IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Alpha-dystrobrevin-1 recruits alpha-catulin to the alpha1D-adrenergic receptor/dystrophin-associated protein complex signalosome. Alpha-dystrobrevin-1 recruits alpha-catulin to the alpha1D-adrenergic receptor/dystrophin-associated protein complex signalosome. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C., Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C. Table S2 - text states only "~30" proteins were identified - Table S2 has many more proteins than 30, with no indication which are real interactors, so have just entered those in Fig 4C;however result in fig 4D - IP, shows a very faint band for the supposed interactor PLCB2. Mouse -Dystrobrevin:The longest isoform in Uniprot is 746 aas - and was so in the earliest version of the sequence, human version is shorter. Text states : "α-DB1 and α-DB2, differ only in the length of their C-terminal domain (α-DB2 is truncated at position 570, resulting in a loss of amino acids 570→766)". Ref 20 is singularly useless. I have NOT written to authors about isoforms of DB1/2 as they have not answered previous emails, and the paper is quite old.;they probably dont know the answer either. It has not been possible to identify the isoforms of Dystrobrevin alpha used- DB1 and DB2, hence the canonical form has been used for both proteins. (based on specific antibody immunogen sequences given in Ref 44 (pmid: 9732287), a 1998 publication, DB1 could be isoform 1 or 2 and DB2 could be isoform 7 or 4 of Uniprot entry Q9D2N4.) chague@u.washington.edu,chague@uw.edu The email address chague@uw.edu was taken from a 2015 publication and thus may be the one currently in use. 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. tap tandem affinity purification TAP TAP tap sequence tag sequence tag identification sequence tag Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C., Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C. Table S2 - text states only "~30" proteins were identified - Table S2 has many more proteins than 30, with no indication which are real interactors, so have just entered those in Fig 4C;however result in fig 4D - IP, shows a very faint band for the supposed interactor PLCB2. Mouse -Dystrobrevin:The longest isoform in Uniprot is 746 aas - and was so in the earliest version of the sequence, human version is shorter. Text states : "α-DB1 and α-DB2, differ only in the length of their C-terminal domain (α-DB2 is truncated at position 570, resulting in a loss of amino acids 570→766)". Ref 20 is singularly useless. I have NOT written to authors about isoforms of DB1/2 as they have not answered previous emails, and the paper is quite old.;they probably dont know the answer either. It has not been possible to identify the isoforms of Dystrobrevin alpha used- DB1 and DB2, hence the canonical form has been used for both proteins. (based on specific antibody immunogen sequences given in Ref 44 (pmid: 9732287), a 1998 publication, DB1 could be isoform 1 or 2 and DB2 could be isoform 7 or 4 of Uniprot entry Q9D2N4.) chague@u.washington.edu,chague@uw.edu The email address chague@uw.edu was taken from a 2015 publication and thus may be the one currently in use. Accepted 2019-APR-29 AT 08:40 BST AT 08:40 BST by LIVIAP Proceedings of the National Academy of Sciences of the United States of America 2010 Only protein-protein interactions imex curation imex curation EBI-21280709:- alpha-syntrophin in Ref 10 IS Mouse, not Human:-"Mouse a-syntrophin in pBlu2SKP..." to "for subcloning into pIRESpuro-GLUE". EBI-21281178:- I removed the Bait aas 1-177 Sufficient to Bind, as I dont think it should be there either. EBI-21280734 "CASK and LIN7C are missing": these 2 proteins are not shown in fig 1 (a sort of "high quality data-set??- on what basis?) and I cannot find these 2 proteins on Table S1 either- given that Table S1 has a lot of contaminants (HSPs/Keratins/tubulins) presumably I was acting on the fact we should err on the side of NOT adding interactors rather than adding if unsure. I think the statement that they found these proteins refers to Ref. 18, which does indeed mention these proteins - so the text is deliberately ambiguous...; altered as suggested.; yes, I agree - 1-277 is NOT "sufficient to Bind" - I have altered so the shortest construct is now Suff. to Bind + deletion region added as Required to Bind. Hope this is OK. The image is very poor quality.; Figure S5: I have added a comment - there are at least 5 Syntrophins and no idea which one(s) detected here - I have added a comment to the existing interaction. Alpha-dystrobrevin-1 recruits alpha-catulin to the alpha1D-adrenergic receptor/dystrophin-associated protein complex signalosome. Alpha-dystrobrevin-1 recruits alpha-catulin to the alpha1D-adrenergic receptor/dystrophin-associated protein complex signalosome. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C., Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C. Table S2 - text states only "~30" proteins were identified - Table S2 has many more proteins than 30, with no indication which are real interactors, so have just entered those in Fig 4C;however result in fig 4D - IP, shows a very faint band for the supposed interactor PLCB2. Mouse -Dystrobrevin:The longest isoform in Uniprot is 746 aas - and was so in the earliest version of the sequence, human version is shorter. Text states : "α-DB1 and α-DB2, differ only in the length of their C-terminal domain (α-DB2 is truncated at position 570, resulting in a loss of amino acids 570→766)". Ref 20 is singularly useless. I have NOT written to authors about isoforms of DB1/2 as they have not answered previous emails, and the paper is quite old.;they probably dont know the answer either. It has not been possible to identify the isoforms of Dystrobrevin alpha used- DB1 and DB2, hence the canonical form has been used for both proteins. (based on specific antibody immunogen sequences given in Ref 44 (pmid: 9732287), a 1998 publication, DB1 could be isoform 1 or 2 and DB2 could be isoform 7 or 4 of Uniprot entry Q9D2N4.) chague@u.washington.edu,chague@uw.edu The email address chague@uw.edu was taken from a 2015 publication and thus may be the one currently in use. 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C., Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C. Table S2 - text states only "~30" proteins were identified - Table S2 has many more proteins than 30, with no indication which are real interactors, so have just entered those in Fig 4C;however result in fig 4D - IP, shows a very faint band for the supposed interactor PLCB2. Mouse -Dystrobrevin:The longest isoform in Uniprot is 746 aas - and was so in the earliest version of the sequence, human version is shorter. Text states : "α-DB1 and α-DB2, differ only in the length of their C-terminal domain (α-DB2 is truncated at position 570, resulting in a loss of amino acids 570→766)". Ref 20 is singularly useless. I have NOT written to authors about isoforms of DB1/2 as they have not answered previous emails, and the paper is quite old.;they probably dont know the answer either. It has not been possible to identify the isoforms of Dystrobrevin alpha used- DB1 and DB2, hence the canonical form has been used for both proteins. (based on specific antibody immunogen sequences given in Ref 44 (pmid: 9732287), a 1998 publication, DB1 could be isoform 1 or 2 and DB2 could be isoform 7 or 4 of Uniprot entry Q9D2N4.) chague@u.washington.edu,chague@uw.edu The email address chague@uw.edu was taken from a 2015 publication and thus may be the one currently in use. Accepted 2019-APR-29 AT 08:40 BST AT 08:40 BST by LIVIAP Proceedings of the National Academy of Sciences of the United States of America 2010 Only protein-protein interactions imex curation imex curation EBI-21280709:- alpha-syntrophin in Ref 10 IS Mouse, not Human:-"Mouse a-syntrophin in pBlu2SKP..." to "for subcloning into pIRESpuro-GLUE". EBI-21281178:- I removed the Bait aas 1-177 Sufficient to Bind, as I dont think it should be there either. EBI-21280734 "CASK and LIN7C are missing": these 2 proteins are not shown in fig 1 (a sort of "high quality data-set??