DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis for GTP hydrolysis and conformational change of MFN1 in mediating membrane fusion. Structural basis for GTP hydrolysis and conformational change of MFN1 in mediating membrane fusion. Nature structural & molecular biology 2018 imex curation Yan L., Qi Y., Huang X., Yu C., Lan L., Guo X., Rao Z., Hu J., Lou Z. huj@ibp.ac.cn; louzy@mail.tsinghua.edu.cn 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Yan L., Qi Y., Huang X., Yu C., Lan L., Guo X., Rao Z., Hu J., Lou Z. huj@ibp.ac.cn; louzy@mail.tsinghua.edu.cn Accepted 2019-MAY-20 AT 13:06 BST AT 13:06 BST by ORCHARD Nature structural & molecular biology 2018 imex curation Structural basis for GTP hydrolysis and conformational change of MFN1 in mediating membrane fusion. Structural basis for GTP hydrolysis and conformational change of MFN1 in mediating membrane fusion. Nature structural & molecular biology 2018 imex curation Yan L., Qi Y., Huang X., Yu C., Lan L., Guo X., Rao Z., Hu J., Lou Z. huj@ibp.ac.cn; louzy@mail.tsinghua.edu.cn 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret structure determination identification by structure determination identification by structure determination structure determination Yan L., Qi Y., Huang X., Yu C., Lan L., Guo X., Rao Z., Hu J., Lou Z. huj@ibp.ac.cn; louzy@mail.tsinghua.edu.cn Accepted 2019-MAY-20 AT 13:06 BST AT 13:06 BST by ORCHARD Nature structural & molecular biology 2018 imex curation Structural basis for GTP hydrolysis and conformational change of MFN1 in mediating membrane fusion. Structural basis for GTP hydrolysis and conformational change of MFN1 in mediating membrane fusion. Nature structural & molecular biology 2018 imex curation Yan L., Qi Y., Huang X., Yu C., Lan L., Guo X., Rao Z., Hu J., Lou Z. huj@ibp.ac.cn; louzy@mail.tsinghua.edu.cn 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction experimental particp experimental participant identification experimental particp Yan L., Qi Y., Huang X., Yu C., Lan L., Guo X., Rao Z., Hu J., Lou Z. huj@ibp.ac.cn; louzy@mail.tsinghua.edu.cn Accepted 2019-MAY-20 AT 13:06 BST AT 13:06 BST by ORCHARD Nature structural & molecular biology 2018 imex curation mfn1_human Mitofusin-1 Transmembrane GTPase MFN1 Fzo homolog MFN1 protein protein human Homo sapiens Human MAEPVSPLKHFVLAKKAITAIFDQLLEFVTEGSHFVEATYKNPELDRIATEDDLVEMQGYKDKLSIIGEVLSRRHMKVAFFGRTSSGKSSVINAMLWDKVLPSGIGHITNCFLSVEGTDGDKAYLMTEGSDEKKSVKTVNQLAHALHMDKDLKAGCLVRVFWPKAKCALLRDDLVLVDSPGTDVTTELDSWIDKFCLDADVFVLVANSESTLMNTEKHFFHKVNERLSKPNIFILNNRWDASASEPEYMEDVRRQHMERCLHFLVEELKVVNALEAQNRIFFVSAKEVLSARKQKAQGMPESGVALAEGFHARLQEFQNFEQIFEECISQSAVKTKFEQHTIRAKQILATVKNIMDSVNLAAEDKRHYSVEEREDQIDRLDFIRNQMNLLTLDVKKKIKEVTEEVANKVSCAMTDEICRLSVLVDEFCSEFHPNPDVLKIYKSELNKHIEDGMGRNLADRCTDEVNALVLQTQQEIIENLKPLLPAGIQDKLHTLIPCKKFDLSYNLNYHKLCSDFQEDIVFRFSLGWSSLVHRFLGPRNAQRVLLGLSEPIFQLPRSLASTPTAPTTPATPDNASQEELMITLVTGLASVTSRTSMGIIIVGGVIWKTIGWKLLSVSLTMYGALYLYERLSWTTHAKERAFKQQFVNYATEKLRMIVSSTSANCSHQVKQQIATTFARLCQQVDITQKQLEEEIARLPKEIDQLEKIQNNSKLLRNKAVQLENELENFTKQFLPSSNEES