UniProt UniProt http://www.uniprot.org Goodpasture antigen-binding protein/ceramide transporter binds to human serum amyloid P-component and is present in brain amyloid plaques. Goodpasture antigen-binding protein/ceramide transporter binds to human serum amyloid P-component and is present in brain amyloid plaques. The Journal of biological chemistry 2012 imex curation Mencarelli C., Bode GH., Losen M., Kulharia M., Molenaar PC., Veerhuis R., Steinbusch HW., De Baets MH., Nicolaes GA., Martinez-Martinez P. Interactions with collagen IV and laminin not added as these could not be mapped to either a single gene product or complex p.martinez@maastrichtuniversity.nl 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-plasma homo sapiens blood plasma plasma blood plasma comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel western blot western blot Immuno blot Immuno blot Mencarelli C., Bode GH., Losen M., Kulharia M., Molenaar PC., Veerhuis R., Steinbusch HW., De Baets MH., Nicolaes GA., Martinez-Martinez P. Interactions with collagen IV and laminin not added as these could not be mapped to either a single gene product or complex p.martinez@maastrichtuniversity.nl Accepted 2019-SEP-06 AT 08:33 BST AT 08:33 BST by MGT The Journal of biological chemistry 2012 Only protein-protein interactions imex curation imex curation Goodpasture antigen-binding protein/ceramide transporter binds to human serum amyloid P-component and is present in brain amyloid plaques. Goodpasture antigen-binding protein/ceramide transporter binds to human serum amyloid P-component and is present in brain amyloid plaques. The Journal of biological chemistry 2012 imex curation Mencarelli C., Bode GH., Losen M., Kulharia M., Molenaar PC., Veerhuis R., Steinbusch HW., De Baets MH., Nicolaes GA., Martinez-Martinez P. Interactions with collagen IV and laminin not added as these could not be mapped to either a single gene product or complex p.martinez@maastrichtuniversity.nl 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-brain-cortex Mus musculus brain cortex brain_cortex Brain cortex [Cerebral cortex; Cerebrum cortex; Cortex; Pallium] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mencarelli C., Bode GH., Losen M., Kulharia M., Molenaar PC., Veerhuis R., Steinbusch HW., De Baets MH., Nicolaes GA., Martinez-Martinez P. Double transgenic mice APP/PS1 expressing a chimeric mouse/human amyloid precursor protein APP KM670/671NL (Swedish) and a mutant human presenilin 1 (PS1-dE9), both directed to CNS neurons. Both mutations are associated with early-onset Alzheimer's disease. Interactions with collagen IV and laminin not added as these could not be mapped to either a single gene product or complex p.martinez@maastrichtuniversity.nl Accepted 2019-SEP-06 AT 08:33 BST AT 08:33 BST by MGT The Journal of biological chemistry 2012 Only protein-protein interactions imex curation imex curation Goodpasture antigen-binding protein/ceramide transporter binds to human serum amyloid P-component and is present in brain amyloid plaques. Goodpasture antigen-binding protein/ceramide transporter binds to human serum amyloid P-component and is present in brain amyloid plaques. The Journal of biological chemistry 2012 imex curation Mencarelli C., Bode GH., Losen M., Kulharia M., Molenaar PC., Veerhuis R., Steinbusch HW., De Baets MH., Nicolaes GA., Martinez-Martinez P. Interactions with collagen IV and laminin not added as these could not be mapped to either a single gene product or complex p.martinez@maastrichtuniversity.nl 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro mst microscale thermophoresis mst predetermined predetermined participant predetermined Mencarelli C., Bode GH., Losen M., Kulharia M., Molenaar PC., Veerhuis R., Steinbusch HW., De Baets MH., Nicolaes GA., Martinez-Martinez P. Interactions with collagen IV and laminin