IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Identification and profiling of CXCR3-CXCR4 chemokine receptor heteromer complexes. Identification and profiling of CXCR3-CXCR4 chemokine receptor heteromer complexes. British journal of pharmacology 2013 imex curation Watts AO., van Lipzig MM., Jaeger WC., Seeber RM., van Zwam M., Vinet J., van der Lee MM., Siderius M., Zaman GJ., Boddeke HW., Smit MJ., Pfleger KD., Leurs R., Vischer HF., Watts AO., van Lipzig MM., Jaeger WC., Seeber RM., van Zwam M., Vinet J., van der Lee MM., Siderius M., Zaman GJ., Boddeke HW., Smit MJ., Pfleger KD., Leurs R., Vischer HF. second time of curation- I maanged to delete this publication so have re-curated as that would be easier than getting developers to "find" it. So possibly I may have omitted some info?? Species origin of beta-Arrestin 2-Venus was obtained from PMID:16093556. h.f.vischer@vu.nl 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293ft Homo sapiens HEK293FT cell line 293ft HEK293FT cell line bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret tag visualisation tag visualisation Watts AO., van Lipzig MM., Jaeger WC., Seeber RM., van Zwam M., Vinet J., van der Lee MM., Siderius M., Zaman GJ., Boddeke HW., Smit MJ., Pfleger KD., Leurs R., Vischer HF., Watts AO., van Lipzig MM., Jaeger WC., Seeber RM., van Zwam M., Vinet J., van der Lee MM., Siderius M., Zaman GJ., Boddeke HW., Smit MJ., Pfleger KD., Leurs R., Vischer HF. second time of curation- I maanged to delete this publication so have re-curated as that would be easier than getting developers to "find" it. So possibly I may have omitted some info?? Species origin of beta-Arrestin 2-Venus was obtained from PMID:16093556. h.f.vischer@vu.nl Accepted 2019-MAY-23 AT 08:36 BST AT 08:36 BST by LIVIAP British journal of pharmacology 2013 Only protein-protein interactions imex curation imex curation added interaction Identification and profiling of CXCR3-CXCR4 chemokine receptor heteromer complexes. Identification and profiling of CXCR3-CXCR4 chemokine receptor heteromer complexes. British journal of pharmacology 2013 imex curation Watts AO., van Lipzig MM., Jaeger WC., Seeber RM., van Zwam M., Vinet J., van der Lee MM., Siderius M., Zaman GJ., Boddeke HW., Smit MJ., Pfleger KD., Leurs R., Vischer HF., Watts AO., van Lipzig MM., Jaeger WC., Seeber RM., van Zwam M., Vinet J., van der Lee MM., Siderius M., Zaman GJ., Boddeke HW., Smit MJ., Pfleger KD., Leurs R., Vischer HF. second time of curation- I maanged to delete this publication so have re-curated as that would be easier than getting developers to "find" it. So possibly I may have omitted some info?? Species origin of beta-Arrestin 2-Venus was obtained from PMID:16093556. h.f.vischer@vu.nl 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret tag visualisation tag visualisation Watts AO., van Lipzig MM., Jaeger WC., Seeber RM., van Zwam M., Vinet J., van der Lee MM., Siderius M., Zaman GJ., Boddeke HW., Smit MJ., Pfleger KD., Leurs R., Vischer HF., Watts AO., van Lipzig MM., Jaeger WC., Seeber RM., van Zwam M., Vinet J., van der Lee MM., Siderius M., Zaman GJ., Boddeke HW., Smit MJ., Pfleger KD., Leurs R., Vischer HF. second time of curation- I maanged to delete this publication so have re-curated as that would be easier than getting developers to "find" it. So possibly I may have omitted some info?? Species origin of beta-Arrestin 2-Venus was obtained from PMID:16093556. h.f.vischer@vu.nl Accepted 2019-MAY-23 AT 08:36 BST AT 08:36 BST by LIVIAP British journal of pharmacology 