DIP protein Q8IWA4 original sequence version: 106 DIP protein Q8IWA4-1 original sequence version: 52 B7BDC22437E30A58 mfn1-1 3 4 structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain KRHYSVEEREDQIDRLDFIRNQMNLLTLDVKKKIKEVTEEVANKVSCAMTDEICRLSVLVDEFCSEFHPNPDVLKIYKSELNKHIEDGMGRNLADRCTDEVNALVLQTQQEIIENLKPLLPAGIQDKLHTLIPCKKFDLSYNLNYHKLCSDFQEDIVFRFSLGWSSLVHRFLGPRNAQRVLLGLSEPIFQLPRSLASTPTAPTTPATPDNASQEELMITLVTGLASVTSRTSMGIIIVGGVIWKTIGWKLLSVSLTMYGALYLYERLSWTTHAKERAFKQQFVNYATEKLRMIVSSTSANCSHQVKQQIATTFARLCQQVDITQKQLEE GSGSGSGGS ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1, Table 1 mfn1-15 2 4 structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role fluorescence donor fluorescence donor purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain KRHYSVEEREDQIDRLDFIRNQMNLLTLDVKKKIKEVTEEVANKVSCAMTDEICRLSVLVDEFCSEFHPNPDVLKIYKSELNKHIEDGMGRNLADRCTDEVNALVLQTQQEIIENLKPLLPAGIQDKLHTLIPCKKFDLSYNLNYHKLCSDFQEDIVFRFSLGWSSLVHRFLGPRNAQRVLLGLSEPIFQLPRSLASTPTAPTTPATPDNASQEELMITLVTGLASVTSRTSMGIIIVGGVIWKTIGWKLLSVSLTMYGALYLYERLSWTTHAKERAFKQQFVNYATEKLRMIVSSTSANCSHQVKQQIATTFARLCQQVDITQKQLEE GSGSGSGGS cy5 label cy5 label cy5 label cy5 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 4 structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain KRHYSVEEREDQIDRLDFIRNQMNLLTLDVKKKIKEVTEEVANKVSCAMTDEICRLSVLVDEFCSEFHPNPDVLKIYKSELNKHIEDGMGRNLADRCTDEVNALVLQTQQEIIENLKPLLPAGIQDKLHTLIPCKKFDLSYNLNYHKLCSDFQEDIVFRFSLGWSSLVHRFLGPRNAQRVLLGLSEPIFQLPRSLASTPTAPTTPATPDNASQEELMITLVTGLASVTSRTSMGIIIVGGVIWKTIGWKLLSVSLTMYGALYLYERLSWTTHAKERAFKQQFVNYATEKLRMIVSSTSANCSHQVKQQIATTFARLCQQVDITQKQLEE GSGSGSGGS cy3 label cy3 label cy3 label cy3 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 6, Supplementary Figure 6 mfn1-10 1 4 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain KRHYSVEEREDQIDRLDFIRNQMNLLTLDVKKKIKEVTEEVANKVSCAMTDEICRLSVLVDEFCSEFHPNPDVLKIYKSELNKHIEDGMGRNLADRCTDEVNALVLQTQQEIIENLKPLLPAGIQDKLHTLIPCKKFDLSYNLNYHKLCSDFQEDIVFRFSLGWSSLVHRFLGPRNAQRVLLGLSEPIFQLPRSLASTPTAPTTPATPDNASQEELMITLVTGLASVTSRTSMGIIIVGGVIWKTIGWKLLSVSLTMYGALYLYERLSWTTHAKERAFKQQFVNYATEKLRMIVSSTSANCSHQVKQQIATTFARLCQQVDITQKQLEE GSGSGSGGS ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 3b, Supplementary Fig.4c mfn1-16 1 4 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain KRHYSVEEREDQIDRLDFIRNQMNLLTLDVKKKIKEVTEEVANKVSCAMTDEICRLSVLVDEFCSEFHPNPDVLKIYKSELNKHIEDGMGRNLADRCTDEVNALVLQTQQEIIENLKPLLPAGIQDKLHTLIPCKKFDLSYNLNYHKLCSDFQEDIVFRFSLGWSSLVHRFLGPRNAQRVLLGLSEPIFQLPRSLASTPTAPTTPATPDNASQEELMITLVTGLASVTSRTSMGIIIVGGVIWKTIGWKLLSVSLTMYGALYLYERLSWTTHAKERAFKQQFVNYATEKLRMIVSSTSANCSHQVKQQIATTFARLCQQVDITQKQLEE