UniProt UniProt http://www.uniprot.org Structural insight into the E. coli HigBA complex. Structural insight into the E. coli HigBA complex. Biochemical and biophysical research communications 2016 imex curation Yang J., Zhou K., Liu P., Dong Y., Gao Z., Zhang J., Liu Q. liuqsh@ihep.ac.cn 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa weight by bromide molecular weight estimation by bromide staining weight by bromide Yang J., Zhou K., Liu P., Dong Y., Gao Z., Zhang J., Liu Q. liuqsh@ihep.ac.cn Accepted 2020-JAN-16 AT 11:01 GMT AT 11:01 GMT by LUANA Biochemical and biophysical research communications 2016 Only protein-protein interactions imex curation imex curation Structural insight into the E. coli HigBA complex. Structural insight into the E. coli HigBA complex. Biochemical and biophysical research communications 2016 imex curation Yang J., Zhou K., Liu P., Dong Y., Gao Z., Zhang J., Liu Q. liuqsh@ihep.ac.cn 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Yang J., Zhou K., Liu P., Dong Y., Gao Z., Zhang J., Liu Q. liuqsh@ihep.ac.cn Accepted 2020-JAN-16 AT 11:01 GMT AT 11:01 GMT by LUANA Biochemical and biophysical research communications 2016 Only protein-protein interactions imex curation imex curation Structural insight into the E. coli HigBA complex. Structural insight into the E. coli HigBA complex. Biochemical and biophysical research communications 2016 imex curation Yang J., Zhou K., Liu P., Dong Y., Gao Z., Zhang J., Liu Q. liuqsh@ihep.ac.cn 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Yang J., Zhou K., Liu P., Dong Y., Gao Z., Zhang J., Liu Q. liuqsh@ihep.ac.cn Accepted 2020-JAN-16 AT 11:01 GMT AT 11:01 GMT by LUANA Biochemical and biophysical research communications 2016 Only protein-protein interactions imex curation imex curation higb_ecoli mRNA interferase toxin HigB higB b3083 JW3054 ygjN Endoribonuclease HigB Toxin HigB protein protein ecoli Escherichia coli (strain K12) MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKGKK F23FB5F433C52C22 higa_ecoli Antitoxin HigA higA b3082 JW3053 ygjM protein protein ecoli Escherichia coli (strain K12) MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID 4A14D9E43480DCB5 higb_ecoli_gene higB gene gene gene ecoli Escherichia coli (strain K12) higa-higb-1 2 5 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1 higa-higb-2 3 5 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1 higa-higb-3 3 5 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1 higa-higb-4 1 6 unspecified role unspecified role bait bait promoter region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 5 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association Fig 3, 4 higb_ecoli_gene-higa-1 1 5 unspecified role unspecified role prey prey purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait promoter region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Fig. 4