UniProt UniProt http://www.uniprot.org In vitro reassembly of the ribose ATP-binding cassette transporter reveals a distinct set of transport complexes. In vitro reassembly of the ribose ATP-binding cassette transporter reveals a distinct set of transport complexes. The Journal of biological chemistry 2015 imex curation Clifton MC., Simon MJ., Erramilli SK., Zhang H., Zaitseva J., Hermodson MA., Stauffacher CV. cstauffa@purdue.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Clifton MC., Simon MJ., Erramilli SK., Zhang H., Zaitseva J., Hermodson MA., Stauffacher CV. cstauffa@purdue.edu Accepted 2020-JAN-21 AT 09:39 GMT AT 09:39 GMT by PPORRAS The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation Mock publication and experiment for abstract interactions that are not interaction evidences. Mock publication and experiment for abstract interactions that are not interaction evidences. unspecified method In vitro reassembly of the ribose ATP-binding cassette transporter reveals a distinct set of transport complexes. In vitro reassembly of the ribose ATP-binding cassette transporter reveals a distinct set of transport complexes. The Journal of biological chemistry 2015 imex curation Clifton MC., Simon MJ., Erramilli SK., Zhang H., Zaitseva J., Hermodson MA., Stauffacher CV. cstauffa@purdue.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Clifton MC., Simon MJ., Erramilli SK., Zhang H., Zaitseva J., Hermodson MA., Stauffacher CV. cstauffa@purdue.edu Accepted 2020-JAN-21 AT 09:39 GMT AT 09:39 GMT by PPORRAS The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation In vitro reassembly of the ribose ATP-binding cassette transporter reveals a distinct set of transport complexes. In vitro reassembly of the ribose ATP-binding cassette transporter reveals a distinct set of transport complexes. The Journal of biological chemistry 2015 imex curation Clifton MC., Simon MJ., Erramilli SK., Zhang H., Zaitseva J., Hermodson MA., Stauffacher CV. cstauffa@purdue.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Clifton MC., Simon MJ., Erramilli SK., Zhang H., Zaitseva J., Hermodson MA., Stauffacher CV. cstauffa@purdue.edu Accepted 2020-JAN-21 AT 09:39 GMT AT 09:39 GMT by PPORRAS The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation In vitro reassembly of the ribose ATP-binding cassette transporter reveals a distinct set of transport complexes. In vitro reassembly of the ribose ATP-binding cassette transporter reveals a distinct set of transport complexes. The Journal of biological chemistry 2015 imex curation Clifton MC., Simon MJ., Erramilli SK., Zhang H., Zaitseva J., Hermodson MA., Stauffacher CV. cstauffa@purdue.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro atpase assay atpase assay predetermined predetermined participant predetermined Clifton MC., Simon MJ., Erramilli SK., Zhang H., Zaitseva J., Hermodson MA., Stauffacher CV. cstauffa@purdue.edu Accepted 2020-JAN-21 AT 09:39 GMT AT 09:39 GMT by PPORRAS The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation atp ATP Adenosine 5'-triphosphate Adenosine triphosphate ADENOSINE-5'-TRIPHOSPHATE Adephos Adetol Adynol Atipi ATP Atriphos Cardenosine Fosfobion Glucobasin H4atp Myotriphos Triadenyl Triphosphaden adenosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) rbsa_ecoli Ribose import ATP-binding protein RbsA rbsA b3749 JW3728 protein protein ecoli Escherichia coli (strain K12) MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKSTMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFLGREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFESKVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQFIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLRKGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISEDRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQAIGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIILVSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE A37B5F3A910A5D19 rbsc_ecoli Ribose import permease protein RbsC rbsC b3750 JW3729 protein protein ecoli Escherichia coli (strain K12) MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTINNLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVAAALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADLFGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNKIKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIVGTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ C7292EE5F0C78781 rbsb_ecoli Ribose import binding protein RbsB rbsB prlB rbsP b3751 JW3730 protein protein ecoli Escherichia coli (strain K12) MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSLKDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKMANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGTSAARERGEGFQQAVAAHKFNVLASQPADFDRIKGLNVMQNLLTAHPDVQAVFAQNDEMALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKVLKGEKVQAKYPVDLKLVVKQ DIP protein P02925 original sequence version: null E5FA305A64EF3ACE rbsc-rbsa-2 3 8 unspecified role unspecified role bait bait purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction Figs. 1A, lane 4, 2A Complex purified in the presence of Mg2+, ATP, and VO4, or in the presence of Mg2+-ADP. Addition of ribose to the rbsABC complex lead to dissociation