IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ NLRP7 inter-domain interactions: the NACHT-associated domain is the physical mediator for oligomeric assembly. NLRP7 inter-domain interactions: the NACHT-associated domain is the physical mediator for oligomeric assembly. Molecular human reproduction 2014 imex curation Singer H., Biswas A., Zimmer N., Messaed C., Oldenburg J., Slim R., El-Maarri O. Rare diseases - Interactions investigated in the context of Rare genetic disease osman.elmaarri@ukb.uni-bonn.de 21-Nov-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Singer H., Biswas A., Zimmer N., Messaed C., Oldenburg J., Slim R., El-Maarri O. Rare diseases - Interactions investigated in the context of Rare genetic disease osman.elmaarri@ukb.uni-bonn.de Accepted 2019-OCT-08 AT 11:24 BST AT 11:24 BST by LIVIAP Molecular human reproduction 2014 Only protein-protein interactions imex curation imex curation NLRP7 inter-domain interactions: the NACHT-associated domain is the physical mediator for oligomeric assembly. NLRP7 inter-domain interactions: the NACHT-associated domain is the physical mediator for oligomeric assembly. Molecular human reproduction 2014 imex curation Singer H., Biswas A., Zimmer N., Messaed C., Oldenburg J., Slim R., El-Maarri O. Rare diseases - Interactions investigated in the context of Rare genetic disease osman.elmaarri@ukb.uni-bonn.de 21-Nov-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Singer H., Biswas A., Zimmer N., Messaed C., Oldenburg J., Slim R., El-Maarri O. Rare diseases - Interactions investigated in the context of Rare genetic disease osman.elmaarri@ukb.uni-bonn.de Accepted 2019-OCT-08 AT 11:24 BST AT 11:24 BST by LIVIAP Molecular human reproduction 2014 Only protein-protein interactions imex curation imex curation q8wx94-3 NACHT, LRR and PYD domains-containing protein 7 NOD12 PYPAF3 NALP7 NLRP7 Nucleotide-binding oligomerization domain protein 12 PYRIN-containing APAF1-like protein 3 protein protein human Homo sapiens Human MTSPQLEWTLQTLLEQLNEDELKSFKSLLWAFPLEDVLQKTPWSEVEEADGKKLAEILVNTSSENWIRNATVNILEEMNLTELCKMAKAEMMEDGQVQEIDNPELGDAEEDSELAKPGEKEGWRNSMEKQSLVWKNTFWQGDIDNFHDDVTLRNQRFIPFLNPRTPRKLTPYTVVLHGPAGVGKTTLAKKCMLDWTDCNLSPTLRYAFYLSCKELSRMGPCSFAELISKDWPELQDDIPSILAQAQRILFVVDGLDELKVPPGALIQDICGDWEKKKPVPVLLGSLLKRKMLPRAALLVTTRPRALRDLQLLAQQPIYVRVEGFLEEDRRAYFLRHFGDEDQAMRAFELMRSNAALFQLGSAPAVCWIVCTTLKLQMEKGEDPVPTCLTRTGLFLRFLCSRFPQGAQLRGALRTLSLLAAQGLWAQMSVFHREDLERLGVQESDLRLFLDGDILRQDRVSKGCYSFIHLSFQQFLTALFYALEKEEGEDRDGHAWDIGDVQKLLSGEERLKNPDLIQVGHFLFGLANEKRAKELEATFGCRMSPDIKQELLQCKAHLHANKPLSVTDLKEVLGCLYESQEEELAKVVVAPFKEISIHLTNTSEVMHCSFSLKHCQDLQKLSLQVAKGVFLENYMDFELDIEFERCTYLTIPNWARQDLRSLRLWTDFCSLFSSNSNLKFLEVKQSFLSDSSVRILCDHVTRSTCHLQKVEIKNVTPDTAYRDFCLAFIGKKTLTHLTLAGHIEWERTMMLMLCDLLRNHKCNLQYLRLGGHCATPEQWAEFFYVLKANQSLKHLRLSANVLLDEGAMLLYKTMTRPKHFLQMLSLENCRLTEASCKDLAAVLVVSKKLTHLCLAKNPIGDTGVKFLCEGLSYPDCKLQTLVLQQCSITKLGCRYLSEALQEACSLTNLDLSINQIARGLWILCQALENPNCNLKHLRLWSCSLMPFYCQHLGSALLSNQKLETLDLGQNHLWKSGIIKLFGVLRQRTGSLKILRLKTYETNLEIKKLLEEVKEKNPKLTIDCNASGATAPPCCDFFC No experimental confirmation available. BB5642CADF5DA666 nlrp7-nlrp7-3 1 3 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain NAD domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q8WX94-3:p.Leu398Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L R Tue Jun 30 16:40:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu398Arg' Q8WX94-3:p.Lys511Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K R Tue Jun 30 15:37:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys511Arg' Q8WX94-3:p.Arg693Pro mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R P Tue Jun 30 18:56:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg693Pro' Q8WX94-3:p.Arg693Trp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R W Tue Jun 30 17:43:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg693Trp' 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain NAD domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 6 14 6 15 8 14 7 15 8 15 physical association physical association Fig 1b, Suppl Fig. 2a The full length construct of NLRP7 did not show positive interaction with self-protein nlrp7-nlrp7-1 2 3 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain NAD domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain NAD domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 19 23 24 19 23 20 24 20 physical association physical association Fig 1c, 1d nlrp7-nlrp7-2 2 3 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell Q8WX94-3:p.Leu398Arg mutation mutation certain certain sequence position certain certain certain sequence position certain L R Tue Jun 30 19:29:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu398Arg' region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q8WX94-3:p.Leu398Arg mutation mutation certain certain sequence position certain certain certain sequence position certain L R Tue Jun 30 16:58:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu398Arg' physical association physical association Fig 3a nlrp7-nlrp7-4 2 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q8WX94-3:p.Lys511Arg mutation mutation certain certain sequence position certain certain certain sequence position certain K R Tue Jun 30 15:53:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys511Arg' 3 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell Q8WX94-3:p.Lys511Arg mutation mutation certain certain sequence position certain certain certain sequence position certain K R Tue Jun 30 17:50:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys511Arg' region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 3a nlrp7-nlrp7-5 2 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q8WX94-3:p.Arg693Pro mutation mutation certain certain sequence position certain certain certain sequence position certain R P Tue Jun 30 19:19:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg693Pro' 3 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q8WX94-3:p.Arg693Pro mutation mutation certain certain sequence position certain certain certain sequence position certain R P Tue Jun 30 20:14:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg693Pro' physical association physical association Fig 3a nlrp7-nlrp7-6 2 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q8WX94-3:p.Arg693Trp mutation mutation certain certain sequence position certain certain certain sequence position certain R W Tue Jun 30 19:18:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg693Trp' 3 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q8WX94-3:p.Arg693Trp mutation mutation certain certain sequence position certain certain certain sequence position certain R W Tue Jun 30 18:49:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg693Trp' physical association physical association Fig 3a nlrp7-nlrp7-7 1 3 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain NAD domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell Q8WX94-3:p.Lys511Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K R Tue Jun 30 18:59:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys511Arg' region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain NAD domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q8WX94-3:p.Leu398Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L R Tue Jun 30 19:50:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu398Arg' Q8WX94-3:p.Arg693Pro mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R P Tue Jun 30 16:41:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg693Pro' Q8WX94-3:p.Arg693Trp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R W Tue Jun 30 16:10:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg693Trp' 60 55 55 61 60 57 61 56 57 61 physical association physical association Fig 1b, Suppl Fig. 2a The full length construct of NLRP7 did not show positive interaction with self-protein