2013 Only protein-protein interactions imex curation imex curation added interaction Identification and profiling of CXCR3-CXCR4 chemokine receptor heteromer complexes. Identification and profiling of CXCR3-CXCR4 chemokine receptor heteromer complexes. British journal of pharmacology 2013 imex curation Watts AO., van Lipzig MM., Jaeger WC., Seeber RM., van Zwam M., Vinet J., van der Lee MM., Siderius M., Zaman GJ., Boddeke HW., Smit MJ., Pfleger KD., Leurs R., Vischer HF., Watts AO., van Lipzig MM., Jaeger WC., Seeber RM., van Zwam M., Vinet J., van der Lee MM., Siderius M., Zaman GJ., Boddeke HW., Smit MJ., Pfleger KD., Leurs R., Vischer HF. second time of curation- I maanged to delete this publication so have re-curated as that would be easier than getting developers to "find" it. So possibly I may have omitted some info?? Species origin of beta-Arrestin 2-Venus was obtained from PMID:16093556. h.f.vischer@vu.nl 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag visualisation tag visualisation Watts AO., van Lipzig MM., Jaeger WC., Seeber RM., van Zwam M., Vinet J., van der Lee MM., Siderius M., Zaman GJ., Boddeke HW., Smit MJ., Pfleger KD., Leurs R., Vischer HF., Watts AO., van Lipzig MM., Jaeger WC., Seeber RM., van Zwam M., Vinet J., van der Lee MM., Siderius M., Zaman GJ., Boddeke HW., Smit MJ., Pfleger KD., Leurs R., Vischer HF. second time of curation- I maanged to delete this publication so have re-curated as that would be easier than getting developers to "find" it. So possibly I may have omitted some info?? Tr-FRET: cells were labelled with Eu3+-conjugated anti-HA and XL665-conjugated anti-Flag antibodies. Species origin of beta-Arrestin 2-Venus was obtained from PMID:16093556. h.f.vischer@vu.nl Accepted 2019-MAY-23 AT 08:36 BST AT 08:36 BST by LIVIAP British journal of pharmacology 2013 Only protein-protein interactions imex curation imex curation added interaction Identification and profiling of CXCR3-CXCR4 chemokine receptor heteromer complexes. Identification and profiling of CXCR3-CXCR4 chemokine receptor heteromer complexes. British journal of pharmacology 2013 imex curation Watts AO., van Lipzig MM., Jaeger WC., Seeber RM., van Zwam M., Vinet J., van der Lee MM., Siderius M., Zaman GJ., Boddeke HW., Smit MJ., Pfleger KD., Leurs R., Vischer HF., Watts AO., van Lipzig MM., Jaeger WC., Seeber RM., van Zwam M., Vinet J., van der Lee MM., Siderius M., Zaman GJ., Boddeke HW., Smit MJ., Pfleger KD., Leurs R., Vischer HF. second time of curation- I maanged to delete this publication so have re-curated as that would be easier than getting developers to "find" it. So possibly I may have omitted some info?? Species origin of beta-Arrestin 2-Venus was obtained from PMID:16093556. h.f.vischer@vu.nl 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Watts AO., van Lipzig MM., Jaeger WC., Seeber RM., van Zwam M., Vinet J., van der Lee MM., Siderius M., Zaman GJ., Boddeke HW., Smit MJ., Pfleger KD., Leurs R., Vischer HF., Watts AO., van Lipzig MM., Jaeger WC., Seeber RM., van Zwam M., Vinet J., van der Lee MM., Siderius M., Zaman GJ., Boddeke HW., Smit MJ., Pfleger KD., Leurs R., Vischer HF. second time of curation- I maanged to delete this publication so have re-curated as that would be easier than getting developers to "find" it. So possibly I may have omitted some info?? Species origin of beta-Arrestin 2-Venus was obtained from PMID:16093556. h.f.vischer@vu.nl Accepted 2019-MAY-23 AT 08:36 BST AT 08:36 BST by LIVIAP British journal of pharmacology 2013 Only protein-protein