IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A component of the mir-17-92 polycistronic oncomir promotes oncogene-dependent apoptosis. A component of the mir-17-92 polycistronic oncomir promotes oncogene-dependent apoptosis. eLife 2013 imex curation Olive V., Sabio E., Bennett MJ., De Jong CS., Biton A., McGann JC., Greaney SK., Sodir NM., Zhou AY., Balakrishnan A., Foth M., Luftig MA., Goga A., Speed TP., Xuan Z., Evan GI., Wan Y., Minella AC., He L. lhe@berkeley.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag luciferase tag visualisation by luciferase assay tag luciferase Olive V., Sabio E., Bennett MJ., De Jong CS., Biton A., McGann JC., Greaney SK., Sodir NM., Zhou AY., Balakrishnan A., Foth M., Luftig MA., Goga A., Speed TP., Xuan Z., Evan GI., Wan Y., Minella AC., He L. lhe@berkeley.edu Accepted 2019-DEC-03 AT 11:40 GMT AT 11:40 GMT by PPORRAS eLife 2013 Only protein-protein interactions imex curation imex curation mmu-mir -92a-3p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA mouse Mus musculus Mouse UAUUGCACUUGUCCCGGCCUG mrna_fbw7 F-box and WD-40 domain protein 7 mrna messenger rna mRNA mrna mouse Mus musculus Mouse ATTTCTTCTCTTTATATCTTTTGTGATCCCTCTACACATTGTGTCTAGACAATCAGTTTGAATTAGTGCTTGGATTTTTGCCTAGACAAAAGGAGTAACTTGGAATGAAGAAAATGGACTCAAACTTAAAGAGCTGCACACTAATCAATCCTTCGAACGCACATGGAAACCAGAGACCACCCCTACTGTCCTCTTAGTTACACAAGTGGCTGAGAATGTGAAAACCTTTGCATCTTCTGATAGTCTAGCCAAGGTCCAAGAAGTAGCAAGCTGGCTTTTGGAAATGAATCAGGAACTGCTCTCTGTGGGCAGCAAAAGACGACGAACTGGAGGCTCTCTGAGAGGGAATGCTTCCTCAAGCCAGGTTGATGAGGGACAGATGAATCGCGTGGTTGAGGAGGATCCACAGCAGCAAGCGAGACATCAAGAGGAGGAGCACACTGCGCGGAATGGTGAACTTGTGGGTGCAAACCCTAGGCCTGGAGACCAGAACGATACCCAGCAAGGACAAGTGGAGGAAAATAATAACCGCTTTATTTCAGTAGATGAGGACTCTTCGGGAAATCAGGAAGAGCAAGAGGAAGATGAAGAGCATGCTGGGGAACAGGAGGAGGAAGAGGAGGAAGAGGAAGAGGAGGAGGAGATGGACCAGGAGAGTGATGATTTTGATCCGTCTGATGACAGTAGCAGAGAAGATGAACATACGCACAATAGCAATGTCACAAACTGCAGTAGTGTCTCGGACCTGCCCGCTCACCAGCTCTCCTCTCCATTCTATACAAAGACAACAAAAATGAAAAGAAAGTTGGACCATGGTTCTGAAGTTCGTTCCTTTTCTTTGGGAAAGAAACCATGCAAAGTCTCAGATTATACCAGTACCACTGGCCTTGTACCATGTTCAGCAACACCAACAACTTTTGGGGACCTGAGAGCAGCCAATGGGCAAGGGCAGCAGCGGCGGAGGATTACATCTGTCCAACCACCCACAGGCCTTCAAGAGTGGCTGAAAATGTTTCAGAGCTGGAGCGGACCAGAGAAGTTGCTGGCTTTAGATGAGCTCATTGACAGCTGTGAACCAACACAAGTGAAGCATATGATGCAAGTGATAGAGCCCCAGTTCCAGCGAGACTTCATCTCCTTGCTTCCTAAAGAGTTGGCACTCTATGTGCTTTCATTCCTGGAACCCAAAGACCTGCTGCAAGCGGCTCAGACTTGTCGATACTGGAGAATTTTGGCTGAGGATAACCTTCTCTGGAGAGAGAAATGTAAAGAAGAGGGGATTGATGAACCGTTGCACATCAAGAGAAGAAAAATAATAAAACCAGGTTTCATACACAGCCCATGGAAGAGTGCGTATATCAGACAGCACAGAATTGATACAAACTGGAGACGAGGAGAACTCAAATCTCCTAAGGTGCTGAAAGGGCATGATGACCATGTGATCACATGCCTACAGTTTTGTGGCAACCGCATAGTTAGTGGTTCTGATGACAACACTTTAAAAGTTTGGTCAGCGGTCACGGGCAAGTGTCTGAGAACGTTAGTGGGACATACAGGTGGAGTGTGGTCATCACAGATGAGAGACAATATCATCATCAGTGGATCGACTGACCGGACTCTCAAAGTGTGGAATGCTGAAACTGGAGAGTGTATACATACTTTATATGGGCACACTTCTACTGTACGGTGTATGCATCTCCATGAAAAAAGGGTTGTAAGCGGTTCTCGAGATGCCACTCTCAGGGTTTGGGATATTGAGACCGGCCAGTGTTTACACGTCCTGATGGGTCACGTAGCAGCGGTCCGCTGCGTTCAGTATGATGGCAGGAGGGTTGTTAGTGGAGCTTATGATTTTATGGTGAAGGTGTGGGATCCAGAGACTGAGACCTGTCTACACACGTTACAGGGACACACTAATAGAGTCTATTCATTACAGTTTGATGGCATCCATGTGGTGAGTGGATCTCTTGATACATCAATCCGAGTCTGGGATGTGGAGACAGGGAATTGTATTCACACGCTAACAGGACACCAGTCATTAACGAGTGGAATGGAACTCAAAGACAATATTCTTGTCTCTGGGAATGCAGATTCTACAGTTAAGATCTGGGATATCAAAACAGGACAGTGTTTACAAACTTTGCAAGGTCCCAGCAAGCATCAGAGCGCTGTGACCTGCTTACAGTTCAACAAGAACTTCGTAATTACCAGCTCAGACGACGGAACGGTCAAACTCTGGGACTTGAAAACGGGTGAATTTATCCGAAACCTCGTCACATTGGAGAGTGGGGGGAGCGGGGGAGTTGTGTGGCGGATCAGGGCCTCAAACACAAAGCTGGTGTGTGCAGTCGGGAGTCGGAATGGAACTGAGGAAACCAAGCTCCTGGTGCTGGACTTTGATGTGGACATGAAATGAAAAGCAGACATGATGAATTTTGTCCAACTGTGTAGACAATATACTCCCTACCCTTCCCCCTGCGCAAAAAACAAAAACAAACAAACAAAAAAATGAAAAAAAAAAACAGAAAAAAAAAAAGAGAAAAAAGAAAAGGAAAAAAATCCCTTGTACTCAGTGGTGCAGGATGTTGGCTTGGGACAACAGACTGAAAAGACCTACAGACTAAGAAGGCAAGAAGAGACAAGAGACCGTAACTGACAGGAGGCGGCAGCTGTCGCATCTCGCAAAGGCCTCACTTGTGACTGAGGGGCAGCTTGGCAAGACGACTCTCTAAATCCAACCAGGTGCAATTATTCTTTGTTTTCTTCTCCAGTGGTCATTGAGCAGAGCTACATCAGCGTTGTTACCGTCACCTAGAAAGGAGTGGCAGTAATATCCAAACACGGGCTGCTTATCTTCTAATCAGAGCATCTGCAACAAACCGTCATTTTTCTGAAGTGGAAAAGCTTAAAACAATTACTGTGAATTGTTTTTGTACAGTTATCATGAAGCTTTCTTTTTTCTCTTTTTCCTGTTTCTCTTCTTTTCTTTTTTCTTTTTTTTTTTTGCCAACCATTGCCAATGTCAATCAATCACAGTATTAGCCTCTGTTAATCTATCTCTTTACTGTTGCTTCTACTCTTCAATGCATATGTTGCTCAAAGGTGGCAAGTTGTCCTGGGTTGTGTGAGTCCTGAGATGGATATAATTCTTGATGCTGGTGCTAGAAGTAGGTCTTCAAATCCGGGGTCGTTGTCCCACCCCTGTACTGTACTCCCAGTGGCCAAACTTATTTATGCTGCTAAATGAAAGAAAGAAAAAGCAAATTATTTTTTTTTTATTTTTTTTCTGCTGTGACGTTTTAGTCCCAGACTGAATTCCAAATTTGCTCTAGTTTGGTTACAGAAAAAAAAGACTTTTTTGCCACTGAAACTTGAGCCATCTGTGCCTCTAAGAGGCTGAGAATGGAAAAGTTTCAGATAATAAAGAGTGAAGTTTGCCTGCAAATAAAGAATTGAGAGTGTGTGCAAAGCTTATTTTCTTTTATCTGGGCAAAAATTAAAACACATTCCTTGGGACAGAGCCTGAGGTGCCTGTTCTGTGGAGAAACTTCTTTTTGAGGGCTGTGGTGAATGGAAGAACATACATAGCAAAACTGACAAGATATTTTAAAGATATATAAAACACAAAGGAAAAGGAGGTTGCTGGTCAGTCGTAGCATCTTACAGTATTGGGGAAAACAACTGTTACAGTTTCATTGCTCTGAGTGACTGACGTGAGAGGAATTCGCTCTGCAGTGACGCTGTCTGTCACTCGCCTACCAGCTCGACGAGCAAGAGAGCGGGAGTCAGATGGTCCGCCTCATTCACCAGGAGCCGTAACTCAAGCTGAACTGTGAAAGTGGTTAACACTGTATCCTAGGCCGTCTTTTTTTTCCTCCTCCTGTTTATTTTTTGTTTGTTTTATTTATAGTCTGATTTAAAACAATCAGATTCAAGTTGGTTAATTTTAGTTATGTAACAACCTGACGTGATGGAGGAAACAACCTGTAAAGGGATTGTGTCTATGGTTTGATTCACTTAGAAATTTTATTTTCTTATAACTTAAGTGCAATAAAATGTGTTTTTTCATGTTAAAAAAAAAAAAAAAAAAAA hsa_mir_92a 3p-mrna_fbw7-1 1 2 predetermined predetermined participant predetermined tag luciferase tag visualisation by luciferase assay tag luciferase regulator regulator modulator regulator neutral component neutral component engineered engineered purified purified 3 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered tag luciferase tag luciferase tag 3' range 3' range 3' range 3' range r.[2740_2747delinstattcttt;4043_4051delinsaaatgtgtt] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain CAGGTGCA TATTCTTT certain certain sequence position certain certain certain sequence position certain TAAGTGCAA AAATGTGTT physical association physical association Fig 5 C, D down regulate quantity by destabilization