IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit. Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit. The Biochemical Journal 2010 imex curation Dennerlein S., Rozanska A., Wydro M., Chrzanowska-Lightowlers ZM., Lightowlers RN. The anti tag-mrps27 coip of figure 1C and anti-tag-itc1 of figure 2C cannot be annotated because it is not mentioned if the construct is of human origin and authors did not answer a request for information. The anti tag-mrps27 coip of figure 1C and anti-tag-itc1 of figure 2C cannot be annotated because it is not mentioned if the construct is human and authors do not answer. z.chrzanowska-lightowlers@ncl.ac.uk 21-Nov-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Dennerlein S., Rozanska A., Wydro M., Chrzanowska-Lightowlers ZM., Lightowlers RN. The anti tag-mrps27 coip of figure 1C and anti-tag-itc1 of figure 2C cannot be annotated because it is not mentioned if the construct is of human origin and authors did not answer a request for information. The anti tag-mrps27 coip of figure 1C and anti-tag-itc1 of figure 2C cannot be annotated because it is not mentioned if the construct is human and authors do not answer. z.chrzanowska-lightowlers@ncl.ac.uk Accepted 2019-OCT-29 AT 14:10 GMT AT 14:10 GMT by MGT The Biochemical Journal 2010 Only protein-protein interactions imex curation imex curation Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit. Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit. The Biochemical Journal 2010 imex curation Dennerlein S., Rozanska A., Wydro M., Chrzanowska-Lightowlers ZM., Lightowlers RN. The anti tag-mrps27 coip of figure 1C and anti-tag-itc1 of figure 2C cannot be annotated because it is not mentioned if the construct is of human origin and authors did not answer a request for information. The anti tag-mrps27 coip of figure 1C and anti-tag-itc1 of figure 2C cannot be annotated because it is not mentioned if the construct is human and authors do not answer. z.chrzanowska-lightowlers@ncl.ac.uk 21-Nov-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 cosedimentation cosedimentation western blot western blot Immuno blot Immuno blot Dennerlein S., Rozanska A., Wydro M., Chrzanowska-Lightowlers ZM., Lightowlers RN. The anti tag-mrps27 coip of figure 1C and anti-tag-itc1 of figure 2C cannot be annotated because it is not mentioned if the construct is of human origin and authors did not answer a request for information. The anti tag-mrps27 coip of figure 1C and anti-tag-itc1 of figure 2C cannot be annotated because it is not mentioned if the construct is human and authors do not answer. z.chrzanowska-lightowlers@ncl.ac.uk Accepted 2019-OCT-29 AT 14:10 GMT AT 14:10 GMT by MGT The Biochemical Journal 2010 Only protein-protein interactions imex curation imex curation Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit. Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit. The Biochemical Journal 2010 imex curation Dennerlein S., Rozanska A., Wydro M., Chrzanowska-Lightowlers ZM., Lightowlers RN. The anti tag-mrps27 coip of figure 1C and anti-tag-itc1 of figure 2C cannot be annotated because it is not mentioned if the construct is of human origin and authors did not answer a request for information. The anti tag-mrps27 coip of figure 1C and anti-tag-itc1 of figure 2C cannot be annotated because it is not mentioned if the construct is human and authors do not answer. z.chrzanowska-lightowlers@ncl.ac.uk 21-Nov-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human Homo sapiens Human clip clip cross linking immunoprecipitation CLIP cross-linking immunoprecipitation clip nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Dennerlein S., Rozanska A., Wydro M., Chrzanowska-Lightowlers ZM., Lightowlers RN. The anti tag-mrps27 coip of figure 1C and anti-tag-itc1 of figure 2C cannot be annotated because it is not mentioned if the construct is of human origin and authors did not answer a request for information. The anti tag-mrps27 coip of figure 1C and anti-tag-itc1 of figure 2C cannot be annotated because it is not mentioned if the construct is human and authors do not answer. z.chrzanowska-lightowlers@ncl.ac.uk Accepted 2019-OCT-29 AT 14:10 GMT AT 14:10 GMT by MGT The Biochemical Journal 2010 Only protein-protein interactions imex curation imex curation rm12_human 39S ribosomal protein L12, mitochondrial MRPL12 RPML12 5c5-2 MRPL7 Mitochondrial large ribosomal subunit protein bL12m protein protein human Homo sapiens Human MLPAAARPLWGPCLGLRAAAFRLARRQVPCVCAVRHMRSSGHQRCEALAGAPLDNAPKEYPPKIQQLVQDIASLTLLEISDLNELLKKTLKIQDVGLVPMGGVMSGAVPAAAAQEAVEEDIPIAKERTHFTVRLTEAKPVDKVKLIKEIKNYIQGINLVQAKKLVESLPQEIKANVAKAEAEKIKAALEAVGGTVVLE DIP protein P52815 original sequence version: 121 7F26BEDE9218CEF0 rt25_human 28S ribosomal protein S25, mitochondrial MRPS25 RPMS25 Mitochondrial small ribosomal subunit protein mS25 protein protein human Homo sapiens Human MPMKGRFPIRRTLQYLSQGNVVFKDSVKVMTVNYNTHGELGEGARKFVFFNIPQIQYKNPWVQIMMFKNMTPSPFLRFYLDSGEQVLVDVETKSNKEIMEHIRKILGKNEETLREEEEEKKQLSHPANFGPRKYCLRECICEVEGQVPCPSLVPLPKEMRGKYKAALKADAQD 78BB282C1539FA4C rt18b_human 28S ribosomal protein S18b, mitochondrial 28S ribosomal protein S18-2, mitochondrial C6orf14 MRPS18B Mitochondrial small ribosomal subunit protein bS18b Mitochondrial small ribosomal subunit protein mS40 HSPC183 PTD017 protein protein human Homo sapiens Human MAASVLNTVLRRLPMLSLFRGSHRVQVPLQTLCTKAPSEEDSLSSVPISPYKDEPWKYLESEEYQERYGSRPVWADYRRNHKGGVPPQRTRKTCIRRNKVVGNPCPICRDHKLHVDFRNVKLLEQFVCAHTGIIFYAPYTGVCVKQHKRLTQAIQKARDHGLLIYHIPQVEPRDLDFSTSHGAVSATPPAPTLVSGDPWYPWYNWKQPPERELSRLRRLYQGHLQEESGPPPESMPKMPPRTPAEASSTGQTGPQSAL DIP protein Q9Y676 original sequence version: 56 DIP protein Q9Y676 original sequence version: 97 B4C83E5593796C5D rm03_human 39S ribosomal protein L3, mitochondrial Mitochondrial large ribosomal subunit protein uL3m MRPL3 MRL3 RPML3 protein protein human Homo sapiens Human MPGWRLLTQVGAQVLGRLGDGLGAALGPGNRTHIWLFVRGLHGKSGTWWDEHLSEENVPFIKQLVSDEDKAQLASKLCPLKDEPWPIHPWEPGSFRVGLIALKLGMMPLWTKDGQKHVVTLLQVQDCHVLKYTSKENCNGKMATLSVGGKTVSRFRKATSILEFYRELGLPPKQTVKIFNITDNAAIKPGTPLYAAHFRPGQYVDVTAKTIGKGFQGVMKRWGFKGQPATHGQTKTHRRPGAVATGDIGRVWPGTKMPGKMGNIYRTEYGLKVWRINTKHNIIYVNGSVPGHKNCLVKVKDSKLPAYKDLGKNLPFPTYFPDGDEEELPEDLYDENVCQPGAPSITFA 6DE6B5BD6BD72E32 rt29_human 28S ribosomal protein S29, mitochondrial DAP3 MRPS29 Death-associated protein 3 Ionizing radiation resistance conferring protein Mitochondrial small ribosomal subunit protein mS29 protein protein human Homo sapiens Human