GSGSGSGGS Q8IWA4:p.Arg238Ala R238A mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain R A Tue Jun 30 17:33:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg238Ala' Q8IWA4:p.Ser85Ala S85A mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 18:56:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser85Ala' ecoli Escherichia coli (strain K12) direct interaction direct interaction Supplementary Fig. 4e and f mfn1-17 1 4 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain KRHYSVEEREDQIDRLDFIRNQMNLLTLDVKKKIKEVTEEVANKVSCAMTDEICRLSVLVDEFCSEFHPNPDVLKIYKSELNKHIEDGMGRNLADRCTDEVNALVLQTQQEIIENLKPLLPAGIQDKLHTLIPCKKFDLSYNLNYHKLCSDFQEDIVFRFSLGWSSLVHRFLGPRNAQRVLLGLSEPIFQLPRSLASTPTAPTTPATPDNASQEELMITLVTGLASVTSRTSMGIIIVGGVIWKTIGWKLLSVSLTMYGALYLYERLSWTTHAKERAFKQQFVNYATEKLRMIVSSTSANCSHQVKQQIATTFARLCQQVDITQKQLEE GSGSGSGGS his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q8IWA4:p.Glu209Ala E209A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain E A Tue Jun 30 18:43:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu209Ala' Q8IWA4:p.His147Ala H147A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain H A Tue Jun 30 19:07:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His147Ala' Q8IWA4:p.Lys150Ala R150A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 19:47:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys150Ala' Q8IWA4:p.Glu245Ala E245A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain E A Tue Jun 30 19:46:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu245Ala' Q8IWA4:p.Tyr248Ala Y248A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain Y A Tue Jun 30 15:58:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr248Ala' Q8IWA4:p.Ile105Ala I105A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain I A Tue Jun 30 18:47:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile105Ala' ecoli Escherichia coli (strain K12) direct interaction direct interaction Supplementary Fig. 4e mfn1-18 1 4 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain KRHYSVEEREDQIDRLDFIRNQMNLLTLDVKKKIKEVTEEVANKVSCAMTDEICRLSVLVDEFCSEFHPNPDVLKIYKSELNKHIEDGMGRNLADRCTDEVNALVLQTQQEIIENLKPLLPAGIQDKLHTLIPCKKFDLSYNLNYHKLCSDFQEDIVFRFSLGWSSLVHRFLGPRNAQRVLLGLSEPIFQLPRSLASTPTAPTTPATPDNASQEELMITLVTGLASVTSRTSMGIIIVGGVIWKTIGWKLLSVSLTMYGALYLYERLSWTTHAKERAFKQQFVNYATEKLRMIVSSTSANCSHQVKQQIATTFARLCQQVDITQKQLEE GSGSGSGGS Q8IWA4:p.Gly106Asp G106D mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain G D Tue Jun 30 18:12:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly106Asp' Q8IWA4:p.Gln255Arg Q255R mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain Q R Wed Sep 18 12:58:21 BST 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu255Arg' Tue Jun 30 