not added as these could not be mapped to either a single gene product or complex p.martinez@maastrichtuniversity.nl Accepted 2019-SEP-06 AT 08:33 BST AT 08:33 BST by MGT The Journal of biological chemistry 2012 Only protein-protein interactions imex curation imex curation Goodpasture antigen-binding protein/ceramide transporter binds to human serum amyloid P-component and is present in brain amyloid plaques. Goodpasture antigen-binding protein/ceramide transporter binds to human serum amyloid P-component and is present in brain amyloid plaques. The Journal of biological chemistry 2012 imex curation Mencarelli C., Bode GH., Losen M., Kulharia M., Molenaar PC., Veerhuis R., Steinbusch HW., De Baets MH., Nicolaes GA., Martinez-Martinez P. Interactions with collagen IV and laminin not added as these could not be mapped to either a single gene product or complex p.martinez@maastrichtuniversity.nl 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-plasma homo sapiens blood plasma plasma blood plasma molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Mencarelli C., Bode GH., Losen M., Kulharia M., Molenaar PC., Veerhuis R., Steinbusch HW., De Baets MH., Nicolaes GA., Martinez-Martinez P. Interactions with collagen IV and laminin not added as these could not be mapped to either a single gene product or complex p.martinez@maastrichtuniversity.nl Accepted 2019-SEP-06 AT 08:33 BST AT 08:33 BST by MGT The Journal of biological chemistry 2012 Only protein-protein interactions imex curation imex curation Goodpasture antigen-binding protein/ceramide transporter binds to human serum amyloid P-component and is present in brain amyloid plaques. Goodpasture antigen-binding protein/ceramide transporter binds to human serum amyloid P-component and is present in brain amyloid plaques. The Journal of biological chemistry 2012 imex curation Mencarelli C., Bode GH., Losen M., Kulharia M., Molenaar PC., Veerhuis R., Steinbusch HW., De Baets MH., Nicolaes GA., Martinez-Martinez P. Interactions with collagen IV and laminin not added as these could not be mapped to either a single gene product or complex p.martinez@maastrichtuniversity.nl 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-brain-cortex Homo sapiens brain cortex brain_cortex Brain cortex [Cerebral cortex; Cerebrum cortex; Cortex; Pallium] fluorescence imaging fluorescence microscopy fluorescence imaging immunohistochemistry immunohistochemistry Mencarelli C., Bode GH., Losen M., Kulharia M., Molenaar PC., Veerhuis R., Steinbusch HW., De Baets MH., Nicolaes GA., Martinez-Martinez P. Brain taken from an Alzheimer disease patient. Interactions with collagen IV and laminin not added as these could not be mapped to either a single gene product or complex p.martinez@maastrichtuniversity.nl Accepted 2019-SEP-06 AT 08:33 BST AT 08:33 BST by MGT The Journal of biological chemistry 2012 Only protein-protein interactions imex curation imex curation Goodpasture antigen-binding protein/ceramide transporter binds to human serum amyloid P-component and is present in brain amyloid plaques. Goodpasture antigen-binding protein/ceramide transporter binds to human serum amyloid P-component and is present in brain amyloid plaques. The Journal of biological chemistry 2012 imex curation Mencarelli C., Bode GH., Losen M., Kulharia M., Molenaar PC., Veerhuis R., Steinbusch HW., De Baets MH., Nicolaes GA., Martinez-Martinez P. Interactions with collagen IV and laminin not added as these could not be mapped to either a single gene product or complex p.martinez@maastrichtuniversity.nl 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Mencarelli C., Bode GH., Losen M., Kulharia M., Molenaar PC., Veerhuis R., Steinbusch HW., De Baets MH., Nicolaes GA., Martinez-Martinez P. Interactions with collagen IV and laminin not added as these could not be mapped to either a single gene