IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Complement Factor H Binds to Human Serum Apolipoprotein E and Mediates Complement Regulation on High Density Lipoprotein Particles. Complement Factor H Binds to Human Serum Apolipoprotein E and Mediates Complement Regulation on High Density Lipoprotein Particles. The Journal of biological chemistry 2015 imex curation Haapasalo K., van Kessel K., Nissilä E., Metso J., Johansson T., Miettinen S., Varjosalo M., Kirveskari J., Kuusela P., Chroni A., Jauhiainen M., van Strijp J., Jokiranta TS. K.J.Haapasalo-Tuomainen@umcutrecht.nl 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro solid phase assay solid phase assay tag visualisation tag visualisation Haapasalo K., van Kessel K., Nissilä E., Metso J., Johansson T., Miettinen S., Varjosalo M., Kirveskari J., Kuusela P., Chroni A., Jauhiainen M., van Strijp J., Jokiranta TS. K.J.Haapasalo-Tuomainen@umcutrecht.nl Accepted 2019-DEC-09 AT 16:54 GMT AT 16:54 GMT by PPORRAS The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation Complement Factor H Binds to Human Serum Apolipoprotein E and Mediates Complement Regulation on High Density Lipoprotein Particles. Complement Factor H Binds to Human Serum Apolipoprotein E and Mediates Complement Regulation on High Density Lipoprotein Particles. The Journal of biological chemistry 2015 imex curation Haapasalo K., van Kessel K., Nissilä E., Metso J., Johansson T., Miettinen S., Varjosalo M., Kirveskari J., Kuusela P., Chroni A., Jauhiainen M., van Strijp J., Jokiranta TS. K.J.Haapasalo-Tuomainen@umcutrecht.nl 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Haapasalo K., van Kessel K., Nissilä E., Metso J., Johansson T., Miettinen S., Varjosalo M., Kirveskari J., Kuusela P., Chroni A., Jauhiainen M., van Strijp J., Jokiranta TS. K.J.Haapasalo-Tuomainen@umcutrecht.nl Accepted 2019-DEC-09 AT 16:54 GMT AT 16:54 GMT by PPORRAS The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation Complement Factor H Binds to Human Serum Apolipoprotein E and Mediates Complement Regulation on High Density Lipoprotein Particles. Complement Factor H Binds to Human Serum Apolipoprotein E and Mediates Complement Regulation on High Density Lipoprotein Particles. The Journal of biological chemistry 2015 imex curation Haapasalo K., van Kessel K., Nissilä E., Metso J., Johansson T., Miettinen S., Varjosalo M., Kirveskari J., Kuusela P., Chroni A., Jauhiainen M., van Strijp J., Jokiranta TS. K.J.Haapasalo-Tuomainen@umcutrecht.nl 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down ms participant Identification by mass spectrometry ms participant Haapasalo K., van Kessel K., Nissilä E., Metso J., Johansson T., Miettinen S., Varjosalo M., Kirveskari J., Kuusela P., Chroni A., Jauhiainen M., van Strijp J., Jokiranta TS. amino acid boundaries of FH SCR domains taken from Ref. 16 K.J.Haapasalo-Tuomainen@umcutrecht.nl Accepted 2019-DEC-09 AT 16:54 GMT AT 16:54 GMT by PPORRAS The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation Complement Factor H Binds to Human Serum Apolipoprotein E and Mediates Complement Regulation on High Density Lipoprotein Particles. Complement Factor H Binds to Human Serum Apolipoprotein E and Mediates Complement Regulation on High Density Lipoprotein Particles. The Journal of biological chemistry 2015 imex curation Haapasalo K., van Kessel K., Nissilä E., Metso J., Johansson T., Miettinen S., Varjosalo M., Kirveskari J., Kuusela P., Chroni A., Jauhiainen M., van Strijp J., Jokiranta TS. K.J.Haapasalo-Tuomainen@umcutrecht.nl 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro mst microscale thermophoresis mst predetermined predetermined participant predetermined Haapasalo K., van Kessel