- on what basis?) and I cannot find these 2 proteins on Table S1 either- given that Table S1 has a lot of contaminants (HSPs/Keratins/tubulins) presumably I was acting on the fact we should err on the side of NOT adding interactors rather than adding if unsure. I think the statement that they found these proteins refers to Ref. 18, which does indeed mention these proteins - so the text is deliberately ambiguous...; altered as suggested.; yes, I agree - 1-277 is NOT "sufficient to Bind" - I have altered so the shortest construct is now Suff. to Bind + deletion region added as Required to Bind. Hope this is OK. The image is very poor quality.; Figure S5: I have added a comment - there are at least 5 Syntrophins and no idea which one(s) detected here - I have added a comment to the existing interaction. Alpha-dystrobrevin-1 recruits alpha-catulin to the alpha1D-adrenergic receptor/dystrophin-associated protein complex signalosome. Alpha-dystrobrevin-1 recruits alpha-catulin to the alpha1D-adrenergic receptor/dystrophin-associated protein complex signalosome. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C., Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C. Table S2 - text states only "~30" proteins were identified - Table S2 has many more proteins than 30, with no indication which are real interactors, so have just entered those in Fig 4C;however result in fig 4D - IP, shows a very faint band for the supposed interactor PLCB2. Mouse -Dystrobrevin:The longest isoform in Uniprot is 746 aas - and was so in the earliest version of the sequence, human version is shorter. Text states : "α-DB1 and α-DB2, differ only in the length of their C-terminal domain (α-DB2 is truncated at position 570, resulting in a loss of amino acids 570→766)". Ref 20 is singularly useless. I have NOT written to authors about isoforms of DB1/2 as they have not answered previous emails, and the paper is quite old.;they probably dont know the answer either. It has not been possible to identify the isoforms of Dystrobrevin alpha used- DB1 and DB2, hence the canonical form has been used for both proteins. (based on specific antibody immunogen sequences given in Ref 44 (pmid: 9732287), a 1998 publication, DB1 could be isoform 1 or 2 and DB2 could be isoform 7 or 4 of Uniprot entry Q9D2N4.) chague@u.washington.edu,chague@uw.edu The email address chague@uw.edu was taken from a 2015 publication and thus may be the one currently in use. 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C., Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C. Table S2 - text states only "~30" proteins were identified - Table S2 has many more proteins than 30, with no indication which are real interactors, so have just entered those in Fig 4C;however result in fig 4D - IP, shows a very faint band for the supposed interactor PLCB2. Mouse -Dystrobrevin:The longest isoform in Uniprot is 746 aas - and was so in the earliest version of the sequence, human version is shorter. Text states : "α-DB1 and α-DB2, differ only in the length of their C-terminal domain (α-DB2 is truncated at position 570, resulting in a loss of amino acids 570→766)". Ref 20 is singularly useless. I have NOT written to authors about isoforms of DB1/2 as they have not answered previous emails, and the paper is quite old.;they probably dont know the answer either. It has not been possible to identify the isoforms of Dystrobrevin alpha used- DB1 and DB2, hence the canonical form has been used for both proteins. (based on specific antibody immunogen sequences given in Ref 44 (pmid: 9732287), a 1998 publication, DB1 could be isoform 1 or 2 and DB2 could be isoform 7 or 4 of Uniprot entry Q9D2N4.) chague@u.washington.edu,chague@uw.edu The email address chague@uw.edu was taken from a 2015 publication and thus may be the one currently in use. Accepted 2019-APR-29 AT 08:40 BST AT 08:40 BST by LIVIAP Proceedings of the National Academy of Sciences of the United States of America 2010 Only protein-protein interactions imex curation imex curation EBI-21280709:- alpha-syntrophin in Ref 10 IS Mouse, not Human:-"Mouse a-syntrophin in pBlu2SKP..." to "for subcloning into pIRESpuro-GLUE". EBI-21281178:- I removed the Bait aas 1-177 Sufficient to Bind, as I dont think it should be there either. EBI-21280734 "CASK and LIN7C are missing": these 2 proteins are not shown in fig 1 (a sort of "high quality data-set??- on what basis?) and I cannot find these 2 proteins on Table S1 either- given that Table S1 has a lot of contaminants (HSPs/Keratins/tubulins) presumably I was acting on the fact we should err on the side of NOT adding interactors rather than adding if unsure. I think the statement that they found these proteins refers to Ref. 18, which does indeed mention these proteins - so the text is deliberately ambiguous...; altered as suggested.; yes, I agree - 1-277 is NOT "sufficient to Bind" - I have altered so the shortest construct is now Suff. to Bind + deletion region added as Required to Bind. Hope this is OK. The image is very poor quality.; Figure S5: I have added a comment - there are at least 5 Syntrophins and no idea which one(s) detected here - I have added a comment to the existing interaction. Alpha-dystrobrevin-1 recruits alpha-catulin to the alpha1D-adrenergic receptor/dystrophin-associated protein complex signalosome. Alpha-dystrobrevin-1 recruits alpha-catulin to the alpha1D-adrenergic receptor/dystrophin-associated protein complex signalosome. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C., Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C. Table S2 - text states only "~30" proteins were identified - Table S2 has many more proteins than 30, with no indication which are real interactors, so have just entered those in Fig 4C;however result in fig 4D - IP, shows a very faint band for the supposed interactor PLCB2. Mouse -Dystrobrevin:The longest isoform in Uniprot is 746 aas - and was so in the earliest version of the sequence, human version is shorter. Text states : "α-DB1 and α-DB2, differ only in the length of their C-terminal domain (α-DB2 is truncated at position 570, resulting in a loss of amino acids 570→766)". Ref 20 is singularly useless. I have NOT written to authors about isoforms of DB1/2 as they have not answered previous emails, and the paper is quite old.;they probably dont know the answer either. It has not been possible to identify the isoforms of Dystrobrevin alpha used- DB1 and DB2, hence the canonical form has been used for both proteins. (based on specific antibody immunogen sequences given in Ref 44 (pmid: 9732287), a 1998 publication, DB1 could be isoform 1 or 2 and DB2 could be isoform 7 or 4 of Uniprot entry Q9D2N4.) chague@u.washington.edu,chague@uw.edu The email address chague@uw.edu was taken from a 2015 publication and thus may be the one currently in use. 