product or complex p.martinez@maastrichtuniversity.nl Accepted 2019-SEP-06 AT 08:33 BST AT 08:33 BST by MGT The Journal of biological chemistry 2012 Only protein-protein interactions imex curation imex curation Goodpasture antigen-binding protein/ceramide transporter binds to human serum amyloid P-component and is present in brain amyloid plaques. Goodpasture antigen-binding protein/ceramide transporter binds to human serum amyloid P-component and is present in brain amyloid plaques. The Journal of biological chemistry 2012 imex curation Mencarelli C., Bode GH., Losen M., Kulharia M., Molenaar PC., Veerhuis R., Steinbusch HW., De Baets MH., Nicolaes GA., Martinez-Martinez P. Interactions with collagen IV and laminin not added as these could not be mapped to either a single gene product or complex p.martinez@maastrichtuniversity.nl 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot Mencarelli C., Bode GH., Losen M., Kulharia M., Molenaar PC., Veerhuis R., Steinbusch HW., De Baets MH., Nicolaes GA., Martinez-Martinez P. Interactions with collagen IV and laminin not added as these could not be mapped to either a single gene product or complex p.martinez@maastrichtuniversity.nl Accepted 2019-SEP-06 AT 08:33 BST AT 08:33 BST by MGT The Journal of biological chemistry 2012 Only protein-protein interactions imex curation imex curation Goodpasture antigen-binding protein/ceramide transporter binds to human serum amyloid P-component and is present in brain amyloid plaques. Goodpasture antigen-binding protein/ceramide transporter binds to human serum amyloid P-component and is present in brain amyloid plaques. The Journal of biological chemistry 2012 imex curation Mencarelli C., Bode GH., Losen M., Kulharia M., Molenaar PC., Veerhuis R., Steinbusch HW., De Baets MH., Nicolaes GA., Martinez-Martinez P. Interactions with collagen IV and laminin not added as these could not be mapped to either a single gene product or complex p.martinez@maastrichtuniversity.nl 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-plasma homo sapiens blood plasma plasma blood plasma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mencarelli C., Bode GH., Losen M., Kulharia M., Molenaar PC., Veerhuis R., Steinbusch HW., De Baets MH., Nicolaes GA., Martinez-Martinez P. Interactions with collagen IV and laminin not added as these could not be mapped to either a single gene product or complex p.martinez@maastrichtuniversity.nl Accepted 2019-SEP-06 AT 08:33 BST AT 08:33 BST by MGT The Journal of biological chemistry 2012 Only protein-protein interactions imex curation imex curation cert_mouse Ceramide transfer protein Cert Col4a3bp Collagen type IV alpha-3-binding protein Goodpasture antigen-binding protein START domain-containing protein 11 StAR-related lipid transfer protein 11 Stard11 Cert1 protein protein mouse Mus musculus Mouse MSDNQSWNSSGSEEDPETESGPPVERCGVLSKWTNYIHGWQDRWVVLKNNTLSYYKSEDETEYGCRGSICLSKAVITPHDFDECRFDISVNDSVWYLRAQDPEHRQQWVDAIEQHKTESGYGSESSLRRHGSMVSLVSGASGYSATSTSSFKKGHSLREKLAEMETFRDILCRQVDTLQKYFDVCADAVSKDELQRDKVVEDDEDDFPTTRSDGDFLHNTNGNKEKLFPHVTPKGINGIDFKGEAITFKATTAGILATLSHCIELMVKREESWQKRHDREVEKRRRVEEAYKNVMEELKKKPRFGGPDYEEGPNSLINEEEFFDAVEAALDRQDKIEEQSQSEKVRLHWPTSLPSGDTFSSVGTHRFVQKPYSRSSSMSSIDLVSASDDVHRFSSQVEEMVQNHMNYSLQDVGGDANWQLVVEEGEMKVYRREVEENGIVLDPLKATHAVKGVTGHEVCNYFWNVDVRNDWETTIENFHVVETLADNAIIVYQTHKRVWPASQRDVLYLSAIRKIPALTENDPETWIVCNFSVDHDSAPLNNRCVRAKINIAMICQTLVSPPEGDQEISRDNILCKITYVANVNPGGWAPASVLRAVAKREYPKFLKRFTSYVQEKTAGKPILF mint May mediate the intracellular trafficking of ceramide in a non-vesicular manner FD708AFFB25FCD31 samp_human Serum amyloid P-component APCS PTX2 9.5S alpha-1-glycoprotein protein protein human Homo sapiens Human MNKPLLWISVLTSLLEAFAHTDLSGKVFVFPRESVTDHVNLITPLEKPLQNFTLCFRAYSDLSRAYSLFSYNTQGRDNELLVYKERVGEYSLYIGRHKVTSKVIEKFPAPVHICVSWESSSGIAEFWINGTPLVKKGLRQGYFVEAQPKIVLGQEQDSYGGKFDRSQSFVGEIGDLYMWDSVLPPENILSAYQGTPLPANILDWQALNYEIRGYVIIKPLVWV