IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Mapping structural determinants within third intracellular loop that direct signaling specificity of type 1 corticotropin-releasing hormone receptor. Mapping structural determinants within third intracellular loop that direct signaling specificity of type 1 corticotropin-releasing hormone receptor. The Journal of biological chemistry 2012 imex curation Punn A., Chen J., Delidaki M., Tang J., Liapakis G., Lehnert H., Levine MA., Grammatopoulos DK. d.grammatopoulos@warwick.ac.uk 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Punn A., Chen J., Delidaki M., Tang J., Liapakis G., Lehnert H., Levine MA., Grammatopoulos DK. Source of Dominant negative GalphaI (Q205L/D273N) was obtained from respective data sheet of the construct from UMR cDNA Resource Centre d.grammatopoulos@warwick.ac.uk Accepted 2019-JUN-10 AT 17:00 BST AT 17:00 BST by PPORRAS The Journal of biological chemistry 2012 Only protein-protein interactions imex curation imex curation p04899-1 Isoform 1 of Guanine nucleotide-binding protein G(i) subunit alpha-2 GNAI2 GNAI2B protein protein human Homo sapiens Human MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEEECRQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPSRADDARQLFALSCTAEEQGVLPDDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKITHSPLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF BE6D6FF720CF3DCC crfr1_human Corticotropin-releasing factor receptor 1 CRHR1 CRFR CRFR1 CRHR Corticotropin-releasing hormone receptor 1 protein protein human Homo sapiens Human MGGHPQLRLVKALLLLGLNPVSASLQDQHCESLSLASNISGLQCNASVDLIGTCWPRSPAGQLVVRPCPAFFYGVRYNTTNNGYRECLANGSWAARVNYSECQEILNEEKKSKVHYHVAVIINYLGHCISLVALLVAFVLFLRLRPGCTHWGDQADGALEVGAPWSGAPFQVRRSIRCLRNIIHWNLISAFILRNATWFVVQLTMSPEVHQSNVGWCRLVTAAYNYFHVTNFFWMFGEGCYLHTAIVLTYSTDRLRKWMFICIGWGVPFPIIVAWAIGKLYYDNEKCWFGKRPGVYTDYIYQGPMILVLLINFIFLFNIVRILMTKLRASTTSETIQYRKAVKATLVLLPLLGITYMLFFVNPGEDEVSRVVFIYFNSFLESFQGFFVSVFYCFLNSEVRSAIRKRWHRWQDKHSIRARVARAMSIPTSPTRVSFHSIKQSTAV crhr1-gnai2-1 1 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell P34998:p.Lys343Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 17:56:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys343Ala' P34998:p.Arg321Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Tue Jun 30 19:15:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg321Ala' 2 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell P04899-1:p.[Gln205Leu;Asp273Asn] mutation mutation certain certain sequence position certain certain certain sequence position certain D N certain certain sequence position certain certain certain sequence position certain Q L Tue Jun 30 18:31:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gln205Leu;Asp273Asn]' physical association physical association Figure 6d