of rbsB. rbsc-rbsa-1 3 9 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role bait bait purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) association association Fig. 1A, lane 5, 2A Complex purified in the presence of Mg2+, ATP, and VO4 rbsc-rbsb-1 3 9 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role bait bait purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Figs. 1A, lane 6, 1B, 2A rbsa-rbsc-1 3 8 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Figs. 2B Complex purified in the presence of Mg2+-ADP rbsa-rbsb-1 3 9 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) association association Fig. 2B Complex purified in the presence of Mg2+, ATP, and VO4 rbsb-rbsc-1 3 9 unspecified role unspecified role bait bait purified purified c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction Figs. 2C, 2D Complex formed in the absence of cofators or the presence of Mg-ADP. rbsc-rbsa-3 3 9 unspecified role unspecified role bait bait purified purified c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) association association Fig. 2C Complex purified in the presence of Mg2+, ATP, and VO4 rbsb-rbsc-2 1 9 unspecified role unspecified role bait bait purified purified c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction Figs. 2D Complex formed in the absence of cofators or the presence of Mg-ADP. rbsb-rbsc-3 4 9 unspecified role unspecified role neutral component neutral component purified purified P02925:p.Asn66Cys mutation mutation certain certain sequence position certain certain certain sequence position certain N C Thu Jul 11 17:45:56 BST 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn41Cys' Tue Jun 30 19:50:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn66Cys' ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 5 Complex formed in the absence of cofators or the presence of Mg-ADP. rbsa-rbsb-2 4 7 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component purified purified P02925:p.Asn66Cys mutation mutation certain certain sequence position certain certain certain sequence position certain N C Thu Jul 11 16:36:47 BST 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn41Cys' Tue Jun 30 18:14:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn66Cys' ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) physical association physical association Fig. 5 Complex formed in the absence of cofators or the presence of Mg-ADP. atp-rbsabc_ecoli-1 5 56 enzyme enzyme unspecified role unspecified role purified purified ecoli Escherichia coli (strain K12) 6 enzyme target enzyme target substrate substrate unspecified role unspecified role atpase reaction atpase reaction Fig 4 Ribose (CHEBI:33942) Vanadate (CHEBI:46442) rbsabc_ecoli RbsABC ribose ABC transporter 2xrbsA:rbsB:2xrbsC 7 enzyme enzyme rbsc binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rbsa binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role rbsb binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rbsa binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rbsc binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 enzyme enzyme rbsc binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rbsa binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role rbsc binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role rbsb binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rbsa binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rbsc binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 59 63 59 72 63 66 72 66 60 67 69 62 69 71 64 73 physical association physical association ecoli Escherichia coli (strain K12) stable complex stable complex physical interaction evidence used in manual assertion physical interaction evidence used in manual assertion IPI inferred from physical interaction Heteropentamer Required for the high affinity uptake of ribose, the molecular precursor for nucleic acid synthesis. Member of the ATP-binding cassette (ABC) transporter family, prokaryote-type (PK-type) subfamily. ATP-binding cassette (ABC) transporters are integral membrane proteins that actively transport molecules across the lipid membrane against a concentration gradient, using the energy derived from the hydrolysis of ATP. ABC transporters are composed of two transmembrane domains (TMDs) which are the integral membrane proteins and two nucleotide binding domain (NBDs) proteins associated with the TMDs on one side of the membrane. The TMDs form the transport channel and consist of several membrane-spanning alpha-helices. The NBDs bind and hydrolyse ATP. ATP binding induces conformational changes in the NBDs, forcing them into closer contact and forming the characteristic nucleotide sandwich dimer. This then causes a conformational change in the TMDs which results in the opening of the transport channel. The PK-type ABC transporters are importers that require additional periplasmic binding proteins to recruit substrates from the extracellular space and deliver them to the core transporter. Typically each component in PK-type ABC transporters is a separate protein, arising from an individual gene in an operon coding for the complete transporter. Ribose (CHEBI:33942) Accepted 2019-JUL-29 by LIVIAP