DIP protein P02743 original sequence version: 159 DIP protein P02743 original sequence version: 163 6C88A515FE88B393 samp_mouse Serum amyloid P-component Apcs Ptx2 Sap protein protein mouse Mus musculus Mouse MDKLLLWMFVFTSLLSEAFCQTDLKRKVFVFPRESETDHVKLIPHLEKPLQNFTLCFRTYSDLSRSQSLFSYSVKGRDNELLIYKEKVGEYSLYIGQSKVTVRGMEEYLSPVHLCTTWESSSGIVEFWVNGKPWVKKSLQREYTVKAPPSIVLGQEQDNYGGGFQRSQSFVGEFSDLYMWDYVLTPQDILFVYRDSPVNPNILNWQALNYEINGYVVIRPRVWD 1CDBC15F45E50DCA q9y5p4-2 Ceramide transfer protein Goodpasture antigen-binding protein STARD11 START domain-containing protein 11 StAR-related lipid transfer protein 11 CERT Delta26 GPBPD26 CERT COL4A3BP CERT1 Collagen type IV alpha-3-binding protein protein protein human Homo sapiens Human MSDNQSWNSSGSEEDPETESGPPVERCGVLSKWTNYIHGWQDRWVVLKNNALSYYKSEDETEYGCRGSICLSKAVITPHDFDECRFDISVNDSVWYLRAQDPDHRQQWIDAIEQHKTESGYGSESSLRRHGSMVSLVSGASGYSATSTSSFKKGHSLREKLAEMETFRDILCRQVDTLQKYFDACADAVSKDELQRDKVVEDDEDDFPTTRSDGDFLHSTNGNKEKLFPHVTPKGINGIDFKGEAITFKATTAGILATLSHCIELMVKREDSWQKRLDKETEKKRRTEEAYKNAMTELKKKSHFGGPDYEEGPNSLINEEEFFDAVEAALDRQDKIEEQSQSEKVRLHWPTSLPSGDAFSSVGTHRFVQKVEEMVQNHMTYSLQDVGGDANWQLVVEEGEMKVYRREVEENGIVLDPLKATHAVKGVTGHEVCNYFWNVDVRNDWETTIENFHVVETLADNAIIIYQTHKRVWPASQRDVLYLSVIRKIPALTENDPETWIVCNFSVDHDSAPLNNRCVRAKINVAMICQTLVSPPEGNQEISRDNILCKITYVANVNPGGWAPASVLRAVAKREYPKFLKRFTSYVQEKTAGKPILF FC52E241DE7C5D85 cert_human Ceramide transfer protein CERT1 CERT COL4A3BP Collagen type IV alpha-3-binding protein Goodpasture antigen-binding protein STARD11 START domain-containing protein 11 StAR-related lipid transfer protein 11 protein protein human Homo sapiens Human MSDNQSWNSSGSEEDPETESGPPVERCGVLSKWTNYIHGWQDRWVVLKNNALSYYKSEDETEYGCRGSICLSKAVITPHDFDECRFDISVNDSVWYLRAQDPDHRQQWIDAIEQHKTESGYGSESSLRRHGSMVSLVSGASGYSATSTSSFKKGHSLREKLAEMETFRDILCRQVDTLQKYFDACADAVSKDELQRDKVVEDDEDDFPTTRSDGDFLHSTNGNKEKLFPHVTPKGINGIDFKGEAITFKATTAGILATLSHCIELMVKREDSWQKRLDKETEKKRRTEEAYKNAMTELKKKSHFGGPDYEEGPNSLINEEEFFDAVEAALDRQDKIEEQSQSEKVRLHWPTSLPSGDAFSSVGTHRFVQKPYSRSSSMSSIDLVSASDDVHRFSSQVEEMVQNHMTYSLQDVGGDANWQLVVEEGEMKVYRREVEENGIVLDPLKATHAVKGVTGHEVCNYFWNVDVRNDWETTIENFHVVETLADNAIIIYQTHKRVWPASQRDVLYLSVIRKIPALTENDPETWIVCNFSVDHDSAPLNNRCVRAKINVAMICQTLVSPPEGNQEISRDNILCKITYVANVNPGGWAPASVLRAVAKREYPKFLKRFTSYVQEKTAGKPILF A125162492AC5A0E p05067-pro_0000000088 Amyloid-beta precursor protein APP AD1 A4 ABPP APPI Alzheimer disease amyloid protein Amyloid precursor protein Amyloid-beta A4 protein Cerebral vascular amyloid peptide PreA4 Protease nexin-II Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human LEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDTEEYCMAVCGSAMSQSLLKTTQEPLARDPVKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHSFGADSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN 18 770 FCE3A3BD5B1BC22E q9y5p4-1 Ceramide transfer protein CERT1 CERT COL4A3BP Collagen type IV alpha-3-binding protein Goodpasture antigen-binding protein STARD11 START domain-containing protein 11 StAR-related lipid transfer protein 11 CERTL protein protein human Homo sapiens Human MSDNQSWNSSGSEEDPETESGPPVERCGVLSKWTNYIHGWQDRWVVLKNNALSYYKSEDETEYGCRGSICLSKAVITPHDFDECRFDISVNDSVWYLRAQDPDHRQQWIDAIEQHKTESGYGSESSLRRHGSMVSLVSGASGYSATSTSSFKKGHSLREKLAEMETFRDILCRQVDTLQKYFDACADAVSKDELQRDKVVEDDEDDFPTTRSDGDFLHSTNGNKEKLFPHVTPKGINGIDFKGEAITFKATTAGILATLSHCIELMVKREDSWQKRLDKETEKKRRTEEAYKNAMTELKKKSHFGGPDYEEGPNSLINEEEFFDAVEAALDRQDKIEEQSQSEKVRLHWPTSLPSGDAFSSVGTHRFVQKPYSRSSSMSSIDLVSASDDVHRFSSQVEEMVQNHMTYSLQDVGGDANWQLVVEEGEMKVYRREVEENGIVLDPLKATHAVKGVTGHEVCNYFWNVDVRNDWETTIENFHVVETLADNAIIIYQTHKRVWPASQRDVLYLSVIRKIPALTENDPETWIVCNFSVDHDSAPLNNRCVRAKINVAMICQTLVSPPEGNQEISRDNILCKITYVANVNPGGWAPASVLRAVAKREYPKFLKRFTSYVQEKTAGKPILF A125162492AC5A0E col4a3bp-apcs-2 6 10 unspecified role unspecified role prey prey purified purified human-plasma homo sapiens blood plasma plasma blood plasma 15 unspecified role unspecified role bait bait purified purified region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction Fig. 4C, 5. Ca2+ (CHEBI:29108) col4a3bp-apcs-1 6 10 unspecified role unspecified role prey prey purified purified human-plasma homo sapiens blood plasma plasma blood plasma 12 unspecified role unspecified role bait bait purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9Y5P4-2:p.