16:41:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln255Arg' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig .5b mfn1-19 1 4 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain KRHYSVEEREDQIDRLDFIRNQMNLLTLDVKKKIKEVTEEVANKVSCAMTDEICRLSVLVDEFCSEFHPNPDVLKIYKSELNKHIEDGMGRNLADRCTDEVNALVLQTQQEIIENLKPLLPAGIQDKLHTLIPCKKFDLSYNLNYHKLCSDFQEDIVFRFSLGWSSLVHRFLGPRNAQRVLLGLSEPIFQLPRSLASTPTAPTTPATPDNASQEELMITLVTGLASVTSRTSMGIIIVGGVIWKTIGWKLLSVSLTMYGALYLYERLSWTTHAKERAFKQQFVNYATEKLRMIVSSTSANCSHQVKQQIATTFARLCQQVDITQKQLEE GSGSGSGGS Q8IWA4:p.Lys229Gln K229Q mutation mutation certain certain sequence position certain certain certain sequence position certain K Q Tue Jun 30 15:43:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys229Gln' Q8IWA4:p.Asp198Asn D198N mutation mutation certain certain sequence position certain certain certain sequence position certain D N Tue Jun 30 16:39:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp198Asn' Q8IWA4:p.Lys229Trp K229W mutation mutation certain certain sequence position certain certain certain sequence position certain K W Tue Jun 30 15:42:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys229Trp' Q8IWA4:p.Arg259His R259H mutation mutation certain certain sequence position certain certain certain sequence position certain R H Tue Jun 30 18:59:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg259His' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig .5b and c mfn1-20 1 4 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain KRHYSVEEREDQIDRLDFIRNQMNLLTLDVKKKIKEVTEEVANKVSCAMTDEICRLSVLVDEFCSEFHPNPDVLKIYKSELNKHIEDGMGRNLADRCTDEVNALVLQTQQEIIENLKPLLPAGIQDKLHTLIPCKKFDLSYNLNYHKLCSDFQEDIVFRFSLGWSSLVHRFLGPRNAQRVLLGLSEPIFQLPRSLASTPTAPTTPATPDNASQEELMITLVTGLASVTSRTSMGIIIVGGVIWKTIGWKLLSVSLTMYGALYLYERLSWTTHAKERAFKQQFVNYATEKLRMIVSSTSANCSHQVKQQIATTFARLCQQVDITQKQLEE GSGSGSGGS Q8IWA4:p.[Arg73Gln;Asp198Asn] R73Q,D198N mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R Q certain certain sequence position certain certain certain sequence position certain D N Tue Jun 30 20:22:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg73Gln;Asp198Asn]' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 5c mfn1-21 1 4 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain KRHYSVEEREDQIDRLDFIRNQMNLLTLDVKKKIKEVTEEVANKVSCAMTDEICRLSVLVDEFCSEFHPNPDVLKIYKSELNKHIEDGMGRNLADRCTDEVNALVLQTQQEIIENLKPLLPAGIQDKLHTLIPCKKFDLSYNLNYHKLCSDFQEDIVFRFSLGWSSLVHRFLGPRNAQRVLLGLSEPIFQLPRSLASTPTAPTTPATPDNASQEELMITLVTGLASVTSRTSMGIIIVGGVIWKTIGWKLLSVSLTMYGALYLYERLSWTTHAKERAFKQQFVNYATEKLRMIVSSTSANCSHQVKQQIATTFARLCQQVDITQKQLEE GSGSGSGGS Q8IWA4:p.Ser86Ala S86A mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 17:55:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser86Ala' ecoli Escherichia coli (strain K12) direct interaction direct interaction Supplementary Fig. 