K., Nissilä E., Metso J., Johansson T., Miettinen S., Varjosalo M., Kirveskari J., Kuusela P., Chroni A., Jauhiainen M., van Strijp J., Jokiranta TS. K.J.Haapasalo-Tuomainen@umcutrecht.nl Accepted 2019-DEC-09 AT 16:54 GMT AT 16:54 GMT by PPORRAS The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation cfah_human Complement factor H CFH HF HF1 HF2 H factor 1 protein protein human Homo sapiens Human MRLLAKIICLMLWAICVAEDCNELPPRRNTEILTGSWSDQTYPEGTQAIYKCRPGYRSLGNVIMVCRKGEWVALNPLRKCQKRPCGHPGDTPFGTFTLTGGNVFEYGVKAVYTCNEGYQLLGEINYRECDTDGWTNDIPICEVVKCLPVTAPENGKIVSSAMEPDREYHFGQAVRFVCNSGYKIEGDEEMHCSDDGFWSKEKPKCVEISCKSPDVINGSPISQKIIYKENERFQYKCNMGYEYSERGDAVCTESGWRPLPSCEEKSCDNPYIPNGDYSPLRIKHRTGDEITYQCRNGFYPATRGNTAKCTSTGWIPAPRCTLKPCDYPDIKHGGLYHENMRRPYFPVAVGKYYSYYCDEHFETPSGSYWDHIHCTQDGWSPAVPCLRKCYFPYLENGYNQNYGRKFVQGKSIDVACHPGYALPKAQTTVTCMENGWSPTPRCIRVKTCSKSSIDIENGFISESQYTYALKEKAKYQCKLGYVTADGETSGSITCGKDGWSAQPTCIKSCDIPVFMNARTKNDFTWFKLNDTLDYECHDGYESNTGSTTGSIVCGYNGWSDLPICYERECELPKIDVHLVPDRKKDQYKVGEVLKFSCKPGFTIVGPNSVQCYHFGLSPDLPICKEQVQSCGPPPELLNGNVKEKTKEEYGHSEVVEYYCNPRFLMKGPNKIQCVDGEWTTLPVCIVEESTCGDIPELEHGWAQLSSPPYYYGDSVEFNCSESFTMIGHRSITCIHGVWTQLPQCVAIDKLKKCKSSNLIILEEHLKNKKEFDHNSNIRYRCRGKEGWIHTVCINGRWDPEVNCSMAQIQLCPPPPQIPNSHNMTTTLNYRDGEKVSVLCQENYLIQEGEEITCKDGRWQSIPLCVEKIPCSQPPQIEHGTINSSRSSQESYAHGTKLSYTCEGGFRISEENETTCYMGKWSSPPQCEGLPCKSPPEISHGVVAHMSDSYQYGEEVTYKCFEGFGIDGPAIAKCLGEKWSHPPSCIKTDCLSLPSFENAIPMGEKKDVYKAGEQVTYTCATYYKMDGASNVTCINSRWTGRPTCRDTSCVNPPTVQNAYIVSRQMSKYPSGERVRYQCRSPYEMFGDEEVMCLNGNWTEPPQCKDSTGKCGPPPPIDNGDITSFPLSVYAPASSVEYQCQNLYQLEGNKRITCRNGQWSEPPKCLHPCVISREIMENYNIALRWTAKQKLYSRTGESVEFVCKRGYRLSSRSHTLRTTCWDGKLEYPTCAKR DIP protein P08603 original sequence version: 161 DIP protein P08603-1 original sequence version: 149 3C26D62A2BF9BFEE apoe_human Apolipoprotein E APOE protein protein human Homo sapiens Human MKVLWAALLVTFLAGCQAKVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH DIP protein P02649 original sequence version: 197 DIP protein P02649 original sequence version: 146 91AFC04210A30689 cfh-apoe-1 3 6 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-serum Homo sapiens serum Serum 5 unspecified role unspecified role bait bait purified purified SCR/Sushi_domains 5-7 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined picpa Pichia pastoris (Yeast) Yeast Pichia pastoris physical association physical association 5A, Table 1 This is probably the APOE*3 variant. cfh-apoe-2 4 6 unspecified role unspecified role neutral component neutral component purified purified red_dye dye labelled dye label dye labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component purified purified SCR/Sushi_domains 5-7 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction 5B The APOE*3 variant . apoe-cfh-3 1 6 western blot western blot Immuno blot Immuno blot tag visualisation tag visualisation unspecified role unspecified role prey prey purified purified region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 5 predetermined predetermined participant predetermined tag visualisation tag visualisation unspecified role unspecified role bait bait purified purified human-serum Homo sapiens serum Serum direct interaction direct interaction 7A/C The APOE*3 variant . apoe-cfh-5 1 5 unspecified role unspecified role bait bait purified purified human-serum Homo sapiens serum Serum 6 western blot western blot Immuno blot Immuno blot tag visualisation tag visualisation unspecified role unspecified role prey prey purified purified region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 5 predetermined predetermined participant predetermined tag visualisation tag visualisation competitor competitor competitor unspecified role unspecified role purified