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. tap tandem affinity purification TAP TAP tap western blot western blot Immuno blot Immuno blot Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C., Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C. Table S2 - text states only "~30" proteins were identified - Table S2 has many more proteins than 30, with no indication which are real interactors, so have just entered those in Fig 4C;however result in fig 4D - IP, shows a very faint band for the supposed interactor PLCB2. Mouse -Dystrobrevin:The longest isoform in Uniprot is 746 aas - and was so in the earliest version of the sequence, human version is shorter. Text states : "α-DB1 and α-DB2, differ only in the length of their C-terminal domain (α-DB2 is truncated at position 570, resulting in a loss of amino acids 570→766)". Ref 20 is singularly useless. I have NOT written to authors about isoforms of DB1/2 as they have not answered previous emails, and the paper is quite old.;they probably dont know the answer either. It has not been possible to identify the isoforms of Dystrobrevin alpha used- DB1 and DB2, hence the canonical form has been used for both proteins. (based on specific antibody immunogen sequences given in Ref 44 (pmid: 9732287), a 1998 publication, DB1 could be isoform 1 or 2 and DB2 could be isoform 7 or 4 of Uniprot entry Q9D2N4.) chague@u.washington.edu,chague@uw.edu The email address chague@uw.edu was taken from a 2015 publication and thus may be the one currently in use. Accepted 2019-APR-29 AT 08:40 BST AT 08:40 BST by LIVIAP Proceedings of the National Academy of Sciences of the United States of America 2010 Only protein-protein interactions imex curation imex curation EBI-21280709:- alpha-syntrophin in Ref 10 IS Mouse, not Human:-"Mouse a-syntrophin in pBlu2SKP..." to "for subcloning into pIRESpuro-GLUE". EBI-21281178:- I removed the Bait aas 1-177 Sufficient to Bind, as I dont think it should be there either. EBI-21280734 "CASK and LIN7C are missing": these 2 proteins are not shown in fig 1 (a sort of "high quality data-set??- on what basis?) and I cannot find these 2 proteins on Table S1 either- given that Table S1 has a lot of contaminants (HSPs/Keratins/tubulins) presumably I was acting on the fact we should err on the side of NOT adding interactors rather than adding if unsure. I think the statement that they found these proteins refers to Ref. 18, which does indeed mention these proteins - so the text is deliberately ambiguous...; altered as suggested.; yes, I agree - 1-277 is NOT "sufficient to Bind" - I have altered so the shortest construct is now Suff. to Bind + deletion region added as Required to Bind. Hope this is OK. The image is very poor quality.; Figure S5: I have added a comment - there are at least 5 Syntrophins and no idea which one(s) detected here - I have added a comment to the existing interaction. Alpha-dystrobrevin-1 recruits alpha-catulin to the alpha1D-adrenergic receptor/dystrophin-associated protein complex signalosome. Alpha-dystrobrevin-1 recruits alpha-catulin to the alpha1D-adrenergic receptor/dystrophin-associated protein complex signalosome. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C., Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C. Table S2 - text states only "~30" proteins were identified - Table S2 has many more proteins than 30, with no indication which are real interactors, so have just entered those in Fig 4C;however result in fig 4D - IP, shows a very faint band for the supposed interactor PLCB2. Mouse -Dystrobrevin:The longest isoform in Uniprot is 746 aas - and was so in the earliest version of the sequence, human version is shorter. Text states : "α-DB1 and α-DB2, differ only in the length of their C-terminal domain (α-DB2 is truncated at position 570, resulting in a loss of amino acids 570→766)". Ref 20 is singularly useless. I have NOT written to authors about isoforms of DB1/2 as they have not answered previous emails, and the paper is quite old.;they probably dont know the answer either. It has not been possible to identify the isoforms of Dystrobrevin alpha used- DB1 and DB2, hence the canonical form has been used for both proteins. (based on specific antibody immunogen sequences given in Ref 44 (pmid: 9732287), a 1998 publication, DB1 could be isoform 1 or 2 and DB2 could be isoform 7 or 4 of Uniprot entry Q9D2N4.) chague@u.washington.edu,chague@uw.edu The email address chague@uw.edu was taken from a 2015 publication and thus may be the one currently in use. 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. pull down pull down western blot western blot Immuno blot Immuno blot Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C., Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C. Table S2 - text states only "~30" proteins were identified - Table S2 has many more proteins than 30, with no indication which are real interactors, so have just entered those in Fig 4C;however result in fig 4D - IP, shows a very faint band for the supposed interactor PLCB2. Mouse -Dystrobrevin:The longest isoform in Uniprot is 746 aas - and was so in the earliest version of the sequence, human version is shorter. Text states : "α-DB1 and α-DB2, differ only in the length of their C-terminal domain (α-DB2 is truncated at position 570, resulting in a loss of amino acids 570→766)". Ref 20 is singularly useless. I have NOT written to authors about isoforms of DB1/2 as they have not answered previous emails, and the paper is quite old.;they probably dont know the answer either. It has not been possible to identify the isoforms of Dystrobrevin alpha used- DB1 and DB2, hence the canonical form has been used for both proteins. (based on specific antibody immunogen sequences given in Ref 44 (pmid: 9732287), a 1998 publication, DB1 could be isoform 1 or 2 and DB2 could be isoform 7 or 4 of Uniprot entry Q9D2N4.) chague@u.washington.edu,chague@uw.edu The email address chague@uw.edu was taken from a 2015 publication and thus may be the one currently in use. Accepted 2019-APR-29 AT 08:40 BST AT 08:40 BST by LIVIAP Proceedings of the National Academy of Sciences of the United States of America 2010 Only protein-protein interactions imex curation imex curation EBI-21280709:- alpha-syntrophin in Ref 10 IS Mouse, not Human:-"Mouse a-syntrophin in pBlu2SKP..." to "for subcloning into pIRESpuro-GLUE". EBI-21281178:- I removed the Bait aas 1-177 Sufficient to Bind, as I dont think it should be there either. EBI-21280734 "CASK and LIN7C are missing": these 2 proteins are not shown in fig 1 (a sort of "high quality data-set??- on what basis?) and I cannot find these 2 proteins on Table S1 either- given that Table S1 has a lot of contaminants (HSPs/Keratins/tubulins) presumably I was acting on the fact we should err on the side of NOT adding interactors rather than adding if unsure. I think the statement that they found these proteins refers to Ref. 18, which does indeed mention these proteins - so the text is deliberately ambiguous...; altered as suggested.; yes, I agree - 1-277 is NOT "sufficient to Bind" - I have altered so the shortest construct is now Suff. to Bind + deletion region added as Required to Bind. Hope this is OK. The image is very poor quality.; Figure S5: I have added a comment - there are at least 5 Syntrophins and no idea which one(s) detected here - I have added a comment to the existing interaction. Alpha-dystrobrevin-1 recruits alpha-catulin to the alpha1D-adrenergic receptor/dystrophin-associated protein complex signalosome. Alpha-dystrobrevin-1 recruits alpha-catulin to the alpha1D-adrenergic receptor/dystrophin-associated protein complex signalosome. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C., Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C. Table S2 - text states only "~30" proteins were identified - Table S2 has many more proteins than 30, with no indication which are real interactors, so have just entered those in Fig 4C;however result in fig 4D - IP, shows a very faint band for the supposed interactor PLCB2. Mouse -Dystrobrevin:The longest isoform in Uniprot is 746 aas - and was so in the earliest version of the sequence, human version is shorter. Text states : "α-DB1 and α-DB2, differ only in the length of their C-terminal domain (α-DB2 is truncated at position 570, resulting in a loss of amino acids 570→766)". Ref 20 is singularly useless. I have NOT written to authors about isoforms of DB1/2 as they have not answered previous emails, and the paper is quite old.;they probably dont know the answer either. It has not been possible to identify the isoforms of Dystrobrevin alpha used- DB1 and DB2, hence the canonical form has been used for both proteins. (based on specific antibody immunogen sequences given in Ref 44 (pmid: 9732287), a 1998 publication, DB1 could be isoform 1 or 2 and DB2 could be isoform 7 or 4 of Uniprot entry Q9D2N4.) chague@u.washington.edu,chague@uw.edu The email address chague@uw.edu was taken from a 2015 publication and thus may be the one currently in use. 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting anti tag western anti tag western blot anti tag western Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C., Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C. Table S2 - text states only "~30" proteins were identified - Table S2 has many more proteins than 30, with no indication which are real interactors, so have just entered those in Fig 4C;however result in fig 4D - IP, shows a very faint band for the supposed interactor PLCB2. Mouse -Dystrobrevin:The longest isoform in Uniprot is 746 aas - and was so in the earliest version of the sequence, human version is shorter. Text states : "α-DB1 and α-DB2, differ only in the length of their C-terminal domain (α-DB2 is truncated at position 570, resulting in a loss of amino acids 570→766)". Ref 20 is singularly useless. I have NOT written to authors about isoforms of DB1/2 as they have not answered previous emails, and the paper is quite old.;they probably dont know the answer either. It has not been possible to identify the isoforms of Dystrobrevin alpha used- DB1 and DB2, hence the canonical form has been used for both proteins. (based on specific antibody immunogen sequences given in Ref 44 (pmid: 9732287), a 1998 publication, DB1 could be isoform 1 or 2 and DB2 could be isoform 7 or 4 of Uniprot entry Q9D2N4.) chague@u.washington.edu,chague@uw.edu The email address chague@uw.edu was taken from a 2015 publication and thus may be the one currently in use. Accepted 2019-APR-29 AT 08:40 BST AT 08:40 BST by LIVIAP Proceedings of the National Academy of Sciences of the United States of America 2010 Only protein-protein interactions imex curation imex curation EBI-21280709:- alpha-syntrophin in Ref 10 IS Mouse, not Human:-"Mouse a-syntrophin in pBlu2SKP..." to "for subcloning into pIRESpuro-GLUE". EBI-21281178:- I removed the Bait aas 1-177 Sufficient to Bind, as I dont think it should be there either. EBI-21280734 "CASK and LIN7C are missing": these 2 proteins are not shown in fig 1 (a sort of "high quality data-set??- on what basis?) and I cannot find these 2 proteins on Table S1 either- given that Table S1 has a lot of contaminants (HSPs/Keratins/tubulins) presumably I was acting on the fact we should err on the side of NOT adding interactors rather than adding if unsure. I think the statement that they found these proteins refers to Ref. 18, which does indeed mention these proteins - so the text is deliberately ambiguous...; altered as suggested.; yes, I agree - 1-277 is NOT "sufficient to Bind" - I have altered so the shortest construct is now Suff. to Bind + deletion region added as Required to Bind. Hope this is OK. The image is very poor quality.; Figure S5: I have added a comment - there are at least 5 Syntrophins and no idea which one(s) detected here - I have added a comment to the existing interaction. Alpha-dystrobrevin-1 recruits alpha-catulin to the alpha1D-adrenergic receptor/dystrophin-associated protein complex signalosome. Alpha-dystrobrevin-1 recruits alpha-catulin to the alpha1D-adrenergic receptor/dystrophin-associated protein complex signalosome. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C., Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C. Table S2 - text states only "~30" proteins were identified - Table S2 has many more proteins than 30, with no indication which are real interactors, so have just entered those in Fig 4C;however result in fig 4D - IP, shows a very faint band for the supposed interactor PLCB2. Mouse -Dystrobrevin:The longest isoform in Uniprot is 746 aas - and was so in the earliest version of the sequence, human version is shorter. Text states : "α-DB1 and α-DB2, differ only in the length of their C-terminal domain (α-DB2 is truncated at position 570, resulting in a loss of amino acids 570→766)". Ref 20 is singularly useless. I have NOT written to authors about isoforms of DB1/2 as they have not answered previous emails, and the paper is quite old.;they probably dont know the answer either. It has not been possible to identify the isoforms of Dystrobrevin alpha used- DB1 and DB2, hence the canonical form has been used for both proteins. (based on specific antibody immunogen sequences given in Ref 44 (pmid: 9732287), a 1998 publication, DB1 could be isoform 1 or 2 and DB2 could be isoform 7 or 4 of Uniprot entry Q9D2N4.) chague@u.washington.edu,chague@uw.edu The email address chague@uw.edu was taken from a 2015 publication and thus may be the one currently in use. 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-skeletal-muscle Mus musculus skeletal muscle. skeletal_muscle Skeletal muscle confocal microscopy confocal microscopy immunohistochemistry immunohistochemistry Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C., Lyssand JS., Whiting JL., Lee KS., Kastl R., Wacker JL., Bruchas MR., Miyatake M., Langeberg LK., Chavkin C., Scott JD., Gardner RG., Adams ME., Hague C. Table S2 - text states only "~30" proteins were identified - Table S2 has many more proteins than 30, with no indication which are real interactors, so have just entered those in Fig 4C;however result in fig 4D - IP, shows a very faint band for the supposed interactor PLCB2. Mouse -Dystrobrevin:The longest isoform in Uniprot is 746 aas - and was so in the earliest version of the sequence, human version is shorter. Text states : "α-DB1 and α-DB2, differ only in the length of their C-terminal domain (α-DB2 is truncated at position 570, resulting in a loss of amino acids 570→766)". Ref 20 is singularly useless. I have NOT written to authors about isoforms of DB1/2 as they have not answered previous emails, and the paper is quite old.;they probably dont know the answer either. murine quadriceps It has not been possible to identify the isoforms of Dystrobrevin alpha used- DB1 and DB2, hence the canonical form has been used for both proteins. (based on specific antibody immunogen sequences given in Ref 44 (pmid: 9732287), a 1998 publication, DB1 could be isoform 1 or 2 and DB2 could be isoform 7 or 4 of Uniprot entry Q9D2N4.) chague@u.washington.edu,chague@uw.edu The email address chague@uw.edu was taken from a 2015 publication and thus may be the one currently in use. Accepted 2019-APR-29 AT 08:40 BST AT 08:40 BST by LIVIAP Proceedings of the National Academy of Sciences of the United States of America 2010 Only protein-protein interactions imex curation imex curation EBI-21280709:- alpha-syntrophin in Ref 10 IS Mouse, not Human:-"Mouse a-syntrophin in pBlu2SKP..." to "for subcloning into pIRESpuro-GLUE". EBI-21281178:- I removed the Bait aas 1-177 Sufficient to Bind, as I dont think it should be there either. EBI-21280734 "CASK and LIN7C are missing": these 2 proteins are not shown in fig 1 (a sort of "high quality data-set??