UniProt UniProt http://www.uniprot.org WLS retrograde transport to the endoplasmic reticulum during Wnt secretion. WLS retrograde transport to the endoplasmic reticulum during Wnt secretion. Developmental cell 2014 imex curation Yu J., Chia J., Canning CA., Jones CM., Bard FA., Virshup DM., Yu J., Chia J., Canning CA., Jones CM., Bard FA., Virshup DM. Origin of WNT3A inferred from PMID:22784633. Origin of HA-PORCN inferred from PMID:26257057. Source of ERGIC2 could not be traced so interactions invovling this protein were not added. david.virshup@duke-nus.edu.sg 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Yu J., Chia J., Canning CA., Jones CM., Bard FA., Virshup DM., Yu J., Chia J., Canning CA., Jones CM., Bard FA., Virshup DM. STF cells - an HEK293 cell line that contains an integrated SuperTOPflash reporter Origin of WNT3A inferred from PMID:22784633. Origin of HA-PORCN inferred from PMID:26257057. Source of ERGIC2 could not be traced so interactions invovling this protein were not added. david.virshup@duke-nus.edu.sg Accepted 2020-MAR-24 AT 05:14 GMT AT 05:14 GMT by KALPANAP Developmental cell 2014 Only protein-protein interactions imex curation imex curation WLS retrograde transport to the endoplasmic reticulum during Wnt secretion. WLS retrograde transport to the endoplasmic reticulum during Wnt secretion. Developmental cell 2014 imex curation Yu J., Chia J., Canning CA., Jones CM., Bard FA., Virshup DM., Yu J., Chia J., Canning CA., Jones CM., Bard FA., Virshup DM. Origin of WNT3A inferred from PMID:22784633. Origin of HA-PORCN inferred from PMID:26257057. Source of ERGIC2 could not be traced so interactions invovling this protein were not added. david.virshup@duke-nus.edu.sg 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference fluorescence imaging fluorescence microscopy fluorescence imaging anti-tag immunocytochemistry anti-tag immunocytochemistry Yu J., Chia J., Canning CA., Jones CM., Bard FA., Virshup DM., Yu J., Chia J., Canning CA., Jones CM., Bard FA., Virshup DM. STF cells - an HEK293 cell line that contains an integrated SuperTOPflash reporter Origin of WNT3A inferred from PMID:22784633. Origin of HA-PORCN inferred from PMID:26257057. Source of ERGIC2 could not be traced so interactions invovling this protein were not added. david.virshup@duke-nus.edu.sg Accepted 2020-MAR-24 AT 05:14 GMT AT 05:14 GMT by KALPANAP Developmental cell 2014 Only protein-protein interactions imex curation imex curation WLS retrograde transport to the endoplasmic reticulum during Wnt secretion. WLS retrograde transport to the endoplasmic reticulum during Wnt secretion. Developmental cell 2014 imex curation Yu J., Chia J., Canning CA., Jones CM., Bard FA., Virshup DM., Yu J., Chia J., Canning CA., Jones CM., Bard FA., Virshup DM. Origin of WNT3A inferred from PMID:22784633. Origin of HA-PORCN inferred from PMID:26257057. Source of ERGIC2 could not be traced so interactions invovling this protein were not added. david.virshup@duke-nus.edu.sg 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Yu J., Chia J., Canning CA., Jones CM., Bard FA., Virshup DM., Yu J., Chia J., Canning CA., Jones CM., Bard FA., Virshup DM. Origin of WNT3A inferred from PMID:22784633. Origin of HA-PORCN inferred from PMID:26257057. Source of ERGIC2 could not be traced so interactions invovling this protein were not added. david.virshup@duke-nus.edu.sg Accepted 2020-MAR-24 AT 05:14 GMT AT 05:14 GMT by KALPANAP Developmental cell 2014 Only protein-protein interactions imex curation imex curation WLS retrograde transport to the endoplasmic reticulum during Wnt secretion. WLS retrograde transport to the endoplasmic reticulum during Wnt secretion. Developmental cell 2014 imex curation Yu J., Chia J., Canning CA., Jones CM., Bard FA., Virshup DM., Yu J., Chia J., Canning CA., Jones CM., Bard FA., Virshup DM. Origin of WNT3A inferred from PMID:22784633. Origin of HA-PORCN inferred from PMID:26257057. Source of ERGIC2 could not be traced so interactions invovling this protein were not added. david.virshup@duke-nus.edu.sg 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Yu J., Chia J., Canning CA., Jones CM., Bard FA., Virshup DM., Yu J., Chia J., Canning CA., Jones CM., Bard FA., Virshup DM. Origin of WNT3A inferred from PMID:22784633. Origin of HA-PORCN inferred from PMID:26257057. Source of ERGIC2 could not be traced so interactions