[Ile413Ala;Trp562Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain W A Tue Jun 30 16:22:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile413Ala;Trp562Ala]' Q9Y5P4-2:p.Asn434_Tyr435delinsAlaAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain NY AA Tue Mar 19 10:58:10 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asn434Ala;Tyr435Ala]' Tue Jun 30 19:47:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn434_Tyr435delinsAlaAla' Q9Y5P4-2:p.Tyr435_Phe436delinsAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain YF AA Tue Mar 19 11:17:02 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr435Ala;Phe436Ala]' Tue Jun 30 20:05:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr435_Phe436delinsAlaAla' Q9Y5P4-2:p.Val472_Trp473delinsAlaAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain VW AA Tue Mar 19 11:30:09 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val472Ala;Trp473Ala]' Tue Jun 30 15:51:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val472_Trp473delinsAlaAla' Q9Y5P4-2:p.Pro474_Ala475delinsGlyGln mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain PA GQ Tue Mar 19 11:11:26 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro474Gly;Ala475Gln]' Tue Jun 30 17:34:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro474_Ala475delinsGlyGln' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 11B Ca2+ (CHEBI:29108) col4a3bp-apcs-3 7 10 unspecified role unspecified role prey prey purified purified human-plasma homo sapiens blood plasma plasma blood plasma 15 unspecified role unspecified role bait bait purified purified region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction Fig. 4A apcs-5 1 10 unspecified role unspecified role neutral component neutral component purified purified human-plasma homo sapiens blood plasma plasma blood plasma direct interaction direct interaction Fig. 4B Ca2+ (CHEBI:29108) apcs-6 1 10 unspecified role unspecified role neutral component neutral component purified purified human-plasma homo sapiens blood plasma plasma blood plasma direct interaction direct interaction Fig. 4B Ca2+ (CHEBI:29108) col4a3bp-apcs-4 3 10 unspecified role unspecified role neutral component neutral component purified purified human-plasma homo sapiens blood plasma plasma blood plasma 15 unspecified role unspecified role neutral component neutral component purified purified region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region dye labelled dye label dye labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction Fig. 6 apcs-7 4 10 unspecified role unspecified role neutral component neutral component purified purified human-plasma homo sapiens blood plasma plasma blood plasma direct interaction direct interaction Fig. 7B Ca2+ (CHEBI:29108) apcs-8 4 10 unspecified role unspecified role neutral component neutral component purified purified human-plasma homo sapiens blood plasma plasma blood plasma direct interaction direct interaction Fig. 7B Ca2+ (CHEBI:29108) col4a3bp-apcs-5 6 10 unspecified role unspecified role prey prey purified purified human-plasma homo sapiens blood plasma plasma blood plasma 15 unspecified role unspecified role bait bait purified purified region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction Fig. 7D Ca2+ (CHEBI:29108) col4a3bp-apcs-6 6 10 unspecified role unspecified role prey prey purified purified human-plasma homo sapiens blood plasma plasma blood plasma 15 unspecified role unspecified role bait bait