- on what basis?) and I cannot find these 2 proteins on Table S1 either- given that Table S1 has a lot of contaminants (HSPs/Keratins/tubulins) presumably I was acting on the fact we should err on the side of NOT adding interactors rather than adding if unsure. I think the statement that they found these proteins refers to Ref. 18, which does indeed mention these proteins - so the text is deliberately ambiguous...; altered as suggested.; yes, I agree - 1-277 is NOT "sufficient to Bind" - I have altered so the shortest construct is now Suff. to Bind + deletion region added as Required to Bind. Hope this is OK. The image is very poor quality.; Figure S5: I have added a comment - there are at least 5 Syntrophins and no idea which one(s) detected here - I have added a comment to the existing interaction. sntb2_human Beta-2-syntrophin SNT2B2 SNTB2 D16S2531E SNTL 59 kDa dystrophin-associated protein A1 basic component 2 Syntrophin-3 Syntrophin-like protein protein human Homo sapiens Human MRVAAATAAAGAGPAMAVWTRATKAGLVELLLRERWVRVVAELSGESLSLTGDAAAAELEPALGPAAAAFNGLPNGGGAGDSLPGSPSRGLGPPSPPAPPRGPAGEAGASPPVRRVRVVKQEAGGLGISIKGGRENRMPILISKIFPGLAADQSRALRLGDAILSVNGTDLRQATHDQAVQALKRAGKEVLLEVKFIREVTPYIKKPSLVSDLPWEGAAPQSPSFSGSEDSGSPKHQNSTKDRKIIPLKMCFAARNLSMPDLENRLIELHSPDSRNTLILRCKDTATAHSWFVAIHTNIMALLPQVLAELNAMLGATSTAGGSKEVKHIAWLAEQAKLDGGRQQWRPVLMAVTEKDLLLYDCMPWTRDAWASPCHSYPLVATRLVHSGSGCRSPSLGSDLTFATRTGSRQGIEMHLFRVETHRDLSSWTRILVQGCHAAAELIKEVSLGCMLNGQEVRLTIHYENGFTISRENGGSSSILYRYPFERLKMSADDGIRNLYLDFGGPEGELTMDLHSCPKPIVFVLHTFLSAKVTRMGLLV 53A9F3340DC024ED dmd_human Dystrophin DMD protein protein human Homo sapiens Human MLWWEEVEDCYEREDVQKKTFTKWVNAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALRVLQNNNVDLVNIGSTDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVCQQSATQRLEHAFNIARYQLGIEKLLDPEDVDTTYPDKKSILMYITSLFQVLPQQVSIEAIQEVEMLPRPPKVTKEEHFQLHHQMHYSQQITVSLAQGYERTSSPKPRFKSYAYTQAAYVTTSDPTRSPFPSQHLEAPEDKSFGSSLMESEVNLDRYQTALEEVLSWLLSAEDTLQAQGEISNDVEVVKDQFHTHEGYMMDLTAHQGRVGNILQLGSKLIGTGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHRVLMDLQNQKLKELNDWLTKTEERTRKMEEEPLGPDLEDLKRQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDILLKWQRLTEEQCLFSAWLSEKEDAVNKIHTTGFKDQNEMLSSLQKLAVLKADLEKKKQSMGKLYSLKQDLLSTLKNKSVTQKTEAWLDNFARCWDNLVQKLEKSTAQISQAVTTTQPSLTQTTVMETVTTVTTREQILVKHAQEELPPPPPQKKRQITVDSEIRKRLDVDITELHSWITRSEAVLQSPEFAIFRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIIAFYNQLQQLEQMTTTAENWLKIQPTTPSEPTAIKSQLKICKDEVNRLSDLQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFKQVFSDVQAREKELQTIFDTLPPMRYQETMSAIRTWVQQSETKLSIPQLSVTDYEIMEQRLGELQALQSSLQEQQSGLYYLSTTVKEMSKKAPSEISRKYQSEFEEIEGRWKKLSSQLVEHCQKLEEQMNKLRKIQNHIQTLKKWMAEVDVFLKEEWPALGDSEILKKQLKQCRLLVSDIQTIQPSLNSVNEGGQKIKNEAEPEFASRLETELKELNTQWDHMCQQVYARKEALKGGLEKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQKAVEEMKRAKEEAQQKEAKVKLLTESVNSVIAQAPPVAQEALKKELETLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLNEVEFKLKTTENIPGGAEEISEVLDSLENLMRHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQETEKSLHLIQESLTFIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKEAAQRVLSQIDVAQKKLQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNVLTEWLAATDMELTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKVHLKSITEVGEALKTVLGKKETLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMETFDQNVDHITKWIIQADTLLDESEKKKPQQKEDVLKRLKAELNDIRPKVDSTRDQAANLMANRGDHCRKLVEPQISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMNEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVRRQAEGLSEDGAAMAVEPTQIQLSKRWREIESKFAQFRRLNFAQIHTVREETMMVMTEDMPLEISYVPSTYLTEITHVSQALLEVEQLLNAPDLCAKDFEDLFKQEESLKNIKDSLQQSSGRIDIIHSKKTAALQSATPVERVKLQEALSQLDFQWEKVNKMYKDRQGRFDRSVEKWRRFHYDIKIFNQWLTEAEQFLRKTQIPENWEHAKYKWYLKELQDGIGQRQTVVRTLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLSDRKKRLEEQKNILSEFQRDLNEFVLWLEEADNIASIPLEPGKEQQLKEKLEQVKLLVEELPLRQGILKQLNETGGPVLVSAPISPEEQDKLENKLKQTNLQWIKVSRALPEKQGEIEAQIKDLGQLEKKLEDLEEQLNHLLLWLSPIRNQLEIYNQPNQEGPFDVKETEIAVQAKQPDVEEILSKGQHLYKEKPATQPVKRKLEDLSSEWKAVNRLLQELRAKQPDLAPGLTTIGASPTQTVTLVTQPVVTKETAISKLEMPSSLMLEVPALADFNRAWTELTDWLSLLDQVIKSQRVMVGDLEDINEMIIKQKATMQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQNQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEGPYTVDAIQKKITETKQLAKDLRQWQTNVDVANDLALKLLRDYSADDTRKVHMITENINASWRSIHKRVSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATRKERLLEDSKGVKELMKQWQDLQGEIEAHTDVYHNLDENSQKILRSLEGSDDAVLLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTEWEKLNLHSADWQRKIDETLERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEIAPLKENVSHVNDLARQLTTLGIQLSPYNLSTLEDLNTRWKLLQVAVEDRVRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQIINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSDSMGEEDLLSPPQDTSTGLEEVMEQLNNSFPSSRGRNTPGKPMREDTM DIP protein P11532 original sequence version: 199 24FF7E83F1E6BF8D arhg5_human Rho guanine nucleotide exchange factor 5 Guanine nucleotide regulatory protein TIM Oncogene TIM p60 TIM Transforming immortalized mammary oncogene ARHGEF5 TIM Ephexin-3 protein protein human Homo sapiens Human MEAEEAQRGASPPISAIEEFSIIPEAPMRSSQVSALGLEAQEDEDPSYKWREEHRLSATQQSELRDVCDYAIETMPSFPKEGSADVEPNQESLVAEACDTPEHWEAVPQSLAGRQARTLAPPELWACPIQSEHLDMAPFSSDLGSEEEEVEFWPGLTSLTLGSGQAEEEEETSSDNSGQTRYYSPCEEHPAETNQNEGSESGTIRQGEELPPEELQESQGLLHPQEVQVLEEQGQQEAGFRGEGTLREDVCADGLLGEEQMIEQVNDEKGEQKQKQEQVQDVMLGRQGERMGLTGEPEGLNDGEWEQEDMERKAQGQGGPEQGEERKRELQVPEENRADSQDEKSQTFLGKSEEVTGKQEDHGIKEKGVPVSGQEAKEPESWDGGRLGAVGRARSREEENEHHGPSMPALIAPEDSPHCDLFPGASYLMTQIPGTQTESRAEELSPAALSPSLEPIRCSHQPISLLGSFLTEESPDKEIDQNSQQEESRLRKGTVSSQGTEVVFASASVTPPRTPDSAPPSPAEAYPITPASVSARPPVAFPRRETSCAARAPETASAPLSMDDPSPCGTSEMCPAALYGFPSTGTSPPRPPANSTGTVQHLRSDSFPGSHRTEQTPDLVGMLLSYSHSELPQRPPKPAIYSSVTPRRDRRSGRDYSTVSASPTALSTLKQDSQESISNLERPSSPPSIQPWVSPHNPAFATESPAYGSSPSFVSMEDVRIHEPLPPPPPQRRDTHPSVVETDGHARVVVPTLKQHSHPPPLALGSGLHAPHKGPLPQASDPAVARQHRPLPSTPDSSHHAQATPRWRYNKPLPPTPDLPQPHLPPISAPGSSRIYRPLPPLPIIDPPTEPPPLPPKSRGRSRSTRGGHMNSGGHAKTRPACQDWTVPLPASAGRTSWPPATARSTESFTSTSRSKSEVSPGMAFSNMTNFLCPSSPTTPWTPELQGPTSKDEAGVSEHPEAPAREPLRRTTPQQGASGPGRSPVGQARQPEKPSHLHLEKASSWPHRRDSGRPPGDSSGQAVAPSEGANKHKGWSRQGLRRPSILPEGSSDSRGPAVEKHPGPSDTVVFREKKPKEVMGGFSRRCSKLINSSQLLYQEYSDVVLNKEIQSQQRLESLSETPGPSSPRQPRKALVSSESYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEVKFELIVSEASYLRSLNIAVDHFQLSTSLRATLSNQEHQWLFSRLQDVRDVSATFLSDLEENFENNIFSFQVCDVVLNHAPDFRRVYLPYVTNQTYQERTFQSLMNSNSNFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNILKRTQPGSSEEAEATKAHHALEQLIRDCNNNVQSMRRTEELIYLSQKIEFECKIFPLISQSRWLVKSGELTALEFSASPGLRRKLNTRPVHLHLFNDCLLLSRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKNLFRLFLRQNTQGAQAEFLFRTETQSEKLRWISALAMPREELDLLECYNSPQVQCLRAYKPRENDELALEKADVVMVTQQSSDGWLEGVRLSDGERGWFPVQQVEFISNPEVRAQNLKEAHRVKTAKLQLVEQQA 