPMHLEDFPMDAHACPLKFGSYAYTRAEVVYEWTREPARSVVVAEDGSRLNQYDLLGQTVDSGIVQSSTGEYVVMTTHFHLKRKIGYFVIQTYLPCIMTVILSQVSFWLNRESVPARTVFGVTTVLTMTTLSISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKRGYAWDGKSVVPEKPKKVKDPLIKKNNTYAPTATSYTPNLARGDPGLATIAKSATIEPKEVKPETKPPEPKKTFNSVSKIDRLSRIAFPLLFGIFNLVYWATYLNREPQLKAPTPHQ F81EC9ECBE64E94D gabra1-gabrb3-2 2 3 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified rho tag 1D4 tag rho tag rho tag 1D4 tag rho1D4 tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 4 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 5 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association Fig. 1 gabra1-gabrb3-1 1 3 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait over-expressed over expressed level over-expressed purified purified rho tag 1D4 tag rho tag rho tag 1D4 tag rho1D4 tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 4 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey over-expressed over expressed level over-expressed purified purified human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 5 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey over-expressed over expressed level over-expressed purified purified n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. association association Extd Fig. 2C