interactions imex curation imex curation added interaction Identification and profiling of CXCR3-CXCR4 chemokine receptor heteromer complexes. Identification and profiling of CXCR3-CXCR4 chemokine receptor heteromer complexes. British journal of pharmacology 2013 imex curation Watts AO., van Lipzig MM., Jaeger WC., Seeber RM., van Zwam M., Vinet J., van der Lee MM., Siderius M., Zaman GJ., Boddeke HW., Smit MJ., Pfleger KD., Leurs R., Vischer HF., Watts AO., van Lipzig MM., Jaeger WC., Seeber RM., van Zwam M., Vinet J., van der Lee MM., Siderius M., Zaman GJ., Boddeke HW., Smit MJ., Pfleger KD., Leurs R., Vischer HF. second time of curation- I maanged to delete this publication so have re-curated as that would be easier than getting developers to "find" it. So possibly I may have omitted some info?? Species origin of beta-Arrestin 2-Venus was obtained from PMID:16093556. h.f.vischer@vu.nl 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Watts AO., van Lipzig MM., Jaeger WC., Seeber RM., van Zwam M., Vinet J., van der Lee MM., Siderius M., Zaman GJ., Boddeke HW., Smit MJ., Pfleger KD., Leurs R., Vischer HF., Watts AO., van Lipzig MM., Jaeger WC., Seeber RM., van Zwam M., Vinet J., van der Lee MM., Siderius M., Zaman GJ., Boddeke HW., Smit MJ., Pfleger KD., Leurs R., Vischer HF. second time of curation- I maanged to delete this publication so have re-curated as that would be easier than getting developers to "find" it. So possibly I may have omitted some info?? Species origin of beta-Arrestin 2-Venus was obtained from PMID:16093556. h.f.vischer@vu.nl Accepted 2019-MAY-23 AT 08:36 BST AT 08:36 BST by LIVIAP British journal of pharmacology 2013 Only protein-protein interactions imex curation imex curation added interaction arrb2_rat Beta-arrestin-2 Arrb2 Arrestin beta-2 protein protein rat Rattus norvegicus (Rat) Rat MGEKPGTRVFKKSSPNCKLTVYLGKRDFVDHLDKVDPVDGVVLVDPDYLKDRKVFVTLTCAFRYGREDLDVLGLSFRKDLFIATYQAFPPMPNPPRPPTRLQDRLLKKLGQHAHPFFFTIPQNLPCSVTLQPGPEDTGKACGVDFEIRAFCAKSIEEKSHKRNSVRLIIRKVQFAPETPGPQPSAETTRHFLMSDRRSLHLEASLDKELYYHGEPLNVNVHVTNNSAKTVKKIRVSVRQYADICLFSTAQYKCPVAQLEQDDQVSPSSTFCKVYTITPLLSDNREKRGLALDGQLKHEDTNLASSTIVKEGANKEVLGILVSYRVKVKLVVSRGGDVSVELPFVLMHPKPHDHITLPRPQSAPREIDIPVDTNLIEFDTNYATDDDIVFEDFARLRLKGMKDDDCDDQFC DIP protein P29067 original sequence version: 115 DIP protein P29067 original sequence version: 112 DIP protein P29067 original sequence version: 100 0DFA6A897C2B86BA cxcr4_human C-X-C chemokine receptor type 4 Stromal cell-derived factor 1 receptor Fusin Leukocyte-derived seven transmembrane domain receptor LCR1 FB22 NPYRL HM89 CXCR4 Lipopolysaccharide-associated protein 3 protein protein human Homo sapiens Human MEGISIYTSDNYTEEMGSGDYDSMKEPCFREENANFNKIFLPTIYSIIFLTGIVGNGLVILVMGYQKKLRSMTDKYRLHLSVADLLFVITLPFWAVDAVANWYFGNFLCKAVHVIYTVNLYSSVLILAFISLDRYLAIVHATNSQRPRKLLAEKVVYVGVWIPALLLTIPDFIFANVSEADDRYICDRFYPNDLWVVVFQFQHIMVGLILPGIVILSCYCIIISKLSHSKGHQKRKALKTTVILILAFFACWLPYYIGISIDSFILLEIIKQGCEFENTVHKWISITEALAFFHCCLNPILYAFLGAKFKTSAQHALTSVSRGSSLKILSKGKRGGHSSVSTESESSSFHSS DIP protein P61073 original sequence version: 80 8C8476A186786B83 cxcr3_human C-X-C chemokine receptor type 3 CXCR3 CKR-L2 G protein-coupled receptor 9 GPR9 Interferon-inducible protein 10 receptor protein protein human Homo sapiens Human MVLEVSDHQVLNDAEVAALLENFSSSYDYGENESDSCCTSPPCPQDFSLNFDRAFLPALYSLLFLLGLLGNGAVAAVLLSRRTALSSTDTFLLHLAVADTLLVLTLPLWAVDAAVQWVFGSGLCKVAGALFNINFYAGALLLACISFDRYLNIVHATQLYRRGPPARVTLTCLAVWGLCLLFALPDFIFLSAHHDERLNATHCQYNFPQVGRTALRVLQLVAGFLLPLLVMAYCYAHILAVLLVSRGQRRLRAMRLVVVVVVAFALCWTPYHLVVLVDILMDLGALARNCGRESRVDVAKSVTSGLGYMHCCLNPLLYAFVGVKFRERMWMLLLRLGCPNQRGLQRQPSSSRRDSSWSETSEASYSGL