93EC1B1D7E1198C5 sntb2_mouse Beta-2-syntrophin Sntb2 Snt2b2 59 kDa dystrophin-associated protein A1 basic component 2 Syntrophin-3 Syntrophin-like protein protein mouse Mus musculus Mouse MAVWTRATKAGLVELLLRERWVRVVAELSGESLSLTGDAAAVEPEPPAAAFNGLPNGGGGESLPGSPNRGLGPPSPPAPPRGPAGEASASPPVRRVRVVKQEAGGLGISIKGGRENRMPILISKIFPGLAADQSRALRLGDAILSVNGTDLRQATHDQAVQALKRAGKEVLLEVKFIREVTPYIKKPSLVSDLPWEGASPQSPSFSGSEDSGSPKHQNTTKDRKVIPLKMCFAARNLSMPDLENRLIELHSPDSRNTLILRCKDTATAHSWFVAIHTNIMALLPQVLAELNAMLGATSTAGGSKEVKHIAWLAEQAKLDGGRQQWRPVLMAVTEKDLLLYDCMPWTRDAWASPCHSYPLVATRLVHSGSGCRSPSLGSDLTFATRTGSRQGIEMHLFRVETHRDLSTWTRILVQGCHAAAELIKEVSLGCTLSGQEVRFTVHYEHGFTITRDNGGASSILYRYPFERLKMSADDGIRNLYLDFGGPEGELTMDLHSCPKPIVFVLHTFLSAKVTRMGLLV 9F6886837C1CDB20 ctnl1_human Alpha-catulin Catenin alpha-like protein 1 Alpha-catenin-related protein CTNNAL1 protein protein human Homo sapiens Human MAASPGPAGVGGAGAVYGSGSSGFALDSGLEIKTRSVEQTLLPLVSQITTLINHKDNTKKSDKTLQAIQRVGQAVNLAVGRFVKVGEAIANENWDLKEEINIACIEAKQAGETIAALTDITNLNHLESDGQITIFTDKTGVIKAARLLLSSVTKVLLLADRVVIKQIITSRNKVLATMERLEKVNSFQEFVQIFSQFGNEMVEFAHLSGDRQNDLKDEKKKAKMAAARAVLEKCTMMLLTASKTCLRHPNCESAHKNKEGVFDRMKVALDKVIEIVTDCKPNGETDISSISIFTGIKEFKMNIEALRENLYFQSKENLSVTLEVILERMEDFTDSAYTSHEHRERILELSTQARMELQQLISVWIQAQSKKTKSIAEELELSILKISHSLNELKKELHSTATQLAADLLKYHADHVVLKALKLTGVEGNLEALAEYACKLSEQKEQLVETCRLLRHISGTEPLEITCIHAEETFQVTGQQIISAAETLTLHPSSKIAKENLDVFCEAWESQISDMSTLLREINDVFEGRRGEKYGYLSLPKPMKNNANLKSLKPDKPDSEEQAKIAKLGLKLGLLTSDADCEIEKWEDQENEIVQYGRNMSSMAYSLYLFTRGEGPLKTSQDLIHQLEVFAAEGLKLTSSVQAFSKQLKDDDKLMLLLEINKLIPLCHQLQTVTKTSLQNKVFLKVDKCITKTRSMMALLVQLLSLCYKLLKKLQMENNGWVSVTNKDTMDSKT DIP protein Q9UBT7 original sequence version: 121 8756237C73AAEA31 utrn_human Utrophin UTRN DMDL Dystrophin-related protein 1 DRP1 protein protein human Homo sapiens Human MAKYGEHEASPDNGQNEFSDIIKSRSDEHNDVQKKTFTKWINARFSKSGKPPINDMFTDLKDGRKLLDLLEGLTGTSLPKERGSTRVHALNNVNRVLQVLHQNNVELVNIGGTDIVDGNHKLTLGLLWSIILHWQVKDVMKDVMSDLQQTNSEKILLSWVRQTTRPYSQVNVLNFTTSWTDGLAFNAVLHRHKPDLFSWDKVVKMSPIERLEHAFSKAQTYLGIEKLLDPEDVAVQLPDKKSIIMYLTSLFEVLPQQVTIDAIREVETLPRKYKKECEEEAINIQSTAPEEEHESPRAETPSTVTEVDMDLDSYQIALEEVLTWLLSAEDTFQEQDDISDDVEEVKDQFATHEAFMMELTAHQSSVGSVLQAGNQLITQGTLSDEEEFEIQEQMTLLNARWEALRVESMDRQSRLHDVLMELQKKQLQQLSAWLTLTEERIQKMETCPLDDDVKSLQKLLEEHKSLQSDLEAEQVKVNSLTHMVVIVDENSGESATAILEDQLQKLGERWTAVCRWTEERWNRLQEINILWQELLEEQCLLKAWLTEKEEALNKVQTSNFKDQKELSVSVRRLAILKEDMEMKRQTLDQLSEIGQDVGQLLDNSKASKKINSDSEELTQRWDSLVQRLEDSSNQVTQAVAKLGMSQIPQKDLLETVRVREQAITKKSKQELPPPPPPKKRQIHVDIEAKKKFDAISAELLNWILKWKTAIQTTEIKEYMKMQDTSEMKKKLKALEKEQRERIPRADELNQTGQILVEQMGKEGLPTEEIKNVLEKVSSEWKNVSQHLEDLERKIQLQEDINAYFKQLDELEKVIKTKEEWVKHTSISESSRQSLPSLKDSCQRELTNLLGLHPKIEMARASCSALMSQPSAPDFVQRGFDSFLGRYQAVQEAVEDRQQHLENELKGQPGHAYLETLKTLKDVLNDSENKAQVSLNVLNDLAKVEKALQEKKTLDEILENQKPALHKLAEETKALEKNVHPDVEKLYKQEFDDVQGKWNKLKVLVSKDLHLLEEIALTLRAFEADSTVIEKWMDGVKDFLMKQQAAQGDDAGLQRQLDQCSAFVNEIETIESSLKNMKEIETNLRSGPVAGIKTWVQTRLGDYQTQLEKLSKEIATQKSRLSESQEKAANLKKDLAEMQEWMTQAEEEYLERDFEYKSPEELESAVEEMKRAKEDVLQKEVRVKILKDNIKLLAAKVPSGGQELTSELNVVLENYQLLCNRIRGKCHTLEEVWSCWIELLHYLDLETTWLNTLEERMKSTEVLPEKTDAVNEALESLESVLRHPADNRTQIRELGQTLIDGGILDDIISEKLEAFNSRYEDLSHLAESKQISLEKQLQVLRETDQMLQVLQESLGELDKQLTTYLTDRIDAFQVPQEAQKIQAEISAHELTLEELRRNMRSQPLTSPESRTARGGSQMDVLQRKLREVSTKFQLFQKPANFEQRMLDCKRVLDGVKAELHVLDVKDVDPDVIQTHLDKCMKLYKTLSEVKLEVETVIKTGRHIVQKQQTDNPKGMDEQLTSLKVLYNDLGAQVTEGKQDLERASQLARKMKKEAASLSEWLSATETELVQKSTSEGLLGDLDTEISWAKNVLKDLEKRKADLNTITESSAALQNLIEGSEPILEERLCVLNAGWSRVRTWTEDWCNTLMNHQNQLEIFDGNVAHISTWLYQAEALLDEIEKKPTSKQEEIVKRLVSELDDANLQVENVRDQALILMNARGSSSRELVEPKLAELNRNFEKVSQHIKSAKLLIAQEPLYQCLVTTETFETGVPFSDLEKLENDIENMLKFVEKHLESSDEDEKMDEESAQIEEVLQRGEEMLHQPMEDNKKEKIRLQLLLLHTRYNKIKAIPIQQRKMGQLASGIRSSLLPTDYLVEINKILLCMDDVELSLNVPELNTAIYEDFSFQEDSLKNIKDQLDKLGEQIAVIHEKQPDVILEASGPEAIQIRDTLTQLNAKWDRINRMYSDRKGCFDRAMEEWRQFHCDLNDLTQWITEAEELLVDTCAPGGSLDLEKARIHQQELEVGISSHQPSFAALNRTGDGIVQKLSQADGSFLKEKLAGLNQRWDAIVAEVKDRQPRLKGESKQVMKYRHQLDEIICWLTKAEHAMQKRSTTELGENLQELRDLTQEMEVHAEKLKWLNRTELEMLSDKSLSLPERDKISESLRTVNMTWNKICREVPTTLKECIQEPSSVSQTRIAAHPNVQKVVLVSSASDIPVQSHRTSEISIPADLDKTITELADWLVLIDQMLKSNIVTVGDVEEINKTVSRMKITKADLEQRHPQLDYVFTLAQNLKNKASSSDMRTAITEKLERVKNQWDGTQHGVELRQQQLEDMIIDSLQWDDHREETEELMRKYEARLYILQQARRDPLTKQISDNQILLQELGPGDGIVMAFDNVLQKLLEEYGSDDTRNVKETTEYLKTSWINLKQSIADRQNALEAEWRTVQASRRDLENFLKWIQEAETTVNVLVDASHRENALQDSILARELKQQMQDIQAEIDAHNDIFKSIDGNRQKMVKALGNSEEATMLQHRLDDMNQRWNDLKAKSASIRAHLEASAEKWNRLLMSLEELIKWLNMKDEELKKQMPIGGDVPALQLQYDHCKALRRELKEKEYSVLNAVDQARVFLADQPIEAPEEPRRNLQSKTELTPEERAQKIAKAMRKQSSEVKEKWESLNAVTSNWQKQVDKALEKLRDLQGAMDDLDADMKEAESVRNGWKPVGDLLIDSLQDHIEKIMAFREEIAPINFKVKTVNDLSSQLSPLDLHPSLKMSRQLDDLNMRWKLLQVSVDDRLKQLQEAHRDFGPSSQHFLSTSVQLPWQRSISHNKVPYYINHQTQTTCWDHPKMTELFQSLADLNNVRFSAYRTAIKIRRLQKALCLDLLELSTTNEIFKQHKLNQNDQLLSVPDVINCLTTTYDGLEQMHKDLVNVPLCVDMCLNWLLNVYDTGRTGKIRVQSLKIGLMSLSKGLLEEKYRYLFKEVAGPTEMCDQRQLGLLLHDAIQIPRQLGEVAAFGGSNIEPSVRSCFQQNNNKPEISVKEFIDWMHLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIVGFRYRSLKHFNYDVCQSCFFSGRTAKGHKLHYPMVEYCIPTTSGEDVRDFTKVLKNKFRSKKYFAKHPRLGYLPVQTVLEGDNLETPITLISMWPEHYDPSQSPQLFHDDTHSRIEQYATRLAQMERTNGSFLTDSSSTTGSVEDEHALIQQYCQTLGGESPVSQPQSPAQILKSVEREERGELERIIADLEEEQRNLQVEYEQLKDQHLRRGLPVGSPPESIISPHHTSEDSELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPESDSRINGVSPWASPQHSALSYSLDPDASGPQFHQAAGEDLLAPPHDTSTDLTEVMEQIHSTFPSCCPNVPSRPQAM DIP protein P46939 original sequence version: 90 DIP protein P46939 original sequence version: 162 DIP protein P46939 original sequence version: 121 C72CADE8CD666993 dtnb_human Dystrobrevin beta DTNB Beta-dystrobrevin protein protein human Homo sapiens Human MIEESGNKRKTMAEKRQLFIEMRAQNFDVIRLSTYRTACKLRFVQKRCNLHLVDIWNMIEAFRDNGLNTLDHTTEISVSRLETVISSIYYQLNKRLPSTHQISVEQSISLLLNFMIAAYDSEGRGKLTVFSVKAMLATMCGGKMLDKLRYVFSQMSDSNGLMIFSKFDQFLKEVLKLPTAVFEGPSFGYTEHSVRTCFPQQRKIMLNMFLDTMMADPPPQCLVWLPLMHRLAHVENVFHPVECSYCRCESMMGFRYRCQQCHNYQLCQNCFWRGHAGGPHSNQHQMKEHSSWKSPAKKLSHAISKSLGCVPTREPPHPVFPEQPEKPLDLAHIVPPRPLTNMNDTMVSHMSSGVPTPTKRLQYSQDIPSHLADEHALIASYVARLQHCARVLDSPSRLDEEHRLIARYAARLAAEAGNVTRPPTDLSFNFDANKQQRQLIAELENKNREILQEIQRLRLEHEQASQPTPEKAQQNPTLLAELRLLRQRKDELEQRMSALQESRRELMVQLEELMKLLKEEEQKQAAQATGSPHTSPTHGGGRPMPMPVRSTSAGSTPTHCPQDSLSGVGGDVQEAFAQGTRRNLRNDLLVAADSITNTMSSLVKELHSAEEGAEEEEEKMQNGKDRG 6927EC0BD86D375C lipa1_human Liprin-alpha-1 Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-1 LAR-interacting protein 1 PPFIA1 LIP1 protein protein human Homo sapiens Human MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC C8CF7C20B298BFB1 ctnl1_mouse Alpha-catulin Ctnnal1 Catnal1 Alpha-catenin-related protein Catenin alpha-like protein 1 protein protein mouse Mus musculus Mouse MAASPVPGGGGAGAVHSSNAAGFTFDSGLEIRTRSVEQTLLPLVSQITTLINHKDNTKKSDKTLQAIQRVGQAVNLAVGRFVKVGEAIANENWDLKEEINIACIEAKQAGETIASLTDVTKRSHLESDGQVTILTDKTGVVQAARLLLSSVTKVLLLADRVVIKQIVTSRNKILATMERLEKVNSFQEFVQIFSQFGNEMVEFAHLTGDRQNDLKDEKKKARMAVARAVLEKGTMMLLTASKTCLRHPSCESAHTNKEGVFDRMRVALEKVTEIVTDCRLSGETDSSSVSIFTGIKELKVNIEALRENVCFESKENLSAALEAVLEHVEDFTDSAYTSHEHRERILELSSQARTELQQLLSVWMQTQSRKTKSAAEELELTVLKISHSLDELRRELHCTAMQLAADLLKFHADHVVLKALKVTGVEGNLEALAEYACKLSEQKEQLVETCRLLRHISGTEPLEITCIHAEETFQVTGQQIISAAETLTLHPSSKIAKENLDVFCEAWESQMSDMATLLREISDVFEGRRGERCDHLSLPKPTKNSANLKSLKPDKPDSEEQAKIAKLGLKLGLLSSDADCEIEKWEDEENEIVRHGRNMSRMAYSLYLFTRGEGPLKTSQDLIHFLEVFAAEGLKLTSSVQSFSKQLKDDDKLMLLLEINKLIPLCHQLQTITKTSLQSKVFLKVDKCITKIRSMMTLVVQLLSLCYKLLKKMENNRWGSATNKDTMDGQN 36CF25115E3EF1F3 dtna_human Dystrobrevin alpha Alpha-dystrobrevin Dystrophin-related protein 3 DTNA DRP3 protein protein human Homo sapiens