invovling this protein were not added. david.virshup@duke-nus.edu.sg Accepted 2020-MAR-24 AT 05:14 GMT AT 05:14 GMT by KALPANAP Developmental cell 2014 Only protein-protein interactions imex curation imex curation wls_human Protein wntless homolog WLS C1orf139 GPR177 Integral membrane protein GPR177 Protein evenness interrupted homolog Putative NF-kappa-B-activating protein 373 UNQ85/PRO18667 protein protein human Homo sapiens Human MAGAIIENMSTKKLCIVGGILLVFQIIAFLVGGLIAPGPTTAVSYMSVKCVDARKNHHKTKWFVPWGPNHCDKIRDIEEAIPREIEANDIVFSVHIPLPHMEMSPWFQFMLFILQLDIAFKLNNQIRENAEVSMDVSLAYRDDAFAEWTEMAHERVPRKLKCTFTSPKTPEHEGRYYECDVLPFMEIGSVAHKFYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIMVWYWRRITMMSRPPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQVGPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTDIGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLIFRFKFLMLITLACAAMTVIFFIVSQVTEGHWKWGGVTVQVNSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGDLGVHSGEELQLTTTITHVDGPTEIYKLTRKEAQE 0DAFDF51BBA0C288 wnt3a_human Protein Wnt-3a WNT3A protein protein human Homo sapiens Human MAPLGYFLLLCSLKQALGSYPIWWSLAVGPQYSSLGSQPILCASIPGLVPKQLRFCRNYVEIMPSVAEGIKIGIQECQHQFRGRRWNCTTVHDSLAIFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGTAAICGCSSRHQGSPGKGWKWGGCSEDIEFGGMVSREFADARENRPDARSAMNRHNNEAGRQAIASHMHLKCKCHGLSGSCEVKTCWWSQPDFRAIGDFLKDKYDSASEMVVEKHRESRGWVETLRPRYTYFKVPTERDLVYYEASPNFCEPNPETGSFGTRDRTCNVSSHGIDGCDLLCCGRGHNARAERRREKCRCVFHWCCYVSCQECTRVYDVHTCK DIP protein P56704 original sequence version: 81 DIP protein P56704 original sequence version: 97 DIP protein P56704 original sequence version: 135 A317BD6D4A73920B porcn_mouse Protein-serine O-palmitoleoyltransferase porcupine Porcn Porc Ppn protein protein mouse Mus musculus Mouse MATFSRQEFFQQLLQGCLLPTVQQGLDQIWLLLTICFACRLLWRLGLPSYLKHASTVAGGFFSLYHFFQLHMVWVVLLSLLCYLVLFLCRHSSHRGVFLSVTILIYLLMGEMHMVDTVTWHKMRGAQMIVAMKAVSLGFDLDRGEVGAVPSPVEFMGYLYFVGTIVFGPWISFHSYLQAVQGRPLSRRWLKKVARSLALALLCLVLSTCVGPYLFPYFIPLDGDRLLRNKKRKARGTMVRWLRAYESAVSFHFSNYFVGFLSEATATLAGAGFTEEKDHLEWDLTVSRPLNVELPRSMVEVVTSWNLPMSYWLNNYVFKNALRLGTFSAVLVTYAASALLHGFSFHLAAVLLSLAFITYVEHVLRKRLAQILSACILSKRCLPDCSHRHRLGLGVRALNLLFGALAIFHLSYLGSLFDVDVDDTTEEQGYGMAYTVHKWSELSWASHWVTFGCWIFYRLIG 25A52681C251A0A6 wnt3a-wls-1 3 5 unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level 6 unspecified role unspecified role bait bait subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed c-terminal v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 1H, Supp. Fig. 1G PORCN inhibitor C59 (inhibits palmityolation) wnt3a-wls-2 1 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed Q5T9L3:p.Tyr425Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Y A Tue Jun 30 16:12:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr425Ala' p.Arg536_Glu541delinsLyslysLeuVal mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain RKEAQE KKLV 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c-terminal v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 4I PORCN inhibitor C59 (inhibits palmityolation) porcn-wls-1 4 5 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level 7 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined proximity proximity close-contact colocalization neighbourhood interaction Fig. 1I wnt3a-wls-3 2 5 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed Q5T9L3:p.Arg536Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R A Tue Jun 30 18:32:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg536Ala' Q5T9L3:p.Lys537Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 17:34:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys537Ala' Q5T9L3:p.Glu538Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A Tue Jun 30 16:50:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu538Ala' Q5T9L3:p.Gln540Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Q A Tue Jun 30 19:00:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln540Ala' Q5T9L3:p.Glu541Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A Tue Jun 30 15:43:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu541Ala' 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c-terminal v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Supp. Fig. 2A-F PORCN inhibitor C59 (inhibits palmityolation) porcn-wls-2 2 5 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level 7 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Supp Fig. 1H