4F mfn1-22 1 4 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain KRHYSVEEREDQIDRLDFIRNQMNLLTLDVKKKIKEVTEEVANKVSCAMTDEICRLSVLVDEFCSEFHPNPDVLKIYKSELNKHIEDGMGRNLADRCTDEVNALVLQTQQEIIENLKPLLPAGIQDKLHTLIPCKKFDLSYNLNYHKLCSDFQEDIVFRFSLGWSSLVHRFLGPRNAQRVLLGLSEPIFQLPRSLASTPTAPTTPATPDNASQEELMITLVTGLASVTSRTSMGIIIVGGVIWKTIGWKLLSVSLTMYGALYLYERLSWTTHAKERAFKQQFVNYATEKLRMIVSSTSANCSHQVKQQIATTFARLCQQVDITQKQLEE GSGSGSGGS Q8IWA4:p.Arg73Asp R73D mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain R D Tue Jun 30 16:13:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg73Asp' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 4c mfn1-23 1 4 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain KRHYSVEEREDQIDRLDFIRNQMNLLTLDVKKKIKEVTEEVANKVSCAMTDEICRLSVLVDEFCSEFHPNPDVLKIYKSELNKHIEDGMGRNLADRCTDEVNALVLQTQQEIIENLKPLLPAGIQDKLHTLIPCKKFDLSYNLNYHKLCSDFQEDIVFRFSLGWSSLVHRFLGPRNAQRVLLGLSEPIFQLPRSLASTPTAPTTPATPDNASQEELMITLVTGLASVTSRTSMGIIIVGGVIWKTIGWKLLSVSLTMYGALYLYERLSWTTHAKERAFKQQFVNYATEKLRMIVSSTSANCSHQVKQQIATTFARLCQQVDITQKQLEE GSGSGSGGS Q8IWA4:p.Asp198Arg D198R mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain D R Tue Jun 30 19:08:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp198Arg' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 4c mfn1-24 1 4 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain KRHYSVEEREDQIDRLDFIRNQMNLLTLDVKKKIKEVTEEVANKVSCAMTDEICRLSVLVDEFCSEFHPNPDVLKIYKSELNKHIEDGMGRNLADRCTDEVNALVLQTQQEIIENLKPLLPAGIQDKLHTLIPCKKFDLSYNLNYHKLCSDFQEDIVFRFSLGWSSLVHRFLGPRNAQRVLLGLSEPIFQLPRSLASTPTAPTTPATPDNASQEELMITLVTGLASVTSRTSMGIIIVGGVIWKTIGWKLLSVSLTMYGALYLYERLSWTTHAKERAFKQQFVNYATEKLRMIVSSTSANCSHQVKQQIATTFARLCQQVDITQKQLEE GSGSGSGGS Q8IWA4:p.[Arg73Asp;Asp198Arg] R73D,D198R mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain D R certain certain sequence position certain certain certain sequence position certain R D Tue Jun 30 20:07:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg73Asp;Asp198Arg]' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 4c mfn1-25 1 4 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain KRHYSVEEREDQIDRLDFIRNQMNLLTLDVKKKIKEVTEEVANKVSCAMTDEICRLSVLVDEFCSEFHPNPDVLKIYKSELNKHIEDGMGRNLADRCTDEVNALVLQTQQEIIENLKPLLPAGIQDKLHTLIPCKKFDLSYNLNYHKLCSDFQEDIVFRFSLGWSSLVHRFLGPRNAQRVLLGLSEPIFQLPRSLASTPTAPTTPATPDNASQEELMITLVTGLASVTSRTSMGIIIVGGVIWKTIGWKLLSVSLTMYGALYLYERLSWTTHAKERAFKQQFVNYATEKLRMIVSSTSANCSHQVKQQIATTFARLCQQVDITQKQLEE GSGSGSGGS Q8IWA4:p.Arg73Gln R73Q mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R Q Tue Jun 30 17:34:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg73Gln' Q8IWA4:p.Val252Gly V252G mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain V G Tue Jun 30 16:51:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val252Gly' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 5b and c