MMLKGITRLISRIHKLDPGRFLHMGTQARQSIAAHLDNQVPVESPRAISRTNENDPAKHGDQHEGQHYNISPQDLETVFPHGLPPRFVMQVKTFSEACLMVRKPALELLHYLKNTSFAYPAIRYLLYGEKGTGKTLSLCHVIHFCAKQDWLILHIPDAHLWVKNCRDLLQSSYNKQRFDQPLEASTWLKNFKTTNERFLNQIKVQEKYVWNKRESTEKGSPLGEVVEQGITRVRNATDAVGIVLKELKRQSSLGMFHLLVAVDGINALWGRTTLKREDKSPIAPEELALVHNLRKMMKNDWHGGAIVSALSQTGSLFKPRKAYLPQELLGKEGFDALDPFIPILVSNYNPKEFESCIQYYLENNWLQHEKAPTEEGKKELLFLSNANPSLLERHCAYL 778AF183FA04DEC8 eral1_human GTPase Era, mitochondrial ERAL1 HERA Conserved ERA-like GTPase ERA-W ERA-like protein 1 protein protein human Homo sapiens Human MAAPSWRGARLVQSVLRVWQVGPHVARERVIPFSSLLGFQRRCVSCVAGSAFSGPRLASASRSNGQGSALDHFLGFSQPDSSVTPCVPAVSMNRDEQDVLLVHHPDMPENSRVLRVVLLGAPNAGKSTLSNQLLGRKVFPVSRKVHTTRCQALGVITEKETQVILLDTPGIISPGKQKRHHLELSLLEDPWKSMESADLVVVLVDVSDKWTRNQLSPQLLRCLTKYSQIPSVLVMNKVDCLKQKSVLLELTAALTEGVVNGKKLKMRQAFHSHPGTHCPSPAVKDPNTQSVGNPQRIGWPHFKEIFMLSALSQEDVKTLKQYLLTQAQPGPWEYHSAVLTSQTPEEICANIIREKLLEHLPQEVPYNVQQKTAVWEEGPGGELVIQQKLLVPKESYVKLLIGPKGHVISQIAQEAGHDLMDIFLCDVDIRLSVKLLK 5C2454A1D9AFF5AA rrna 12s mitoch mitochondrially encoded 12S rRNA rrna ribosomal rna rRNA rrna human Homo sapiens Human AAUAGGUUUGGUCCUAGCCUUUCUAUUAGCUCUUAGUAAGAUUACACAUGCAAGCAUCCCCGUUCCAGUGAGUUCACCCUCUAAAUCACCACGAUCAAAAGGAACAAGCAUCAAGCACGCAGCAAUGCAGCUCAAAACGCUUAGCCUAGCCACACCCCCACGGGAAACAGCAGUGAUUAACCUUUAGCAAUAAACGAAAGUUUAACUAAGCUAUACUAACCCCAGGGUUGGUCAAUUUCGUGCCAGCCACCGCGGUCACACGAUUAACCCAAGUCAAUAGAAGCCGGCGUAAAGAGUGUUUUAGAUCACCCCCUCCCCAAUAAAGCUAAAACUCACCUGAGUUGUAAAAAACUCCAGUUGACACAAAAUAGACUACGAAAGUGGCUUUAACAUAUCUGAACACACAAUAGCUAAGACCCAAACUGGGAUUAGAUACCCCACUAUGCUUAGCCCUAAACCUCAACAGUUAAAUCAACAAAACUGCUCGCCAGAACACUACGAGCCACAGCUUAAAACUCAAAGGACCUGGCGGUGCUUCAUAUCCCUCUAGAGGAGCCUGUUCUGUAAUCGAUAAACCCCGAUCAACCUCACCACCUCUUGCUCAGCCUAUAUACCGCCAUCUUCAGCAAACCCUGAUGAAGGCUACAAAGUAAGCGCAAGUACCCACGUAAAGACGUUAGGUCAAGGUGUAGCCCAUGAGGUGGCAAGAAAUGGGCUACAUUUUCUACCCCAGAAAACUACGAUAGCCCUUAUGAAACUUAAGGGUCGAAGGUGGAUUUAGCAGUAAACUAAGAGUAGAGUGCUUAGUUGAACAGGGCCCUGAAGCGCGUACACACCGCCCGUCACCCUCCUCAAGUAUACUUCAAAGGACAUUUAACUAAAACCCCUACGCAUUUAUAUAGAGGAGACAAGUCGUAACAUGGUAAGUGUACUGGAAAGUGCACUUGGACGAAC rt26_human 28S ribosomal protein S26, mitochondrial 28S ribosomal protein S13, mitochondrial MRPS26 RPMS13 C20orf193 Mitochondrial small ribosomal subunit protein mS26 protein protein human Homo sapiens Human MLRALSRLGAGTPCRPRAPLVLPARGRKTRHDPLAKSKIERVNMPPAVDPAEFFVLMERYQHYRQTVRALRMEFVSEVQRKVHEARAGVLAERKALKDAAEHRELMAWNQAENRRLHELRIARLRQEEREQEQRQALEQARKAEEVQAWAQRKEREVLQLQEEVKNFITRENLEARVEAALDSRKNYNWAITREGLVVRPQRRDS F40EF470813840EF dap3-eral1-1 2 8 unspecified role unspecified role neutral component neutral component cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level 9 unspecified role unspecified role neutral component neutral component cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization Fig 1B mrps26-dap3-1 1 4 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 7 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 5 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 9 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 6 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level association association Fig 2B components of this complex (immunoprecipitaded with mrps26) decrease if eral1 is depleted. in particular, mrps25 seems absent. rrna 12s mitoch-eral1-1 3 10 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 9 antibody detection identification by antibody antibody detection nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level association association Fig 3A, Suppl Table 1