Human MIEDSGKRGNTMAERRQLFAEMRAQDLDRIRLSTYRTACKLRFVQKKCNLHLVDIWNVIEALRENALNNLDPNTELNVSRLEAVLSTIFYQLNKRMPTTHQIHVEQSISLLLNFLLAAFDPEGHGKISVFAVKMALATLCGGKIMDKLRYIFSMISDSSGVMVYGRYDQFLREVLKLPTAVFEGPSFGYTEQSARSCFSQQKKVTLNGFLDTLMSDPPPQCLVWLPLLHRLANVENVFHPVECSYCHSESMMGFRYRCQQCHNYQLCQDCFWRGHAGGSHSNQHQMKEYTSWKSPAKKLTNALSKSLSCASSREPLHPMFPDQPEKPLNLAHIVDTWPPRPVTSMNDTLFSHSVPSSGSPFITRSSPPKDSEVEQNKLLARAAPAFLKGKGIQYSLNVADRLADEHVLIGLYVNMLRNNPSCMLESSNRLDEEHRLIARYAARLAAESSSSQPPQQRSAPDISFTIDANKQQRQLIAELENKNREILQEIQRLRLEHEQASQPTPEKAQQNPTLLAELRLLRQRKDELEQRMSALQESRRELMVQLEGLMKLLKTQGAGSPRSSPSHTISRPIPMPIRSASACSTPTHTPQDSLTGVGGDVQEAFAQSSRRNLRNDLLVAADSITNTMSSLVKELNSEVGSETESNVDSEFARTQFEDLVPSPTSEKAFLAQIHARKPGYIHSGATTSTMRGDMVTEDADPYVQPEDENYENDSVRQLENELQMEEYLKQKLQDEAYQVSLQG CFD77DAA57D050EC sntb1_human Beta-1-syntrophin SNTB1 SNT2B1 59 kDa dystrophin-associated protein A1 basic component 1 Tax interaction protein 43 Syntrophin-2 BSYN2 protein protein human Homo sapiens Human MAVAAAAAAAGPAGAGGGRAQRSGLLEVLVRDRWHKVLVNLSEDALVLSSEEGAAAYNGIGTATNGSFCRGAGAGHPGAGGAQPPDSPAGVRTAFTDLPEQVPESISNQKRGVKVLKQELGGLGISIKGGKENKMPILISKIFKGLAADQTQALYVGDAILSVNGADLRDATHDEAVQALKRAGKEVLLEVKYMREATPYVKKGSPVSEIGWETPPPESPRLGGSTSDPPSSQSFSFHRDRKSIPLKMCYVTRSMALADPENRQLEIHSPDAKHTVILRSKDSATAQAWFSAIHSNVNDLLTRVIAEVREQLGKTGIAGSREIRHLGWLAEKVPGESKKQWKPALVVLTEKDLLIYDSMPRRKEAWFSPVHTYPLLATRLVHSGPGKGSPQAGVDLSFATRTGTRQGIETHLFRAETSRDLSHWTRSIVQGCHNSAELIAEISTACTYKNQECRLTIHYENGFSITTEPQEGAFPKTIIQSPYEKLKMSSDDGIRMLYLDFGGKDGEIQLDLHSCPKPIVFIIHSFLSAKITRLGLVA BF8E7B416D5CF289 snta1_human Alpha-1-syntrophin 59 kDa dystrophin-associated protein A1 acidic component 1 Pro-TGF-alpha cytoplasmic domain-interacting protein 1 Syntrophin-1 SNTA1 SNT1 protein protein human Homo sapiens Human MASGRRAPRTGLLELRAGAGSGAGGERWQRVLLSLAEDVLTVSPADGDPGPEPGAPREQEPAQLNGAAEPGAGPPQLPEALLLQRRRVTVRKADAGGLGISIKGGRENKMPILISKIFKGLAADQTEALFVGDAILSVNGEDLSSATHDEAVQVLKKTGKEVVLEVKYMKDVSPYFKNSTGGTSVGWDSPPASPLQRQPSSPGPTPRNFSEAKHMSLKMAYVSKRCTPNDPEPRYLEICSADGQDTLFLRAKDEASARSWATAIQAQVNTLTPRVKDELQALLAATSTAGSQDIKQIGWLTEQLPSGGTAPTLALLTEKELLLYLSLPETREALSRPARTAPLIATRLVHSGPSKGSVPYDAELSFALRTGTRHGVDTHLFSVESPQELAAWTRQLVDGCHRAAEGVQEVSTACTWNGRPCSLSVHIDKGFTLWAAEPGAARAVLLRQPFEKLQMSSDDGASLLFLDFGGAEGEIQLDLHSCPKTIVFIIHSFLSAKVTRLGLLA DIP protein Q13424 original sequence version: 78 C07DA5F21C775CF8 snta1_mouse Alpha-1-syntrophin Snta1 Snt1 59 kDa dystrophin-associated protein A1 acidic component 1 Syntrophin-1 protein protein mouse Mus musculus Mouse MASGRRAPRTGLLELRCGAGSGAGGERWQRVLLSLAEDALTVSPADGEPGPEPEPAQLNGAAEPGAAPPQLPEALLLQRRRVTVRKADAGGLGISIKGGRENKMPILISKIFKGLAADQTEALFVGDAILSVNGEDLSSATHDEAVQALKKTGKEVVLEVKYMKEVSPYFKNSAGGTSVGWDSPPASPLQRQPSSPGPQPRNLSEAKHVSLKMAYVSRRCTPTDPEPRYLEICAADGQDAVFLRAKDEASARSWAGAIQAQIGTFIPWVKDELQALLTATGTAGSQDIKQIGWLTEQLPSGGTAPTLALLTEKELLFYCSLPQSREALSRPTRTAPLIATSSAHRLVHSGPSKGSVPYDAELSFALRTGTRHGVDTHLFSVESPQELAAWTRQLVDGCHRAAEGIQEVSTACTWNGRPCSLSVHIDKGFTLWAAEPGAARAMLLRQPFEKLQMSSDDGTSLLFLDFGGAEGEIQLDLHSCPKTMVFIIHSFLSAKVTRLGLLA DIP protein Q61234 original sequence version: 86 161BA76CAF50AE96 ada1d_human Alpha-1D adrenergic receptor Alpha-1D adrenoreceptor Alpha-1A adrenergic receptor Alpha-adrenergic receptor 1a ADRA1D ADRA1A protein protein human Homo sapiens Human MTFRDLLSVSFEGPRPDSSAGGSSAGGGGGSAGGAAPSEGPAVGGVPGGAGGGGGVVGAGSGEDNRSSAGEPGSAGAGGDVNGTAAVGGLVVSAQGVGVGVFLAAFILMAVAGNLLVILSVACNRHLQTVTNYFIVNLAVADLLLSATVLPFSATMEVLGFWAFGRAFCDVWAAVDVLCCTASILSLCTISVDRYVGVRHSLKYPAIMTERKAAAILALLWVVALVVSVGPLLGWKEPVPPDERFCGITEEAGYAVFSSVCSFYLPMAVIVVMYCRVYVVARSTTRSLEAGVKRERGKASEVVLRIHCRGAATGADGAHGMRSAKGHTFRSSLSVRLLKFSREKKAAKTLAIVVGVFVLCWFPFFFVLPLGSLFPQLKPSEGVFKVIFWLGYFNSCVNPLIYPCSSREFKRAFLRLLRCQCRRRRRRRPLWRVYGHHWRASTSGLRQDCAPSSGDAPPGAPLALTALPDPDPEPPGTPEMQAPVASRRKPPSAFREWRLLGPFRRPTTQLRAKVSSLSHKIRAGGAQRAEAACAQRSEVEAVSLGVPHEVAEGATCQAYELADYSNLRETDI EBEB134CF20A4988 dtna_mouse Dystrobrevin alpha Dtna Dtn Alpha-dystrobrevin protein protein mouse Mus musculus Mouse MIEDSGKRGNTMAERRQLFAEMRAQDLDRIRLSTYRTACKLRFVQKKCNLHLVDIWNVIEALRENALNNLDPNIELNVARLEAVLSTIFYQLNKRMPTTHQIHVEQSISLLLNFLLAAFDPEGHGKISVFAVKMALATLCGGKIMDKLRYIFSMISDSSGVMVYGRYDQFLREVLKLPTAVFEGPSFGYTEQSARSCFSQQKKVTLNGFLDTLMSDPPPQCLVWLPLLHRLANVENVFHPVECSYCHSESMMGFRYRCQQCHNYQLCQDCFWRGHAGGSHSNQHQMKEYTSWKSPAKKLTNALSKSLSCASSREPLHPMFPDQPEKPLNLAHIVPPRPVTSMNDTLFSHSVPSSGSPFITRSSPPKDSEVEQNKMLARAAPAFLKGRGIQYSLNVADRLADEHVLIGLYVNMLRNDPPCMLESSNRLDEEHRLIARYAARLAAESSSSQPTQQRSAPDISFTIDANKQQRQLIAELENKNREILQEIQRLRVEHEQASQPTPEKAQQNPTLLAELRLLRQRKDELEQRMSALQESRRELMVQLEGLMKLLKEEELKQGTQGASSPRSSPSHTISRPIPMPIRSASACPTPTHTPQDSLTGVGGDVQEAFAQSSRRNLRSDLLVAADSITNTMSSLVKELNSEVASETESTVDSEFSRPQFEDLAPSPTSEKAFLAQIHSRKPGYIHGGAASTTHGDMVPEDGDPYTQPEDGNYENESVRQLENELQLEEYLKQKLQDEAYQVSLQG 6AB7EF3B69250A09 plcb2_human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 PLCB2 Phosphoinositide phospholipase C-beta-2 Phospholipase C-beta-2 protein protein human Homo sapiens Human MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKNDAINPEDFPEPVYKSFLMSLCPRPEIDEIFTSYHAKAKPYMTKEHLTKFINQKQRDSRLNSLLFPPARPDQVQGLIDKYEPSGINAQRGQLSPEGMVWFLCGPENSVLAQDKLLLHHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDCWKGKPPDEEPIITHGFTMTTDIFFKEAIEAIAESAFKTSPYPIILSFENHVDSPRQQAKMAEYCRTIFGDMLLTEPLEKFPLKPGVPLPSPEDLRGKILIKNKKNQFSGPTSSSKDTGGEAEGSSPPSAPAGEGTVWAGEEGTELEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFVSFEFSAQKNRSYVISSFTELKAYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTMDLPMQQNMAVFEFNGQSGYLLKHEFMRRPDKQFNPFSVDRIDVVVATTLSITVISGQFLSERSVRTYVEVELFGLPGDPKRRYRTKLSPSTNSINPVWKEEPFVFEKILMPELASLRVAVMEEGNKFLGHRIIPINALNSGYHHLCLHSESNMPLTMPALFIFLEMKDYIPGAWADLTVALANPIKFFSAHDTKSVKLKEAMGGLPEKPFPLASPVASQVNGALAPTSNGSPAARAGAREEAMKEAAEPRTASLEELRELKGVVKLQRRHEKELRELERRGARRWEELLQRGAAQLAELGPPGVGGVGACKLGPGKGSRKKRSLPREESAGAAPGEGPEGVDGRVRELKDRLELELLRQGEEQYECVLKRKEQHVAEQISKMMELAREKQAAELKALKETSENDTKEMKKKLETKRLERIQGMTKVTTDKMAQERLKREINNSHIQEVVQVIKQMTENLERHQEKLEEKQAACLEQIREMEKQFQKEALAEYEARMKGLEAEVKESVRACLRTCFPSEAKDKPERACECPPELCEQDPLIAKADAQESRL DIP protein Q00722 original sequence version: 65 A0397309D943672A sntb1_mouse Beta-1-syntrophin Sntb1 Snt2b1 59 kDa dystrophin-associated protein A1 basic component 1 Syntrophin-2 protein protein mouse Mus musculus Mouse MAVAAAAVAAPAGGGGARAQRSGLLEVLVRDRWHKVLVNLSEDALVLSCEEGAAAYNGIGAATNGSFCRGSGTGHPVPGVAQAPDSPAGVRTAFTDLPEQVPESISNQKRGVKVLKQELGGLGISIKGGKENKMPILISKIFKGLAADQTQALYVGDAILSVNGADLRDATHDEAVQALKRAGKEVLLEVKYMREATPYVKKGSPVSEIGWETPPPESPRLGGGSAEPLSSQSFSFHRDRKSIPLKMCYVTRNMTLADPENRQLEIHSPDAKHTVILRSKDSATAQAWFSAIHSNAGDLLTRVVADIREQLGKTGIAGSREIRHLGWLAEKVPGESEKQWKPALVVLTEKDLLIYDSMPRRKEAWFSPVHSYPLLATRLVHSGPGKGSPQAGMDLSFATRTGTKQGIETHLFRAEISRDLSHWTRSIVQGCHNSAELTAEITTACTYRNQECRLTIHYDNGFSISTEPQDGAFPKTIIQSPYEKLKMSSDDGIRMLYLDFGGKEGEIQLDLHSCPKPIVFIIHSFLSAKITRLGLVA 91A5E4DD0A97A665 adra1d-acta2-3 1 12 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 21 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus cam tag calmodulin binding protein tag cam tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 17 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 18 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 13 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Figure 1A, Table S1 adra1d-ctnnal1-11 1 14 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 17 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 18 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 19 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 13 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 12 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 20 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus cam tag calmodulin binding protein tag cam tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association Figure 1A-2, Table S1 adra1d-ctnnal1-12 1 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 17 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 13 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 18 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 19 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 12 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 24 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus cam tag calmodulin binding protein tag cam tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Figure 1A, table S1 sntb1-ctnnal1-1 1 12 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 14 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 17 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 13 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus cam tag calmodulin binding protein tag cam tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Figure 1A, Supp. Table 1. sntb2-snta1-1 1 18 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 12 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 19 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 22 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus cam tag calmodulin binding protein tag cam tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 15 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 17 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 13 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level association association Figure 1A, Supp. Table 1 adra1d-ctnnal1-13 4 17 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 12 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 21 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus cam tag calmodulin binding protein tag cam tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association Figure 1B Unidentified Syntrophins were also detected. Both endogenous a-catulin and transfected flag-tagged a-catulin bound to TAP-a1D-ARs, adra1d-ctnnal1-14 4 12 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 17 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 12 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 21 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus cam tag calmodulin binding protein tag cam tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association Figure 1Bi Unidentified Syntrophins were also detected. adra1d-ctnnal1-15 4 12 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 12 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 22 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus cam tag calmodulin binding protein tag cam tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association Figure 2A Unidentified Syntrophins were also detected. AP-a-DB1 interacts with both endogenous and transiently transfected a-catulin-flag, whereas TAP-a-DB2 (with shorter C-terminal sequence) failed to coimmunoprecipitate either protein. AP-a-DB1 interacts with both endogenous and transiently transfected α-catulin-flag, whereas TAP-a-DB2 failed to coimmunoprecipitate either protein dtna-ctnnal1-1 4 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 12 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 22 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus cam tag calmodulin binding protein tag cam tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association Figure 2Ai Unidentified Syntrophins were also detected. AP-a-DB1 interacts with both endogenous and transiently transfected a-catulin-flag, whereas TAP-a-DB2 (with shorter C-terminal sequence) failed to coimmunoprecipitate either protein. ctnnal1-dmd-5 3 12 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 13 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 17 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level association association Figure 2B Unidentified Syntropins were also detected. a-catulin-flag coimmunoprecipitates specifically with endogenous a-DB1 but not a-DB2 (with shorter C-terminus). dtna-ctnnal1-2 6 12 anti tag western anti tag western blot anti tag western anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 17 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 22 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate n-terminus strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus cam tag calmodulin binding protein tag cam tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. association association Figure 2C GST-a-catulin bound endogenous and TAP-a-DB1 but failed to bind either endogenous or TAP-a-DB2. ctnnal1-dtna-1 6 12 unspecified role unspecified role bait bait purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association Figure 2C-2, Supp. figure 3. GST-a-catulin bound endogenous and TAP-a-DB1 but failed to bind either endogenous or TAP-a-DB2. dtna-ctnnal1-3 5 17 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 12 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus cam tag calmodulin binding protein tag cam tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Figure 2D ctnnal1-dtna-3 2 12 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell 17 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell colocalization colocalization Figure 3 Colocalisation was also observed between Syntrophin and alpha-Catulin in 293 cells, but the identity of the Syntropin is not stated, nor the antibody used for staining. ctnnal1-dtna-2 7 16 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell 22 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell colocalization colocalization Figure 3 a-Catulin displays strong codistribution witha-dystrobrevins in nerve bundles and blood vessels. ctnnal1-dtna-4 1 10 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 13 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 15 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 18 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 19 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 23 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 17 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 14 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 12 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus cam tag calmodulin binding protein tag cam tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association Figure 4C (Table S2) ctnnal1-arhgef5-1 4 23 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 12 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus cam tag calmodulin binding protein tag cam tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Figure 4D a faint band was visible. ctnnal1-plcb2-1 6 23 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 12 unspecified role unspecified role bait bait purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association Figure 4E a faint band was visible. dtna-ctnnal1-4 4 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 23 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 12 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 22 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus cam tag calmodulin binding protein tag cam tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association Figure 4F Unidentified Syntrophins were also detected. An unidentified isoform of Dystrophin is stated to have been detected -p71. there are several Dystropin isoforms with Dp71 in the name - no antibody used is listed either.