purified region fitc labelled fluorescein isothiocyanate label fluorescein isothiocyanate labbeled FITC labelled FITC labelled fluorescein isothiocyanate labbeled fitc labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined SCR/Sushi_domains 5-7 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain picpa Pichia pastoris (Yeast) Yeast Pichia pastoris physical association physical association 7C-2 The APOE*3 variant . cfh-apoe-3 1 6 competitor competitor competitor unspecified role unspecified role purified purified ecoli Escherichia coli (strain K12) 6 western blot western blot Immuno blot Immuno blot tag visualisation tag visualisation unspecified role unspecified role prey prey purified purified region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 5 predetermined predetermined participant predetermined tag visualisation tag visualisation unspecified role unspecified role bait bait purified purified human-serum Homo sapiens serum Serum physical association physical association 7A-2 The APOE*3 variant . cfh-apoe-4 1 6 western blot western blot Immuno blot Immuno blot tag visualisation tag visualisation unspecified role unspecified role prey prey purified purified region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 5 predetermined predetermined participant predetermined tag visualisation tag visualisation unspecified role unspecified role bait bait purified purified region fitc labelled fluorescein isothiocyanate label fluorescein isothiocyanate labbeled FITC labelled FITC labelled fluorescein isothiocyanate labbeled fitc labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined SCR/Sushi_domains 5-7 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction 7B-1 The APOE*3 variant . cfh-apoe-5 1 6 western blot western blot Immuno blot Immuno blot tag visualisation tag visualisation competitor competitor competitor unspecified role unspecified role purified purified ecoli Escherichia coli (strain K12) 5 predetermined predetermined participant predetermined tag visualisation tag visualisation unspecified role unspecified role bait bait purified purified region fitc labelled fluorescein isothiocyanate label fluorescein isothiocyanate labbeled FITC labelled FITC labelled fluorescein isothiocyanate labbeled fitc labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined SCR/Sushi_domains 5-7 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain picpa Pichia pastoris (Yeast) Yeast Pichia pastoris 6 western blot western blot Immuno blot Immuno blot tag visualisation tag visualisation unspecified role unspecified role prey prey purified purified region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 7B-2 The APOE*3 variant . cfh-apoe-6 2 6 western blot western blot Immuno blot Immuno blot antibody detection identification by antibody antibody detection unspecified role unspecified role bait bait purified purified P02649:p.Cys130Arg mutation mutation certain certain sequence position certain certain certain sequence position certain C R The APOE*4 variant :P02649 VAR_000652. Tue Jun 30 18:06:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys130Arg' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 predetermined predetermined participant predetermined antibody detection identification by antibody antibody detection unspecified role unspecified role prey prey purified purified region fitc labelled fluorescein isothiocyanate label fluorescein isothiocyanate labbeled FITC labelled FITC labelled fluorescein isothiocyanate labbeled fitc labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined SCR/Sushi_domains 5-7 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction 7D The APOE*4 variant :P02649 VAR_000652.