IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Mapping Key Residues of ISD11 Critical for NFS1-ISD11 Subcomplex Stability: IMPLICATIONS IN THE DEVELOPMENT OF MITOCHONDRIAL DISORDER, COXPD19. Mapping Key Residues of ISD11 Critical for NFS1-ISD11 Subcomplex Stability: IMPLICATIONS IN THE DEVELOPMENT OF MITOCHONDRIAL DISORDER, COXPD19. The Journal of biological chemistry 2015 imex curation Saha PP., Srivastava S., Kumar S K P., Sinha D., D'Silva P. patrick@biochem.iisc.ernet.in 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Saha PP., Srivastava S., Kumar S K P., Sinha D., D'Silva P. patrick@biochem.iisc.ernet.in Accepted 2019-SEP-19 AT 08:37 BST AT 08:37 BST by PPORRAS The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation Mapping Key Residues of ISD11 Critical for NFS1-ISD11 Subcomplex Stability: IMPLICATIONS IN THE DEVELOPMENT OF MITOCHONDRIAL DISORDER, COXPD19. Mapping Key Residues of ISD11 Critical for NFS1-ISD11 Subcomplex Stability: IMPLICATIONS IN THE DEVELOPMENT OF MITOCHONDRIAL DISORDER, COXPD19. The Journal of biological chemistry 2015 imex curation Saha PP., Srivastava S., Kumar S K P., Sinha D., D'Silva P. patrick@biochem.iisc.ernet.in 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Saha PP., Srivastava S., Kumar S K P., Sinha D., D'Silva P. GST tag of LYRM4 construct was used for pull down patrick@biochem.iisc.ernet.in Accepted 2019-SEP-19 AT 08:37 BST AT 08:37 BST by PPORRAS The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation Mapping Key Residues of ISD11 Critical for NFS1-ISD11 Subcomplex Stability: IMPLICATIONS IN THE DEVELOPMENT OF MITOCHONDRIAL DISORDER, COXPD19. Mapping Key Residues of ISD11 Critical for NFS1-ISD11 Subcomplex Stability: IMPLICATIONS IN THE DEVELOPMENT OF MITOCHONDRIAL DISORDER, COXPD19. The Journal of biological chemistry 2015 imex curation Saha PP., Srivastava S., Kumar S K P., Sinha D., D'Silva P. patrick@biochem.iisc.ernet.in 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro light scattering light scattering predetermined predetermined participant predetermined Saha PP., Srivastava S., Kumar S K P., Sinha D., D'Silva P. Light scattering is employed to monitor prevention of NFS1 aggregation (as a measure of Fe-S cluster biogenesis impairment) patrick@biochem.iisc.ernet.in Accepted 2019-SEP-19 AT 08:37 BST AT 08:37 BST by PPORRAS The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation lyrm4_human LYR motif-containing protein 4 LYRM4 C6orf149 ISD11 CGI-203 protein protein human Homo sapiens Human MAASSRAQVLSLYRAMLRESKRFSAYNYRTYAVRRIRDAFRENKNVKDPVEIQTLVNKAKRDLGVIRRQVHIGQLYSTDKLIIENRDMPRT F0C5919CA701F3F3 nfs1_human Cysteine desulfurase, mitochondrial NFS1 NIFS HUSSY-08 protein protein human Homo sapiens Human MLLRAAWRRAAVAVTAAPGPKPAAPTRGLRLRVGDRAPQSAVPADTAAAPEVGPVLRPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGADPREIIFTSGATESNNIAIKGVARFYRSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYLPVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGRICSSRKVYFHTDAAQAVGKIPLDVNDMKIDLMSISGHKIYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTVPTPLVVGLGAACEVAQQEMEYDHKRISKLSERLIQNIMKSLPDVVMNGDPKHHYPGCINLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTEEEVDYTVEKCIQHVKRLREMSPLWEMVQDGIDLKSIKWTQH FDE76177DB6E751B lyrm4-nfs1-1 2 5 unspecified role unspecified role prey prey purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9HD34:p.Ile72Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I A Tue Jun 30 19:21:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile72Ala' Q9HD34:p.Phe40Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F A Tue Jun 30 17:46:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe40Ala' Q9HD34:p.Glu84Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E A Tue Jun 30 16:42:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu84Ala' Q9HD34:p.Arg68Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Tue Jun 30 20:14:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg68Ala' Q9HD34:p.Gln69Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Q A Tue Jun 30 19:20:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln69Ala' Q9HD34:p.Tyr76Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y A Thu Jul 11 17:52:58 BST 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr76Ala' Tue Jun 30 19:28:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr76Ala' Q9HD34:p.Leu81Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L A Tue Jun 30 16:44:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu81Ala' Q9HD34:p.Leu63Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L A Tue Jun 30 17:42:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu63Ala' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 3a-3p lyrm4-nfs1-2 1 5 unspecified role unspecified role bait bait purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep Q9HD34:p.Gln69Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Q A Tue Jun 30 20:05:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln69Ala' Q9HD34:p.Arg68Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Tue Jun 30 18:12:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg68Ala' Q9HD34:p.Leu63Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L A Tue Jun 30 15:43:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu63Ala' Q9HD34:p.Tyr76Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y A Thu Jul 11 17:32:57 BST 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr76Ala' Tue Jun 30 19:10:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr76Ala' Q9HD34:p.Leu81Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L A Tue Jun 30 17:24:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu81Ala' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeast Saccharomyces cerevisiae Baker's yeast physical association physical association Figure 3q, 3r lyrm4-nfs1-3 3 5 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component purified purified Q9HD34:p.Leu81Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L A Tue Jun 30 18:27:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu81Ala' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9HD34:p.Phe40Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F A Tue Jun 30 15:41:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe40Ala' Q9HD34:p.Leu63Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L A Tue Jun 30 17:18:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu63Ala' Q9HD34:p.Arg68Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R A Tue Jun 30 16:34:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg68Ala' Q9HD34:p.Gln69Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Q A Tue Jun 30 17:14:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln69Ala' Q9HD34:p.Ile72Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain I A Tue Jun 30 18:40:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile72Ala' Q9HD34:p.Tyr76Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y A Tue Jun 30 18:43:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr76Ala' Q9HD34:p.Glu84Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain E A Tue Jun 30 15:48:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu84Ala' ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 4a-4h nfs1-lyrm4-1 1 5 unspecified role unspecified role bait bait purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep Q9HD34:p.Arg68Leu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R L Tue Jun 30 17:22:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg68Leu' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 physical association physical association Figure 3s