F08A3B44B2BBAD04 cxcr3-cxcr4-1 4 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Figure 1A cxcr3-cxcr4-2 3 7 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range proximity proximity close-contact colocalization neighbourhood interaction Figure 1B cxcr3-cxcr4-3 2 7 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed C-terminus renilla luciferase renilla luciferase protein tag renilla luciferase c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed c-terminus eyfp enhanced yellow fluorescent protein tag eyfp c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction Figure 3, Table 1-2 cxcr4-cxcr3-1 5 8 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed C-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed c-terminus venus venus fluorescent protein tag venus c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction Figure 2-2 cxcr3-cxcr4-4 2 7 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed C-terminus renilla luciferase renilla luciferase protein tag renilla luciferase c-term range C-terminal range c-term range c-term range C-terminal range c-term range 7 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed c-terminus eyfp enhanced yellow fluorescent protein tag eyfp c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction Figure 3, Table 1-1 cxcr3-cxcr4-5 2 8 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed C-terminus renilla luciferase renilla luciferase protein tag renilla luciferase c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed c-terminus eyfp enhanced yellow fluorescent protein tag eyfp c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction Figure 3, Table 1-4 cxcr4-cxcr4-1 2 8 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed C-terminus renilla luciferase renilla luciferase protein tag renilla luciferase c-term range C-terminal range c-term range c-term range C-terminal range c-term range 7 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed c-terminus eyfp enhanced yellow fluorescent protein tag eyfp c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction Figure 3, Table 1-3 cxcr3-cxcr3-1 1 8 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed C-terminus renilla luciferase renilla luciferase protein tag renilla luciferase c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed c-terminus venus venus fluorescent protein tag venus c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction Figure 4A CXCL11 arrb2-cxcr3-1 1 7 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed C-terminus renilla luciferase renilla luciferase protein tag renilla luciferase c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed c-terminus venus venus fluorescent protein tag venus c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction Figure 4B/C CXCL12 The CXCL12-induced BRET signal for beta-arrestin2-Venus recruitment to CXCR4-Rluc8 was smaller and more transient than that observed in the absence of co-expressed non-tagged CXCR3. A very weak signal was observed with CXCL11 alone, but this signal was increased when both CXCL11 and CXCL12 added simultaneously, giving a substantially stronger than that observed with CXCL12 alone. cxcr3-1 5 8 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed C-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 7 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed c-terminus venus venus fluorescent protein tag venus c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction Figure 2