purified purified region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction Fig. 7E Ca2+ (CHEBI:29108) col4a3bp-apcs-7 6 10 unspecified role unspecified role bait bait purified purified human-plasma homo sapiens blood plasma plasma blood plasma 15 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Fig. 4E apcs-col4a3bp-1 8 10 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 13 unspecified role unspecified role prey prey physical association physical association Fig. 8 apcs-col4a3bp-2 5 10 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level 13 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 9B app-col4a3bp-1 2 9 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 14 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed P05067-PRO_0000000088:p.Lys653_Met654delinsAsnLeu mutation mutation certain certain sequence position certain certain certain sequence position certain KM NL Tue Mar 19 12:01:24 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys653Asn;Met654Leu]' Tue Jun 30 19:19:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys653_Met654delinsAsnLeu' physical association physical association Fig. 9C apcs-app-1 2 14 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed P05067-PRO_0000000088:p.Lys653_Met654delinsAsnLeu mutation mutation certain certain sequence position certain certain certain sequence position certain KM NL Tue Mar 19 11:04:17 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys653Asn;Met654Leu]' Tue Jun 30 16:19:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys653_Met654delinsAsnLeu' 11 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Fig. 9C col4a3bp-apcs-8 7 10 unspecified role unspecified role prey prey purified purified human-plasma homo sapiens blood plasma plasma blood plasma 12 unspecified role unspecified role bait bait purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9Y5P4-2:p.[Ile413Ala;Trp562Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain W A Tue Jun 30 15:40:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile413Ala;Trp562Ala]' Q9Y5P4-2:p.Asn434_Tyr435delinsAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain NY AA Tue Mar 19 11:54:08 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asn434Ala;Tyr435Ala]' Tue Jun 30 17:10:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn434_Tyr435delinsAlaAla' Q9Y5P4-2:p.Tyr435_Phe436delinsAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain YF AA Tue Mar 19 11:21:56 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr435Ala;Phe436Ala]' Tue Jun 30 16:01:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr435_Phe436delinsAlaAla' Q9Y5P4-2:p.Val472_Trp473delinsAlaAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain VW AA Tue Mar 19 11:51:35 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val472Ala;Trp473Ala]' Tue Jun 30 18:40:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val472_Trp473delinsAlaAla' Q9Y5P4-2:p.Pro474_Ala475delinsGlyGln mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain PA GQ Tue Mar 19 11:39:04 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro474Gly;Ala475Gln]' Tue Jun 30 19:56:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro474_Ala475delinsGlyGln' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 11C Ca2+ (CHEBI:29108) col4a3bp-apcs-9 6 10 unspecified role unspecified role prey prey purified purified human-plasma homo sapiens blood plasma plasma blood plasma 12 unspecified role unspecified role bait bait purified purified region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction Figure 4D Ca2+ (CHEBI:29108) col4a3bp-apcs-10 7 10 unspecified role unspecified role prey prey purified purified human-plasma homo sapiens blood plasma plasma blood plasma 12 unspecified role unspecified role bait bait purified purified region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction Fig. 4A